MGC4368 (NM_024510) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC108098 sequence for NM_024510 edited (data generated by NextGen Sequencing) ATGGGCCACCGCAGTGATGTGGAAGCCATCGCAAGCTCATCACCAGCTTCCTGGAGCTGC ACTGCCTTGAGAGCCCCACAGAGCTGTCTCAGAGCAGCGACAGTGAGGCCGGTGACCCTG CAAGCCAGAGCTGACAGCCCCACTGTGCCTGAGCCCGTGCACCGCCCACAGGACCCATGG CACATTCCCGGTGTGCCTGAGCCCGTGCACCGCCCACAGGACCCGTGGCACATTCCCGGT GTGCCTGAGCCCGTGCACCGCCCACAGGACCCGTGGCACATTCCCGGTGTGCCTGAGCCC GTGCACCRCCCACAGGACCCGTGGCACATTCCCGGTGTGCCTGAGCCCGTGCACCGCCCA CAGGACCCGTGGCCTTGGCTTCAGTTGGTGCCTCCAGCCGAGTTGGCCTATTGCCTGCTC ATGCTGCTGCTTGCACACTGCATGAAACAGCAGGCCAGACCAGGACATCCAGACTTTCTC CATCGTGAGGCCTGGGCCTGCCTTTCTGCAGCCGGAGTCTCGCCAGCCCTGGACTCCTGC TTTGGGCCACAGCAAGACCTCGGGCGAGTGGAGAGGCGGNNNNNNNNNNCCCTTGTGGGT GCTGATGCTGCATGTTGTCCCCGACACAGCGTCCTCTCCCTGGTGGACATCCCCAGCGGT CAGGGTGCTTCCCCAAGGCAGTGA Clone variation with respect to NM_024510.2 308 c=>r;580 c=>n;581 c=>n;582 c=>n;583 a=>n;584 c=>n;585 g=>n;586 c=>n;587 c=>n;588 g=>n;589 c=>n 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_024510 unedited ATACGACTCATTATAGGGCGGCCGCGAATTCGGCACGAGGGACCCCTCCTCCTGTAGAGA GTGGTGCTGCCCTCTCGGGATGTACCTGCAGGTGGAGACCCGCACCAGCTCCCGCCTCCA TCTGAAGAGGGCTCCAGGCATCCGGTCCTGGTCCCTGCTGGTTGATAACCTGGGCTGGAA GCTCTTCTACGTCACAGGCTGCCTGTTTGTGGCTGTGCAGAACTTGGAGGACTGGGAGGA AGCCATCTTCGACAAGAGCACAGGGAAGGTTGTTTTGAAGACGTTCAGCCTCTACAAGAA GCTGCTGACTCTTTTCAGAGCTGGCCACGACCAGGTGGTGGTCCTGCTCCATGATGTCCG TGATGTGAGCGTGGAGGAGGAGAAGGTCCGGTACTTCGGGAAAGGCTACATGGTGGTGCT CCGGCTTGCGACGGGCTTCTCCCACCCCCTCACGCAGAGTGCAGTCATGGGCCACCGCAG TGATGTGGAAGCCATCGCCAAGCTCATCACCAGCTTCCTGGAGCTGCACTGCCTTGAGAG CCCCACAGAGCTGTCTCAGAGCAGCGACAGTGAGGCCGGTGACCCTGCAAGCCAGAGCTG ACAGCCCCACTGTGCCTGAGCCCGTGCACCGCCCACAGGACCCATGGCACATTCCCGGTG TGCCTGAGCCCGTGCACCGCCCACAGGACCCGTGGCACATTCCCGGTGTGCCTGAGCCCG TGCACCGCCACAGGACCCGTGCACATTCCCGTGTGCCTGACCCGTGCCCGCCCACAGGAC CCGTGCACATTCCCGTGTGCCTGAGCCGTGCACCGCCACAGGACCCGTGCCTTGCTTCAG TTGGTGCCTCAGCCGAGATGCCTATGCCTGCCATGCTGCTGCTGCCACTGATGAACAGCA GNCAGACCAGACATCAGACTTCTCATGGAGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_024510 unedited GCGGCCGCTTTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTGACACGTGAAGGGTTATTT ATGGTTATGATGACCCTGTCCTGCAACGAGGGACTGGCAGCCACTACTGAGGAGGAGGGT CCCATCTCTCTCCTGTCGGCTTTCACCGAGGTCACAGCCAGACGTGGGGCAAAGGTGTTC CCTGTCCTACCCAGCCATTCCTGGGCCTGCCGCCTAGGGGCTCACAGGGCCCAGGAGTCC CCAGCTCACAGGCCAGGGCATCAGGCCAGGCGCGCTCGGTGCACACCGCACCTGGGAGGA CCTGGGTACACTCAGGAGACCAAGAGCACTGGCGGGTCAGGATGGTTGGCGTTCAGCTCC TACGGGGTGGGGAGAAGTCTGTACCCGAGAGCCCATCCCCCTCCTGCCAGGACTTCCCAG GTTCGAGAGAGGCTGGAACTCAATTTCTGCAGAAAATCTCCCAGTTTTTCCTGTTTGGTT AGTTTTTTTACAAAGACAGGATCTTGCTGAGCTGCCCAGGCTGGACTTGAACTCCTGGCC TCAAGCAATCCTCCCACCCTCGGACTCCGAAAGTGCTGGGATTACTGGCATGAACCACTG CGCCCGGCTGGAGCTCCCGGTTTTTAAGCCCTGCACGATACTATAAGAGCTGACCTTTCT TCTGGCCTCACAGCTTATGCCGAACCTGAGTGCGAAGAACATAGAGACCTTCCTGTGACG ACCGCTGTGGCAGAGTGGTCTATGCGCCGAGATCCTGCATTCAACAAGGATGCGGCTCCT CGGGGAAGGCAGCCTACCAAGTGTGGCTGCGACGTGCCAGACAGGTATGCCCGGGAGCCC ACCCCTACTCGCCTTGGGTATCACCTGACGCTCGGGAGTCCACCAGGAAATGACCTGTGT CTGGACCACTGCCCATCCACCCCCAAGGCACGGTCCGCTCACCTCTCTACCCCAGGACTG CCGCACCC
Restriction Sites	NotI-NotI
ACCN	NM_024510
Insert Size	1960 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_024510.2, NP_078786.2
RefSeq Size	2250 bp
RefSeq ORF	2250 bp
Locus ID	79415
Protein Families	Transmembrane
Gene Summary	Necessary for a stable expression of the CYBA and CYBB subunits of the cytochrome b-245 hetrodimer. Controls the phagocyte respiratory burst and is essential for innate immunity.[UniProtKB/Swiss-Prot Function]

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