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NSUN2 (NM_017755) Human Untagged Clone
CAT＃: SC106395
NSUN2 (untagged)-Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1
CNY 5,624.00
CNY 7,220.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MISU; MRT5; SAKI; TRM4 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC106395 sequence for NM_017755 edited (data generated by NextGen Sequencing)
ATGGGGCGGCGGTCGCGGGGTCGGCGGCTCCAGCAACAGCAGCGGCCGGAGGACGCGGAG GATGGCGCCGAGGGTGGTGGAAAGCGCGGCGAGGCGGGCTGGGAAGGAGGCTACCCCGAG ATCGTCAAGGAGAACAAGCTGTTCGAGCACTACTACCAGGAGCTCAAGATCGTGCCCGAG GGCGAGTGGGGCCAGTTCATGGACGCTCTCAGGGAGCCGCTCCCGGCCACTTTAAGAATT ACTGGTTACAAAAGCCACGCAAAAGAGATTCTCCATTGCTTAAAGAACAAATATTTTAAG GAATTGGAGGACCTGGAGGTGGACGGTCAGAAAGTTGAAGTTCCACAGCCACTGAGTTGG TATCCTGAAGAACTTGCCTGGCACACAAATTTAAGTCGAAAAATCTTGAGAAAATCGCCA CACTTGGAAAAGTTTCATCAGTTTCTAGTTAGTGAAACAGAATCTGGAAATATTAGTCGT CAAGAAGCTGTTAGCATGATCCCACCACTGCTCCTCAACGTGCGGCCTCATCATAAGATC TTAGATATGTGTGCAGCACCTGGCTCAAAGACCACACAGTTAATTGAAATGCTACATGCC GACATGAATGTCCCCTTTCCAGAGGGATTTGTTATTGCGAATGATGTGGACAACAAGCGC TGCTACCTGCTCGTCCATCAAGCCAAGAGGCTGAGCAGCCCCTGCATCATGGTGGTCAAC CATGATGCCTCCAGCATACCCAGGCTCCAGATAGATGTGGACGGCAGGAAAGAGATCCTC TTCTATGATCGAATTTTATGTGATGTCCCTTGCAGTGGAGACGGCACTATGAGAAAAAAC ATTGATGTTTGGAAAAAGTGGACCACCTTAAATAGCTTGCAGCTACATGGCTTACAGCTG CGGATTGCAACACGCGGGGCTGAACAGCTGGCTGAAGGTGGAAGGATGGTGTATTCCACG TGTTCACTAAACCCTATTGAGGATGAAGCAGTCATAGCATCTTTACTGGAAAAAAGTGAA GGTGCTTTGGAGCTTGCTGATGTGTCTAATGAACTGCCAGGGCTGAAGTGGATGCCTGGA ATCACACAGTGGAAGGTAATGACGAAAGATGGGCAGTGGTTTACAGACTGGGACGCTGTT CCTCACAGCAGACACACCCAGATCCGACCTACCATGTTCCCTCCGAAGGACCCAGAAAAG CTGCAGGCCATGCACCTGGAGCGATGCCTTAGGATATTACCCCATCATCAGAATACTGGA GGGTTTTTTGTGGCAGTATTGGTGAAAAAATCTTCAATGCCGTGGAATAAACGTCAGCCA AAGCTTCAGGGTAAATCTGCAGAGACCAGAGAAAGCACACAGCTGAGCCCTGCAGATCTC ACAGAAGGGAAACCCACAGATCCCTCTAAGCTGGAAAGTCCGTCATTCACAGGAACTGGT GACACAGAAATAGCTCATGCAACTGAGGATTTAGAGAATAATGGCAGTAAGAAAGATGGC GTGTGTGGTCCTCCTCCATCAAAGAAAATGAAGTTATTTGGATTTAAAGAAGATCCATTT GTATTTATTCCTGAAGATGACCCATTATTTCCACCTATTGAGAAATTTTATGCTTTGGAT CCTTCATTCCCAAGGATGAATTTGTTAACTCGGACTACAGAAGGGAAGAAAAGGCAGCTC TACATGGTTTCTAAGGAGTTGCGGAATGTGCTGCTGAATAACAGTGAGAAGATGAAGGTT ATTAACACAGGGATCAAAGTCTGGTGTAGAAATAACAGCGGTGAAGAGTTTGACTGTGCT TTCCGGCTGGCACAGGAGGGAATATATACATTGTATCCATTTATTAACTCAAGAATTATT ACTGTATCAATGGAAGATGTTAAGATACTGTTGACCCAGGAAAATCCCTTTTTTAGAAAA CTCAGCAGTGAGACCTACAGTCAAGCAAAGGACCTGGCAAAGGGAAGCATCGTGCTGAAG TATGAACCAGATTCTGCGAATCCAGACGCTCTGCAGTGTCCCATCGTCTTATGCGGATGG CGGGGAAAGGCCTCCATTCGAACTTTTGTGCCCAAGAATGAACGGCTTCATTATCTCAGG ATGATGGGGCTGGAGGTATTGGGAGAAAAGAAGAAGGAAGGGGTTATCCTCACAAATGAG AGTGCAGCCAGCACCGGACAGCCAGACAATGACGTGACTGAGGGACAGAGAGCAGGAGAG CCCAACAGCCCAGATGCAGAAGAGGCCAACAGTCCAGACGTGACAGCAGGCTGTGACCCG GCGGGGGTCCATCCACCCCGGTGA Clone variation with respect to NM_017755.5 1749 g=>a >OriGene 5' read for NM_017755 unedited
GGTCACCATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGCCGGAAGTC CCGGGAGGTGTAGGGCTAGAGTTCTGGCCGTGGCGGGCCGGTTTCTGCGTGCTGCGTGCG CGGCCGCGTGGGCTATGGGGCGGCGGTCGCGGGGTCGGCGGCTCCAGCAACAGCAGCGGC CGGAGGACGCGGAGGATGGCGCCGAGGGTGGTGGAAAGCGCGGCGAGGCGGGCTGGGAAG GAGGCTACCCCGAGATCGTCAAGGAGAACAAGCTGTTCGAGCACTACTACCAGGAGCTCA AGATCGTGCCCGAGGGCGAGTGGGGCCAGTTCATGGACGCTCTCAGGGAGCCGCTCCCGG CCACTTTAAGAATTACTGGTTACAAAAGCCACGCAAAAGAGATTCTCCATTGCTTAAAGA ACAAATATTTTAAGGAATTGGAGGACCTGGAGGTGGACGGTCAGAAAGTTGAAGTTCCAC AGCCACTGAGTTGGTATCCTGAAGAACTTGCCTGGCACACAAATTTAAGTCGAAAAATCT TGAGAAAATCGCCACACTTGGAAAAGTTTCATCAGTTTCTAGTTAGTGAAACAGAATCTG GAAATATTAGTCGTCAAGAAGCTGTTAGCATGATCCCACCACTGCTCCTCAACGTGCGGC CTCATCATAAGATCTTAGATATGTGTGCAGCACCTGGCTCAAGACCACACAGTTAATTGA AATGCTACATGCCGACATGAATGTCCCCTTTCCAGAGGGATTTGNTATTGCGAATGATGT GGACAACAGCGCTGCTACCTGCTCGTCCATCAAGCCAAGAGGCTGAGCAGCCCCTGCATC ATGGTGGTCAACCATGATGCCTCCAGCATACCCCAGCTTCAGATAGATGTGGACGGGCAG AAAGAGATCCTCTNCTATGATCGAATTTATGTGATGTCCTTGCAGTGAGACGCACTATGA AAAAN >OriGene 3' read for NM_017755 unedited
NGGTTCGCCCATCGGNNGATGGCACTTTCCAGGNCCAGNAAAGCACTGGGGNAGGGTCAC AGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTC GAGTTTTTTTTTTTTTTTTTTATATTTTATCAATTTTATTGAAATATTCCAAGGATCCCA ACCCCATTTAAAAATAAAAATTGTAAAGCACTCCATTCAATAAAAGCACATAAGTCCCCC TCAATAATTAGTATGACAATTCACGATACAGCTCTTACTCTGGGAGAGTTTATTTTACCC TTTATTCCAAAAGGCACAAAGTCATCTGAGGCCTCAGATATTAACCCCACTGCATGTTAA TGACACACCACTGAGGTGCAGCTCAATGTAATTATTAAAGCTTATAACACACTTCCCCAA GAATTTATAGATTCTTTCTATAAATAATAATTTAAAAAATACTGCACCTTAAGACCAATA CAGGCTTAACAAAAGACCTGAAATTTCTGCAAGGGCAGTTTTGTTTCTTGATAGAAGTAC AACTTTTGAAAGTCTATTCCCAGCAAAAGAAACACTAGACCCAGCTTGGCCAAAGAAACA AAATAAAACAAGTGATTTCTAACACGCTAAAAGAGTACATTTTCATCAGCTCCAAAGAAA GCAGTCCTGGTCATTCAGAAGGCTCCTATGATCCCACCAGTCTGCAGTCATTAGAAATAT ATGCTTTACAGGCCACAGGCTGCTCTGGATTTGGTTTCAGACACCAGTGACCAGAAGAAG CCAGTTTTGCGTGTGAGGGGGTGTGGGCCCCCGCTGCCTTGGGCCTGCTCACCGNGGTGG ATGGACCCCCGCCNGGTCACAGCCTGCTGTCACGTCTGGACTGTTGGCCTCTTCTGCATC TGGN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_017755 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017755.4, NP_060225.4 |
| RefSeq Size | 3089 bp |
| RefSeq ORF | 2304 bp |
| Locus ID | 54888 |
| UniProt ID | Q08J23 |
| Domains | Nol1_Nop2_Sun |
| Gene Summary | This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011] Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (1). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability
,Shobbir Hussain, Sandra Blanco Benavente, Elisabete Nascimento, Ilaria Dragoni, Agata Kurowski, Astrid Gillich, Peter Humphreys, and Michaela Frye,
J. Cell Biol., Jul 2009; 186: 27 - 40.
[NSUN2]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214459 | NSUN2 (Myc-DDK-tagged)-Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1 |
CNY 5,616.00 |
|
| RC214459L1 | Lenti ORF clone of Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, Myc-DDK-tagged |
CNY 8,016.00 |
|
| RC214459L2 | Lenti ORF clone of Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, mGFP tagged |
CNY 7,700.00 |
|
| RC214459L3 | Lenti ORF clone of Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, Myc-DDK-tagged |
CNY 8,016.00 |
|
| RC214459L4 | Lenti ORF clone of Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1, mGFP tagged |
CNY 8,016.00 |
|
| RG214459 | NSUN2 (tGFP-tagged) - Human NOP2/Sun domain family, member 2 (NSUN2), transcript variant 1 |
CNY 7,216.00 |
