ROBO1 (NM_133631) Human Untagged Clone
CAT#: SC109740
ROBO1 (untagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2
CNY 16,552.00
Cited in 2 publications. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DUTT1; SAX3 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_133631 edited
GAATTCGGCACCAGGCAAGACCGTGGAGGCAGGAAACGGCACTACTGCGCTTCTGCCTCG GCTCTTTGTTGTTCGCTTTGGATGGTTCTTGAAAGTGTCTGAGCCTCCTCGGAAATCCTG GGGCCGGAGAAGACAAACCTTGGAATTCTTCCTCTGCAAAAGTCTCTGAGATACTGACAA GCGTCCGGAAAGGTCGACGAGTAATTGCCCTGAAAACTCTTGGCTAATTGACCCACGTTG CTTATATTAAGCCTTTGTGTGTGGTGTGTGGCTTCATACATTTGGGGACCCTATTTCCAC TCCCTCCTCTTGGCATGAGACTGTATACAGGATCCACCCGAGGACAATGATTGCGGAGCC CGCTCACTTTTACCTGTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCTTCGTCAGGA AGATTTTCCACCTCGCATTGTTGAACACCCTTCAGACCTGATTGTCTCAAAAGGAGAACC TGCAACTTTGAACTGCAAAGCTGAAGGCCGCCCCACACCCACTATTGAATGGTACAAAGG GGGAGAGAGAGTGGAGACAGACAAAGATGACCCTCGCTCACACCGAATGTTGCTGCCGAG TGGATCTTTATTTTTCTTACGTATAGTACATGGACGGAAAAGTAGACCTGATGAAGGAGT CTATGTCTGTGTAGCAAGGAATTACCTTGGAGAGGCTGTGAGCCACAATGCATCGCTGGA AGTAGCCATACTTCGGGATGACTTCAGACAAAACCCTTCGGATGTCATGGTTGCAGTAGG AGAGCCTGCAGTAATGGAATGCCAACCTCCACGAGGCCATCCTGAGCCCACCATTTCATG GAAGAAAGATGGCTCTCCACTGGATGATAAAGATGAAAGAATAACTATACGAGGAGGAAA GCTCATGATCACTTACACCCGTAAAAGTGACGCTGGCAAATATGTTTGTGTTGGTACCAA TATGGTTGGGGAACGTGAGAGTGAAGTAGCCGAGCTGACTGTCTTAGAGAGACCATCATT TGTGAAGAGACCCAGTAACTTGGCAGTAACTGTGGATGACAGTGCAGAATTTAAATGTGA GGCCCGAGGTGACCCTGTACCTACAGTACGATGGAGGAAAGATGATGGAGAGCTGCCCAA ATCCAGATATGAAATCCGAGATGATCATACCTTGAAAATTAGGAAGGTGACAGCTGGTGA CATGGGTTCATACACTTGTGTTGCAGAAAATATGGTGGGCAAAGCTGAAGCATCTGCTAC TCTGACTGTTCAAGTTGGGTCTGAACCTCCACATTTTGTTGTGAAACCCCGTGACCAGGT TGTTGCTTTGGGACGGACTGTAACTTTTCAGTGTGAAGCAACCGGAAATCCTCAACCAGC TATTTTCTGGAGGAGAGAAGGGAGTCAGAATCTACTTTTCTCATATCAACCACCACAGTC ATCCAGCCGATTTTCAGTCTCCCAGACTGGCGACCTCACAATTACTAATGTCCAGCGATC TGATGTTGGTTATTACATCTGCCAGACTTTAAATGTTGCTGGAAGCATCATCACAAAGGC ATATTTGGAAGTTACAGATGTGATTGCAGATCGGCCTCCCCCAGTTATTCGACAAGGTCC TGTGAATCAGACTGTAGCCGTGGATGGCACTTTCGTCCTCAGCTGTGTGGCCACAGGCAG TCCAGTGCCCACCATTCTGTGGAGAAAGGATGGAGTCCTCGTTTCAACCCAAGACTCTCG AATCAAACAGTTGGAGAATGGAGTACTGCAGATCCGATATGCTAAGCTGGGTGATACTGG TCGGTACACCTGCATTGCATCAACCCCCAGTGGTGAAGCAACATGGAGTGCTTACATTGA AGTTCAAGAATTTGGAGTTCCAGTTCAGCCTCCAAGACCTACTGACCCAAATTTAATCCC