CDC23 (BC010944) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	anaphase-promoting complex subunit 8; APC8; cell division cycle 23 homolog (S. cerevisiae); cell division cycle protein 23
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC010944, the custom clone sequence may differ by one or more nucleotides ATGGTCCCGGTGGCTGTGACGGCGGCAGTGGCGCCTGTCCTGTCCATAAACAGCGATTTCTCAGATTTGC GGGAAATTAAAAAGCAACTGCTGCTTATTGCGGGCCTTACCCGGGAGCGGGGCCTACTACACAGTAGCAA ATGGTCGGCGGAGTTGGCTTTCTCTCTCCCTGCATTGCCTCTGGCCGAGCTGCAACCGCCTCCGCCTATT ACAGAGGAAGATGCCCAGGATATGGATGCCTATACCCTGGCCAAGGCCTACTTTGACGTTAAAGAGTATG ATCGGGCAGCACATTTCCTGCATGGCTGCAATAGCAAGAAAGCCTATTTTCTGTATATGTATTCCAGATA TCTGGTGAGGGCCATTTTAAAATGTCATTCTGCCTTTAGTGAAACATCCATATTTAGAACCAATGGAAAA GTTAAATCTTTTAAATAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC010944 unedited NGGGTCACATTGATACACTATTAGGGGCCGGATTCGCCGAGGGAAAATGGCTGCGAGTAC CTCCATGGTCCCGGTGGCTGTGACGGCGGCAGTGGCGCCTGTCCTGTCCATAAACAGCGA TTTCTCAGATTTGCGGGAAATTAAAAAGCAACTGCTGCTTATTGCGGGCCTTACCCGGGA GCGGGGCCTACTACACAGTAGCAAATGGTCGGCGGAGTTGGCTTTCTCTCTCCCTGCATT GCCTCTGGCCGAGCTGCAACCGCCTCCGCCTATTACAGAGGAAGATGCCCAGGATATGGA TGCCTATACCCTGGCCAAGGCCTACTTTGACGTTAAAGAGTATGATCGGGCAGCACATTT CCTGCATGGCTGCAATAGCAAGAAAGCCTATTTTCTGTATATGTATTCCAGATATCTGTC TGGAGAAAAAAAGAAGGACGATGAAACAGTTGATAGCTTAGGCCCCCTGGAAAAAGGACA AGTGAAAAATGAGGCGCTTAGAGAATTGAGAGTGGAGCTCAGCAAAAAACACCAAGCTCG AGAACTTGATGGATTTGGACTTTATCTGTATGGTGTGGTGCTTCGAAAACTGGACTTGGT TAAAGAGGCCATTGATGTGTTTGTGGAAGCTACTCATGTTTTGCCCTTGCATTGNGGAGC CTGGTTAGAACTCTGTAACCTGATCACAGACAAAGAGATGCTGAAGTTCCTGTCTTTGCC AGACACCTGGATGAAAGAAGTTTTTCTGGCTCATATATACACAGAGTTGCAGTTGATAGA GGAGGCCCTGCAAAGTATCAGAACTCATTGATGTGGGCTTTCTCTAGAGCTCGTATATTG TTTCCCAAATTGCAGTTGCCTATCACATATCAGAGATATTGACCAAGCCCTCTCCATTTT TATGAN
Restriction Sites	NotI-NotI
ACCN	BC010944
Insert Size	3500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC010944.1, AAH10944.1
RefSeq Size	1088 bp
RefSeq ORF	438 bp
Locus ID	8697
Domains	APC8
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
Gene Summary	The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008]

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