TTC10 (IFT88) (NM_006531) Human Untagged Clone

CAT#: SC109922

IFT88 (untagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 2



  "NM_006531" in other vectors (4)

CNY 6,040.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


IFT88 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms D13S1056E; DAF19; hTg737; TG737; TTC10
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC109922 sequence for NM_006531 edited (data generated by NextGen Sequencing)
ATGATGCAAAATGTGCACCTGGCTCCAGAGACAGATGAAGATGATCTTTATTCCGGCTAT
AATGACTACAATCCAATCTATGATATCGAGGAATTGGAGAATGATGCAGCTTTTCAGCAA
GCTGTGAGGACTAGTCATGGCAGAAKACCTCCAATAACTGCTAAAATATCAAGCACGGCA
GTTACTAGACCTATAGCTACTGGATATGGGTCCAAGACATCTCTGGCATCATCAATAGGA
AGACCAATGACAGGGGCTATTCAGGATGGAGTTACTAGACCCATGACAGCAGTGAGAGCA
GCTGGTTTTACCAAAGCAGCTTTGAGAGGCTCTGCATTTGACCCCCTTAGTCAGTCAAGG
GGCCCTGCTTCCCCTTTGGAAGCCAAGAAAAAAGATAGCCCAGAGGAAAAAATAAAGCAA
TTAGAGAAGGAAGTAAATGAGTTGGTAGAAGAAAGCTGTATTGCCAATAGTTGTGGAGAC
TTAAAATTGGCCTTAGAAAAGGCAAAAGATGCAGGAAGAAAAGAGAGAGTCCTGGTGAGA
CAGCGAGAACAAGTTACAACTCCAGAAAATATCAATTTGGATTTAACTTACTCAGTTCTT
TTCAATTTGGCCAGTCAGTATTCAGTTAATGAAATGTATGCCGAAGCACTTAACACTTAT
CAAGTTATAGTCAAAAATAAGATGTTTAGCAATGCAGGAATATTGAAAATGAATATGGGA
AATATCTATTTAAAGCAAAGAAATTATTCCAAAGCCATTAAATTCTACCGAATGGCATTA
GACYAAGTTCCAAGTGTCAATAAGCAAATGAGGATTAAAATAATGCAGAATATTGGAGTT
ACATTTATTCAGGCTGGTCAGTATTCAGATGCTATTAATTCATATGAGCACATAATGAGC
ATGGCACCAAATCTGAAGGCAGGCTACAACCTAACTATCTGTTATTTTGCTATTGGAGAC
CGAGAAAAAATGAAGAAGGCATTCCAAAAATTGATTACTGTTCCATTAGAAATTGATGAA
GATAAATATATTTCACCAAGTGATGATCCTCATACTAACTTAGTAACTGAAGCTATAAAA
AATGATCACCTCAGGCAAATGGAACGTGAAAGGAAAGCCATGGCAGAAAAATATATTATG
ACATCTGCAAAACTCATTGCTCCTGTAATTGAAACATCTTTTGCTGCAGGTTATGATTGG
TGCGTGGAAGTGGTGAAAGCTTCTCAATATGTAGAGCTAGCCAATGATCTGGAAATAAAC
AAAGCAGTTACATACTTGAGACAAAAAGACTATAACCAAGCTGTAGAGATCTTAAAAGTG
TTGGAAAAAAAGGACARTAGAGTGAAAAGTGCAGCTGCAACCAATCTCTCAGCCCTGTAT
TATATGGGAAAGGATTTTGCACAAGCCAGCAGCTATGCAGATATAGCTGTGAACTCTGAT
AGATATAATCCAGCAGCTCTTACTAATAAAGGGAATACAGTTTTTGCAAATGGTGATTAT
GAGAAGGCCGCTGAATTCTATAAAGAGGCTCTAAGAAATGATTCTTCTTGTACTGAAGCA
CTTTATAATATTGGCCTTACCTATGAGAAACTAAATCGGCTAGATGAGGCTTTGGACTGT
TTCCTGAAACTTCACGCAATCCTACGAAACAGTGCCGAAGTTCTTTACCAGATAGCAAAT
ATATATGAATTAATGGAAAATCCCAGTCAAGCTATTGAATGGCTAATGCAGGTGGTCAGT
GTTATTCCAACCGATCCTCAAGTTTTATCTAAGCTAGGAGAATTATATGATCGTGAAGGA
GATAAATCTCAAGCATTTCAATATTACTATGAGTCATATAGGTATTTTCCTTGTAATATT
GAAGTCATTGAGTGGCTTGGAGCCTATTACATTGACACCCAATTTTGGGAAAAAGCTATT
CAGTACTTTGAAAGAGCTTCTCTTATACAGCCTACACAAGTGAAATGGCAGCTGATGGTA
GCTAGTTGTTTCAGAAGAAGTGGTAACTACCAAAAAGCATTAGATACTTACAAAGATACT
CACAGAAAATTTCCAGAAAATGTCGAATGTCTGCGTTTCTTAGTTCGTCTCTGCACAGAT
CTTGGATTAAAAGATGCTCAAGAATATGCCAGAAAACTGAAGAGGTTGGAAAAAATGAAA
GAAATAAGGGAACAGCGCATAAAGTCAGGCAGAGATGGCAGTGGGGGCTCCCGTGGCAAA
AGAGAAGGAAGTGCTAGCGGTGATAGTGGCCAGAACTATAGTGCCAGTAGTAAAGGTGAA
CGACTAAGTGCCAGACTCAGAGCTTTACCTGGGACAAATGAACCTTATGAAAGTAGCAGT
AACAAAGAAATAGATGCCTCCTATGTGGACCCACTTGGCCCTCAAATAGAACGACCAAAA
ACTGCAGCCAAGAAAAGGATCGATGAGGATGATTTTGCTGATGAAGAATTAGGAGATGAT
TTGCTTCCAGAATAA

