TTC10 (IFT88) (NM_006531) Human Untagged Clone
CAT#: SC109922
IFT88 (untagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2
CNY 6,040.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | D13S1056E; DAF19; hTg737; TG737; TTC10 |
Vector | pCMV6-XL4 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC109922 sequence for NM_006531 edited (data generated by NextGen Sequencing)
ATGATGCAAAATGTGCACCTGGCTCCAGAGACAGATGAAGATGATCTTTATTCCGGCTAT AATGACTACAATCCAATCTATGATATCGAGGAATTGGAGAATGATGCAGCTTTTCAGCAA GCTGTGAGGACTAGTCATGGCAGAAKACCTCCAATAACTGCTAAAATATCAAGCACGGCA GTTACTAGACCTATAGCTACTGGATATGGGTCCAAGACATCTCTGGCATCATCAATAGGA AGACCAATGACAGGGGCTATTCAGGATGGAGTTACTAGACCCATGACAGCAGTGAGAGCA GCTGGTTTTACCAAAGCAGCTTTGAGAGGCTCTGCATTTGACCCCCTTAGTCAGTCAAGG GGCCCTGCTTCCCCTTTGGAAGCCAAGAAAAAAGATAGCCCAGAGGAAAAAATAAAGCAA TTAGAGAAGGAAGTAAATGAGTTGGTAGAAGAAAGCTGTATTGCCAATAGTTGTGGAGAC TTAAAATTGGCCTTAGAAAAGGCAAAAGATGCAGGAAGAAAAGAGAGAGTCCTGGTGAGA CAGCGAGAACAAGTTACAACTCCAGAAAATATCAATTTGGATTTAACTTACTCAGTTCTT TTCAATTTGGCCAGTCAGTATTCAGTTAATGAAATGTATGCCGAAGCACTTAACACTTAT CAAGTTATAGTCAAAAATAAGATGTTTAGCAATGCAGGAATATTGAAAATGAATATGGGA AATATCTATTTAAAGCAAAGAAATTATTCCAAAGCCATTAAATTCTACCGAATGGCATTA GACYAAGTTCCAAGTGTCAATAAGCAAATGAGGATTAAAATAATGCAGAATATTGGAGTT ACATTTATTCAGGCTGGTCAGTATTCAGATGCTATTAATTCATATGAGCACATAATGAGC ATGGCACCAAATCTGAAGGCAGGCTACAACCTAACTATCTGTTATTTTGCTATTGGAGAC CGAGAAAAAATGAAGAAGGCATTCCAAAAATTGATTACTGTTCCATTAGAAATTGATGAA GATAAATATATTTCACCAAGTGATGATCCTCATACTAACTTAGTAACTGAAGCTATAAAA AATGATCACCTCAGGCAAATGGAACGTGAAAGGAAAGCCATGGCAGAAAAATATATTATG ACATCTGCAAAACTCATTGCTCCTGTAATTGAAACATCTTTTGCTGCAGGTTATGATTGG TGCGTGGAAGTGGTGAAAGCTTCTCAATATGTAGAGCTAGCCAATGATCTGGAAATAAAC AAAGCAGTTACATACTTGAGACAAAAAGACTATAACCAAGCTGTAGAGATCTTAAAAGTG TTGGAAAAAAAGGACARTAGAGTGAAAAGTGCAGCTGCAACCAATCTCTCAGCCCTGTAT TATATGGGAAAGGATTTTGCACAAGCCAGCAGCTATGCAGATATAGCTGTGAACTCTGAT AGATATAATCCAGCAGCTCTTACTAATAAAGGGAATACAGTTTTTGCAAATGGTGATTAT GAGAAGGCCGCTGAATTCTATAAAGAGGCTCTAAGAAATGATTCTTCTTGTACTGAAGCA CTTTATAATATTGGCCTTACCTATGAGAAACTAAATCGGCTAGATGAGGCTTTGGACTGT TTCCTGAAACTTCACGCAATCCTACGAAACAGTGCCGAAGTTCTTTACCAGATAGCAAAT ATATATGAATTAATGGAAAATCCCAGTCAAGCTATTGAATGGCTAATGCAGGTGGTCAGT GTTATTCCAACCGATCCTCAAGTTTTATCTAAGCTAGGAGAATTATATGATCGTGAAGGA GATAAATCTCAAGCATTTCAATATTACTATGAGTCATATAGGTATTTTCCTTGTAATATT GAAGTCATTGAGTGGCTTGGAGCCTATTACATTGACACCCAATTTTGGGAAAAAGCTATT CAGTACTTTGAAAGAGCTTCTCTTATACAGCCTACACAAGTGAAATGGCAGCTGATGGTA