FADS1 (BD127299) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD127299, the custom clone sequence may differ by one or more nucleotides GTGAGTTGCCCAAGACCCACCGGGGGACGGGATCTCGCTCCCCGCGCCACGAGGCTCGGCCAATGGGAAC GCGCGCTGCGAGGCCCGCCGGTCTGCCCTGCGGTGCTGAAAACCCGGCGCGCAGGCGGCTGGCTCTGGGC GCGCGCCAGCAAATCCACTCCTGGAGCCCGCGGACCCCGAGCACGCGCCTGACAGCCCCTGCTGGCCCGG CGCGCGGCGTCGCCAGGCCAGCTATGGCCCCCGACCCGGTGGCCGCCGAGACCGCGGCTCAGGGACCTAC CCCGCGCTACTTCACCTGGGACGAGGTGGCCCAGCGCTCAGGGTGCGAGGAGCGGTGGCTAGTGATCGAC CGTAAGGTGTACAACATCAGCGAGTTCACCCGCCGGCATCCAGGGGGCTCCCGGGTCATCAGCCACTACG CCGGGCAGGATGCCACGGATCCCTTTGTGGCCTTCCACATCAACAAGGGCCTTGTGAAGAAGTATATGAA CTCTCTCCTGATTGGAGAACTGTCTCCAGAGCAGCCCAGCTTTGAGCCCACCAAGAATAAAGAGCTGACA GATGAGTTCCGGGAGCTGCGGGCCACAGTGGAGCGGATGGGGCTCATGAAGGCCAACCATGTCTTCTTCC TGCTGTACCTGCTGCACATCTTGCTGCTGGATGGTGCAGCCTGGCTCACCCTTTGGGTCTTTGGGACGTC CTTTTTGCCCTTCCTCCTCTGTGCGGTGCTGCTCAGTGCAGTTCAGGCCCAGGCTGGCTGGCTGCAGCAT GACTTTGGGCACCTGTCGGTCTTCAGCACCTCAAAGTGGAACCATCTGCTACATCATTTTGTGATTGGCC ACCTGAAGGGGGCCCCCGCCAGTTGGTGGAACCACATGCACTTCCAGCACCATGCCAAGCCCAACTGCTT CCGCAAAGACCCAGACATCAACATGCATCCCTTCTTCTTTGCCTTGGGGAAGATCCTCTCTGTGGAGCTT GGGAAACAGAAGAAAAAATATATGCCGTACAACCACCAGCACAAATACTTCTTCCTAATTGGGCCCCCAG CCTTGCTGCCTCTCTACTTCCAGTGGTATATTTTCTATTTTGTTATCCAGCGAAAGAAGTGGGTGGACTT GGCCTGGATGATTACCTTCTACGTCCGCTTCTTCCTCACTTATGTGCCACTATTGGGGCTGAAAGCCTTC CTGGGCCTTTTCTTCATAGTCAGGTTCCTGGAAAGCAACTGGTTTGTGTGGGTGACACAGATGAACCATA TTCCCATGCACATTGATCATGACCGGAACATGGACTGGGTTTCCACCCAGCTCCAGGCCACATGCAATGT CCACAAGTCTGCCTTCAATGACTGGTTCAGTGGACACCTCAACTCCCAGATTGAGCACCATCTTTTTCCC ACGATGCCTCGACACAATTACCACAAAGTGGCTCCCCTGGTGCAGTCCTTGTGTGCCAAGCATGGCATAG AGTACCAGTCCAAGCCCCTGCTGTCAGCCTTCGCCGACATCATCCACTCACTAGAGGAGTCAGGGCAGCT CTGGCTAGATGCCTATCTTCACCAATAACAACAGCCACCCTGCCCAGTCTGGAAGAAGAGGAGGAAGACT CTGGAGCCAAGGCAGAGGGGAGCTTGAGGGACAATGCCACTATAGTTTAATACTCAGAGGGGGTTGGGTT TGGGGACATAAAGCCTCTGACTCAAACTCCTCCCTTTTATCTTCTAGCCACAGTTCTAAGACCCAAAGTG GGGGGTGGACACAGAAGTCCCTAGGAGGGAAGGAGCTGTTGGGGCAGGGGTGTAAATTATTTCCTTTTTC TAGTTTGGCACATGCAGGTAGTTGGTGAACAGAGAGAACCAGGAGGGTAACAGAAGAGGAGGGACCTACT GAACCCAGAGTCAGGAAGAGATTTAACACTAAAATTCCACTCATGCCGGGCGTGGTGGCACGCGCCTGTA ATCCCAGCTACCCAGGAGGCTGAGGCAGGAGAATCGCTTGAACCGGGGAGGTGGAGGTTGCAGTGAGCTG AGATCACGCCATTGTACTCCAGCCTGGGCGACAGAGCAAGACTCCATTTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD127299 unedited TCTATAACCCGCCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA AGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCG