Bim (BCL2L11) (NM_138626) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BAM; BIM; BOD
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_138626 edited GAATTCGGCACGAGGTCTGCGTCCAGCGCCGCTGCCGCTGCCGCTGCCGCCGCCGCCGCC GCCGCCGCCACTACCACCACTTGATTCTTGCAGCCACCCTGCGAACCCTGCCACACTGCG ATCGCATCATCGCGGTATTCGGTTCGCTGCGTTCCCGCCGCCACCGCCTCGGCGCCCTTT CTTGGCCCTTGTTCCCCCAAATGTCTGACTCTGACTCTCGGACTGAGAAACGCAAGAAAA AAAGACCAAATGGCAAAGCAACCTTCTGATGTAAGTTCTGAGTGTGACCGAGAAGGTAGA CAATTGCAGCCTGCGGAGAGGCCTCCCCAGCTCAGACCTGGGGCCCCTACCTCCCTACAG ACAGAGCCACAAGGTAATCCTGAAGGCAATCACGGAGGTGAAGGGGACAGCTGCCCCCAC GGCAGCCCTCAGGGCCCGCTGGCCCCACCTGCCAGCCCTGGCCCTTTTGCTACCAGATCC CCGCTTTTCATCTTTATGAGAAGATCCTCCCTGCTGTCTCGATCCTCCAGTGGGTATTTC TCTTTTGACACAGACAGGAGCCCAGCACCCATGAGTTGTGACAAATCAACACAAACCCCA AGTCCTCCTTGCCAGGCCTTCAACCACTATCTCAGTGCAATGGGTATTTTTGAATAATTA CCAAGCAGCCGAAGACCACCCACGAATGGTTATCTTACGACTGTTACGTTACATTGTCCG CCTGGTGTGGAGAATGCATTGACAGGTTCTTTGCGGAGCCGAGATACCATGCAGACATTT TGCTTGTTCAAACCAACAAGACCCAGCACCGCGGTCTCCTGGTGCCATTATTATGCAGCC AGCGGTTCTCTTGTGGAGGGGGCAGGTGACGTTTCAGAAGACACCGAGCTGGATGGGACT ACCTTTCTGTTCATCACCACACAGCAGAATTTCTAATGGAAGTTTGTTGTGAATGTAAAG GAGGGAGCATTCTTTGCTTTTTAATATACAAACCATGGTTTTTTGGAGCAGGATTTTGTG TAAGAATGGTGTTTACATGCAGTGTGTTTTCCCCCTCACCTTCAATAAGGTTTTTCAAAA AGGAAATGGAAACTTTTTAACCAATTTGTGAATAACTTTTGTATTAAAATTTTAAGAACC TACGGCCTATTCTCAGAGGATTATGTAACCCCTGCAGTGGAAACTGAGCCAGCTAACTTA AAAAGCTGCCTTAGTTTATTTTTAGAGATTACAGAATTTTTAAACAGGGAGACGTGTGAT ATACTCCCTCCCTTCCCTACTATTGCCTCTCTGACCTTTTTAAATTATTTTTAATACCAA AAGAGTTCTTTTGAAATGGAACTGATTAAAAGGGCAGAGGGTCTGTTGCCAGCCTGCATT GATATACCAGTCCCATTTGTAAATATTTACGTACCTTTATAAATTCAGTTGCATCTGTGG CAAAATTTCAGACTATTTTTGCGTCTTTCCTCATCACTTTTTGTGATGCAACTCCAGTCT GGACTCAGATGCATAGATTTGGTCCAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCTCG AC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_138626 unedited CCCGCCATTACCCCCGCCCGTTGACGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC GGCCGCGAATTCGGCACGAGGTCTGCGTCCAGCGCCGCTGCCGCTGCCGCTGCCGCCGCC GCCGCCGCCGCCGCCACTACCACCACTTGATTCTTGCAGCCACCCTGCGAACCCTGCCAC ACTGCGATCGCATCATCGCGGTATTCGGTTCGCTGCGTTCCCGCCGCCACCGCCTCGGCG CCCTTTCTTGGCCCTTGTTCCCCCAAATGTCTGACTCTGACTCTCGGACTGAGAAACGCA AGAAAAAAAGACCAAATGGCAAAGCAACCTTCTGATGTAAGTTCTGAGTGTGACCGAGAA GGTAGACAATTGCAGCCTGCGGAGAGGCCTCCCCAGCTCAGACCTGGGGCCCCTACCTCC CTACAGACAGAGCCACAAGGTAATCCTGAAGGCAATCACGGAGGTGAAGGGGACAGCTGC