FOXK1 (AK023847) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK023847, the custom clone sequence may differ by one or more nucleotides TACTCACCAGCTCAAAACCTGACTCATCTCCCGTCACCCCACCCACAGACCCAGCCGATGACTCCTCTTC CTTTGACCCCGTGGATTTTCTCCCTCCTCGACAGCATCATGATCCTCCACCAGAGCATCATGATCCTCCA CCGTATGTCCCTGCTCCGGCTCTACCCCTCTCCCCCACTCTCTCCAACCAACCCACTTCTGACTCTGAGA CCTCTCTGCCTCCTCCCCTCACCCGCTCTCGGGCCCAATGTGCTCAGCAACCAGCTCCCTTGCTTCCTCT CCGGGAAGTAGCGGGAGTTGAGGGGATCGTCCATGTCCACGTCCCTTTCTCCTTCTACGATCTCTTACAG ATTGAAGAACGTCTCGGGTCCTTCTCCTCCGATCCTGATACTTACATCAAAGAATTTAAATATCTTACTC AATCTTATGAACTCACTTGGCATGATCTCTACTTTATCCTCTCTTCTACCCTCCTTCCAGAAGAGAAGGA AAGAGTGTGGCTCGCAGCACAGGCATATGCCGACGACCTTCATCAGCAGGATCCTACTAAGCCCGTAGGA GCTGCTGCTGTTCCCTGGGAAGAGCCTTCCTGGGAATACCAACCCACAGAACCTGGCCAAGTGTACTGTA GCCATATGATTACTTGCCTCATTGCAGGCCTTAACAAAGCTGCCCATAGGCTGTAAATTTTGAAAAACTT AAAGACATTTCCCAAAAGGCAGATGAAAATCCTGCCCAATCTCTTTCCCGCCTTACAGAAGCTCTCCAAA AATATACCCGCATCGACCCTGCCTCCTGAGAAGGAACTATTGTTCTTTCTTTAAAAAATTTTATTTATTT TTTAATTATTATTATACTTTAAGTTTTAGGGTACATGTGCACAATGTGCAGGTTTGTTACATATGTAGGA ACTATTGTTCTTAATACCCATTTTATTTCCCAATCTGCCCCTGACATCCGGCACAAACTTAAAAAGGCTG AAGATGGCTCTCAAACCCCACAACAAGACCTCCTTAACCTGGCTTTCAAAGTCTTTAATAACAGGGACGA GCAAAATAAATTAGATAAAGCCCAAAGAGATCGTGCTAAATACCAGCTTCCAGCAGTGGCTATCTGTCAG TCTAGCCATAGTACCCAAGGGCACAAAAGACCTGATAGCAGCAAACCTCCTGGGCCTTGTTTTAAGTGCG GCAAAGAAGGCCACTGGACGGGGGCATGTCCCCACCCATGAGTGCCGAAGAGCCCTTGCCTAGTCTGCCA GCAGATGGGCACTGGAAGTCTGACTGTCCTCTCAACAGATGGGCAGACAAGCATGCTCCTCAGAGCCACC ATCCCTTCAACAAGACAAAAAGTGAAGAATCACTTGCACTCCCATAGCTCCTTGGCCTAGCCACTGAAGA CTGATGAAGCCAGGGCCCCTGGGCCCCGGCTCCCGGCCCCCACTGCCATCACTGCATCGGAGCCCAGGGT AACTCTACTAGTAGCAGGTAAGCTGATCTCTTTTTTAATTGATAGTGGGGCCACCTACTCAGCTTTGCCT GAGTTCTCAGGACCCACTCATCCCTCTCAAGTCTCAGTTGTGGGGGTTGACGGACTCATCTCTTGTCCGC TTGCCACCAGACCTCTTACTTGTTCCCTGTTTGATGCCATTTTCTCACACTCTTTCCTTATCATGCCTCA TTGCCCCACCCCCATTCTAGGCTGAGACCTTTTAGCCAAAGTCAAAGCTTCTATCACCTTTTCTTGCCCC CTTCAACCAGAGTCCCCTCCTGCTCCTCTCCGCTAGTCCGGCCCCTGACCCCTTTCCCCAGTACCCACTT CCTGCCTCTCTCATTAACCCTTCCGTAGCTGCTCACCATGACCCCATCAGAATCCAGTTAAAAGACTCTT CCAAATTTCCCAGCGTTCCTCAATACCCCATCTCTCTAACCCACCAAAAGGGCCTACAGCCCATCGTAAA CAAGCTCTGCTCATGCAGTCTTCTTAGACCAACACACTCTCCATATAACACCCCTATCCTCCCTGTTAAA AAGTCTGATGGCTCATACCGACTCGTTCACAACCTCCAAGCCATCAGTCAAGCTGTCCTCCCTATTCATC CCATAGTCCATAACCCCTATCCACTTCTCTCTCTCGTCGCTACCAACACCACCCTCTACACTGCAATTGA CCTGAAGGATGCCTTCTTTACCATTTCCCTACACCCTGATTCCCAAAACCTATTTGCTTTCACCTGGACT GACCCTGACACCCTCCAGTCACAACAACTCACATGGACTGTCCTCCCTCAGGGCTTCAGGGATAGCCCTT CTTTGGACAAGCTCTAGCCCGAAACCTCACTTCCTTAAACCTGTCTCCCAGTCATCTTCAATATGTGGAC GACCTTCTTCTTTGCAGCCCCTCTCTAAAAGACTCTCAAACTCACACGGCCACTGCTCTCTTAAACTTTC TCGCTATCAAAGGGTATAGGGTCTCCCCCTCCAAGGCCCAACTCTCCATCTCCATGATGACTTACTTAGG AATTCAACTTTCCCCTGGGGCCCAGGCTATGACTCCAGCCAGGGCAGCATTAATAGAAAATCTACCCCCA