United States
Germany
Japan
United Kingdom
China
PINK1 (NM_032409) Human Untagged Clone
CAT＃: SC108012
PINK1 (untagged)-Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein
CNY 6,496.00
| Cited in 6 publications. |
Product images
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BRPK; PARK6 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_032409 edited
ATGGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCTGGGTCGAGCGCTGCTGCTGCGC TTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGCGGCCGGGCCCGGCGGCGGGCTGT GTCCGCGGGGAGCGTCCAGGCTGGGCCGCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGG CTCGGGCTCCCTAACCGTCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGCGG TTGCAGCGGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGCGGCCGGGCAGTC TTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGAAAAACAGGCGGAGAGCCGGCGG GCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGGGGCCT GACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAG TCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAG AACCTGGAGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCA CCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCAAGATG ATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCCAGGAG CTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCAGTCACTTACAGAAAA TCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCC TTCACCTCTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCC TCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAAC TATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCGCCGCC ATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAACAGGGCATCGCGCAC AGAGACCTGAAATCCGACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTG GTGATCGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTC AGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACG GCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAGTGGGA GCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCAC CTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCA GACGTGAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCC CGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAG AATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTTTGTTG GCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCT AACCTGGAGTGTGAAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCC CTGTGA >OriGene 5' read for NM_032409 unedited
NTTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGCCGGTGGTGGCGG CAGCGGCGGCTGCGGGGGCACCGGGCCGCGGCGCCACCATGGCGGTGCGACAGGCGCTGG GCCGCGGCCTGCAGCTGGGTCGAGCGCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGG CCTACGGCTTGGGGCGGCCGGGCCCGGCGGCGGGCTGTGTCCGCGGGGAGCGTCCAGGCT GGGCCGCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGGCTCGGGCTCCCTAACCGTCTCC GCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGCGGTTGCAGCGGCAGTTCGTGGTGC GGGCCTGGGGCTGCGCGGGCCCTTGCGGCCGGGCAGTCTTTCTGGCCTTCGGGCTAGGGC TGGGCCTCATCGAGGAAAAACAGGCGGAGAGCCGGCGGGCGGTCTCGGCCTGTCAGGAGA TCCAGGCAATTTTTACCCAGAAAAGCAAGCCGGGGCCTGACCCGTTGGACACGAGACGCT TGCAGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAGTCCATTGGTAAGGGCTGCAGTG CTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAGAACCTGGAGGTGACAAAGAGCA CCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCAGGAGCGAG CTNCGGGNGGCCCCTGCCTTCCCCTGGGCCTCAGGATGATGTGGAACATCTCGGCAGGNT CCTCCAGCGAAGCCATCTTGAAACACATGAGCCAGGAAGCTGGGTCCAGCGAGCCGAGTG CCCTTGCTTGGGGAGATTGGGAGCAGTCACTTACAGAAATCCCAGAGAGGTCCAAGCAAC TAGCCCCTCACCCACTAAATCCGGGTTCTCCGCGCCTTACCTCTTCGTTGCCGTGTTGCC AAGGGCCCTGGTCAT >OriGene 3' read for NM_032409 unedited
AGTCTTGNNACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTGCAGNNGGGAAGTGGGGTTTAATATTTCACAATTCCTGGAA AAAAGGCTAGTCAGGAGGGAAACCCTCAAGTTTAAATCCCCACACTTACTTACTTACTGG TCATCCGGCACTTTCGCTAAGGGAGAATAATAATTCAGTAGCTGGTCCAAAACCCACAGC TTGGGAACGCGGAGGCCCAGGCCTCATGTAATTTCCCACTCCCGTAACTGAACGTGCTGA CCCATGTTGATGTCTGCCTTCATCCAGGGGGGAAATCAAACATGCTACGCTGCCCCCTTT AAACGAGTTCCTCGTGTTAGGGGCAAAGGGCACAGCCAAACTCTGCCACTGACAGCCAGT GCCTTGGCCTGGATCCTCTATGGGGATGCAGGGCTACCCCTCCTTAGTCCAGCCTCATTT ACTAAACCAGCTTGGCCATTCACTTTTCAAATGTCTGGGAGCACAGGACTGCAGACTGAA CTCTCACTCAAGTTCTTTCATTTGCCAAGCCCGAAGGCTTTTCCGGGTAACCAGCCCTCT CTGCGCTGGGTTGGCTCCTGACTCCCCACCTCACCATTTACAGACCATTACGACACAGAG GATGCCAAGTTCTTTTAGTAATTCACCAGCTCCATGCCGGGACATCACAGGGGCTGCCCT CCATGAGCAAAGGGAGGAAGGGCTGCCCTGCAAAANCGTTTCACACTCCAGGGTAACCCG AAAGAGCATCTTCATTTTTTGTTTCCACACACACTTTTCTGGGAGGCCTGTTGCCAACAA AGTGGCGGGCCATTGTTGGGAGGAGCCACCACCCTCTTGGCTAACTCAGATNCTTCCAGG CTGAAAAATGTTCACCCAAAGCTTTAAAGAAGCCCATTGCGGT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032409 |
| Insert Size | 2710 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032409.1, NP_115785.1 |
| RefSeq Size | 2700 bp |
| RefSeq ORF | 1746 bp |
| Locus ID | 65018 |
| UniProt ID | Q9BXM7 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Parkinson's disease |
| Gene Summary | This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008] |
Citations (6)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
In Vitro Comparison of the Activity Requirements and Substrate Specificity of Human and Triboleum castaneum PINK1 Orthologues
,Aerts, L;Craessaerts, K;De Strooper, B;Morais, VA;,
PLoS ONE
,PubMed ID 26784449
[PINK1]
|
|
PINK1 Catalytic Activity is Regulated by Phosphorylation on Serines 228 and 402
,Aerts, L;Craessaerts, K;De Strooper, B;Morais, VA;,
J. Biol. Chem.
,PubMed ID 25527497
[PINK1]
|
|
Mitochondrial impairment increases FL-PINK1 levels by calcium-dependent gene expression
,Gomez-Sanchez, R;Gegg, ME;Bravo-San Pedro, JM;Niso-Santano, M;Alvarez-Erviti, L;Pizarro-Estrella, E;Guti?rrez-Mart?n, Y;Alvarez-Barrientos, A;Fuentes, JM;Gonzalez-Polo, RA;Schapira, AH;,
,PubMed ID 24184327
[PINK1]
|
|
The kinase domain of mitochondrial PINK1 faces the cytoplasm
,Chun Zhou, Yong Huang, Yufang Shao, Jessica May, Delphine Prou, Celine Perier, William Dauer, Eric A. Schon, and Serge Przedborski,
PNAS, Aug 2008; 105: 12022 - 12027.
[PINK1]
|
|
C-terminal truncation and Parkinson's disease-associated mutations down-regulate the protein serine/threonine kinase activity of PTEN-induced kinase-1
,Chou Hung Sim, Daisy Sio Seng Lio, Su San Mok, Colin L. Masters, Andrew F. Hill, Janetta G. Culvenor and Heung-Chin Cheng,
Hum Mol Genet. 2006 Nov 1;15(21):3251-62.
[PINK1]
|
|
Wild-type PINK1 Prevents Basal and Induced Neuronal Apoptosis, a Protective Effect Abrogated by Parkinson Disease-related Mutations
,Agnes Petit, Toshitaka Kawarai, Erwan Paitel, Nobuo Sanjo, Mary Maj, Michael Scheid, Fusheng Chen, Yongjun Gu, Hiroshi Hasegawa, Shabnam Salehi-Rad, Linda Wang, Ekaterina Rogaeva, Paul Fraser, Brian Robinson, Peter St George-Hyslop, and Anurag Tandon,
J. Biol. Chem. 2005 Oct 7;280(40):34025-32.
[PINK1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206970 | PINK1 (Myc-DDK-tagged)-Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein |
CNY 6,488.00 |
|
| RC206970L1 | Lenti ORF clone of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 8,888.00 |
|
| RC206970L2 | Lenti ORF clone of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 8,888.00 |
|
| RC206970L3 | Lenti ORF clone of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC206970L4 | Lenti ORF clone of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 8,888.00 |
|
| RG206970 | PINK1 (tGFP-tagged) - Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein |
CNY 8,088.00 |
|
| SC323548 | PINK1 (untagged)-Kinase deficient mutant (K219M) of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein |
CNY 6,496.00 |