CCCCACGGCAGCCCTCAGGGCCCGCTGGCCCCACCTGCCAGCCCTGGCCCTTTTGCTACC AGACCCTCGCTTTTCATCTTTATGAGAAGAGCCTCCCTGCTGTCTCGATCCTCCAGTGGG TATTTCTCTTTTGACACAGACAGGAGCCCAGCACCCATGAGTTGTGACANATCAACACAA ACCCCAAGTCCTCCTTGCCAGGCCTTCAACCACTATCTCAGTGCAATGGGTATTTTTTGA ATAATTACCAAGCAGCCGAAGACCACCCACGAATGGTTATCTTACGACTGTTACGTTACA TTGTCCGCCCTGTGGTGGAGAATGCATTGACAGGGATCTTTGCNGAGCCGAGAACCATGC AGACATTNTGCTTGNTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_138626 unedited CGGTATTACTATGGNACCGCGGCCGCAATTCTANATCGAGTTTTTTTTTTTTTTTTTTAC TTATTTATGCTGTACAAAATTTTACAAGGTAAAGGAAACACAATTGCACACAGACATTTG GCTCAGTTGATAAGTAATATGACCTACGAATCTAATAAATACTCACAATATATACATTAA GAAGGGGAAACGGCAGACAATTGTATTCCACTATATTTTTTTATTTAGAAGGAATTTTAA ACTTGATTTGGGCGCAACCCCCACCCCCAAAATTCTTGATCAACTAACCAGTTGGGAAGG GCATCTGAAGAATCACTCATGAAACTATTTTGTGTGTGTTTATTGCGTTTCTGTGTTAAG TCACAGGACACTTTTTACAAACCTCACATAGGGAGAGGTTGTCAAGGCACAAAACCTGCA GTAAGTTTCCAAAAAGGGGATTTAATTATTTTTTCTACAAAAATGTTTTTTAAAAAAGGT AAAAACGTATACCCACGGGAGGCATACTTTCTGCACAGAGGCATTTTGATAACCCGATAC ATACAGTACAGAGACATCCAGTCATCGCTTTAAGTTCTTGTGCCCACAGCCCGGGCTTTA ATACTCCCACAATTCATAGAAATTTTAATTTTCTCAGGAACTGGTTCCACACCAGCCTGG AGGGCGCCAGGGCACAAGGGAATGTGCTTATGTGGGGCACACAACCCCTGGGGGAAAAAA GGGAAGGGCCTCCCTTGCCCGGGCCAGGGTTGGCCCCAAGTGTGCCCCCAGGGCTTCAAG GGTGGGGAAAAAAAATAAAAGGCTTTTTTTCGGGAGACCTGGCCCGCGATACCGGGGTTC CTGTGGGGGGGGGCGCCAAACCAAAAACATTTTGGGTGATGTTGTTTCCCACCAGC
Restriction Sites	NotI-NotI
ACCN	NM_138626
Insert Size	4000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_138626.2, NP_619532.1
RefSeq Size	3318 bp
RefSeq ORF	408 bp
Locus ID	10018
UniProt ID	O43521
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family and to act as an apoptotic activator. The expression of this gene can be induced by nerve growth factor (NGF), as well as by the forkhead transcription factor FKHR-L1, which suggests a role of this gene in neuronal and lymphocyte apoptosis. Transgenic studies of the mouse counterpart suggested that this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants of this gene have been identified. [provided by RefSeq, Jun 2013] Transcript Variant: This variant (7, also known as Bim-ABC and Bim-beta2) lacks an exon in the 3' coding region which results in a frameshift, compared to variant 1. The resulting isoform (7) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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