FGD2 (AK092732) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZFYVE4
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092732, the custom clone sequence may differ by one or more nucleotides AGAACAGATTCATGGGTGATTTAGCCTATCTGTCCCAGGCCAGCGTGGCTGAGTGTGCTGGCTGGAGGCC TCTCTCTCTGCTTCGAGGGTAGCTGAGATCCACCCCGGAAACCGGCAGGATGAAGGGGGCAAGTGAGGAG AAGCTGGCATCTGTGTCCAACCTGGTCACTGTGTTTGAGAATAGCAGGACCCCAGAAGCAGCACCCAGAG GCCAGAGGCTAGAGGACGTGCATCACCGCCCTGAGTGCAGGCCTCCCGAGTCCCCAGGACCACGGGAGAA GACGAATGTCGGGGAGGCCGTGGGGTCTGAGCCCAGGACAGTCAGCAGGAGGTACCTGAACTCCCTGAAG AACAAGCTGTCCAGCGAAGCCTGGAGGAAATCTTGCCAGCCTGTGACCCTCTCAGGATCGGGGACGCAGG AGCCAGAGAAGAAGATCGTCCAGGAGCTGCTGGAGACAGAGCAGGCCTATGTGGCGCGCCTCCACCTGCT AGACCAGGTGTTTTTCCAGGAGCTGCTGAAGACAGCCCGCAGCAGCAAGGCCTTCCCAGAGGATGTGGTC AGGGTCATCTTCTCCAACATCTCCTCCATCTATCAGTTCCATTCTCAGTTCTTCCTCCCAGAGCTGCAGC GGCGCCTGGACGACTGGACAGCTAACCCCCGCATCGGTGACGTGATCCAGAAGCTGGCCCCCTTCCTGAA GATGTACAGTGAGTATGTCAAGAACTTTGAGCGAGCGGCTGAGCTGCTGGCCACCTGGACCGACAAGTCT CCACTCTTCCAGGAGGTTCTCACTCGCATCCAGGTGAGGCTGGGGGAGGGCTGGAGTCAGCATTGCCACT CCCAGCATGCAGTGGCTCAGGTTGCCTTGAGTGATTCCGGGCATCTCCCAGGCTCAGCTGCTTCCATAGG CCCCTGCCTACTCGTCCGGCCCTCAGGAGCAGCCTGACCCACCTCCCTTCTCTCACCCTCTCCGTGTTGC TCCCCCATCCCTCCCCAAGAGCAGCGAGGCTTCGGGCAGCCTGACCCTGCAGCACCACATGCTGGAACCA GTGCAGAGAATTCCACGTTACGAGCTGCTGCTCAAGGAGTACATCCAGAAGCTGCCAGCCCAGGCCCCAG ACCAGGCCGATGCCCAGAGACCTCCTGCCCCAGCCACCAGCCCTAGATTCTCAGGGAATAAACTCCGGCC CTGCCCCTTTCAGAAGCCCTGGACATGATCTTCTCAGCTGCCCAGCACTCCAATGCAGCCATCACTGAGA TGGAGCGGCTGCAGGACCTGTGGGAGGTGTACCAGCGCCTGGGCCTCGAGGACGACATAGTAGACCCCTC TAACACCCTGCTCCGTGAGGGCCCGGTCCTCAAGATCTCCTTCCGCCGCAACGACCCCATGGAGCGCTAC CTTTTCTTGTTCAACAACATGCTGCTCTACTGTGTGCCCAGGGTGATCCAGGTGGGCGCCCAGTTCCAGG TGAGGACCCGCATCGATGTGGCCGGGATGAAGGTGCGGGAGCTGATGGATGCTGAGTTTCCCCACTCCTT CCTGGTGTCTGGGAAGCAGCGCACCCTGGAGCTGCAAGCCCGGTCCCAGGAGGAAATGATTTCCTGGATG CAGGCCTTCCAAGCAGCCATTGACCAAATCGAGAAGCGGAATGAAACCTTCAAGGCTGCGGCCCAGGGGC CTGAGGGAGACATCCAGGAGCAGGAGCTGCAGTCTGAGGAGCTGGGCCTCCGGGCACCGCAGTGGGTCCG GGACAAGATGGTGACCATGTGCATGCGCTGCCAGGAGCCCTTCAACGCTCTGACGCGCCGTCGCCACCAC TGCCGGGCCTGCGGCTATGTGGTGTGTGCCAGGTGCTCCGACTACCGGGCCGAACTGAAATACGACGACA ACAGGCCCAACCGAGTCTGCCTCCACTGCTACGCATTCCTCACTGGAAATGTGCTGCCTGAGGCCAAGGA GGACAAGAGGCGGGGCATCCTGGAGAAAGGGTCCTCAGCCACGCCTGACCAGAGCCTGATGTGCAACTTC CTGCAGCTCATCGGGGACAAGTGGGGCAAGAGCGGCCCCCGGGGCTGGTGTGTGATCCCTCGGGATGACC CCCTCGTGCTCTATGTCTATGCTGCCCCTCAGGACATGAGGGCTCACACCTCCATCCCCCTGCTGGGCTA CCAGGTGACTGTTGGGCCCCAGGGGGACCCTCGGGTCTTCCAGCTACAGCAGTCAGGCCAGCTCTACACC TTCAAGGCCGAGACGGAGGAGCTGAAGGGCCGCTGGGTGAAGGCCATGGAGCGGGCGGCCAGTGGCTGGA GCCCCAGCTGGCCCAACGATGGGGACCTGTCCGACTGAGCCACTGCCAGCCGCTCTCCTGCCCACCTCTC CCCACCCTGAACCCAGCTCCTGCCACAGACTGACCCTGTGGCCTCAGTGACCCACTGCCCCAAGTGGTGC TTTCAGAGAATTGATTCAGCCATCTGCGCCCAGGCCACGTGTCCCGATCTGGGATTAGAAAATATGGGTC CATTCCTTTCTAGAAAGGGGACAACCAAGTGTCTCAGTTTGCCTTGCGGGGAGGGGGCTCCTGGGCCATG