COQ9 (BC001478) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C16orf49; coenzyme Q9 homolog; coenzyme Q9 homolog (S. cerevisiae); DKFZP434K046; DKFZp434K046
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC001478, the custom clone sequence may differ by one or more nucleotides GTGGACAGCAGAGGCGATTGCAGAAGGAGCCCAGTCTCTGGGTCTCTCCAGTGCAGCAGCCAGCATGTTC GGGAAGGATGGCAGTGAGCTAATACTGCATTTTGTGACCCAGTGCAATACCCGGCTCACACGTGTGCTAG AAGAGGAGCAGAAGCTGGTACAGTTGGGCCAGGCGGAGAAGAGGAAGACAGACCAGTTCCTGAGGGATGC AGTGGAAACCAGACTGAGAATGCTGATCCCATACATTGAGCACTGGCCCCGGGCCCTCAGCATCCTCATG CTCCCTCACAACATCCCGTCCAGCCTGAGCCTGCTCACCAGCATGGTGGATGACATGTGGCATTACGCTG GGGACCAGTCCACTGATTTTAACTGGTACACCCGCCGAGCCATGCTGGCTGCCATCTACAACACAACAGA GCTGGTGATGATGCAGGACTCCTCTCCAGACTTTGAGGACACTTGGCGCTTCCTGGAAAACCGGGTTAAT GATGCAATGAACATGGGCCACACTGCCAAGCAGGTAAAGTCCACAGGAGAGGCACTGGTGCAAGGACTCA TGGGTGCAGCAGTGACGCTCAAGAACTTGACAGGTCTAAACCAGCGTCGGTGAGAGGAAGGGGTATAAGC TACAATGCCTAGAAGAGAATGAGCGGACAGATTGAAAGAGCTTTGAAAAGTATAAGGTGCCATCCACATA ACCTGGTGTTCACGAGAACACACTAAAGGACTCCTGAGTCACTACCACAGCCACCTGGAAACCACAAGGC ATTTGATGCTACCGTTCTGGTCAGGGATTGGGCTGCTTCTTCAGTTCCTAATACCAGACCAAGCCTCCTG ATGCCTTTCTGCACTGCAACTGTGTGATTGAAAAATGAGATGTTCATCCAAGCAGTCAAGCCACAGAAAC CCAGCATGTCCCTGTCACAATCTCATGGGCACCTTGATCATGTCTTAACCTTCCCTTAACCTTGGGGCTC CCAAGCCAGAGTCAAGGTCTGACGCCACCTCAAGGTGACAGCTCATCTCCAGCACAGCACAGGCGTGTGC ACACAGAGGTGTTCCTTGCAGCCCCCTCCCTCTCAGGTGTCCTGAGATGCTGCTCCTGGGAGCCCCCTCA GAAAACTGCCTCACCTGAGACAAGTGCCTGCTGGACAGAGGTGTGATTCCAGGCCTGGTGTCACATGACA CCAGCATGCATTGCAGGATTATTAGTGTATTTTGAGTCTGTAAAAATAATAAATATGTTTGAAGTAAAAA AAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC001478 unedited TCAAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTTGGATA AGTGGTTCTACAGGAGTCTCTGGCCTGCTTTTCCTCCTCCTTATCTGTGTTACCACTCTG GGTCTGAAAGGGTCTTCTCTGTTGAATGTCCCTGACTGACCAGTTTCTGTTTCAGTGGCC CGTTGCCGACAAGCCCTGGTGCCGCGTGCCTTCCATGCTTCAGCTGTGGGGCTAAGGTCT TCAGATGAGCAGAAGCAGCAGCCTCCCAACTCATTTTCTCAGCAGCATTCTGAGACACAG GGGGCAGAAAAACCTGATCCAGAGTCTTCTCATTCACCCCCCAGGTATACAGACCAGGGC GGCGAGGAGGAGGAGGACTATGAAAGTGAGGAGCAGTTGCAGCACCGCATCCTGACGGCA GCCCTTGAGTTTGTGCCCGCCCACGGGTGGACAGCAGAGGCGATTGCAGAAGGAGCCCAG TCTCTGGGTCTCTCCAGTGCAGCAGCCAGCATGTTCGGGAAGGATGGCAGTGAGCTAATA CTGCATTTTGTGACCCAGTGCAATACCCGGCTCACACGTGTGCTAGAAGAGGAGCAGAAG CTGGTACAGTTGGGCCAGGCGGAGAAGAGGAAGACAGACCAGTTCCTGAGGGATGCAGTG GAAACCAGACTGAGAATGCTGATCCCATACATTGAGCACTGGCCCCGGGTACCAAGTCTA TATCCAGGCCCCAGAGCAACAATAATCCTAATATTTATCATTCTCAGCACCTTTCACTCA GATGACTNTGTACACTGTTCAGAACTTAGCTTCTCATCTCTATAAACCCCGACATACTTA GTACACTTNGGTAAAAGCTGGTTTCAAACTTGNACATACCAAAGAGAGTGACTGAGCCCA TCTGTGTCTCAAGTCTTGGAATGTGAGTGGGCCGGTTACTGGATCATCNCTGGGTGGGGT CTCCTNATGGCCTGATGTGCCACCAGTCTGCAGGG
Restriction Sites	NotI-NotI
ACCN	BC001478
Insert Size	4700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC001478.1, AAH01478.1
RefSeq Size	1282 bp
RefSeq ORF	336 bp
Locus ID	57017
Protein Families	Druggable Genome
Gene Summary	This locus represents a mitochondrial ubiquinone biosynthesis gene. The encoded protein is likely necessary for biosynthesis of coenzyme Q10, as mutations at this locus have been associated with autosomal-recessive neonatal-onset primary coenzyme Q10 deficiency.[provided by RefSeq, Sep 2010]

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