United States
Germany
Japan
United Kingdom
China
PHF8 (NM_015107) Human Untagged Clone
CAT＃: SC107370
PHF8 (untagged)-Human PHD finger protein 8 (PHF8), transcript variant 2
CNY 7,136.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | JHDM1F; KDM7B; MRXSSD; ZNF422 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_015107, the custom clone sequence may differ by one or more nucleotides
ATGGCCTCGGTGCCGGTGTATTGCCTCTGCCGGCTGCCTTACGATGTGACCCGCTTCATGATCGAGTGTG ACATGTGCCAGGACTGGTTTCATGGCAGTTGTGTTGGTGTTGAAGAGGAGAAGGCTGCTGACATTGACCT CTACCACTGCCCCAACTGTGAAGTCTTGCATGGGCCCTCCATTATGAAAAAACGCCGTGGATCTTCAAAG GGGCATGATACACACAAGGGGAAACCAGTGAAGACCGGGAGCCCTACGTTCGTCAGAGAGCTCCGGAGTA GGACTTTTGACAGCTCAGATGAAGTGATTCTGAAGCCCACTGGAAATCAACTGACCGTGGAATTCCTGGA AGAAAATAGCTTCAGTGTGCCCATCCTGGTCCTGAAGAAGGATGGGTTGGGCATGACGCTGCCCTCGCCA TCATTCACTGTGAGGGATGTTGAACACTATGTTGGTTCTGACAAAGAGATTGATGTGATTGATGTGACCC GCCAGGCTGACTGCAAGATGAAGCTTGGTGATTTTGTGAAATACTATTACAGCGGGAAGAGGGAGAAAGT CCTCAATGTCATTAGTTTGGAATTCTCTGATACCAGACTTTCTAACCTTGTGGAGACACCGAAGATTGTT CGAAAGCTGTCATGGGTCGAAAACTTGTGGCCAGAGGAATGTGTCTTTGAGAGACCCAATGTACAGAAGT ACTGCCTCATGAGTGTGCGAGATAGCTATACAGACTTTCACATTGACTTTGGTGGCACCTCTGTCTGGTA CCATGTACTCAAGGGTGAAAAGATCTTCTACCTGATCCGCCCAACAAATGCCAATCTGACTCTCTTTGAG TGCTGGAGCAGTTCCTCTAATCAGAATGAGATGTTCTTTGGGGACCAGGTGGACAAGTGCTACAAGTGTT CCGTGAAGCAAGGACAGACACTTTTCATTCCCACAGGGTGGATCCATGCTGTGCTGACGCCTGTGGACTG CCTTGCCTTTGGAGGGAACTTCTTACACAGCCTTAACATCGAGATGCAGCTCAAAGCCTATGAGATTGAG AAGCGGCTGAGCACAGCAGACCTCTTCAGATTCCCCAACTTTGAGACCATCTGTTGGTATGTGGGAAAGC ACATCCTGGACATCTTTCGCGGTTTGCGAGAGAACAGGAGACACCCTGCCTCCTACCTGGTCCATGGTGG CAAAGCCTTGAACTTGGCCTTTAGAGCCTGGACAAGGAAAGAAGCTCTGCCAGACCATGAGGATGAGATC CCGGAGACAGTGCGAACCGTACAGCTCATTAAAGATCTGGCCAGGGAGATCCGCCTGGTGGAAGACATCT TCCAACAGAACGTTGGGAAGACGAGCAATATCTTTGGGCTGCAGAGGATCTTCCCAGCCGGCTCCATTCC CCTAACCAGGCCAGCCCATTCCACTTCAGTGTCCATGTCCAGGCTGTCACTGCCCTCCAAAAATGGTTCA AAGAAGAAAGGCCTGAAGCCCAAGGAACTCTTCAAGAAGGCAGAGCGAAAGGGCAAGGAGAGTTCAGCCT TGGGGCCTGCTGGCCAGTTGAGCTATAATCTCATGGACACATACAGTCATCAGGCACTGAAGACAGGCTC TTTCCAGAAAGCAAAGTTCAACATCACTGGTGCCTGCTTGAATGACTCAGATGACGACTCACCAGACTTG GACCTTGATGGAAATGAGAGCCCATTGGCCCTATTGATGTCTAACGGCAGTACGAAAAGGGTGAAGAGTT TATCCAAATCTCGGCGAACCAAGATAGCAAAGAAGGTAGACAAGGCTAGGCTGATGGCAGAACAGGTGAT GGAAGACGAATTTGACTTGGATTCAGATGATGAGCTGCAGATTGACGAGAGATTGGGAAAGGAGAAGGCG ACCCTGATAATAAGACCAAAATTTCCCCGGAAATTGCCCCGTGCGAAGCCTTGCTCTGACCCCAACCGAG TTCGTGAACCAGGAGAAGTTGAGTTTGACATTGAGGAGGACTATACAACAGATGAGGACATGGTGGAAGG GGTTGAAGGCAAGCTTGGGAATGGTAGTGGCGCTGGTGGCATTCTTGATCTGCTCAAGGCCAGCAGGCAG GTGGGGGGACCTGACTATGCTGCCCTCACCGAGGCCCCAGCTTCTCCCAGCACTCAGGAGGCCATCCAGG GCATGCTGTGCATGGCCAACCTGCAGTCCTCATCGTCCTCACCGGCTACCTCTAGCCTGCAGGCCTGGTG GACTGGGGGACAGGATCGAAGCAGTGGGAGCTCCAGCAGTGGGCTGGGCACAGTGTCTAACAGTCCTGCT TCCCAGCGCACCCCAGGGAAGCGGCCCATCAAGCGGCCAGCATACTGGAGAACCGAGAGCGAGGAGGAGG AGGAGAACGCCAGTCTGGATGAACAGGACAGCTTGGGAGCGTGCTTCAAGGATGCAGAGTATATCTATCC TTCACTGGAGTCTGATGATGATGACCCTGCTTTGAAATCTCGACCCAAGAAAAAGAAGAATTCAGATGAT GCTCCATGGAGTCCTAAAGCCCGCGTGACCCCAACTCTGCCGAAGCAGGACCGTCCTGTGCGTGAGGGGA