Clone variation with respect to NM_006531.3
146 g=>k;784 c=>y;1337 g=>r
>OriGene 5' read for NM_006531 unedited
CGCGAATTCGGCACGAGGTCGTCCCTGGGCCCGAATAACTGTCGCCCGCTTCCCTCAGCG
TGAGGTGAAGCACTTCAGATTGCCTGATGAAAGTTGAATCAGGAATGAAGATAAAAGTAC
ACAACAATGCAGAAAGCGGTACTTAGCCTCACAAAGATGGAAACATTCCAAAAACGTGAA
AAACTGCAGTAGGCTTCTTGGAAGTAGACCAAGGGTGTCCAATCTTTGGCTTCCCTGGGC
CACATCGGAAGAAGAATTGTCTTGGGCCACACATGAAATTCACAAACACTAAGGTACAAA
TGATGCAAAATGTGCACCTGGCTCCAGAGACAGATGAAGATGATCTTTATTCCGGCTATA
ATGACTACAATCCAATCTATGATATCGAGGAATTGGAGAATGATGCAGCTTTTCAGCAAG
CTGTGAGGACTAGTCATGGCAGAAGACCTCCAATAACTGCTAAAATATCAAGCACGGCAG
TTACTAGACCTATAGCTACTGGATATGGGTCCAAGACATCTCTGGCATCATCAATAGGAA
GACCAATGACAGGGGCTATTCAGGATGGAGTTACTAGACCCATGACAGCAGTGAGAGCAG
CTGGTTTTACCAAAGCAGCTTTGAGAGGCTCTGCATTTGACCCCCTTAGTCAGTCAAGGG
GCCCTGCTTTCCCTTTGGAAGCCAAGAANAAAGATAGCCCAGAGGAAAAAATAAAGCATT
TAGAGAAGGAAGTAAATGAGTTGGTAGAAGAA
Restriction Sites NotI-NotI     
ACCN NM_006531
Insert Size 3080 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_006531.2, NP_006522.2
RefSeq Size 2872 bp
RefSeq ORF 2475 bp
Locus ID 8100
UniProt ID Q13099
Domains TPR
Gene Summary This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2017]
Transcript Variant: This variant (2) lacks multiple alternate exons in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Variants 2 and 9 both encode the same isoform (2).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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