ch TOG (CKAP5) (BC017856) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ch-TOG; CHTOG; colonic and hepatic tumor over-expressed; colonic and hepatic tumor over-expressed protein; cytoskeleton associated protein 5; FLJ35359; KIAA0097; MSPS; TOG; TOGp
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC017856, the custom clone sequence may differ by one or more nucleotides GGCGGCCCAAGCGGTTTCAAACGGCTTAGAGCAGGCCGCTTGGTTCTGACCCAGCTGAGGAAATACTCTT AATTCTAAGGAAAACCTGGAAGCACAATGGGAGATGACAGTGAGTGGTTGAAACTGCCAGTTGATCAGAA ATGTGAACACAAGCTGTGGAAAGCAAGGTTAAGTGGGTATGAAGAGGCCCTGAAGATCTTCCAGAAAATA AAGGATGAAAAGAGCCCAGAGTGGTCCAAATTTTTAGGATTGATCAAAAAATTTGTCACTGATTCCAATG CAGTGGTTCAATTGAAAGGATTAGAAGCTGCACTTGTTTATGTTGAAAATGCCCATGTAGCAGGAAAAAC CACAGGAGAAGTTGTGTCAGGTGTTGTAAGTAAGGTGTTCAATCAACCTAAAGCTAAAGCCAAGGAGCTG GGCATAGAGATCTGTCTTATGTACATAGAGATTGAGAAAGGAGAGGCTGTTCAAGAAGAGCTCCTGAAAG GCTTGGACAATAAGAATCCCAAGATCATAGTGGCCTGTATAGAGACACTGAGGAAAGCCTTAAGTGAATT TGGTTCCAAAATCATCTTGCTTAAGCCAATTATCAAAGTGTTGCCAAAACTCTTTGAGTCTCGAGAGAAG GCTGTTCGAGATGAAGCCAAACTAATTGCTGTGGAGATTTACAGATGGATTCGGGATGCTCTGAGACCCC CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC017856 unedited NNGGGGTGCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGCGGG GTGTGCGGCGGCCCAAGCGGTTTCAAACGGCTTAGAGCAGGCCGCTTGGTTCTGACCCAG CTGAGGAAATACTCTTAATTCTAAGGAAAACCTGGAAGCACAATGGGAGATGACAGTGAG TGGTTGAAACTGCCAGTTGATCAGAAATGTGAACACAAGCTGTGGAAAGCAAGGTTAAGT GGGTATGAAGAGGCCCTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAGCCCAGAGTGG TCCAAATTTTTAGGATTGATCAAAAAATTTGTCACTGATTCCAATGCAGTGGTTCAATTG AAAGGATTAGAAGCTGCACTTGTTTATGTTGAAAATGCCCATGTAGCAGGAAAAACCACA GGAGAAGTTGTGTCAGGTGTTGTAAGTAAGGTGTTCAATCAACCTAAAGCTAAAGCCAAG GAGCTGGGCATAGAGATCTGTCTTATGTACATAGAGATTGAGAAAGGAGAGGCTGTTCAA GAAGAGCTCCTGANAGGCTTGGACAATAAGAATCCCAAGATCATAGTGGCCTGTATAGAG ACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTTGCTTAAGCCAATTATC AAAGTGTTGCCAAAACTCTTTGAGTCTCGAGAGAAGGCTGTTCGAGATGAAGCCAAACTA ATTGCTGTGGGAGATTACAGATGGATTCNGGATGCTCTGAGACCCCCATTACAAATATAA ACTCTGTTCAGTTGAAAAAAACTAGAAGAAGAATGGGTCANACTGCCAACAAGTGCTCCT AGACCTACTCCGATTCTTCCGTTCCACAAGAACTAGAAGCTAAATTGGACCACACAGTCT GCTGGTGGAGATGCTGN
Restriction Sites	NotI-NotI
ACCN	BC017856
Insert Size	7000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017856.1, AAH17856.1
RefSeq Size	731 bp
RefSeq ORF	731 bp
Locus ID	9793
Protein Families	Druggable Genome
Gene Summary	This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

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