MEG3 (AF052114) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FP504; GTL2; LINC00023; NCRNA00023; onco-lncRNA-83; prebp1; PRO0518; PRO2160
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AF052114, the custom clone sequence may differ by one or more nucleotides ACAAAGCAGCCACTCACTGACCCCCAGGACCAGGATGGCAAAGGATGAAGAGGACCGGAACTGACCAGCC AGCTGTCCCTCTTACCTAAAGACTTAAACCAATGCCCTAGTGAGGGGGCATTGGGCATTAAGCCCTGACC TTTGCTATGCTCATACTTTGACTCTATGAGTACTTTCCTATAAGTCTTTGCTTGTGTTCACCTGCTAGCA AACTGGAGTGTTTCCCTCCCCAAGGGGGTGTCAGTCTTTGTCGACTGACTCTGTCATCACCCTTATGATG TCCTGAATGGAAGGATCCCTTTGGGAAATTCTCAGGAGGGGGACCTGGGCCAAGGGCTTGGCCAGCATCC TGCTGGCAACTCCAAGGCCCTGGGTGGGCTTCTGGAATGAGCATGCTACTGAATCACCAAAGGCACGCCC GACCTCTCTGAAGATCTTCCTATCCTTTTCTGGGGGAATGGGGTCGATGAGAGCAACCTCCTAGGGTTGT TGTGAGAATTAAATGAGATAAAAGAGGCCTCAGGCAGGATCTGGCATAGAGGAGGTGATCAGCAAATGTT TGTTGAAAAGGTTTGACAGGTCAGTCCCTTCCCACCCCTCTTGCTTGTCTTACTTGTCTTATTTATTCTC CAACAGCACTCCAGGCAGCCCTTGTCCACGGGCTCTCCTTGCATCAGCCAAGCTTCTTGAAAGGCCTGTC TACACTTGCTGTCTTCCTTCCTCACCTCCAATTTCCTCTTCAACCCACTGCTTCCTGACTCGCTCTACTC CGTGGAAGCACGCTCACAAAGGCACGTGGGCCGTGGCCCGGCTGGGTCGGCTGAAGAACTGCGGATGGAA GCTGCGGAAGAGGCCCTGATGGGGCCCACCATCCCGGACCCAAGTCTTCTTCCTGGCGGGCCTCTCGTCT CCTTCCTGGTTTGGGCGGAAGCCATCACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCCCA GCCCCTCTCCAGCTCGAAATCGGCAGACTAGGATGGAAGTGCCCTGTGAGCTGGGGGGCCCTTCAAAGGG CCAAGGAGAAAACGCAGGCCGAGGGACCAGCCTTCCAAATGGGCTTCAAGCTCCAATGACCTCCGCTCGC CCCCTCGAAATGTCTGGAAAACATAATGGGCAGATTTTCTGTCTTCAAAGTTTCCGGCTAAACCTCTTCA AGTTCTTTATTGTTTGGGACTGAGACACTCAGCCATGTTAATGGGTAGTTTCTTTTGTATTTGCCTTGAA AGGCCAAAATATTTTTATATTGCCACAGACAAAGCCACCTATTTAAAAATGAACTCCATGTCCGTCGTTT CCCACCAGGAGACTATGTACCATGTGTGTGTCTCTATGTATTCTGGGGTCTTGAAACAGGTTTCTCATGG GGATGGTCATTCACCACGGTCCAGAGGGGCAGAACAGGCGGCGCTTGCCTTGCCCAGGGGGCCTGGGGAA CGTGGGCCCTCATCTCAGATCTGCCCCCAGTATGTTTAGGACGCGAGCCCCAGAAGGATCTGGGAGTAAA CTTAACATTCACTGTGTCTCTGCTCTGCATCCGCCATTTGTGTGTGTTTCTGGACTGTGGGCTGTGTGTA CCTTGGTTGGTGACTCAGTGAGAAGAAGCAGGAATGCCAAAGATACTGTGAATGTTCTGAGTTTTGTTGC TGTTGTTGTTGAGAGGTTGTTTCACTGGTATCTATTGCATTGTATAATAAATGACCAGATGAATGAATGA GTGAAGCAAGAGAGAATGAATAAACAAGTAAATAGGTAAAGAAGTAAGCAAGCCAGGATGAGAGTGTGTG TACACAAGACCATGGTTCATCCGCTTTGATGGCTAGGCAATCAATATATAAATAGAAAAAAACCAGTGAA TCACTAAGTAATAGGGCAACACACAAAGCGATATCAGGTGATTATGGACTAAGGGGTATGTGTAACTCAA ATATATGCCTCTGACATTTGACAATGAAAAAGAACCTAAATGAAAGAAAGAATGGATGTATGAGTAGTGA AGTGCAGAATGAGACATAGATTTTGAGGCCCGTCAAAATGAAAAGATGCAAGTTAGGGAACAAGTGATCA AAAGGGAGAAGGGAAAGGTTTTTTTTAAAAAACCAAAACAACAAAGAAAGGTTTAAAAAAAAAACAGACT AGAGGATGAGTAATGAGTAACTCTGTAAGGAGGACCATGTCAGACTATTGTAAGCTAAGCATTAGGACTG ATACAAATAATATATGCTCCTGGCATAGAAAAATAAACCACAGAGAACGAGTTCAAAGAATAGCAAAGAA AGAAAGAGGACCCAGTGGGCGAAAGATGAGAGTGTACTTTTACCAAAAGTTATCTAAGCCTGAGCACTTG AAGTCTGCACATAAATAAATAAATGACAAAAGAAAGAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF052114 unedited CAGGAATTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCCCGGCTG GGTCGGCTGAAGAACTGCGGATGGAAGCTGCGGAAGAGGCCCTGATGGGGCCCACCATCC CGGACCCAAGTCTTCTTCCTGGCGGGCCTCTCGTCTCCTTCCTGGTTTGGGCGGAAGCCA TCACCTGGATGCCTACGTGGGAAGGGACCTCGAATGTGGGACCCCAGCCCCTCTCCAGCT CGAAATCGGCAGACTAGGATGGAAGTGCCCTGTGAGCTGGGGGGCCCTTCAAAGGGCCAA GGAGAAAACGCAGGCCGAGGGACCAGCCTTCCAAATGGGCTTCAAGCTCCAATGACCTCC GCTCGCCCCCTCGAAATGTCTGGAAAACATAATGGGCAGATTTTCTGTCTTCAAAGTTTC CGGCTAAACCTCTTCAAGTTCTTTATTGTTTGGGACTGAGACACTCAGCCATGTTAATGG GTAGTTTCTTTTGTATTTGCCTTGAAAGGCCAAAATATTTTTATATTGCCACAGACAAAG CCACCTATTTAAAAATGAACTCCATGTCCGTCGTTTCCCACCAGGAGACTATGTACCATG TGTGTGTCTCTATGTATTCTGGGGTCTTGAAACAGGTTTCTCATGGGGATGGTCATTCAC CACGGTCCAGAGGGGCAGAACAGGCGGCGCTTGCCTTGCCCAGNGGGCCTGGGGAAACGT GGCCCTCATCTCAGATCTGCCCCCAGTATGTTTAGGACGCGAGCCCCAGAAGGATCTGNG AGTAAACTTACATTCACTGTGTCTCTGCTCTGCATCCGCCATTTNNGTGTGTGTTCTGGA CTGTGGGCTGTGTGTACCTTGGTGGTGACTCAGTGAGAAGAAGCAGATGCCAAGAACTGT GATGTTCTGAGTTTTGTGCTGTGTGTTGAAAGGTGNTCACTGGATCTATTGCATGNATAT AAGGACGATGAATGATGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF052114 unedited NGGGTACAGCTATGNACCGCGGCACGCATNCTAGAGATCGAGTTTTTTTTTTTTTTTTTT GTTGTTTTGGTTTTTTAAAAAAAACTTTCCCTTCCCCTTTTGAACACTTGTTCCCTAACT TGCATCTTTTCATTTTGACGGGCCTCAAAATCTATGTCTCATTCTGCACTTCACTACTCA TACATCCATTCTTTCTTTCATTTAAGTTCTTTTTCATTGTCAAATGTCAGAGGCATATAT TTGAGTTACACATACCCCTTAGTCCATAATCACCTGATATCGCTTTGTGTGTTGCCCTAT TACTTAGTGATTCACTGGTTTTTTTCTATTTATATATTGATTGCCTAGCCATCAAAGCGG ATGAACCATGGTCTTGTGTACACACACTCTCATCCTGGCTTGCTTACTTCTTTACCTATT TACTTGTTTATTCATTCTCTCTTGCTTCACTCATTCATTCATCTGGTCATTTATTATACA ATGCAATAGATACCAGTGAAACAACCTCTCAACAACAACAGCAACAAAACTCAGAACATT CACAGTATCTTTGGCATTCCTGCTTCTTCTCACTGAGTCACCAACCAAGGTACACACAGC CCACAGTCCAGAAACACACACAAATGGCGGATGCAGAGCAGAGACACAGTGAATGTTAAG TTTACTCCCAGATCCTTCTGGGGCTCGCGTCCTAAACATACTGGGGGCAGATCTGAGATG AGGGCCCACGTTCCCCCAGCCCCCTGGGCAAGGCAAGCGCCGCCTGTTCTGCCCCTCTGG ACCGTGGTGAATGACCATCCCCATGAAGAAACTGTTTTCAAGACCCCCGNATACATAGAG ACACACACTGGTACATAGTCTCCCGGTGGGGAACGACGGACATGNAGTCCAT
Restriction Sites	NotI-NotI
ACCN	AF052114
Insert Size	1400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AF052114.1
RefSeq Size	2433 bp
RefSeq ORF	2433 bp
Locus ID	55384
Gene Summary	This gene is a maternally expressed imprinted gene. Multiple alternatively spliced transcript variants have been transcribed from this gene and all of them are long non-coding RNAs (lncRNAs). This gene is expressed in many normal tissues, but its expression is lost in multiple cancer cell lines of various tissue origins. It inhibits tumor cell proliferation in vitro. It also interacts with the tumor suppressor p53, and regulates p53 target gene expression. Its deletion enhances angiogenesis in vivo. Many experimental evidences demonstrate that this gene is a lncRNA tumor suppressor. [provided by RefSeq, Mar 2012]

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