TAGTGCCCCATCAAAACCTGAAGTGACAGATGTCAGCAGAAATACAGTCACATTATCGTG GCAACCAAATTTGAATTCAGGAGCAACTCCAACATCTTATATTATAGAAGCCTTCAGCCA TGCATCTGGTAGCAGCTGGCAGACCGTAGCAGAGAATGTGAAAACAGAAACATCTGCCAT TAAAGGACTCAAACCTAATGCAATTTACCTTTTCCTTGTGAGGGCAGCTAATGCATATGG AATTAGTGATCCAAGCCAAATATCAGATCCAGTGAAAACACAAGATGTCCTACCAACAAG TCAGGGGGTGGACCACAAGCAGGTCCAGAGAGAGCTGGGAAATGCTGTTCTGCACCTCCA CAACCCCACCGTCCTTTCTTCCTCTTCCATCGAAGTGCACTGGACAGTAGATCAACAGTC TCAGTATATACAAGGATATAAAATTCTCTATCGGCCATCTGGAGCCAACCACGGAGAATC AGACTGGTTAGTTTTTGAAGTGAGGACGCCAGCCAAAAACAGTGTGGTAATCCCTGATCT CAGAAAGGGAGTCAACTATGAAATTAAGGCTCGCCCTTTTTTTAATGAATTTCAAGGAGC AGATAGTGAAATCAAGTTTGCCAAAACCCTGGAAGAAGCACCCAGTGCCCCACCCCAAGG TGTAACTGTATCCAAGAATGATGGAAACGGAACTGCAATTCTAGTTAGTTGGCAGCCACC TCCAGAAGACACTCAAAATGGAATGGTCCAAGAGTATAAGGTTTGGTGTCTGGGCAATGA AACTCGATACCACATCAACAAAACAGTGGATGGTTCCACCTTTTCCGTGGTCATTCCCTT TCTTGTTCCTGGAATCCGATACAGTGTGGAAGTGGCAGCCAGCACTGGGGCTGGGTCTGG GGTAAAGAGTGAGCCTCAGTTCATCCAGCTGGATGCCCATGGAAACCCTGTGTCACCTGA GGACCAAGTCAGCCTCGCTCAGCAGATTTCAGATGTGGTGAAGCAGCCGGCCTTCATAGC AGGTATTGGAGCAGCCTGTTGGATCATCCTCATGGTCTTCAGCATCTGGCTTTATCGACA CCGCAAGAAGAGAAACGGACTTACTAGTACCTACGCGGGTATCAGAAAAGTCCCGTCTTT TACCTTCACACCAACAGTAACTTACCAGAGAGGAGGCGAAGCTGTCAGCAGTGGAGGGAG GCCTGGACTTCTCAACATCAGTGAACCTGCCGCGCAGCCATGGCTGGCAGACACGTGGCC TAATACTGGCAACAACCACAATGACTGCTCCATCAGCTGCTGCACGGCAGGCAATGGAAA CAGCGACAGCAACCTCACTACCTACAGTCGCCCAGCTGATTGTATAGCAAATTATAACAA CCAACTGGATAACAAACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGT GGACCTTAGTAACAAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGATGG GCGTTTTGTCAATCCATCAGGGCAGCCTACTCCTTACGCCACCACTCAGCTCATCCAGTC AAACCTCAGCAACAACATGAACAATGGCAGCGGGGACTCTGGCGAGAAGCACTGGAAACC ACTGGGACAGCAGAAACAAGAAGTGGCACCAGTTCAGTACAACATCGTGGAGCAAAACAA GCTGAACAAAGATTATCGAGCAAATGACACAGTTCCTCCAACTATCCCATACAACCAATC ATACGACCAGAACACAGGAGGATCCTACAACAGCTCAGACCGGGGCAGTAGTACATCTGG GAGTCAGGGGCACAAGAAAGGGGCAAGAACACCCAAGGTACCAAAACAGGGTGGCATGAA CTGGGCAGACCTGCTTCCTCCTCCCCCAGCACATCCTCCTCCACACAGCAATAGCGAAGA GTACAACATTTCTGTAGATGAAAGCTATGACCAAGAAATGCCATGTCCCGTGCCACCAGC AAGGATGTATTTGCAACAAGATGAATTAGAAGAGGAGGAAGATGAACGAGGCCCCACTCC CCCTGTTCGGGGAGCAGCTTCTTCTCCAGCTGCCGTGTCCTATAGCCATCAGTCCACTGC CACTCTGACTCCCTCCCCACAGGAAGAACTCCAGCCCATGTTACAGGATTGTCCAGAGGA GACTGGCCACATGCAGCACCAGCCCGACAGGAGACGGCAGCCTGTGAGTCCTCCTCCACC ACCACGGCCGATCTCCCCTCCACATACCTATGGCTACATTTCAGGACCCCTGGTCTCAGA