GGACTTCCAGTGCTAAAACTGGGAAAGCCCCAGGTAACCCCGGACTGGTGGTCACCATAGTATGGTTTTT CATTTGTATCTCCTGGGGAGCTTTTAAAGAGTACTGGTGAAAAACACATAGTAAATTAATTTT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092732 unedited GCTCCTATTTGTAAACGACTTACTATAGGCGGCCGCGCANAATGCCGTGGAAGTTTGCTT TGGGGGATTCAGGGAGCCTGGCCTCACCTGGCCCTATCTAGAGTCGAGAGGAGGTCTCTA GGCTGAGGGCAATCCCTGTGCCCCTCCTGCAGGACAGCTAACCCCCGCATCGGTGACGTG ATCCAGAAGCTGGCCCCCTTCCTGAAGATGTACAGTGAGTATGTCAAGAACTTTGAGCGA GCGGCTGAGCTGCTGGCCACCTGGACCGACAAGTCTCCACTCTTCCAGGAGGTTCTCACT CGCATCCAGCGAGGCTTCGGGCAGCCTGACCCTGCAGCACCACATGCTGGAACCAGTGCA GAGAATTCCACGTTACGAGCTGCTGCTCAAGGAGTACATCCAGAAGCTGCCAGCCCAGGC CCCAGACCAGGCCGATGCCCAGAAAGCCCTGGACATGATCTTCTCAGCTGCCCAGCACTC CAATGCAGCCATCACTGAGATGGAGCGGCTGCAGGACCTGTGGGAGGTGTACCAGCGCCT GGGCCTCGAGGACGACATAGTAGACCCCTCTAACACCCTGCTCCGTGAGGGCCCGGTCCT CAAGATCTCCTTCCGCCGCAACGACCCCATGGAGCGCTACCTTTTCTTNGTCACAACATG CTGCTCTACTGTGTGCCCANGGTGATCCAGGTGGGCGCCCAGTTCCAGGTGAGGACCCGC ATCGATGTGGCCCGGNATGAAAGTGCGGGAGCTGATGGATGCTGAGTTTTCCCACTCCTT CCTGGTGTCCGGGAAGCAGCGCACCCTGGAGCTGCAAGCCCCGTTCCCAGGAGAAATGAT TTCCTGGGTTGCAGCCCTTCAAGCAGCCCTTTGACAAATCGAGNAAGCGGATGAAAACTT CAGGCTGCGGCCCAGGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK092732 unedited GACGCTAACTGTGTTGTTTCCCTCATCCTANGTTCNGTTTTATTTTTTTTTTTTTTTCAC AAAGCCTGTCCCAATAAATGTTTATTATTATTATTACTACTATCATGACTGGCACTTGAC AGGGGCTCAACCATTGGTTGTGGTAGTTTGCTCTCTTTATTCTTCATCCCAGGTAACCCA GGCTATATATTAGACTCCAGTGGGTACTCTTAAAAAATATGCATGCGCCAGGTATGGTGG CTCACGCCTGTAATCCCAGCACTTTGGGAGGTGGAGGTGGGAGCATCGCTTGAGGCTGGG AGTTTGAGACCAGCCTGAGTAACATGGTGAAATCCCATCTCTACAAAAAATAAAAAAATT AGCTTGGCACAGTGGCACACACCTATAATCCCAGCTACTTGGGAGGCTGAGGCGGGAGGA TCACTGGAGCCCGGGAGAGCGAGGCTGTAGTGAGCCATGTTTGTGCACTCCAGCCTGGGA AACAGAGTAAGACTCTGTCTCAAAAAAAAAAAAAAAAGACCCCATGCCTCCCCCAACTCA GCCCATTTAAATCGGTGGTGGTACTTCAGTACAGGCATTGTTTTTTACATTTTTAAAATT AATTTACTATGTGTTTTTCACCAGTACTCTTTAAAAGCTCCCCAGGAGATACAAATGAAA AACCATACTATGGTGACCACCAGTCCGGGGTTACCTGGGGCTTTCCCAGTTTTAGCACTG GAAGTCCCATGGCCCATGAGCCCCCTCCCCGCAAGGCAAACTGAGACACTTGGTTTGTCC CCTTTCTAGAAAGGAATGGACCCATATTTTCTAACCCCAGATCGGGACACGTGGCCTGGG CGCANATGGCTGAATCATTCC
Restriction Sites	NotI-NotI
ACCN	AK092732
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK092732.1
RefSeq Size	2723 bp
RefSeq ORF	2723 bp
Locus ID	221472
Domains	FYVE, PH
Gene Summary	The protein encoded by this gene belongs to a family of guanine nucleotide exchange factors (GEFs) which control cytoskeleton-dependent membrane rearrangements by activating the cell division cycle 42 (CDC42) protein. This gene is expressed in B lymphocytes, macrophages, and dendritic cells. The encoded protein may play a role in leukocyte signaling and vesicle trafficking in antigen-presenting cells in the immune system. [provided by RefSeq, Oct 2016]

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