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PINK1 (NM_032409) Human Untagged Clone
CAT＃: SC323548
PINK1 (untagged)-Kinase deficient mutant (K219M) of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein
CNY 6,496.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BRPK; PARK6 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323548 sequence for NM_032409 edited (data generated by NextGen Sequencing)
ATGGCGGTGCGACAGGCGCTGGGCCGCGGCCTGCAGCTGGGTCGAGCGCTGCTGCTGCGC TTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGCGGCCGGGCCCGGCGGCGGGCTGT GTCCGCGGGGAGCGTCCAGGCTGGGCCGCAGGACCGGGCGCGGAGCCTCGCAGGGTCGGG CTCGGGCTCCCTAACCGTCTCCGCTTCTTCCGCCAGTCGGTGGCCGGGCTGGCGGCGCGG TTGCAGCGGCAGTTCGTGGTGCGGGCCTGGGGCTGCGCGGGCCCTTGCGGCCGGGCAGTC TTTCTGGCCTTCGGGCTAGGGCTGGGCCTCATCGAGGAAAAACAGGCGGAGAGCCGGCGG GCGGTCTCGGCCTGTCAGGAGATCCAGGCAATTTTTACCCAGAAAAGCAAGCCGGGGCCT GACCCGTTGGACACGAGACGCTTGCAGGGCTTTCGGCTGGAGGAGTATCTGATAGGGCAG TCCATTGGTAAGGGCTGCAGTGCTGCTGTGTATGAAGCCACCATGCCTACATTGCCCCAG AACCTGGAGGTGACAAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCA CCAGGAGAAGGGCAGGAGCGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCATGATG ATGTGGAACATCTCGGCAGGTTCCTCCAGCGAAGCCATCTTGAACACAATGAGCCAGGAG CTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTATGGAGCAGTCACTTACAGAAAA TCCAAGAGAGGTCCCAAGCAACTAGCCCCTCACCCCAACATCATCCGGGTTCTCCGCGCC TTCACCTCTTCCGTGCCGCTGCTGCCAGGGGCCCTGGTCGACTACCCTGATGTGCTGCCC TCACGCCTCCACCCTGAAGGCCTGGGCCATGGCCGGACGCTGTTCCTCGTTATGAAGAAC TATCCCTGTACCCTGCGCCAGTACCTTTGTGTGAACACACCCAGCCCCCGCCTCGCCGCC ATGATGCTGCTGCAGCTGCTGGAAGGCGTGGACCATCTGGTTCAACAGGGCATCGCGCAC AGAGACCTGAAATCCGACAACATCCTTGTGGAGCTGGACCCAGACGGCTGCCCCTGGCTG GTGATCGCAGATTTTGGCTGCTGCCTGGCTGATGAGAGCATCGGCCTGCAGTTGCCCTTC AGCAGCTGGTACGTGGATCGGGGCGGAAACGGCTGTCTGATGGCCCCAGAGGTGTCCACG GCCCGTCCTGGCCCCAGGGCAGTGATTGACTACAGCAAGGCTGATGCCTGGGCAGTGGGA GCCATCGCCTATGAAATCTTCGGGCTTGTCAATCCCTTCTACGGCCAGGGCAAGGCCCAC CTTGAAAGCCGCAGCTACCAAGAGGCTCAGCTACCTGCACTGCCCGAGTCAGTGCCTCCA GACGTGAGACAGTTGGTGAGGGCACTGCTCCAGCGAGAGGCCAGCAAGAGACCATCTGCC CGAGTAGCCGCAAATGTGCTTCATCTAAGCCTCTGGGGTGAACATATTCTAGCCCTGAAG AATCTGAAGTTAGACAAGATGGTTGGCTGGCTCCTCCAACAATCGGCCGCCACTTTGTTG GCCAACAGGCTCACAGAGAAGTGTTGTGTGGAAACAAAAATGAAGATGCTCTTTCTGGCT AACCTGGAGTGTGAAACGCTCTGCCAGGCAGCCCTCCTCCTCTGCTCATGGAGGGCAGCC CTGTGA Clone variation with respect to NM_032409.2 656 a=>t >OriGene 5' read for mutant NM_032409 unedited
CCGCCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCCGGTGGTGGCG GCAGCGGCGGCTGCGGGGGCACCGGGCCGCGGCGCCACCATGGCGGTGCGACAGGCGCTGGGCCGCGGCC TGCAGCTGGGTCGAGCGCTGCTGCTGCGCTTCACGGGCAAGCCCGGCCGGGCCTACGGCTTGGGGCGGCC GGGCCCGGCGGCGGGCTGTGTCCCGCGGGGGAGCGTCCAGGCTGGGCCGCAAGGAACCGGGCGGCGGGAG CCTTCCGCAAGGGTCCGGGCTCTCGGCTTTCCCTACCCTGCTCTCCGCTTTCTTTCCGCCAATCTCGTGT GGCCGGCCTGGGCGGGCGCCGGGTTGCAACCGCCCGTTTCTTGGTGGGCGGGCCTTGGGGGCTGGCGCGG GCCCCTGGGCGGCGGGGCATTTTTTTTGGGCCCTTCGGGCCTAGGGGTGGGGCCCCATTCGGGGAAAAAA CAGGGCGGAAAGCCCGGGGGGCGGGCCCGGGCCGTGGCAGGGAATTCCACCCATTTTTATACCGAAAAAA CCACCCGGGGCCAAACCCTTGAAACCAAAACCTTTTGGAGGGTTCTGGGGGGAAGGATTAGTTAGCGGGT CTCCAATGAAGGCGCCTCACATAACTAGGGAAACCACATCGCGAATC >SC323548 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
TGRGTGMAAGAGCACCGGGTTGCTTCCAGGGAGAGGCCCAGGTACCAGTGCACCAGGAGAAGGGCWGWAG CGAGCTCCGGGGGCCCCTGCCTTCCCCTTGGCCATCATGATGATGTGGAACATCTCGGCAGGTTCCTCCA GCGAAGCCATCTTGAACACAATGAGCCAGGAGCTGGTCCCAGCGAGCCGAGTGGCCTTGGCTGGGGAGTA TGGAGCAGTCACTTACAGAAAATCCAAGAGAGGTCCCAAGCAACT |
| Restriction Sites | Please inquire |
| ACCN | NM_032409 |
| Insert Size | 2710 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032409.1, NP_115785.1 |
| RefSeq Size | 2700 bp |
| RefSeq ORF | 1746 bp |
| Locus ID | 65018 |
| UniProt ID | Q9BXM7 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Parkinson's disease |
| Gene Summary | This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206970 | PINK1 (Myc-DDK-tagged)-Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein |
CNY 6,488.00 |
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| RC206970L1 | Lenti ORF clone of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
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| RC206970L2 | Lenti ORF clone of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, mGFP tagged |
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| RC206970L3 | Lenti ORF clone of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC206970L4 | Lenti ORF clone of Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 8,888.00 |
|
| RG206970 | PINK1 (tGFP-tagged) - Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein |
CNY 8,088.00 |
|
| SC108012 | PINK1 (untagged)-Human PTEN induced putative kinase 1 (PINK1), nuclear gene encoding mitochondrial protein |
CNY 6,496.00 |