TATGGATACGGATGCGCCAGAAGAGGAAGAAGACGAAGCCGACATGGAGGTAGCCAAGAT GCAAACCAGAAGGCTTTTGTTACGTGGGCTTGAGCAGACACCTGCCTCCAGTGTTGGGGA CCTGGAGAGCTCTGTCACGGGGTCCATGATCAACGGCTGGGGCTCAGCCTCAGAGGAGGA CAACATTTCCAGCGGACGCTCCAGTGTTAGTTCTTCGGACGGCTCCTTTTTCACTGATGC TGACTTTGCCCAGGCAGTCGCAGCAGCGGCAGAGTATGCTGGTCTGAAAGTAGCACGACG GCAAATGCAGGATGCTGCTGGCCGTCGACATTTTCATGCGTCTCAGTGCCCTAGGCCCAC AAGTCCCGTGTCTACAGACAGCAACATGAGTGCCGCCGTAATGCAGAAAACCAGACCAGC CAAGAAACTGAAACACCAGCCAGGACATCTGCGCAGAGAAACCTACACAGATGATCTTCC ACCACCTCCTGTGCCGCCACCTGCTATAAAGTCACCTACTGCCCAATCCAAGACACAGCT GGAAGTACGACCTGTAGTGGTGCCAAAACTCCCTTCTATGGATGCAAGAACAGACAGATC ATCAGACAGAAAAGGAAGCAGTTACAAGGGGAGAGAAGTGTTGGATGGAAGACAGGTTGT TGACATGCGAACAAATCCAGGTGATCCCAGAGAAGCACAGGAACAGCAAAATGACGGGAA AGGACGTGGAAACAAGGCAGCAAAACGAGACCTTCCACCAGCAAAGACTCATCTCATCCA AGAGGATATTCTACCTTATTGTAGACCTACTTTTCCAACATCAAATAATCCCAGAGATCC CAGTTCCTCAAGCTCAATGTCATCAAGAGGATCAGGAAGCAGACAAAGAGAACAAGCAAA TGTAGGTCGAAGAAATATTGCAGAAATGCAGGTACTTGGAGGATATGAAAGAGGAGAAGA TAATAATGAAGAATTAGAGGAAACTGAAAGCTGAAGACAACCAAGAGGCTTATGAGATCT AATGTGAAAATCATCACTCAAGATGCCTCCTGTCAGATGACACATGACGCCAGATAAAAT GTTCAGTGCAATCAGAGTGTACAAATTGTCGTTTTTATTCCTCTTATTGGGATATCATTT TAAAAACTTTATTGGGTTTTTATTGTTGTTGTTTGATCCCTAACCCTACAAAGAGCCTTC CTATTCCCCTCGCTGTTGGAGCAAACCATTATACCTTACTTCCAGCAAGCAAAGTGCTTT GACTTCTTGCTTCAGTCATCAGCCAGCAAGAGGGAACAAAACTGTTCTTTTGCATTTTGC CGCTGAGATATGGCATTGCACTGCTTATATGCCAAGCTAATTTATAGCAAGATATTGATC AAATATAGAAAGTTGATATTCAACCTCACAAGGGCTCTCAAAGTATAATCTTTCTATAGC CAACTGCTAATGCAAATTAAAACATATTTCATTTTAACATGATTTCAAAATCAGTTTTTC ATACTACCCTTTGCTGGAAGAAACTAAAAATATAGCAAATGCAGAACCACAAACAATTCG AATGGGGTAGAAACATTGTAAATATTTACTCTTTGCAAACCCTGGTGGTATTTTATTTTG GCTTCATTTCAATCATTGAAGTATATTCTTATTGGAAATGTACTTTTGGATAAGTAGGGC TAAGCCAGTTGGATCTCTGGTTGTCTAGTCATTGTCATAAGTAAACCTAGTAAAACCTTG TTCTATTTTTCAATCATCAAAAAGTAATTATAAATACGTATTACAAACAAGTGGATGTTT TTAATGACCAATTGAGTAAGAACATCCCTGTCTTAACTGGCCTAAATTTCTTCTGGTAGT GTCAGTTCAACTTTCAGAAGTGCCACTTAAGGAAGTTTGATTTTTGTTTTTGTAATGCAC TGTTTTTAATCTCTCTCTCTTTTTTTTTTTTTTTTTGGTTTTAAAAGCACAATCACTAAA CTTTATTTGTAAACCATTGTAACTATTAACCTTTTTTGTCTTATTGAAAAAAAAAATGTT GAGAAGCGTTTTTAACCTGTTTTGTTAATGCTCTATGTTTGTATTTGGAATATTTGAATA ATGACAGATGGTGACGTAACATGCATACTTTATTGTGGGCCATGAACCAAATGGTTCTTA CTTTTCCTGGACTTAAAGAAAAAAAGAGGTTTAAGTTTGTTGTGGCCAATGTCGAAACCT