Lamin B2 (LMNB2) (BC006551) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LAMB2; lamin B2; LMN2; MGC2721
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC106163 sequence for BC006551 edited (data generated by NextGen Sequencing) ATGGCCACGCCGCTGCCCGGCCGCGCGGGCGGGCCCGCCACGCCGCTGTCGCCCACGCGC CTGTCGCGGCTGCAGGAGAAGGAGGAGCTGCGCGAGCTCAACGACCGCCTGGCGCACTAC ATCGACCGCGTCCGCGCGCTGGAGCTGGAGAACGACCGGCTCCTGCTCAAGATCTCAGAG AAGGAGGAGGTGACCACGCGCGAGGTGAGTGGCATCAAGGCGCTGTACGAGTCGGAGCTG GCCGATGCCCGGAGAGTCCTGGATGAGACGGCTCGAGAGCGTGCCCGGCTGCAGATAGAG ATTGGGAAGCTGAGGGCAGAGTTGGACGAGGTCAACAAGAGCGCCAAGAAGAGGGAGGGC GAGCTTACGGTGGCCCAGGGCCGTGTGAAGGACCTGGAGTCCCTGTTCCACCGGAGCGAG GTGGAGCTGGCAGCTGCCCTCAGCGACAAGCGCGGCCTGGAGAGTGACGTGGCTGAGCTG CGGGCCCAGCTGGCCAAGGCCGAGGACGGTCATGCAGTGGCCAAAAAGCAGCTGGAGAAG GAGACGCTGATGCGTGTGGACCTGGAGAACCGCTGCCAGAGCCTGCAGGAGGAGCTGGAC TTCCGGAAGAGTGTGTTCGAGGAGGAGGTGCGGGAGACGCGGCGGCGGCACGAGCGGCGC CTGGTGGAGGTGGACAGCAGCCGGCAGCAGGAGTACGACTTCAAGATGGCACAGGCGCTG GAGGAGCTGCGGAGCCAGCACGACGAGCAAGTGCGGCTCTACAAGCTGGAGCTGGAGCAG ACCTACCAGGCCAAGCTGGACAGCGCCAAGCTGAGCTCTGACCAGAACGACAAGGCGGCC AGTGCGGCTCGCGAGGAGCTGAAGGAGGCCCGCATGCGCCTGGAGTCCCTCAGCTACCAG CTCTCCGGCCTCCAGAAGCAGGCCAGTGCCGCTGAAGATCGCATTCGGGAGCTGGAGGAG GCCATGGCCGGGGAGCGGGACAAGTTCCGGAAGATGCTGGACGCCAAGGAGCAGGAGATG ACGGAGATGCGGGACGTGATGCAGCAGCAGCTGGCCGAGTACCAGGAGCTGCTGGACGTG AAGCTGGCCCTGGACATGGAGATCAACGCCTACCGGAAGCTCCTGGAGGGCGAGGAGGAG AGGCTGAAGCTGTCCCCCAGCCCATCCTCGCGCGTCACCGTCTCACGAGCCACCTCGAGC AGCAGCGGCAGCTTGTCCGCCACCGGGCGCCTGGGCCGCAGTAAGCGGAAGCGGCTGGAG GTGGAGGAGCCCTTGGGCAGCGGCCCAAGCGTCCTGGGCACGGGCACGGGTGGCAGCGGT GGCTTCCACCTGGCCCAGCAGGCCTCGGCCTCGGGCAGCGTCAGCATCGAGGAGATCGAC CTGGAGGGCAAGTTTGTGCAGCTCAAGAACAACTCGGACAAGGATCAGTCTCTGGGGAAC TGGAGAATCAAGAGGCAGGTCTTGGAGGGGGAGGAGATCGCCTACAAGTTCACGCCCAAG TACATCCTGCGCGCCGGCCAGATGGTCACGGTGTGGGCAGCTGGTGCGGGGGTGGCCCAC AGCCCCCCCTCGACGCTGGTGTGGAAGGGCCAGAGCAGCTGGGGCACGGGCGAGAGCTTC CGCACCGTCCTGGTTAACGCGGATGGCGAGGAAGTGGCCATGAGGACTGTGAAGAAGTCC TCGGTGATGCGTGAGAATGAGAATGGGGAGGAAGAGGAGGAGGAAGCCGAGTTTGGCGAG GAGGATCTTTTCCACCAACAGGGGGACCCGAGGACCACCTCAAGAGGCTGCTACGTGATG TGA Clone variation with respect to BC006551.2 1356 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC006551 unedited ACGTCANCATTTGTATACGACNACTATAGGGCGGCCGCGATTCGGCACCAGGGATTGAAT