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CYP8B1 (NM_004391) Human Untagged Clone
CAT＃: SC108802
CYP8B1 (untagged)-Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1)
CNY 5,608.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CP8B; CYP12 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_004391 edited
CGAGGCAAGGTCCAGGTGCTCAGACCTTTACATCCTGGACTTTCCAGGCCTCCCAAAGCT CTCTGGCACCCAGGGAACAGTGTGCGTGTCGAGAGCTTAATCCGCAGGAGCATAGCCATG GTTCTCTGGGGTCCAGTGCTGGGAGCTCTGCTGGTGGTCATTGCTGGATACCTGTGCCTG CCAGGGATGCTCCGACAACGCAGGCCATGGGAGCCCCCTCTGGACAAGGGTACCGTGCCC TGGCTTGGCCATGCCATGGCTTTCCGGAAGAATATGTTTGAATTTCTGAAGCGCATGAGG ACCAAGCATGGGGATGTGTTCACAGTGCAGCTAGGGGGCCAGTACTTCACCTTCGTCATG GACCCCCTCTCCTTTGGCCCCATCCTCAAGGACACACAGAGAAAACTAGACTTTGGGCAA TATGCAAAAAAACTGGTGCTGAAGGTATTTGGATACCGTTCAGTGCAAGGGGACCATGAG ATGATACACTCAGCCAGCACCAAGTATCTGAGGGGGGATGGCTTGAAGGATCTTAATGAG ACCATGCTGGACAGCCTGTCCTTTGTAATGCTGACGTCCAAAGGCTGGAGTCTGGATGCC AGTTGCTGGCATGAGGACAGCCTCTTTCGCTTCTGCTATTACATCTTGTTCACAGCTGGC TACCTGAGCTTGTTCGGCTACACGAAGGACAAGGAGCAGGACCTGCTACAGGCAGGAGAG TTATTCATGGAGTTCCGCAAGTTTGACCTTCTTTTCCCAAGGTTTGTCTACTCCCTGCTG TGGCCCCGGGAGTGGCTAGAAGTGGGCCGACTCCAGCGTCTCTTTCACAAGATGCTCTCC GTGAGCCACAGCCAGGAGAAGGAGGGCATCAGCAACTGGCTGGGCAACATGCTTCAGTTT CTGAGGGAGCAGGGGGTACCCTCAGCTATGCAGGACAAGTTCAACTTCATGATGCTCTGG GCCTCCCAGGGGAACACGGGGCCTACCTCTTTCTGGGCCCTCTTGTACCTCCTGAAGCAC CCAGAAGCTATTCGGGCTGTGAGGGAGGAAGCTACCCAGGTCCTGGGTGAGGCCAGGCTG GAGACCAAGCAGTCCTTTGCCTTCAAACTCGGTGCCCTGCAACACACCCCAGTTCTAGAC AGCGTGGTGGAGGAGACGCTGCGGCTGAGGGCTGCACCCACCCTCCTCAGGTTGGTTCAT GAAGACTATACCCTGAAGATGTCCAGTGGGCAGGAGTATCTGTTCCGCCATGGAGACATC CTGGCCCTCTTTCCCTACCTCTCAGTGCACATGGACCCTGACATCCACCCTGAGCCCACC GTCTTCAAGTACGATCGCTTCCTCAACCCTAATGGCAGCCGGAAAGTGGACTTCTTCAAG ACAGGCAAGAAGATCCACCACTACACCATGCCCTGGGGTTCGGGCGTTTCCATCTGCCCT GGGAGGTTCTTTGCACTCAGTGAGGTGAAGCTCTTTATCCTGCTTATGGTCACACACTTT GACTTAGAGTTGGTGGACCCTGACACACCACTACCCCATGTTGACCCGCAGCGCTGGGGT TTTGGCACCATGCAGCCCAGCCACGATGTGCGCTTCCGCTACCGCCTGCATCCTACAGAG TGAGCTTGGCCAAGCCAGCTGCAAACCTGGCCAGAGGAGTTCTATTGCATCTCTCACCTG TTCTCACCCCTCTGCAGCCCCAAGACCCCACTGGCCACCCCTCCCTCTGGTCCTGTGGCA CCCCCTACCTCTGTTCTGCCTGTCCTCGCTCTCTCCCCGCCTAGTCATCTGACAGGCTTA TCATTCTCTTTAAAATACCATCTCT >OriGene 5' read for NM_004391 unedited
GGTTCCCATTAGTATACGATTCCTATAGGGCGGCCGCGAATTCGCACGAGGCAAGGTCCA GGTGCTCAGACCTTTACATCCTGGACTTTCCAGGCCTCCCAAAGCTCTCTGGCACCCAGG GAACAGTGTGCGTGTCGAGAGCTTAATCCGCAGGAGCATAGCCATGGTTCTCTGGGGTCC AGTGCTGGGAGCTCTGCTGGTGGTCATTGCTGGATACCTGTGCCTGCCAGGGATGCTCCG ACAACGCAGGCCATGGGAGCCCCCTCTGGACAAGGGTACCGTGCCCTGGCTTGGCCATGC CATGGCTTTCCGGAAGAATATGTTTGAATTTCTGAAGCGCATGAGGACCAAGCATGGGGA TGTGTTCACAGTGCAGCTAGGGGGCCAGTACTTCACCTTCGTCATGGACCCCCTCTCCTT TGGCCCCATCCTCAAGGACACACAGAGAAAACTAGACTTTGGGCAATATGCAAAAAAACT