CGAATTCGGCACGAGGGTTGCCCAAGACCCACCGGGGGACGGGATCTCGCTCCCCGCGCC ACGAGGCTCGGCCAATGGGAACGCGCGCTGCGAGGCCCGCCGGTCTGCCCTGCGGTGCTG AAAACCCGGCGCGCAGGCGGCTGGCTCTGGGCGCGCGCCAGCAAATCCACTCCTGGAGCC CGCGGACCCCGAGCACGCGCCTGACAGCCCCTGCTGGCCCGGCGCGCGGCGTCGCCAGGC CAGCTATGGCCCCCGACCCGGTGGCCGCCGAGACCGCGGCTCAGGGACCTACCCCGCGCT ACTTCACCTGGGACGAGGTGGCCCAGCGCTCAGGGTGCGAGGAGCGGTGGCTAGTGATCG ACCGTAAGGTGTACAACATCAGCGAGTTCACCCGCCGGCATCCAGGGGGCTCCCGGGTCA TCAGCCACTACGCCGGGCAGGATGCCACGGATCCCTTTGTGGCCTTCCACATCAACAAGG GCCTTGTGAAGAAGTATATGAACTCTCTCCTGATTGGAGAACTGTCTCCAGAGCAGCCCA GCTTTGAGCCCACCAAGAATAAAGAGCTGACAGATGAGTTCCGGGAGCTGCGGGCCACAG TGGAGCGGATGGGGCTCATGAAGGCCAACCATGTCTTCTTCCTGCTGTACCTGCTGCACA TCTTGCTGCTGGATGGTGCAGCCTGGCTCACCCTTTGGGTCTTTGGGACGTCCTTTTTGC CCTTCCTCCTCTGTGCCGTGCTGCTCAGTGCAGTTCAGGCCCCAGCTGGCTGGCTGCAGC ATGACTTTGGGCACCCTGTCGTCTTCAGCACTCAAGTGGAACC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BD127299 unedited TATGGCCGCGGCCGCATCTAAATCGAGTTTTTTTTTTTTTTTTTTGAAATGGAGTCTTGC TCTGTCGCCCAGGCTGGAGTACAATGGCGTGATCTCAGCTCACTGCAACCTCCACCTCCC CGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGGGTAGCTGGGATTACAGGCGCGTGCCA CCACGCCCGGCATGAGTGGAATTTTAGTGTTAAATCTCTTCCTGACTCTGGGTTCAGTAG GTCCCTCCTCTTCTGTTACCCTCCTGGTTCTCTCTGTTCACCAACTACCTGCATGTGCCA AACTAGAAAAAGGAAATAATTTACACCCCTGCCCCAACAGCTCCTTCCCTCCTAGGGACT TCTGTGTCCACCCCCCACTTTGGGTCTTAAAACTGTGGCTAGAAGATAAAAGGGAGGAGT CCGAGTCAGAGGCTTTATGTCCCCAAACCCAACCCCCTCTGAGTATTAAACTATAGTGGC ATTGTCCCTCAAGCTCCCCTCTGCCTTGGCTCCAGAGTCTTCCTCCTCTTCTTCCAGACT GGGCACGGTGGCTGCCGTTATTGGCGAAGATAGGCCTCTAGCCCGAGCCGCCCTGACTCC TTTACGAGTGGATGATGTCCGCGAAGGTTGACAGCAGGGGCTCGGACTGGTACTCTATGC CATGCTTTGCCACACAAGGACTGCCCCAGGGGCACCCACCTTTGGGGCCATTCGCCGTCG AGGCCATCGTCGGCACAAAGACGGGCGCCCCAACCCTCCCACCTCGCAGGTGCCCACCCT GAACCCGCTCCTTTGAACGCCCGCCCTTGGCGGACCTCCCCCAGTGGGTCTTGGAGCCCG GGCCGGAAACCCCAGTTCATGNTCCNNNCCCCCAACAAANGNNCNNNCNAACCNNNNNAC NNCNNNNNNNNCNNNNNNNTNNTNCNNNNTNNNNNNNNANCCNNCCTCCNNNNNCNNNNC NCNCCCNNCNCCCCCNCCCCCCCCCCCCCCCCCCNNCNNNCNNCNNNNCNNCCNNNNNNN NNNNCCCCCCCCCCCTGTCCCNCNG
Restriction Sites	NotI-NotI
ACCN	BD127299
Insert Size	2130 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BD127299.1
RefSeq Size	2080 bp
RefSeq ORF	2080 bp
Locus ID	3992
Domains	heme_1, FA_desaturase
Protein Families	Transcription Factors, Transmembrane
Protein Pathways	Biosynthesis of unsaturated fatty acids
Gene Summary	The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]

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