CCCTCCTCCAAAAGCGAAATCCTTTCCTTCCCAGGGCTAGCAGGCTTCTTTAGAATATGGATTTCCAACT TTGCCCTCCTAGCTCATCCCCTCTATGAAGTGGCCAAAGGCCCTCCCAATGAACCCCTAAACCCCTCACA TAACATACTCCCCAGCTTCCACAAACTCCAAACTGCTCTTGTCACTGCGTCAGCTCCGTCCTTACCTGAT ATCTCCCAACCTTTCACTCTCTATACTGCTGAAAGCTGAGGAATAGCCCTCGGTGTCTTAGGACAACAGA AAGGAAATCCTCCTTCCTTTGCCCCTGTAGCTTACCTCTCTAAACAACTAGATAACACAGTCAAAGGGTG GCCAGCCTGCTTTAAAGCACTAGAAGTGGTAGCCAGCTTAGCTCTAGAAAGCAGGAAACTAACTTTCAGC CAGAATACCACCGTCCACAGTTCTCATAATCTACAAGATCTCCTCTCCTCCCAAGCAGTAAGCTCCCTTC CTCCTTCCAGGATTCAATTACTCCATGCCCTCTTTATAAAAAATCCCAAATTCAGTCTTACCAGAAGTGC TTCCCTCAACCCAGCATCCTTACTCCCCGTATCCTCTTCCCTTCCTACTCATTCTTCCACTGATATCCTG GACCACGTACAGCCACATTTCCCAAACACTTCCTCCGAGCCTCTCACCAACCCCAATGATCAACTATTCA TAGATGGCTCCTCTTCCAGGCCCACCAGCTACCCCCCAGATTGCTGGATATGCAGTTGTTTCCCTTGACC AAGTAATTGAAGCCAGGATCCTCCTCCCAAAAAGCAGAACTCATAGCTCTCACCAGGGCCCTAAATCTTT CCAAAGGCAAACGAGTCAACATTTATACAGACTCCAAATATGCCTATCACATTCCTCGTTCCCACGCTGC TATCTGGCAAAAGAGAGGACTCCTTACTGCCAAAGGAACCCCTATCACTAATGGCCACCTTATTTACTAA CTCCTTCAGGCCACACACCTCCCAGCTAAAGCAGGAGTTATACACTGTTGAGGACATCGAACAGGTTCAG ATGAAATCTCGAGGCAACAGAAAGGCCGATGAGGCAGCAAAAGAAGCCTCCCTTTCTTCTGCCTCTGCCC CTCTCCTTCCTGTTATCCCAGCAATCCTACCCAAGAACTCTCCCACCGAGAAAGCTTTGCTATACAGCAA GGAGCCTCCTTTCAAGGGGACTGGATAGTCAAAAATCAAAAGCTCGTCCTCCCCTAAGAGCAGACCAAAG AAATTCTGACATCTCTTCACCAATCCTTCCATATCAGTGTGCGCCCCCATACCTACTCCTTCACCCTATT TCTCCTCCCCCCATCTATTCACCTCACTAAGAGACATAACCTCAAACTGTCCTATATGCTCTGTTACTTC CTCCCAAGGGGCCCTCCACTCTCCCTCCATCCCTACACATCCCCTCAGAGGAACACTCCCAGGGGAGGAC TGGCAAATAGACTTCACCCACATTCATCCCGTCAAGAGGACAAAATTTATTCTTACTCTTATAGACACCT TCTCTAGGTGGGTAGAAGCATTTCCTACCTCTTCAGAAAAGGCCGCAGAAGTCTCCCAAATTCTTGTAAC AGAAATCATCCCTACATTTGGTCTCCCTGGCTCCATACAATCAGACAATGGCCCCTAGCTTCATCTCCCA AATCACTCAACAGGTTTCTCAGTCCCTTGGCATCCAGTGGCGTCTCCATATCCCATGCTGGCCCCAGACA TCCGGAAAAGTCGAAAGGGCAAATGGGATCCTTAAGGCTCAGTTGACCAAACTCACTCTTGAAGTCCAAA AACCATGGACCTCCCTTTTGCCCATAGCACTGGAGAGCATTAGAGCCAGTCCAAAAGCACCCTCCTTCCT CAGTCCATTTGAGTTAATATACGGACGCCCTTTCCTCTTACAAAACAGGCCCCCTTCTAACTCTCAGCTA GGAGAATACCTCCCAACAGTCTCCCTCATGAGCTATCTCCTCTGCCAACAAGCCGACCAGGCCCTCCCAA AACCCCACGAAGGGGTCTCCAATCCCAAATAGACTTGCTCTCCAATTCCAAAAGACTCTTTAAGCAGCAG TGACACTTCAAAACCATCGAGGTTTAGATCTCCTCACTGCCGAGAAAGGCGGCCTGTGTATCTTTTTAGA AGAGGAGTGCTGTCTCTATACTAACCAGTCAGGACTAGTACAAGATGCTGCTGGACGAATAAATGAAAAA GCTTCTGGCCGGGTGCAGTGGCTCACGCCTGTAATCCCAGATCTTTGGGAGGCTGAGGCGGGCGGATCAC GAGGTCAGGAGATCGAGACCATCTTGGCTAACACGGTGAAACCCCGTCTCTACTAAAAATAGAAAAAATT AGCCGGGCGTGGTGGCCGGCAGCTGTACTCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATGGCGTGTGG GGTGAACCCGGGAGGCGGAGCTTGCAGTGAACAGAGATGATGCCACTGCACTCCAGCCTGGGCGACAGAG CAAGACTCCATCTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK023847 unedited AGGACTGCCGGATTTAGGTNACCACCATTCACTATNAGGGCGAAACCCGAATTCGCACCA NATTTCCTACCTCTTCAGAAAAGCCGCAGAATCTCCCAAATCTTGTAACAGAAATATCCC TACATTTGGTCTCCCTGGCTCCATACAATCAGACAATGGCCCCTAGCTTCATCTCCCAAA