Arylsulfatase J (ARSJ) (AL832949) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL832949, the custom clone sequence may differ by one or more nucleotides CGGACGCGTGGGCGGACGCGTGGGCAGCATTATGCCAACGATAATTTAACTAGAAAAAGTTCCTGAGAGA ATAACAAATCTGTGATTTTATTATGTCTTTTATCATGTTTTGCAGCTTTCAGATTCAGATGTCAATAATT TGTTTCTATGATCAGAGTATATAAATCCCATGCAAACATATCTCAGCATAACTGATTTTGTTTCTAAATA ATTAAACAGGATAAAATGTTATTTGTTGTAAGCTGTTGACATTTGTAAGGAAATTTTGTTTCACTGAATA TGTTTAGAGATGCCGCCAGAACATTAACCATTGTTTCATTTTTATGTAAGTTGAAGAAAAATGAAGCTAG ATAGCATGTTCTCCATTTGCAGTCTACAAAGGGGAATTTTATTGCTTAAATTAAAGTTTCATTTTCTGGC CTGTGCAAGAGACTTTTATATCTAAAATATGGATGTACGTTTTTCATATTTTAGAGTTCATTGTATGGAA AAATACCATCAAAGTTGACCAAAAGATTTTGAAAATCCTTACCAGTTGTTTGTCATATGTTAAAGTCTTA TGGTTAATTTTATTTATTTTATCTTGTTCTCTTGCTGGTTATTGGCAGACTCAGTCTTTCTGTTTTCACA AAGAACTCATGAAGAGGACGATAGGGAAACCCACGTATGCCTTTGAGGCTAGGGACTATGTTGTAAGTTC ACCTGTGATGGCCAGGTCATACAGTCATGGCACAGCCACTAACCCCATTCACAGCACCAAGACTGGGGAC CCAGAGGCACTTGTTATGCTTCCACACTACGAAATGAAATTCTTAATTGAGAGAACTGGACACCAGGAAA AAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL832949 unedited GGAACTGGTTCTTTTGATGATTTAAAGGAAAATCTCTGTTTTACCATAATCAGCAGTATG AATTTTATTTCTTACTCCTCAAACGTGTGCTCAGCAAATCTTTGTTAAAAAAAATTTCCC AAATGATGTGTTTATTTTCCTGAGGTTAAACCACATACATATACCCACTAGAAGCAATCG TAAAGAAATAGAGAAATAGTTTTGCTTCCCTCACTTTTGGTCATTCTGTTCTTAGGGTCA GAATTAATGTTCCTTCCTCTAGGACATAGCAGTGCAAAAAAAGAGGAAGCACAAGCTCAA AAAGCAAATCTAAAGTTTGGAGAAATAGATTTCATTTTTAAGCAGTCTCAACATGGATCT TGCATGGGACATCCATGGCATGGCTGACAGCTTCCGTGACCAACATGGTACAGCCCTTCC TACCTTCTCATTTATTCTACTTTGTAACTTAACAGCATTATGCCAACGATAATTTAACTA GAAAAAGTTCCTGAGAGAATAACAAATCTGTGATTTTATTATGTCTTTTATCATGTTTTG CAGCTTTCAGATTCAGAATGTCAATAATTTGTTTCTATGATCAGAGTATATAAATCCCAT GCAAACATATCTCAGCATAACTGATTTTGTTTCTAAATAATTAAACAGGATAAAATGTTA TTTGTTGTAAGCTGTTGACATTTGTAAGGAAATTTTGTTTACTGAATATGTTAGAGATGC CGCAGAACATAACCATTGTTTATTNNTATGTAAGTGAAGAAATGAAGCTGATAGCATGTT CTCATTTGCAGTCTCAAGGGGAATTTTATGGCTAAATTAAGTTCATTTNTGGGCTGTGCA AAACTTTATTCTAAAATGGG
Restriction Sites	NotI-NotI
ACCN	AL832949
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL832949.1
RefSeq Size	854 bp
RefSeq ORF	854 bp
Locus ID	79642
Protein Families	Druggable Genome, Secreted Protein
Gene Summary	Sulfatases (EC 3.1.5.6), such as ARSJ, hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules (Sardiello et al., 2005 [PubMed 16174644]).[supplied by OMIM, Mar 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.