TSFM (AF110399) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EFTS; EFTSMT
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC106436 sequence for AF110399 edited (data generated by NextGen Sequencing) ATGTCGCTGCTGCGGTCGCTGCGCGTGTTCCTGGTCGCGCGGACCGGGAGCTACCCGGCT GGGTCTCTTCTGCGTCAGTCGCCCCAGCCAAGGCACACATTTTATGCTGGGCCCCGTCTG TCTGCCTCGGCCTCCAGCAAGGAGCTCCTCATGAAGCTGCGGCGGAAAACAGGCTACTCC TTTGTAAATTGCAAGAAAGCTCTGGAGACTTGTGGCGGGGACCTCAAACAGGCAGAGATC TGGCTCCACAAGGAGGCCCAGAAGGAGGGCTGGAGCAAAGCTGCCAAGCTCCAAGGGAGG AAGACCAAAGAAGGCCTGATTGGGCTGTTGCAGGAAGGAAACACAACTGTATTAGTAGAG GTAAACTGTGAGACAGATTTTGTTTCTAGAAATTTAAAATTTCAACTGTTGGTCCAGCAA GTAGCCCTTGGAACCATGATGCATTGTCAGACCCTAAAGGATCAACCCTCTGCATACAGT AAAGGTTTCTTGAATTCCTCTGAGCTTTCTGGACTTCCAGCTGGGCCTGACAGAGAAGGC TCACTCAAGGATCAGTTGGCTTTAGCAATTGGAAAACTGGGAGAAAACATGATTCTTAAA CGAGCTGCATGGGTGAAGGTGCCATCTGGGTTCTACGTTGGCTCTTATGTCCACGGAGCA ATGCAGAGTCCCTCACTTCACAAGCTGGTGCTGGGGAAGTATGGGGCCCTGGTCATCTGT GAGACGTCTGAACAGAAAACAAACCTTGAAGACGTTGGCCGCCGCCTTGGGCAGCATGTG GTGGGCATGGCCCCCCTCTCTGTTGGCTCCCTGGACGATGAGCCTGGGGGAGAGGCAGAG ACTAAGATGCTGTCCCAGCCGTATTTGCTGGATCCCTCCATTACCTTGGGGCAGTATGTG CAGCCTCAGGGGGTGTCGGTAGTAGACTTTGTGCGGTTTGAATGTGGAGAAGGTGAAGAG GCAGCAGAAACTGAATAG Clone variation with respect to AF110399.1 30 t=>c 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AF110399 unedited NNTTGTCAAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGGCCCGCC GGNAGGTGTTTATCGCGGCTAGAGAGATGTCGCTGCTGCGGTCGCTGCGCGTGTTCCTGG TCGCGCGGACCGGGAGCTACCCGGCTGGGTCTCTTCTGCGTCAGTCGCCCCAGCCAAGGC ACACATTTTATGCTGGGCCCCGTCTGTCTGCCTCGGCCTCCAGCAAGGAGCTCCTCATGA AGCTGCGGCGGAAAACAGGCTACTCCTTTGTAAATTGCAAGAAAGCTCTGGAGACTTGTG GCGGGGACCTCAAACAGGCAGAGATCTGGCTCCACAAGGAGGCCCAGAAGGAGGGCTGGA GCAAAGCTGCCAAGCTCCAAGGGAGGAAGACCAAAGAAGGCCTGATTGGGCTGTTGCAGG AAGGAAACACAACTGTATTAGTAGAGGTAAACTGTGAGACAGATTTTGTTTCTAGAAATT TAAAATTTCAACTGTTGGTCCAGCAAGTAGCCCTTGGAACCATGATGCATTGTCAGACCC TAAAGGATCAACCCTCTGCATACAGTAAAGGTTTCTTGAATTCCTCTGAGCTTTCTGGAC TTCCAGCTGGGCCTGACAGAGAAGGCTCACTCAAGGATCAGTTGGCTTTAGCAATTGGAA AACTGGGAGAAAACATGATTCTTAAACGAGCTGCATGGGTGAAGGTGCCATCTGGGTTCT ACGTTGGCTCTTATGTCCACGGAGCAATGCAGAGTCCCTCACTTCACAAGCTGGTGCTGG GGAAGTATGGGGCCCTGGTCATCTGTGAGACGTCTGAACAGAANACAACCTTGAAGACGT GGCCGCCGCCTTGGCAGCATGTGTGGGCATGGCCCCCTCTCTGTGGCTCCTGACAAGAGC CCTGGGGGAGAGCANAACTANNAGCTGTCCAGCCTAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AF110399 unedited AGGTCCACTATGNACCGCGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTACGCAA ACAAGGAAAAAATATTTTTATTATACAACTATTTAATAATTTTACCCATGCATTCTCGGT CTGAAGAGGTTTGGGAAATATTCCTTTTTGTAATGATGTCCAGAGCTAAAAGTAAATATT CCTCCTGGGCCAAAAGTCTCTGGAACCTATTCAGTTTCTGCTGCCTCTTCACCTTCTCCA CATTCAAACCGCACAAAGTCTACTACCGACACCCCCTGAGGCTGCACATACTGCCCCAAG GTAATGGAGGGATCCAGCAAATACGGCTGGGACAGCATCTTAGTCTCTGCCTCTCCCCCA GGCTCATCGTCCAGGGAGCCAACAGAGAGGGGGGCCATGCCCACCACATGCTGCCCAAGG CGGCGGCCAACGTCTTCAAAGTTTGTTTTCTGTTCAGACGTCTCACAGATGACCAGGGCC CCATACTTCCCCAGCACCAGCTTGTGAAGTGAGGGACTCTGCATTGCTCCGTGGACATAA GAGCCAACGTAGAACCCAGATGGCACCTTCACCCATGCAGCTCGTTTAAGAATCATGTTT TCTCCCAGTTTTCCCATTGCTAAAGCCAACTGATCCTTGAGTGAGCCTTCTCTGTCAGGC CCAGCTGGGAGTCCAGAAAGCTCACAAGAATTTCAGGAAACTTTACTGTATGCAGAGGGG TGATCCCTTAGGGTCTGACAATGCATCATGGGTCCAAGGGCCTACTTGCTGGACCCAACA ATTGAAAATTTAAATTTNCAGAAACAAAATCTGGCCTACAGTTTACCCTTCTCTAACCAA TTGGGGTTTCCTTCCTGGAACAACCCCAAAAGGCCT
Restriction Sites	NotI-NotI
ACCN	AF110399
Insert Size	1250 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AF110399.1, AAD20224.1
RefSeq Size	982 bp
RefSeq ORF	978 bp
Locus ID	10102
Domains	UBA, EF_TS
Protein Families	Transcription Factors
Gene Summary	This gene encodes a mitochondrial translation elongation factor. The encoded protein is an enzyme that catalyzes the exchange of guanine nucleotides on the translation elongation factor Tu during the elongation step of mitchondrial protein translation. Mutations in this gene are associated with combined oxidative phosphorylation deficiency-3 syndrome. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2010]

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