ACAAGATTTCCTTAAAATCTCTAATAGAGGCATTACTTGCTTTCAATTGACAAATGATGC CCTCTGACTAGTAGATTTCTATGATCCTTTTTTGTCATTTTATGAATATCATTGATTTTA TAATTGGTGCTATTTGAAGAAAAAAATGTACATTTATTCATAGATAGATAAGTATCAGGT CTGACCCCAGTGGAAAACAAAGCCAAACAAAACTGAACCACAAAAAAAAAGGCTGGTGTT CACCAAAACCAAACTTGTTCATTTAGATAATTTGAAAAAGTTCCATAGAAAAGGCGTGCA GTACTAAGGGAACAATCCATGTGATTAATGTTTTCATTATGTTCATGTAAGAAGCCCCTT ATTTTTAGCCATAATTTTGCATACTGAAAATCCAATAATCAGAAAAGTAATTTTGTCACA TTATTTATTAAAAATGTTCTCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_133631 unedited
TCAACATATGTATACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCAAGACCGTGG AGGCAGGAAACGGCACTACTGCGCTTCTGCCTCGGCTCTTTGTTGTTCGCTTTGGATGGT TCTTGAAAGTGTCTGAGCCTCCTCGGAAATCCTGGGGCCGGAGAAGACAAACCTTGGAAT TCTTCCTCTGCAAAAGTCTCTGAGATACTGACAAGCGTCCGGAAAGGTCGACGAGTAATT GCCCTGAAAACTCTTGGCTAATTGACCCACGTTGCTTATATTAAGCCTTTGTGTGTGGTG TGTGGCTTCATACATTTGGGGACCCTATTTCCACTCCCTCCTCTTGGCATGAGACTGTAT ACAGGATCCACCCGAGGACAATGATTGCGGAGCCCGCTCACTTTTACCTGTTTGGATTAA TATGTCTCTGTTCAGGCTCCCGTCTTCGTCAGGAAGATTTTCCACCTCGCATTGTTGAAC ACCCTTCAGACCTGATTGTCTCAAAAGGAGAACCTGCAACTTTGAACTGCAAAGCTGAAG GCCGCCCCACACCCACTATTGAATGGTACAAAGGGGGAGAGAGAGTGGAGACAGACAAAG ATGACCCTCGCTCACACCGAATGTTGCTGCCGAGTGGATCTTTATTTTTCTTACGTATAG TACATGGACGGAAAAGTAGACCTGATGAAGGAGTCTATGTCTGTGTAGCAAGGAATTACC TTGGAGAGGCTGTGAGCCACAATGCATCGCTGGAAGTAGCCATACTTCGGGATGACTTCA GACAAAACCCTTCGGATGTCATGGTTGCAGTANGAGAGCCTGCAGTAATGGAATGCCAAC CTCNCACGAGCCATCCTGAGCACACCATTTCATGGAAGAAAGATGGCTCTCCACTGGGAT GATAAGATGAAAGAAATACTATACGAGGAGGGAAGCTCATGATCACTTACACCCCGTAAA ATGACGCTGCN >OriGene 3' read for NM_133631 unedited
NCCATCCTGNACCGCGCCGCATTCTANGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTT TTATTTGAGAACATTTTTAATAAATAATGTGACAAATTACTTTTCTGATTATTGGATTTT CAGTATGCAAAATTATGGCTAAAAATAAGGGGCTTCTTACATGAACATAATGAAAACATT AATCACATGGATTGTTCCCTTAGTACTGCACGCCTTTTCTATGGAACTTTTTCAAATTAT CTAAATGAACAAGTTTGGTTTTGGTGAACACCAGCCTTTTTTTTTGTGGTTCAGTTTTGT TTGGCTTTGTTTTCCACTGGGGTCAGACCTGATACTTATCTATCTATGAATAAATGTACA TTTTTTTCTTCAAATAGCACCAATTATAAAATCAATGATATTCATAAAATGACAAAAAAG GATCATAGAAATCTACTAGTCAGAGGGCATCATTTGTCAATTGAAAGCAAGTAATGCCTC TATTAGAGATTTTAAGGAAATCTTGTAGGTTTCGACATTGGCCACAACAAACTTAAACCT CTTTTTTTCTTTAAGTCCAGGAAAAGTAAGAACCATTTGGTTCATGGCCCACAATAAAGT ATGCATGTTACGTCACCATCTGTCATTATTCAAATATTCCAAATACAAACATAGAGCATT AACAAAACAGGTTAAAAACGCTTCTCAACATTTTTTTTTTCATTAAGACAAAAAAGGTTA ATAGTTACAATTGGTTTACAAATAAAGTTTATGGAATGTGCTTTTAAAACCCAAAAAAAA AAAAAAAGGAGAGAGAGTTGAAAAACCGTGCTTTTACAAAAACAANAATCAAAACTTCCT TAATGGGCACCTTCTGAAAGTTGAACCGACCCTACCCGAAGAAAATTAGGGCCGTTAAAG ACAAGGGATGTTCTTACTCAATTGGGGCTTAAAAAAAC |