GGTGCTGAAGGTATTTGGATACCGTTCAGTGCAAGGGGACCATGAGATGATACACTCAGC CAGCACCAAGTATCTGAGGGGGGATGGCTTGAAGGATCTTAATGAGACCATGCTGGACAG CCTGTCCTTTGTAATGCTGACGTCCAAAGGCTGGAGTCTGGATGCCAGTTGCTGGCATGA GGACAGCCTCTTTCGCTTCTGCTATTACATCTTGTTCACAGCTGGCTACCTGAGCTTGGT CGGCTACACCAAGGACAAGGAGCAGGACCTGCTTCCAGTCAGGATAGTTATTCCTTGGAG TTCCGCAAGTTTGACCTTCTTTTCCAAAGATTGGCTACCCCCTGCTGTGGCCCTCGGAGG GCTCTTAGTGGCCCGCCTCCCACGTCTTTTCACAGAAGCTCCTCCGGAGCCCCATCCCGA AAAGGGGCC >OriGene 3' read for NM_004391 unedited
GGCATTGGGTGACATTTATCCTCCTTTTAGGGAATGGNGGTCAGAATGAATAAAAACCAT GCCCCATTTGGCTCCAGTCTAGGGAAGTGGGCAGCAGGCAGAAACCAAGACACAGAAAGC AACGACACATAACAGATATGCTATGATACAAATGGTGCTGCAGGAGCTGCACAGTTCGGA GGCCATTTCTGGCCAGAGGATGGGATGCGTGAAATCCAGGAAGGCTTCCAGGAAGGAGTA GCTGGCACTGGGATGGGCAGGAACAGCAATGTCTCTACTGATGGTTTCATTGGAGAAAGC TGGCAAAGTTGAGAAGTTTGGAGACTAACCGTGACAAAATTTAAACATCAGGTATGTGAA GGCAAGGGGGACCAACTGTGACAAAATGTAAACGTCAAGTGTGTGAAGTCAAAGGGGAAC CAGGAAAGTAAAAGAGTGACAATCAGCAGTGCTTTTGGCAAAAAGGCCTCTGGCAGCAGT GTAGAAGAGAAACGCAGAGATCACAGGTATAAGAATGACAATAGGAGAGGGGAGGAGGGT ACATATTTCAGAGGCATCACAGGGGGGTGTACTGATAGGACGGGGGCCTGCTTGTATGAA GGTGAAAGAGGAGGAGGGAGAGTGTCTCTGGGAGATGGGGTGAATGGTAGTGTTCTGAGC AGAGAGAGCAGGGAAATCANGAGGAGGAGTGGTTCAGGGAAGAAGCAGGGGTTCAGATGC CAAGTTCTGCATTGGCCAAGCTGAGTCAGAAGTGCTGGGAGACTCCCATGTGGGTCTGGN GATAGAAAGAAGCTCTGAGTTCAGCAATGATCATGGAAACCCAAGGGNAGGTTTGACAGC ACCCTGAGGTGGGGGGAGACATGGCACTNGAGAGGNATCTTGNATGATNCCTACATTAAA GACATNCTGACTGGGCACAGTGGCTCATGCCTGTAATCCCAGAAACTTGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004391 |
| Insert Size | 4130 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004391.1, NP_004382.1 |
| RefSeq Size | 3951 bp |
| RefSeq ORF | 1506 bp |
| Locus ID | 1582 |
| UniProt ID | Q9UNU6 |
| Domains | p450 |
| Protein Families | Druggable Genome, P450, Transmembrane |
| Protein Pathways | Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis |
| Gene Summary | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220836 | CYP8B1 (Myc-DDK-tagged)-Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1) |
CNY 5,592.00 |
|
| RC220836L1 | Lenti-ORF clone of CYP8B1 (Myc-DDK-tagged)-Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1) |
CNY 7,992.00 |
|
| RC220836L2 | Lenti-ORF clone of CYP8B1 (mGFP-tagged)-Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1) |
CNY 6,270.00 |
|
| RC220836L3 | Lenti-ORF clone of CYP8B1 (Myc-DDK-tagged)-Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1) |
CNY 6,270.00 |
|
| RC220836L4 | Lenti-ORF clone of CYP8B1 (mGFP-tagged)-Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1) |
CNY 6,270.00 |
|
| RG220836 | CYP8B1 (tGFP-tagged) - Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1) |
CNY 4,850.00 |
