SMCHD1 (BC006008) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC006008, the custom clone sequence may differ by one or more nucleotides GAATCGGTTCCCGGGTGATCCTCGCGCCTGCCGCTGCTCGGCCGCCGCCGCTGACGAGGAGCTGCAGCGC GCCGGGCCGAGGCCTCGAGCCGCCCCGGGAGCTGGAGCTGAAGGCGCCGCGCGGAGCGCGCACCTCAGCC CTGAGCCCGGCGGCGGCAGGCGTCGCTGTCTTTTCTCCTTTTCCCCAATATGGCAGCGGCGGACGGCGGC GGGCCTGGTGGGGCCTCTGTGGGGACTGAGGAGGATGGCGGAGGCGTCGGCCACAGGACGGTGTACTTGT TTGATCGGCGCGAAAAGGAGTCCGAGCTCGGGGACCGGCCTCTGCAGGTCGGGGAGCGCTCGGACTACGC GGGATTTCGCGCGTGTGTGTGTCAGACACTTGGCATTTCACCTGAAGAAAAATTTGTTATTACAACAACA AGTAGGAAAGAAATTACCTGTGATAATTTTGATGAAACTGTTAAAGATGGAGTCACCTTATACCTGCTAC AGTCGGTCAATCAGTTACTACTGACAGCTACGAAAGAACGAATTGACTTCTTACCTCACTATGACACACT GGTTAAAAGTGGCATGTATGAATATTATGCCAGTGAAGGACAAAATCCTTTGCCATTTGCTCTTGCGGAA TTAATTGACAATTCATTGTCTGCTACTTCTCGTAACATTGGGGTTAGAAGAATACAGATCAAATTGCTTT TTGATGAAACACAAGGAAAACCTGCTGTTGCAGTGATAGATAATGGAAGAGGAATGACCTCTAAACAGCT TAACAACTGGGCCGTGTATAGGTTGTCAAAATTCACAAGGCAAGGTGACTTTGAAAGTGATCATTCAGGA TATGTTCGTCCAGTACCAGTGCCACGCAGTTTAAATAGTGATATTTCCTATTTTGGTGTTGGGGGCAAGC AAGCTGTCTTCTTTGTTGGACAATCAGCCAGAATGATAAGCAAACCTGCAGATTCCCAAGATGTTCACGA GCTTGTGCTTTCTAAAGAAGATTTTGAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC006008 unedited ATTCGGCACGAGCGGCGCTGGTCGCGGGTCGCACCGTGAGGCTCGCGTGGGCGGCGATAG GCGCTGGGCCCGGGCCCGGTGAGGAGCGCGCCGCGCGTCCCCTTCTCCTCAGGAGTGGCG GGCCGCGGAAGTGACGTGGTGCACGGGCAGGAGCGCGTTTGAATCGGTTCCCGGGTGATC CTCCGCCTGCGCTGCTCGGCCGCCGCCGCTGACGAGGAGCTGCAGNGCGCCGGGCCGAGG CCTCGAGCCCCCCGGGAGCTGGAGCTGAAGGCGCCGCGCGGAGCGCGCACCTCAGCCCTG AGCCCGGCGGCGGCACGGTTCGCTGTCTTTTCTCCTTTTCCCCAATATGGCAGCGGCGGA CGGCGGCGGCCCTGGTGGGGCCTCTGTGGGGACTGAGGAGGATGGCGGAGGCGTCGGCCA CAGGACGGTGTACTTGTTTGATCGGCGCGAAAAGGAGTCCGAGCTCGGGGACCGGCCTCT GCANGTCGGGGAGCGCTCGGACTACGCGGGATTTCGCGCGTGTGTGTGTCAGACACTTGG CATTTCACCTGAAGAAAAATTTGTTATTACAACAACAAGTAGGAAAGAAATTACCTGTGA TAATTTTGATGAAACTGTTAAAGATGGAGTCACCTTATACCTGCTACAGTCGGTCAATCA GTTACTACTGACAGCTACAAAAGAACGAATTGACTTCTTAACTCACTATGAACCACTGGN TAAAAGTGGCATGTATGAATATTATGCCAGTGAAGGACAAAATCCTTTGCCATTTGCTCT A
Restriction Sites	NotI-NotI
ACCN	BC006008
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006008.1
RefSeq Size	1040 bp
RefSeq ORF	1040 bp
Locus ID	23347
Protein Families	Druggable Genome
Gene Summary	This gene encodes a protein which contains a hinge region domain found in members of the SMC (structural maintenance of chromosomes) protein family. [provided by RefSeq, Dec 2011]

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