MTAP (AF109294) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BDMF; c86fus; DMSFH; DMSMFH; HEL-249; LGMBF; MSAP
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF109294, the custom clone sequence may differ by one or more nucleotides ATGGCCTCTGGCACCACCACCACCGCCGTGAAGATTGGAATAATTGGTGGAACAGGCCTGGATGATCCAG AAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCAAGCCATCTGATGCCTTAATTTT GGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAAGGCATGGAAGGCAGCACACCATCATGCCTTCA AAGGTCAACTACCAGGCGAACATCTGGGCTTTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAG CTTGTGGCTCCTTGAGGGAGGAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGAC TATTTGCCACGACATTTCAAAGGATTCCAAGAGAGAATATTGGTGTCCATGCTGTGATGATTCCTCAGCT CCTCTCATCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF109294 unedited NNCCGTTCGAATTTTGTATACGTACTTCACTATAGGGCGGCCGCGAATTCGCACCAGNAC CACCGCTCTGTGGCTCGCTTGGTTCCCTTAGTCCCGAGCGCTCGCCCACTGCAGATTCCT TTCCCGTGCAGACATGGCCTCTGGCACCACCACCACCGCCGTGAAGGTGAGATGAGCCCT CCCAGCCGCAGCGGTTCGCCCTGCCGGATGCCTTCTCGCCCCCGCGCCGGGGGACCGCGC CTCCGGGGGCCATGCGCCCGGCCCGTGCGTCCCTTGCCGCCGCGGGGAGGGACTGGGGCG CGGCACTCGGGACTCACTTGCCGCGCGAGGAGAGGATTGGAATAATTGGTGGAACAGGCC TGGATGATCCAGAAATTTTAGAAGGAAGAACTGAAAAATATGTGGATACTCCATTTGGCA AGCCATCTGATGCCTTAATTTTGGGGAAGATAAAAAATGTTGATTGCATCCTCCTTGCAA GGCATGGAAGGCAGCACACCATCATGCCTTCAAAGGTCAACTACCAGGCGAACATCTGGG CTTTGAAGGAAGAGGGCTGTACACATGTCATAGTGACCACAGCTTGTGGCTCCTTGAGGG AGGAGATTCAGCCCGGCGATATTGTCATTATTGATCAGTTCATTGACAGGACCACTATGA GACCTCAGTCCTTCTATGATGGAAGTCATTCTTGTGCCAGAGGAGTGTGCCATATTCCAA TGGCTGAGCCGTTTTGCCCCAAAACGAGAGAGGTTCTTATAGAGACTGCTAAGAAGCTAG GACTCCGGTGCCACTCAAAGGGGACAATGGTCACCATCGAGGGACCTCGGTTTTAGCTCC CGGGCAGAAAGCTTCATGTTTCGCACCCTGGGGGCGGGATGTTATCAACTGGACCCCATT CCAGG
Restriction Sites	NotI-NotI
ACCN	AF109294
Insert Size	2000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF109294.1, AAD19641.1
RefSeq Size	942 bp
RefSeq ORF	942 bp
Locus ID	4507
Domains	Mtap_PNP
Protein Families	Druggable Genome
Protein Pathways	Cysteine and methionine metabolism, Metabolic pathways
Gene Summary	This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008]

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