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Cathepsin B (CTSB) (NM_001908) Human Untagged Clone
CAT＃: SC109129
CTSB (untagged)-Human cathepsin B (CTSB), transcript variant 1
CNY 3,656.00
| Cited in 4 publications. |
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APPS; CPSB; RECEUP |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC109129 sequence for NM_001908 edited (data generated by NextGen Sequencing)
ATGTGGCAGCTCTGGGCCTCCCTCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGAGCAGG CCCTCTTTCCATCCCCTGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATACCACG TGGCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTTGAAGAGGCTATGTGGT ACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATGTTTACCGAGGACCTGAAGCTG CCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGAGATCAGA GACCAGGGCTCCTGTGGCTCCTGCTGGGCCTTCGGGGCTGTGGAAGCCATCTCTGACCGG ATCTGCATCCACACCAATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGCTCACA TGCTGTGGCAGCATGTGTGGGGACGGCTGTAATGGTGGCTATCCTGCTGAAGCTTGGAAC TTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCATGTAGGGTGCAGA CCGTACTCCATCCCTCCCTGTGAGCACCACGTCAACGGCTCCCGGCCCCCATGCACGGGG GAGGGAGATACCCCCAAGTGTAGCAAGATCTGTGAGCCTGGCTACAGCCCGACCTACAAA CAGGACAAGCACTACGGATACAATTCCTACAGCGTCTCCAATAGCGAGAAGGACATCATG GCCGAGATCTACAAAAACGGCCCCGTGGAGGGAGCTTTCTCTGTGTATTCGGACTTCCTG CTCTACAAGTCAGGAGTGTACCAACACGTCACCGGAGAGATGATGGGTGGCCATGCCATC CGCATCCTGGGCTGGGGAGTGGAGAATGGCACACCCTACTGGCTGGTTGCCAACTCCTGG AACACTGACTGGGGTGACAATGGCTTCTTTAAAATACTCAGAGGACAGGATCACTGTGGA ATCGAATCAGAAGTGGTGGCTGGAATTCCACGCACCGATCAGTACTGGGAAAAGATCTAA Clone variation with respect to NM_001908.3 >OriGene 5' read for NM_001908 unedited
GTCAAAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCTCCGCT GCGCGCAGGCTGGGCTGCAGGCTCTCGGCTGCAGCGCTGGGTGGATCTAGGATCCGGCTT CCAACATGTGGCAGCTCTGGGCCTCCCTCTGCTGCCTGCTGGTGTTGGCCAATGCCCGGA GCAGGCCCTCTTTCCATCCCCTGTCGGATGAGCTGGTCAACTATGTCAACAAACGGAATA CCACGTGGCAGGCCGGGCACAACTTCTACAACGTGGACATGAGCTACTTGAAGAGGCTAT GTGGTACCTTCCTGGGTGGGCCCAAGCCACCCCAGAGAGTTATGTTTACCGAGGACCTGA AGCTGCCTGCAAGCTTCGATGCACGGGAACAATGGCCACAGTGTCCCACCATCAAAGAGA TCAGAGACCAGGGCTCCTGTGGCTCCTGCTGGGCCTTCGGGGCTGTGGAAGCCATCTCTG ACCGGATCTGCATCCACACCAATGCGCACGTCAGCGTGGAGGTGTCGGCGGAGGACCTGC TCACATGCTGTGGCAGCATGTGTGGNGACGGCTGTAATGGTGGCTATCCTGCTGAAGCTT GGAACTTCTGGACAAGAAAAGGCCTGGTTTCTGGTGGCCTCTATGAATCCCATGTANGGT GCAGACCGTACTCCATCCCTCCCTGTGAGCACCACGTCAACGGCTCCCGGGCCCCATGCA CGGNGGNAGGNAGATACCNNCCAGTGTAGCAAGATCTGTGAGCCTGGCTACAGNCCGACC TACAACAGGACAAGCACTACNGATACAANTNCTACAGCGTCTCCATAGCGAGAAGACATC ATGGNCGAGACTACANAACGGNCCNGTGNAGGGAGCTTCTCTTGTATTCGGACTTCTGCT CTACAGTCAGATGTACCCACACGTACCGAGAATGAAGGGTGGNCATG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001908 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001908.3, NP_001899.1 |
| RefSeq Size | 3783 bp |
| RefSeq ORF | 1020 bp |
| Locus ID | 1508 |
| UniProt ID | P07858 |
| Domains | Pept_C1 |
| Protein Families | Druggable Genome, Protease |
| Protein Pathways | Antigen processing and presentation, Lysosome |
| Gene Summary | This gene encodes a member of the C1 family of peptidases. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin B light and heavy chains, which can dimerize to form the double chain form of the enzyme. This enzyme is a lysosomal cysteine protease with both endopeptidase and exopeptidase activity that may play a role in protein turnover. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer's disease, the most common cause of dementia. Overexpression of the encoded protein has been associated with esophageal adenocarcinoma and other tumors. Both Cathepsin B and Cathepsin L are involved in the cleavage of the spike protein from the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) upon its entry to the human host cell. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Sep 2020] Transcript Variant: This variant (1) represents the shortest transcript and encodes the longer isoform (1). Variants 1-5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
uPAR and cathepsin B-mediated compartmentalization of JNK regulates the migration of glioma-initiating cells
,null,
Stem cell research
,PubMed ID 24699410
[CTSB]
|
|
Cathepsin B and uPAR regulate self-renewal of glioma-initiating cells through GLI-regulated Sox2 and Bmi1 expression
,Sreelatha Gopinath, RamaRao Malla, Kiranmai Alapati, Bharathi Gorantla, Meena Gujrati, Dzung H. Dinh, and Jasti S. Rao,
Carcinogenesis, Mar 2013; 34: 550 - 559.
[CTSB]
|
|
Cathepsin B and uPAR knockdown inhibits tumor-induced angiogenesis by modulating VEGF expression in glioma
,R R Malla, S Gopinath, C S Gondi, K Alapati, D H Dinh, M Gujrati, J S Rao,
Cancer Gene Therapy (11 March 2011) doi:10.1038/cgt.2011.9 Original Article
[CTSB]
|
|
a5ß1-Integrin controls ebolavirus entry by regulating endosomal cathepsins
,Kathryn L. Schornberg, Charles J. Shoemaker, Derek Dube, Michelle Y. Abshire, Sue E. Delos, Amy H. Bouton, and Judith M. White,
PNAS, May 2009; 106: 8003 - 8008.
[CTSB]
|
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Other Versions
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|---|---|---|---|
| RC222289 | CTSB (Myc-DDK-tagged)-Human cathepsin B (CTSB), transcript variant 1 |
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| RC222289L1 | Lenti ORF clone of Human cathepsin B (CTSB), transcript variant 1, Myc-DDK-tagged |
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| RC222289L2 | Lenti ORF clone of Human cathepsin B (CTSB), transcript variant 1, mGFP tagged |
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| RC222289L3 | Lenti ORF clone of Human cathepsin B (CTSB), transcript variant 1, Myc-DDK-tagged |
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| RC222289L4 | Lenti ORF clone of Human cathepsin B (CTSB), transcript variant 1, mGFP tagged |
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| RG222289 | CTSB (tGFP-tagged) - Human cathepsin B (CTSB), transcript variant 1 |
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