TCACTCAACAGGTTTCTCAGTCCCTTGGCATCCATTGTTTTCTCCATATCCCATGCTGGC CCCAGACATCCGGAAAAGTCGAAAGGGCAAATGGGATCCTTAAGGCTCAGTTAACCAAAC TCACTCTTGAAGTCCAAAAACCATGGACCTCCCTTTTGCCCATAGCACTGGAGAGCATTA GAGCCAGTCCAAAAGCACCCTCCTTCCTCAGTCCATTTGAGTTAATATACGGACGCCCTT TCCTCTTACAAAACAGGCCCCCTTCTAACTCTCAGCTAGGAGAATACCTCCCAACAGTCT CCCTCATGAGCTATCTCCTCTGCCAACAAGCCGACCAGGCCCTCCCAAAACCCCACGAAG GGGTCTCCAATCCCAAATAGACTTGCTCTCCAATTCCAAAAGACTCTTTAAGCAGCAGTG ACACTTCAAAACCATCGAGGTTTAGATCTCCTCACTGCCGAGAAAGGCGGCCTGTGTATC TTTTTAGAAGAGGAGTGCTGTTTCTATACTAACCAGTCAGGACTAGTACAAGATGCTGCT GGACGAATAAATGAAAAAGCTTCTGGCCGGTGCAGGGGGGCCCACCCCCCGGNNNCCCCG GTNGTTGTGGGCGTTCCCCGCGCCGGTTCCCCGGGCGCNCGGTTCCCGCCTCTGCTCGTC CCTCCCCCCGTNGCCCCCCTCTTCCCTCTTNNTTTCCTNCTCTTCCCGCGCCTCTTTACT GAAAATAATAAAA
Restriction Sites	NotI-NotI
ACCN	AK023847
Insert Size	1000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK023847.1
RefSeq Size	5124 bp
RefSeq ORF	5124 bp
Locus ID	221937
Gene Summary	Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis, muscle cell differentiation and autophagy (By similarity). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:17670796). Together with FOXK2, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (By similarity). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (By similarity). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (By similarity). Involved in mTORC1-mediated metabolic reprogramming: in response to mTORC1 signaling, translocates into the nucleus and regulates the expression of genes associated with glycolysis and downstream anabolic pathways, such as HIF1A, thereby regulating glucose metabolism (By similarity). Together with FOXK2, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (By similarity). Acts as a transcriptional regulator of the myogenic progenitor cell population in skeletal muscle (By similarity). Binds to the upstream enhancer region (CCAC box) of myoglobin (MB) gene, regulating the myogenic progenitor cell population (By similarity). Promotes muscle progenitor cell proliferation by repressing the transcriptional activity of FOXO4, thereby inhibiting myogenic differentiation (By similarity). Involved in remodeling processes of adult muscles that occur in response to physiological stimuli (By similarity). Required to correct temporal orchestration of molecular and cellular events necessary for muscle repair (By similarity). Represses myogenic differentiation by inhibiting MEFC activity (By similarity). Positively regulates Wnt/beta-catenin signaling by translocating DVL into the nucleus (PubMed:25805136). Reduces virus replication, probably by binding the interferon stimulated response element (ISRE) to promote antiviral gene expression (PubMed:25852164).[UniProtKB/Swiss-Prot Function]

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