Calcium binding protein P22 (CHP1) (AK093949) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK093949, the custom clone sequence may differ by one or more nucleotides GTTGGTTATTTGCCTTTACTTTTATTTATTTGTTTTGATATGTAGATACATTTACATTTTTTATGTAATA TATCTTTTTAAAAATGGTTTCTGCTTTTCCTGAACATGTTTTCAGAATTTAAAATTGTATATGGAAAACA AATTACATGAAAGATTTGAGCATTTCAAAATTTTAAACATAAAAGCATAAACGTAGATAAAATGAAGGTG TACTATGATATCTTCAGTTTTATCAGAAATGATGTAAAAATTACAACCTCTTTAAAAAGTAGTGTTAATC ATTAAGTTAGAAAATATATAGCTGGGCATGGTGGCAGATGCCTGTAATCCCAGCTACATGGGAAGGTGAG GTGGGAGAATCGCTTGAACCCAGGCAGTGGAGGATGCAGTGAGCCAAGATCATGCCACTGCACCCCAGCC TGGGTGACAGAACAAGACTCCATCTCAAGAAAAAAAAAAAAAAAGAAAAATATATATATGAACTTCAGAA TCTGAGGTCATATATAGACAGGTCTTTCCCCCGTTTCCTCTACTTTTTCTTGTAGCTTGGAATTAGTCAG TTTCATCATGCTATAATAAGCTTATCTGAAAGGCAGTAAAGTGATATTTTGTACAACTTCATTGGCTTTT TGAGAAGAACATTTTTAGGTTCTTAGTCCTAGAATTCTGCTGTTTGCTTGGAAAAAGAAAGTAATACATT TTCTTCTATGAAGGATTTCCTCAGCACACCATTGCTTCTTTATCTGACCAGGATGCAAAACCCTCTTTCA GCATGGCGTAAGTAAAGACCTTGAAAATATCAGTAATAATGTTTTTACATTTTTAATTCCTTCTTTTAAG TTATAAATTCAAAAGATAGGCAATGAAGACTATCTCTATATACTTGTATGGAGTGATCTTCAGGATAAAT TACTAAGTAAAACAGTAGTTTGAGAGAATTTTGTAGTATGCTGCTAATCACCTAAGAAGAAAGTAGAGAT GTAAGTGGATGTATATACTTGTTTATGTTAATAATAAAAACAATAGTATGGAAATAATAAAAACTTAAAA GGGGTGGAGGGGGAAATTTTTTTTTTTTAATGTTTACCTCAGTGAGTGTGTAGGGAAAAGCAAAGGGTTA GACCCTAGACTTTTCTGAATGCATCACTTAATGATAGGGACACATTCTGAGAAATGCGTCATTAGGTGAT ACCATCATTGTGCAAACATCACAGAGTGCACTTACACAAACCTAGATAGTGTAGCCTACTAACATCGCAG TTATATAGTATAGCCTATTGCTCCTAGGCCGCACACCTGTACAGCATATTGTGTACTGAATAATGTAGGC AGTTGTAATGTAACACCTAGTGTTTGTTTAACTAAACACAGAAAAGGTACAACTAAAATGTATTATTTTA TGGGACCACTGTCATATATGTGGTTCATCACTGACCAAAATGTTATATAGAATATTACTGTACCTTGTTT TAACTTCAGAGCTTTGTATTTTACATAATTATAAAACAAAATTAAATTTAAGAGAGTAATTCTTAAAAAT CACAAACAAGATTTCTGCCTCCAGCCAAAAAGCTGTAACAGGGACTAGATTTACCCTCCTGTCAAAAAAA GCAGACAAAATATATAAAACAGTGGTTTTCAGACGTTGGACAGCAAGCCTTGCTGATAATGATCCCTGAA AGAAAGGAAACAAATGAGATGGGCCCTAAGAGCACTCCACCTTACTGCCTGGATGGAATTTCCAGGCTGC ACACAGAGGCTGGAGATGTTGTTGAGTTGAAGAAACAAAGTTCATCTTTCAAGGAGGCTGAGATGGCGAT AATTTTGCAGGTCAGAGTACTGGAGAGGAAAGATCTGCACAGAGAGAACTCTCTGGGGAGCTGCATAGAC TTGCTCATGAGTCAGCAGCTGAGCGTTGATCAGCCCTTTTGTGTGAGGAGACTACTGGAAGTCAGGGAAA AAATCACTGGAAAGCAGCAGACAGAACAGTTTCCAGAGCTCACACAGAGAACATTCATGTTCTCACCAGT CTGTATGGAAATACCTCTTAACATGACAGGCATTGAATAGAGTCCACAGTAATGGGATCAAATTAATTCT ATCACAAGAACTGGAACCAGAGGCAAAAAGAAAAGAAAAGAAAAGAAAAATCTAGACTAACAGCTGTTCT