SMG1 (AK026175) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LEPR; OB-RGRP; OBRGRP; VPS55
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026175, the custom clone sequence may differ by one or more nucleotides TTGACACCTGCATTGGGCCGACTGCCATTCCCATGACTGCTGCACCTGCGTTTTTAGAGAATGCCTCATA ACCCACTGATTCTCATTCACAGAGAATGGGAGAACGGAATGAAGAAAGATTCCAGCAGCTTATAGAAGGA TAGCAATATTTTGGGACAGGGAAAATCCTGTCATACCTCATCTCTTCCTCAGGAGGAGTTCTGAGCTGGT CCTGCTTTTCATAGTTGTTTCTTTTCTTCCACTTAAGAACTCATAGATTTTTCTTACTGTCCTAAGGAAG TCCTTACCTCTGGGGTATCTCCTCAATGAATACTGTTTTCAAGGCTGAAATAGTTCATTATGTTAATAAC CTTCTTTATGTTCTCAGGGAAATGCTTAGGTGGTGTCACAAAATGTGCCTTTTCTTTTCTTTTCTTTTTT TTTTTTTTGAGGCAGAGTCTCGCTCTGTTGCCCAGGCTGGAGTGCAGTGGTGCGATCTCGGCTCACTGCA AGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCAAGGAGCTGGGACTACAGGCTCCCG CCACCACACCTGGCTAATTTTTTTGTATTTTTAGTAAAGACGGGTTTCATCGTGTTAGCCAGGATGGTCT CGATCTCCTGACCTCATGATCCGCCCGCCTCTGCCTCCCAAAGTGCTGGGATTACAGGCCTGAGCCACTG TGCCCAGCCAAAATGTGCCTTTGCAAAGTTTGCGAAATCAGATTTTGTATCCCAATAGAACCAAAATATT TATGGGGATGCTAGCATTTTCCAAGCATAGTAATTAGTTCACAACTGAGAAATATTATGTCTGTAGTAGA TAAATAGTAGTTGTGCATTTTAATTTAATTCTCCTTTTTCCATTTTGTCTCATGAAGTACCTTATTGCAA AAATCCCACTGAGTAATAGCTCATAAATTATAATCTTTCAAATAGCCATGCTACCAGCGTACAACAGTGA TACATGTAACCCCAAATGTGATGTGAGAGGACGATTACTTTGTAAATAAAACTTGTTATTGACATTTTAA AAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026175 unedited NNNNNGGGGAACGACAGAATTGTATACAACTCACTATAGGCGGCCGCGNAATTCGCACGA GNATCTTTATCATGTTGTCAAACAAGTGGGTTACAAGCAGACTTTGAGACACTTTTCCAC AGAAACAATACTATGAATTGGTGATTGAGTTCCCAGGCCAAGCCTCCCTCAACAGGTTCA ACTCTAATATACCTAACCTGTGATACTGAAGGTGCCTGCCTGAGTTTTGGGTCTCTGAGA CAGGGTAGTGTGAGTAGTTTGGAGGAAGGACAGTGCAACTTTCCACCCCTTTTCCTAAGA ACAAGGTCTTTCTCCTTTTAATTTTTCCACTCATTTTCACCTCCTAATGCCCTTGAGATC CAGGTACACTCCTGGGAGTTTTGTTCACCTCTCCCAACTGAGAACCTTCCCACTGGGCTC CATCCTCCCTCCTGAGGTTCTTCATATTCCAGAGTCACCCACCCTTCTCCTCCCATTAGT CAGTTCTCTAAGTACAGCTGATGTCATGTGGTGCTGAGAAGAAAGCAGATCACACTTCAT CACAGAAAGAATGCCTTGTGATTATCTTCTCCACATCTGAAATTCCTTTTGACACCTGCA TTGNGCCGACTGCCATTCCCATGACTGCTGCACCTGCGTTTTTAGAGAATGCCTCATAAC CCACTGATTCTCATTCACAGAGAATGGGAGAACGGAATGAAGAAAGATTCCAGCAGCTTA TAGAAGGATAGNCATATTTTGGGACAGGGAAAATCCTGTCATACCTCATCTCTTCCTCCA GAGGAGTTCTGAGCTGGTCCTGCTTTTCATAGTTGTTTCTTTTTCTTCACTTAAGACTCA TAGATTTTTTCTACTGTCCTAAGGAAGTCCTTACCTCTGAGGTATCTCCTCATGN
Restriction Sites	NotI-NotI
ACCN	AK026175
Insert Size	1700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK026175.1
RefSeq Size	1069 bp
RefSeq ORF	1069 bp
Locus ID	23049
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	This gene encodes a protein involved in nonsense-mediated mRNA decay (NMD) as part of the mRNA surveillance complex. The protein has kinase activity and is thought to function in NMD by phosphorylating the regulator of nonsense transcripts 1 protein. Alternatively spliced transcript variants have been described, but their full-length nature has yet to be determined. [provided by RefSeq, Mar 2013]

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