SCD5 (AL831891) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ACOD4; FADS4; HSCD5; SCD2; SCD4
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL831891, the custom clone sequence may differ by one or more nucleotides GGGGAGAAAGCTTGACGTCACTGACCTGCTTGCTGATCCTGTGGTCCGGATCCAGAGAAAGTACTATAAG ATCTCCGTGGTGCTCATGTGCTTTGTGGTCCCCACGCTGGTGCCCTGGTACATCTGGGGAGAGAGTCTGT GGAATTCCTACTTCTTGGCCTCTATTCTCCGCTATACCATCTCACTCAACATCAGCTGGCTGGTCAACAG CGCCGCCCACATGTATGGAAACCGGCCCTATGACAAGCACATCAGCCCTCGGCAGAACCCACTCGTCGCT CTGGGTGCCATTGGTGAAGGCTTCCATAATTACCATCACACCTTTCCCTTTGACTACTCTGCGAGTGAAT TTGGCTTAAATTTTAACCCAACCACCTGGTTCATTGATTTCATGTGCTGGCTGGGGCTGGCCACTGACCG CAAACGGGCAACCAAGCCGATGATCGAGGCCCGGAAGGCCAGGACTGGAGACAGCAGTGCTTGAACTTGG AACAGCCATCCCACATGTCTGCCGTTGCAACCTCGGTTCATGGCTTTGGTTACAATAGCTCTCTTGTACA TTGGATCGTGGGAGGGGGCAGAGGGTGGGGAAGGAACGAATCAATGTGGTTTGGGAATGTTTTTGTTTAT CTCAAAATAATGTTGAAATACAATTATCAATGAAAAAACTTTTGTTTTTTTTTTTTGTTTGTTTTGTTTT TGAGACAGAGTCTCACTCTGTCACCCAGGCTGGAGTGCAGTGGCGCAGTCTCGGCTCACTGCAGCCTCCA CCTACCTGGTTCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGAGATTACAGGAGCCTGCCACCAC ACCCAGCTAATTTTTTTGTATTTTTAGTAGAGACAGGGTTTCATCATGTTGGCCAGACTGGCCTCGAATT CCTGACCTCAGGCAATCCACCCGCCTCGGCCTCCCAAAGAGCTGGGATTACAGGCGTGAGCCACCGCACC CTGCCGAAAAAAACTTTTTTTTTTTGAGACGGAGGCTCGCTCTGTCCCCCAGGCTGGAGTGCAGTGGCGA GATTTCAGCTCACTGCAAGCTCCGCCTCCCGGGTTCAGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTG GGAGCCAGCGCGCCCAGCCTAAAAAACTTTTCAAGTCAATATTACTACGATTTAACATTAGAGTGTGGAC ATGTGATTTAATCGCTATAGCTAAAATACGTCAAATATACGTTGTCATGTGCTTGAACATGATGCTAACC CTGACAGGATGAAGGAAAGTAATATTCTTTCAGTGTAGTTCAGGAGAGCATTTGTTTTCTTTTCTACCAA TTAACCCATCATTGCTTTTAAACAACCATCTGAAGGAGCAGAGAGGCAGGGTAGAAGACAGAAGGGGGAT CTATGTGGTAACTAAAGAATGTTTCTGTTTTGTTAATTATTGTGTGTGTGTGGTTTTATTGTTTGCTTAA GAGAATCAAAAACTGAAAAAAATGAGAATACAGGAAATGGCTCTTGTTTATTTTTTTGCTGTGTTTACAG CTTGTTAATGCTCTACTGTCTTTGTTTCAAGAGAGATTTGTTCACTGCCCAGCTCGTTTTGTGTCCTGAG CCCTATGGCCAGCCCACCTTATAAATCATGCCTGTTTAGATGTTTGATTTTGTTCTGTTTGCTATTGTTA TCTTAAAGGTGTATAACTCTGACATGCCAGACATCAAATTAAGCTCAAATTAAGCTCTCGTTTAAATGTT TAAGCACCTAATTTATATTCTAATTGATCCCAGCCACTGATGCATGTACTTTAGCTACTTCTGCTAAATA AGCATATTAATTTTCCACATCAGACCATCAGATCTTGAGAACCAACAGTTATCTAGAATTCCGTGTCTAC TAATGTTTCACCTGCATGCAGCCTTCATTAATTTTGTAGCAAAATATAAAGTGATCATTATGTAGCTTCT GGATTAAAAAAATTTGTGTGTGAAGTTGCTTTGTAAAGTGCATGTGGAATTAATGGGACAGTGTGCCCTT TGTGTTAGATGTTAGAGCAAAAGAAAGGGCTTATAGTGTTAGTATTGGAGCACTTTGAAGATAGATATTT TCAGAAAAGATGTAGGATTTAAAAGTTAAATTTTAAATTTTAGAAAAAGATATGATGGCAATTGGAAATA GTCACAATGAAGTTCTTCATCCAGTAGGTGTTTAACAGTGTTATTTTGCCACTGGTAATGTGTAAACTGT GAGTGATTTACAATAAATGATTATGAATTCAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL831891 unedited GTCAGAATTTGTATACGACTCATATAGGCGGCNCGCGATTCGGCACGAGGGCCACTCACT CTGCTCTGGGCCTACTTCTGCTTCCTCCTGGCCGCTCTGGGTGTGACAGCTGGTGCCCAT CGCTTGTGGAGCCACAGGTCCTACCGGGCCAAGCTGCCTCTGAGGATATTTCTGGCTGTC GCCAACTCCATGGCTTTCCAGAATGACATCTTCGAGTGGTCCAGGGACCACCGAGCCCAC