C1orf186 (BD078463) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD078463, the custom clone sequence may differ by one or more nucleotides GAAGGGTCGGGGAGATATTTCCGTTAGACATCGCTGAAACACAGACTGGGATCAAACTGTGCTCATAGTC CTAAGGATCTCCAGCACCCTGCCGGTGGCACTACTGAGAGACGAGGTGCCAGGGTGGTTCCTGAAARTGC CTGAGCCCCAACTTATCAGCAAGGAGCTCATCATGCTGACAGAAGTCATGGAGGTCTGGCATGGCTTAGT GATCGCGGTGGTGTCCCTCTTCCTGCAGGCCTGCTTCCTCACCGCCATCAACTACCTGCTCAGCAGGCAC ATGGGTAACTGGCTCAGCATCCTCTTCCCTCCTAGTCACTCTCAGAGACCATTCTCGAGCCTCCAGCAGG ACAGACCCTTTGGAGTTCCCAAACGTCACTCAAAAACTACCAGAGGACCCACCGGCCAAATTCCTTCCCA CCGCTCCCCCTCCCCCCAATAACTGTATCTGGGTAATCCCCACTCTGACCTCACCTTTTAACCAACTATT TCTGGCTGGAAGTGGCCATCCACATCCGTCTACTACCCAGACCTTCTGCCTAGACACAGCTTTTGCAATG TCCTACGAGGAAGTGCTCGTGTAACCTGGTCTAATTAATTTTCTTCATCCCTGTTAAAGGACTGAATATG AAGAAATGTCCTTGAATTACAACAGAAGGAAATATGGTTGGACTTAGAGATTAGTTTAAATTCTTGAACT GATAAACAATAGAAGGTAGTGAAGCTCGGTCCTGGAAAGGCATTTCAATTAGGGAAAATAAAACAATGCT GCTTTGGTTGTGCTAAGAAAAAAAAAAAAAAAAAAAAACTCGTAGGGGGGGTCTTGGTACCCAATNGTCC T 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD078463 unedited TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCGAGNNAGTAGCTGAGCTTTGG CCCAAGAGGAAATTTGTATAAAGAGCCCTGGAGTCCTGACCAGCTCCTTACCTCTTGCAG ATCTCCAGCACCCTGCCGGTGGCACTACTGAGAGACGAGGTGCCAGGGTGGTTCCTGAAA GTGCCTGAGCCCCAACTTATCAGCAAGGAGCTCATCATGCTGACAGAAGTCATGGAGGTC TGGCATGGCTTAGTGATCGCGGTGGTGTCCCTCTTCCTGCAGGCCTGCTTCCTCACCGCC ATCAACTACCTGCTCAGCAGGCACATGGGTAACTGGCTCAGCATCCTCTTCCCTCCTAGT CACTCTCAGAGACCATTCTCGAGCCTCCAGCAGGACAAGACCCCTTTGGAGTTCCCAAAC GTCACTCAAAAACCTACCAGAGGACCCACCGGCCAAATTCCTTCCCACCGCTCCCCCTCC CCCCAATAACTGTATCTGGGTAATCCCCACTCTGACCTCACCTTTTAACCAACTATTTCT GGCTGGAAGTGGCCATCCACATCCGTCTACTACCCAGACCTTCTGCCTAGACACAGCTTT TGCAATGCCTACGAGGAAGTGCTCGTGTAACCTGGTCTAATTAATTTTCTTCATCCCTGT TAAAGGACTGAATATGAAGAAATGTCCTTGAATTACAACAGAAGGAAATATGGTTGGACT TTAGAGATAGTTAANATTCTTGAACTGATAAACAATAGAAGGTAGTGAAGCTAGGTCCTG GAAAGGCATTTCACNTAGGGAAATAAACATGCTGCTTTGGTGTGCTAAAGAAAGACNNGG CAGAGATGTTTGAGGTCTTCTAGTAANCACCTTATCATCATTGTCATCATCATCAGAGAA TTCTGACATCTAGACTCTCTGGCTAGCTCCACAGGACAA
Restriction Sites	NotI-NotI
ACCN	BD078463
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BD078463.1
RefSeq Size	841 bp
RefSeq ORF	841 bp
Locus ID	440712
Protein Families	Transmembrane
Gene Summary	Acts as a signaling transduction factor of the EPO-EPOR signaling pathway promoting erythroid cell differentiation (PubMed:25092874).[UniProtKB/Swiss-Prot Function]

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