GCTAGTTGTTTCAGAAGAAGTGGTAACTACCAAAAAGCATTAGATACTTACAAAGATACT CACAGAAAATTTCCAGAAAATGTCGAATGTCTGCGTTTCTTAGTTCGTCTCTGCACAGAT CTTGGATTAAAAGATGCTCAAGAATATGCCAGAAAACTGAAGAGGTTGGAAAAAATGAAA GAAATAAGGGAACAGCGCATAAAGTCAGGCAGAGATGGCAGTGGGGGCTCCCGTGGCAAA AGAGAAGGAAGTGCTAGCGGTGATAGTGGCCAGAACTATAGTGCCAGTAGTAAAGGTGAA CGACTAAGTGCCAGACTCAGAGCTTTACCTGGGACAAATGAACCTTATGAAAGTAGCAGT AACAAAGAAATAGATGCCTCCTATGTGGACCCACTTGGCCCTCAAATAGAACGACCAAAA ACTGCAGCCAAGAAAAGGATCGATGAGGATGATTTTGCTGATGAAGAATTAGGAGATGAT TTGCTTCCAGAATAA Clone variation with respect to NM_006531.3 146 g=>k;784 c=>y;1337 g=>r >OriGene 5' read for NM_006531 unedited
CGCGAATTCGGCACGAGGTCGTCCCTGGGCCCGAATAACTGTCGCCCGCTTCCCTCAGCG TGAGGTGAAGCACTTCAGATTGCCTGATGAAAGTTGAATCAGGAATGAAGATAAAAGTAC ACAACAATGCAGAAAGCGGTACTTAGCCTCACAAAGATGGAAACATTCCAAAAACGTGAA AAACTGCAGTAGGCTTCTTGGAAGTAGACCAAGGGTGTCCAATCTTTGGCTTCCCTGGGC CACATCGGAAGAAGAATTGTCTTGGGCCACACATGAAATTCACAAACACTAAGGTACAAA TGATGCAAAATGTGCACCTGGCTCCAGAGACAGATGAAGATGATCTTTATTCCGGCTATA ATGACTACAATCCAATCTATGATATCGAGGAATTGGAGAATGATGCAGCTTTTCAGCAAG CTGTGAGGACTAGTCATGGCAGAAGACCTCCAATAACTGCTAAAATATCAAGCACGGCAG TTACTAGACCTATAGCTACTGGATATGGGTCCAAGACATCTCTGGCATCATCAATAGGAA GACCAATGACAGGGGCTATTCAGGATGGAGTTACTAGACCCATGACAGCAGTGAGAGCAG CTGGTTTTACCAAAGCAGCTTTGAGAGGCTCTGCATTTGACCCCCTTAGTCAGTCAAGGG GCCCTGCTTTCCCTTTGGAAGCCAAGAANAAAGATAGCCCAGAGGAAAAAATAAAGCATT TAGAGAAGGAAGTAAATGAGTTGGTAGAAGAA |
Restriction Sites | NotI-NotI |
ACCN | NM_006531 |
Insert Size | 3080 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_006531.2, NP_006522.2 |
RefSeq Size | 2872 bp |
RefSeq ORF | 2475 bp |
Locus ID | 8100 |
UniProt ID | Q13099 |
Domains | TPR |
Gene Summary | This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2017] Transcript Variant: This variant (2) lacks multiple alternate exons in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Variants 2 and 9 both encode the same isoform (2). |
Documents
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214282 | IFT88 (Myc-DDK-tagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2 |
CNY 5,800.00 |
|
RC214282L3 | Lenti-ORF clone of IFT88 (Myc-DDK-tagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2 |
CNY 7,700.00 |
|
RC214282L4 | Lenti-ORF clone of IFT88 (mGFP-tagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2 |
CNY 7,700.00 |
|
RG214282 | IFT88 (tGFP-tagged) - Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2 |
CNY 6,370.00 |