CACAAGTACTCAGAGACGGATGCTGACCCCCACAATGCCCGCCGGGGCTTCTTCTTCTCC CATATTGGGTGGCTGTTTGTTCGCAAGCATCGAGATGTTATTGAGAAGGGGAGAAAGCTT GACGTCACTGACCTGCTTGCTGATCCTGTGGTCCGGATCCAGAGAAAGTACTATAAGATC TCCGTGGTGCTCATGTGCTTTGTGGTCCCCACGCTGGTGCCCTGGTACATCTGGGGAGAG AGTCTGTGGAATTCCTACTTCTTGGCCTCTATTCTCCGCTATACCATCTCACTCAACATC AGCTGGCTGGTCAACAGCGCCGCCCACATGTATGGAAACCGGCCCTATGACAAGCACATC AGCCCTCGGCAGAACCCACTCGTCGCTCTGGGTGCCATTGGTGAAGGCTTCCATAATTAC CATCACACCTTTCCCTTTGACTACTCTGCGAGTGAATTTGGCTTANATTTTAACCCAACC ACCTGGTTCATTGATTTCATGTGCTGGCTGGNGCTGGCCACTGACCGCAAACGGGCAACC AAGCCGATGATCGAGGCCCCGAANGCCAGGACTGGAGACAGCAGTGCTTGAACTTGAACA GCCATCCCACATGTCTGCCGTGAACCTCGGTCATGGGCTTGGTACATAGCTCTCTGACAT TGNATCGNTGGAGGGGCACAGGTGGGGAAGGACAGTCAATGTGNTTGGGAATGTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL831891 unedited TTCTGTGAACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGAATTCTA ATCATTTATTGTAAATCACTCACAGTTTACACATTACCAGTGGCAAAATAACACTGTTAA ACACCTACTGGATGAAGAACTTCATTGTGACTATTTCCAATTGCCATCATATCTTTTTCT AAAATTTAAAATTTAACTTTTAAATCCTACATCTTTTCTGAAAATATCTATCTTCAAAGT GCTCCAATACTAACACTATAAGCCCTTTCTTTTGCTCTAACATCTAACACAAAGGGCACA CTGTCCCATTAATTCCACATGCACTTTACAAAGCAACTTCACACACAAATTTTTTTAATC CAGAAACTACATAATGATCACTTTATATTTTGCTACAAAATTAATGAAGGCTGCATGCAG GTGAAACATTAGTAGACACGGAATTCTAGATAACTGTTGGTTCTCAAGATCTGATGGCCT GATGTGGAAAATTAATATGCTTATTTAGCAGAAGTAGCTAAAGTACATGCATCAGTGGCT GGGATCAATTAGAATATAAATTAGGTGTTTAAACATTTAAACGAGAGCTTAATTTGAGCT TAATTTGATGTCTGGCATGTCAGAGTTATACACCTTTAAGATAACAATAGCAAACAGAAC ANAATCAAACATCTAAACAGGCATGATTTATAAGGTGGGCTGGGCATANGGCTCANGACA CANAACGAGCTGGGCAGTGAACAAATCTCTCTTTGAACAAAGACAGTAGAGCATTAACAA GCTGTAAACCCANGCAAAAATTAACCAGACCCATTTCTGGAATCTCATTTTTTCAGTTTT TGATTCTCTTAGCAACAATAAACCCACACACCCATAATTACAAACAGAACCTTCTTAGTA CACATAGTCCCCCTCTGTCTCTACCTGCCTCCTGCTCCTCAAAGGGTGGTTAAAGCATGA TGGG
Restriction Sites	NotI-NotI
ACCN	AL831891
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL831891.1, CAD38567.1
RefSeq Size	2287 bp
RefSeq ORF	2287 bp
Locus ID	79966
Protein Families	Transmembrane
Protein Pathways	Biosynthesis of unsaturated fatty acids, PPAR signaling pathway
Gene Summary	Stearoyl-CoA desaturase (SCD; EC 1.14.99.5) is an integral membrane protein of the endoplasmic reticulum that catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. SCD may be a key regulator of energy metabolism with a role in obesity and dislipidemia. Four SCD isoforms, Scd1 through Scd4, have been identified in mouse. In contrast, only 2 SCD isoforms, SCD1 (MIM 604031) and SCD5, have been identified in human. SCD1 shares about 85% amino acid identity with all 4 mouse SCD isoforms, as well as with rat Scd1 and Scd2. In contrast, SCD5 shares limited homology with the rodent SCDs and appears to be unique to primates (Wang et al., 2005 [PubMed 15907797]).[supplied by OMIM, Mar 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.