CCCGGGTAGCCTCTATTGAGACAGGTTTGGCTGCAGCAGCTGCAAAGCTGGCCCAGCAGGAGCTACAGAA GGCCCAAAAGAAGAAATATATCAAGAAGAAGCCTTTGCTGAAGGAGGTAGAACAGCCTCGCCCTCAAGAC TCCAATCTCAGTCTGACAGTACCAGCCCCCACTGTGGCTGCCACACCACAACTTGTCACCTCCTCCTCAC CCCTGCCTCCTCCTGAGCCTAAACAAGAGGCCCTGTCAGGAAGTCTCGCTGACCATGAGTACACCGCTCG TCCCAATGCCTTTGGCATGGCCCAGGCAAACCGCAGCACCACACCTATGGCCCCCGGTGTCTTCTTGACC CAGCGGCGCCCTTCAGTTGGCTCCCAGAGCAATCAGGCAGGACAAGGAAAGCGTCCCAAAAAGGGCCTGG CCACAGCAAAGCAGAGACTCGGCCGTATCCTGAAAATCCACAGAAATGGCAAACTACTTCTGTGA >OriGene 5' read for NM_015107 unedited
ATTACCCCCGCCCGTTGACGCAAAGGGCGGTCGGCGTGTACGGTGGGAGGTCTATATAAG CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC GAATTCGGCACGAGGGGACGAGCCTCCGCTGAGGCGCTTCGCAGTCCCGGAGCTAGCCCG GCTGCCGGCGTGTCGCTGGGGCTGAGCTCCGCGGGCGTGGAGTCCTTGCAGCCCAAAGCA TGAGGAGGTCCCTGTAGGATTCTGGACTGAAGACGTTCTTGTCAGGTTTGGGGCGTGAGG AGGTTCCTGTCAGTTGGGGAAGCGTTAAGAGTCCTCTATCGTCCAGAGAGGACGCGTGCT GCCGCCTCCCGCCCCTCTTGACACGACGAACCTGGCCGGCCGCAGAACGCTCCAGGGCCG AGCGAAGATGGCCTCGGTGCCGGTGTATTGCCTCTGCCGGCTGCCTTACGATGTGACCCG CTTCATGATCGAGTGTGACATGTGCCAGGACTGGTTTCATGGCAGCTGTGTTGGTGTTGA AGAGGAGAAGGCTGCTGACATTGACCTCTACCACTGCCCCAACTGTGAAGTCTTGCATGG GCCCTCCATTATGAAAAAACGCCGTGGATCTTCACAGGGGCATGATACACACAAGGGGAA ACCAGTGAAGACCGGGAGCCCTACGTTCGTCAGAGAGCTCCGGAGTAGGACTTTTGACAG CTCAGATGAAGTGATTCTGAAGCCCACTGGCCATCAACTGACCGTGGCATTCCTGGCAGA CATAGCTTCAGTGTGCCCATCCTGGTCCTGAAGACGGANGGNTTGGGCATGACGCTGCCC TCGCCATCATTCACTGGNAGGGATGTTGAACCTCATGTTTGGTTCTGACAAGAGCATGAT GTGCACTGATGTGACCCGCCCAGCTGACTGC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_015107 |
| Insert Size | 6000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015107.1, NP_055922.1 |
| RefSeq Size | 5776 bp |
| RefSeq ORF | 3075 bp |
| Locus ID | 23133 |
| UniProt ID | Q9UPP1 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene is a histone lysine demethylase that preferentially acts on histones in the monomethyl or dimethyl states. The encoded protein requires Fe(2+) ion, 2-oxoglutarate, and oxygen for its catalytic activity. The protein has an N-terminal PHD finger and a central Jumonji C domain. This gene is thought to function as a transcription activator. Defects in this gene are a cause of syndromic X-linked Siderius type intellectual disability (MRXSSD) and over-expression of this gene is associated with several forms of cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214763 | PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
CNY 7,128.00 |
|
| RC214763L1 | Lenti-ORF clone of PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
CNY 9,528.00 |
|
| RC214763L2 | Lenti-ORF clone of PHF8 (mGFP-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
CNY 12,160.00 |
|
| RC214763L3 | Lenti-ORF clone of PHF8 (Myc-DDK-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
CNY 12,160.00 |
|
| RC214763L4 | Lenti-ORF clone of PHF8 (mGFP-tagged)-Human PHD finger protein 8 (PHF8), transcript variant 2 |
CNY 12,160.00 |
|
| RG214763 | PHF8 (tGFP-tagged) - Human PHD finger protein 8 (PHF8), transcript variant 2 |
CNY 11,310.00 |
