HECTD3 (AK096462) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK096462, the custom clone sequence may differ by one or more nucleotides ATGAAGAAGGGCACCATTGTCAAGAAGCTGCTACTCACAGTGGATACCACAGATGACAACTTTATGCCAA AGCGGGTGGTGGTCTATGGGGGTGAAGGGGACAACCTGAAGAAGCTGAGTGACGTGAGCATTGACGAGAC CCTCATCGGGGATGTCTGTGTCCTGGAGGACATGACCGTCCACCTCCCGATCATCGAGATCCGCATCGTG GAGTGCCGAGATGATGGGATTGATGTTCGTCTCCGAGGGGTCAAGATCAAGTCATCTAGACAGCGGGAAC TAGGGTTGAATGCAGACCTGTTCCAGCCAACTAGTCTGGTGCGATATCCACGCCTAGAAGGCACCGACCC TGAAGTACTGTACCGCAGAGCTGTCCTCCTGCAGAGATTCATCAAGATCCTCGATAGTGTCCTGCACCAC CTGGTACCTGCCTGGGACCACACACTGGGCACCTTCAGTGAGATTAAGCAAGTGAAGCAGTTCCTACTGC TGTCCCGCCAGCGGCCAGGCCTGGTGGCTCAGTGCCTGCGTGACTCTGAGAGCAGCAAGCCCAGCTTCAT GCCACGCCTATACATCAACCGCCGTCTTGCCATGGAACACCGTGCCTGCCCCTCTCGAGACCCTGCCTGC AAGAATGCAGTCTTCACCCAGGTATATGAAGGCCTCAAGCCCTCTGACAAATATGAAAAGCCCCTGGACT ACAGGTGGCCCATGCGCTATGACCAGTGGTGGGAGTGTAAATTTATTGCAGAAGGCATCATTGACCAAGG GGGTGGTTTCCGGGACAGCCTGGCAGATATGTCAGAAGAGCTGTGCCCTAGCTCAGCGGATACCCCCGTG CCCCTGCCCTTCTTTGTACGCACAGCCAACCAGGGCAATGGCACTGGTGAGGCTCGGGACATGTATGTAC CCAACCCCTCCTGCCGAGACTTTGCCAAGTATGAATGGATCGGACAGCTGATGGGGGCTGCCCTTCGGGG TAAGGAGTTCCTGGTCCTGGCCCTGCCTGGTTTTGTGTGGAAGCAGCTTTCTGGTGAGGAGGTGAGCTGG AGCTGGTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK096462 unedited TCATAGGCGGCCGCGACTTCGGCACGAGGCAAAGGCGGGGAAGGGCGTGGTGGGAAGCTC TGTCCGCGGTCGCGCCAGCTGTTCCCCGGGCAGGGTCGCCTCTAGGTGCTCACCTCCGCC ACTTCGCCATGGCGGGTCCTGGCCCGGGCGCGGTGCTGGAGTCCCCCCGGCAGCTGCTGG GCCGCGTGCGCTTCTTGGCAGAGGCAGCGCGGAGCCTCCGCGCCGGGCGGCCGCTGCCAG CAGCGCTGGCTTTCGTGCCGCGAGAGGTGCTCTACAAGCTTTACAAGGACCCAGCGGGAC CGTCGCGCGTGCTTCTGCCGGTGTGGGAGGCAGAGGGCCTGGGCTGCNGTGGGGGCGCCC GCAGGCCCAGCCCCCGGTACCGGCTCCGGGCCCCTTCGCGCCGCCCGCGACAGCATTGAG CTCCGGCGCGGCGCCTGCGTGCGCACCACGGGCGAGGAGCTGTGCAATGGCCACGGGCTC TGGGTGAAGCTGACAAAGGAGCAGCTGGCAGAGCACCTGGGCGACTGCGGGCTGCAGGAA GGCTGGCTGCTGGTGTGCCGCCCGGCGGAGGGCGGAGCCCGCCTGGTACCCATCGACACT CCCAACCACCTCCAGCGGCAGCAGCAGCTCTTTGGCGTGGATTATCGGCCGGTGCTCAGG TGGGAACAGGGTGGGGGACCTGACATACTCACATCGCCTGGGATCGAGACCTCAACCGGC AGAAGCATACGCAGAAGCTGGACAAAGGCTACTCTATGTACCCCCGACATGGACCTACGA GTGCGACGAGGACCTGATCCACTTTTTGGATGAACACCCTGGCAAGGGAAGATGAGAACC CTGGGTAGCGGGAAGCATTATGTGGGAAGAATTAAACGTTTCCTCCTACCGGGAGGAATT AAACGTGTCCTGCCTGAAGACAGCATGGCCAAAC
Restriction Sites	NotI-NotI
ACCN	AK096462
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK096462.1, BAG53296.1
RefSeq Size	3506 bp
RefSeq ORF	1059 bp
Locus ID	79654
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8. [provided by RefSeq, Dec 2012]

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