Integrin beta 4 binding protein (EIF6) (NM_181469) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAB, EIF6, EIF3A, p27BBP, b(2)gcn
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_181469, the custom clone sequence may differ by one or more nucleotides ATGGCGGTCCGAGCTTCGTTCGAGAACAACTGTGAGATCGGCTGCTTTGCCAAGCTCACCAACACCTACT GTCTGGTAGCGATCGGAGGCTCAGAGAACTTCTACAGTGTGTTCGAGGGCGAGCTCTCCGATACCATCCC CGTGGTGCACGCGTCTATCGCCGGCTGCCGCATCATCGGGCGCATGTGTGTGGGAGACAGAAGAAATTCT GGCAGATGTGCTCAAGGTGGAAGTCTTCAGACAGACAGTGGCCGACCAGGTGCTAGTAGGAAGCTACTGT GTCTTCAGCAATCAGGGAGGGCTGGTGCATCCCAAGACTTCAATTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_181469 unedited TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGGCCTTGGTCC ACCCAGACTTGGACAGGGAGACAGAAGAAATTCTGGCAGATGTGCTCAAGGTGGAAGTCT TCAGACAGACAGTGGCCGACCAGGTGCTAGTAGGAAGCTACTGTGTCTTCAGCAATCAGG GAGGGCTGGTGCATCCCAAGACTTCAATTGAAGACCAGGATGAGCTGTCCTCTCTTCTTC AAGTCCCCCTTGTGGCGGGGACTGTGAACCGAGGCAGTGAGGTGATTGCTGCTGGGATGG TGGTGAATGACTGGTGTGCCTTCTGTGGCCTGGACACAACCAGCACAGAGCTGTCAGTGG TGGAGAGTGTCTTCAAGCTGAATGAAGCCCAGCCTAGCACCATTGCCACCAGCATGCGGG ATTCCCTCATTGACAGCCTCACCTGAGTCACCTTCCAAGTTGTTCCATGGGCTCCTGGCT CTGGACTGTGGCCAACCTTCTCCACATTCCGCCCAATCTGTACCGGATGCTGGCAGGGAG GTGGCAGAGAGCTCACTGGGACTGAGGGGCTGGGCACCCAACCCTTTTCCACCTGTGCTT ATCGCCTGGATCTATCATTACTGCAAAAACCTGCTCTGTTGTGCTGGCTGGCANGCCCTG TGGCTGCTGGCTGAGGGTTCTGCTGTCCTGTGCCACCCCATTAAAGTGCAGTTCCCTTCG GNAAAAAAAANNAAAAAANNAAANNNANNNNNNAAAAANAANNNNNAAAAAAAAAAAANA AAAAANNAAAAAAAAAAAAAAAANNANNAAAAAAAAAAAAAACTCCCCTTTTAATGGGGC GCGGGTTATAATGGTCCCAAAACAACCC
Restriction Sites	NotI-NotI
ACCN	NM_181469
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_181469.1, NP_852134.1
RefSeq Size	1083 bp
RefSeq ORF	1083 bp
Locus ID	3692
Protein Families	Druggable Genome
Gene Summary	Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate exon compared to variant 1, which causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Both variants 3 and 5 encode isoform b.

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