Restriction Sites | NotI-NotI |
ACCN | NM_133631 |
Insert Size | 7300 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_133631.1, NP_598334.1 |
RefSeq Size | 7475 bp |
RefSeq ORF | 4839 bp |
Locus ID | 6091 |
UniProt ID | Q9Y6N7 |
Domains | ig, FN3 |
Protein Families | Druggable Genome |
Protein Pathways | Axon guidance |
Gene Summary | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR, uses a distinct translation start site, includes an alternate in-frame exon in the 5' coding region, and lacks an alternate in-frame exon in the central coding region, compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus, compared to isoform a. The 5' UTR of this variant contains a 55 aa uORF with a strong Kozak signal that may inhibit translation of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Loss of Git2 induces epithelial–mesenchymal transition by miR146a-Cnot6L-controlled expression of Zeb1
,Wu Zhou and Jean Paul Thiery,
J. Cell Sci., Jun 2013; 126: 2740 - 2746.
,PubMed ID 23591815
[ROBO1]
|
Slit-Robo signaling induces malignant transformation through Hakai-mediated E-cadherin degradation during colorectal epithelial cell carcinogenesis
,Wei-Jie Zhou, Zhen H Geng, Shan Chi, Wenli Zhang, Xiao-Feng Niu, Shu-Jue Lan, Li Ma, Xuesong Yang, Li-Jing Wang, Yan-Qing Ding, et al.,
Cell Research (1 February 2011) doi:10.1038/cr.2011.17 Original Article
[Robo1]
|
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211961 | ROBO1 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2 |
CNY 16,576.00 |
|
RC211961L1 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2, Myc-DDK-tagged |
CNY 18,976.00 |
|
RC211961L2 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2, mGFP tagged |
CNY 18,050.00 |
|
RC211961L3 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2, Myc-DDK-tagged |
CNY 18,050.00 |
|
RC211961L4 | Lenti ORF clone of Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2, mGFP tagged |
CNY 18,976.00 |
|
RG211961 | ROBO1 (tGFP-tagged) - Human roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant 2 |
CNY 18,176.00 |