AGACCAGCCTACCAAAGTTTAAAAGCAAATTTTGAAGAGATTAAACTATTTCCAAGTTAATGAAGTATAT TTCAGAACAAACCCTTAAATATTTGAGGGAATAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK093949 unedited GCGGCAACAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGTGAGGTGG GAGAATCGCTTGAACCCAGGCAGTGGAGGATGCAGTGAGCCAAGATCATGCCACTGCACC CCAGCCTGGGTGACAGAACAAGACTCCATCTCAAGAAAAAAAAAAAAGAAAAATATATAT ATGAACTTCAGAATCTGAGGTCATATATAGACAGGTCTTTCCCCCGTTTCCTCTACTTTT TCTTGTAGCTTGGAATTAGTCAGTTTCATCATGCTATAATAAGCTTATCTGAAAGGCAGT AAAGTGATATTTTGTACAACTTCATTGGCTTTTTGAGAAGAACATTTTTAGGTTCTTAGT CCTAGAATTCTGCTGTTTGCTTGGAAAAAGAAAGTAATACATTTTCTTCTATGAAGGATT TCCTCAGCACACCATTGCTTCTTTATCTGACCAGGATGCAAAACCCTCTTTCAGCATGGC GTAAGTAAAGACCTTGAAAATATCAGTGATAATGTTTTTACATTTTTAATTCCTTCTTTT AAGTTATAAATTCAAAAGATAGGCAATGAAGACTATCTCTATATACTTGTATGGAGTGAT CTTCAGGATAAATTACTAAGTAAAACAGTAGTTTGAGAGAATNTTGTAGTATGCTGCTAA TCACCTAAGAAGAAAGTAGAGATGTAAGTGGATGTATATACTTGTTTATNGTAATAAATA AAAACATAGTATGGAAATNAATAAAACTTAAAAGGGGTGGGAGGGGAAATTNTTTTTTTT ATGTTTACTCAGTGAGTGTGTAGGGAAAAGCAAGGGNTAGACCCTAGACTNTNCTGATGC ATCACTTATGATAGGGATACATTCCGAGAATGTGTCATTNAGTGATCCATCATTGTGCAA CATCACAGATGCACTA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK093949 unedited NGGGTATATCTTGNACCGCGCCGCAATCTANGATCGGTTTTTTTTTTTTTTTTTTGTATT CCCTCAAATATTTAAGGGTTTGTTCTGAAAATACTTCATTAACTTGGAAATAGTTTAATC TCTTCAAAATTTGCTTTTAAACTTTGTTAGGCTGGTCTAGAACAGCTGTTAGTCTAGATT TTTCTTTTCTTTTCTTTTTGCCTCTGGTTCCAGTTCTTGTGATAGAATTAATTTGATCCC ATTACTGTGGACTCTATTCAATGCCTGTCATGTTAAGAGGTATTTCCATACAGACTGGTG AGAACATGAATGTTCTCTGTGTGAGCTCTGGAAACTGTTCTGTCTGCTGCTTTCCAGTGA TTTTTTCCCTGACTTCCAGTAGTCTCCTCACACAAAAGGGCTGATCAACGCTCAGCTGCT GACTCATGAGCAAGTCTATGCAGCTCCCCAGAGAGTTCTCTCTGTGCAGATCTTTCCTCT CCAGTACTCTGACCTGCAAAATTATCGCCATCTCAGCCTCCTTGAAAGATGAACTTTGTT TCTTCAACTCAACAACATCTCCAGCCTCTGTGTGCAGCCTGGAAATTCCATCCAGGCAGT AAGGTGGAGTGCTCTTAGGGCCCATCTCATTTGTTTCCTTTCTTTCAGGGATCATTATCA GCAGGGCTTGCTGTCCAACGTCTGAAAACCACTGTTTTAATATTTTGGCGGCTTTTTTTG ACAGGGAGGTAAATCTAGTCCCTGTTACAGCTTTTTGCCTGAAGCCAAATCTTGTTGGGG ATTTTAAGAATACCCCCCTAAATTAAATTTGGTTAAAAATATGGAAAAACCAACCTTTGA ATTTAAACC
Restriction Sites	NotI-NotI
ACCN	AK093949
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK093949.1
RefSeq Size	2274 bp
RefSeq ORF	2274 bp
Locus ID	11261
Protein Pathways	Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
Gene Summary	This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity. [provided by RefSeq, Jul 2008]

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