GAGCCCGCCGAGCCCGGGCCGCCGTCGGGAGCAGCGCATGCCGCGAGCCGCCGCCACCAT GGCCACGCCGCTGCCCGGCCGCGCGGGCGGGCCCGCCACGCCGCTGTCGCCCACGCGCCT GTCGCGGCTGCAGGAGAAGGAGGAGCTGCGCGAGCTCAACGACCGCCTGGCGCACTACAT CGACCGCGTCCGCGCGCTGGAGCTGGAGAACGACCGGCTCCTGCTCAAGATCTCAGAGAA GGAGGAGGTGACCACGCGCGAGGTGAGTGGCATCAAGGCGCTGTACGAGTCGGAGCTGGC CGATGCCCGGAGAGTCCTGGATGATACGGCTCGAGAGCGTGCCCGGCTGCAGATAGAGAT TGGGAAGCTGAGGGCAGAGTTGGACGAGGTCAACAAGAGCGCCAAGAAGAGGGAGGGCGA GCTTACGGTGGCCCAGGGCCGTGTGAAGGACCTGGAGTCCCTGTTCCACCGGAGCGAGGT GGAGCTGGCAGCTGCCCTCATCGACAAGCGCGGCCTGGAGAGTGACGTGGCTGATCTGCG GGCCCAGCTGGCCAAGGCCGATGACGGTCATGCAGTGGCCAAAAAGCAGCTGGAGAATGA GACGCTGATGCGTGTGGACCTGGAGAACCGCTGCCAGAGCCTGCATGATGAGCTGGACTT CCGGAATATTGTGTTCNATGATGATGTGCGGGATACNCNGCGGCGGCACGATCGGCGCCT GGTGGATGTGGACAGCATCCGGCAGCATGAGTACGACTTCAAGATGCACATGCGCTGGAT GATCTGCNGAGCCAGCACGACNAGCCAGTGCGGCTCTACAGCTGGAGCTGGAGCAGACCT ACCAGCCCAGCTGGCAGCGCCAGCTGAGCTCTGACAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC006551 unedited CTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTGATATAAAAATAGTTTTCAGTGGCTC TGGGTAAAGAAAGTGTGTGGATGAAGAGTGTGGGTTCACATCACGTAGCAGCCTCTTGAG GTGGTCCTCGGGTCCCCCTGTTGGTGGAAAAGATCCTCCTCGCCAAACTCGGCTTCCTCC TCCTCTTCCTCCCCATTCTCATTCTCACGCATCACCGAGGACTTCTTCACAGTCCTCATG GCCACTTCCTCGCCATCCGCGTTAACCAGGACGGTGCGGAAGCTCTCGCCCGTGCCCCAG CTGCTCTGGCCCTTCCACACCAGCGTCGAGGGGGGGCTGTGGGCCACCCCCGCACCAGCT GCCCACACCGTGACCATCTGGCCGGCGCGCAAGATGTACTTGGGCGTGAACTTGTAGGCG ATCTCCTCCCCCTCCAAGACCTGCCTCTTGATTCTCCAGTTCCCCAGAGACTGATCCTTG TCCGAGTTGTTCTTGAGCTGCACAAACTTGCCCTCCAGGTCGATCTCCTCGATGCTGACG CTGCCCGAGGCCGAGGCCTGCTGGGCCAGGTGGAAGCCACCGCTGCCACCCGTGCCCGTG CCCAGGACGCTTGGGCCGCTGCCCAAGGGCTCCTCCACCTCCAGCCGCTTCCGCTTACTG CGGCCCAAGCGCCCGGTGGCGGACAAGCTGCCGCTGCTGCTCGAGGTGGCTCGTGAGACG GTGACGCGCCGAGATGGGCTGGGGGACAGCTTTAGCCTCTCCTTCTTGCCCTCCAGGAGC TTCCGGTAGCGTTGATCTCCATGTCCAAGG
Restriction Sites	NotI-NotI
ACCN	BC006551
Insert Size	2000 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006551.2, AAH06551.1
RefSeq Size	1943 bp
RefSeq ORF	1803 bp
Locus ID	84823
Domains	IF_tail, filament
Gene Summary	This gene encodes a B type nuclear lamin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Mutations in this gene are associated with acquired partial lipodystrophy. [provided by RefSeq, May 2012]

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