CRF1 (CRHR1) (NM_004382) Human Untagged Clone

CAT#: SC110860

CRHR1 (untagged)-Human corticotropin releasing hormone receptor 1 (CRHR1), transcript variant 2



  "NM_004382" in other vectors (6)

CNY 3,656.00

CNY 6,650.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-CRHR1 Antibody (N-Terminus)
    • 50 ug

CNY 5,654.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CRF-R; CRF-R-1; CRF-R1; CRF1; CRFR-1; CRFR1; CRH-R-1; CRH-R1; CRHR; CRHR1L
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>SC110860 representing NM_004382.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

ATGGGAGGGCACCCGCAGCTCCGTCTCGTCAAGGCCCTTCTCCTTCTGGGGCTGAACCCCGTCTCTGCC
TCCCTCCAGGACCAGCACTGCGAGAGCCTGTCCCTGGCCAGCAACATCTCAGGACTGCAGTGCAACGCA
TCCGTGGACCTCATTGGCACCTGCTGGCCCCGCAGCCCTGCGGGGCAGCTAGTGGTTCGGCCCTGCCCT
GCCTTTTTCTATGGTGTCCGCTACAATACCACAAACAATGGCTACCGGGAGTGCCTGGCCAATGGCAGC
TGGGCCGCCCGCGTGAATTACTCCGAGTGCCAGGAGATCCTCAATGAGGAGAAAAAAAGCAAGGTGCAC
TACCATGTCGCAGTCATCATCAACTACCTGGGCCACTGTATCTCCCTGGTGGCCCTCCTGGTGGCCTTT
GTCCTCTTTCTGCGGCTCAGGAGCATCCGGTGCCTGCGAAACATCATCCACTGGAACCTCATCTCCGCC
TTCATCCTGCGCAACGCCACCTGGTTCGTGGTCCAGCTAACCATGAGCCCCGAGGTCCACCAGAGCAAC
GTGGGCTGGTGCAGGTTGGTGACAGCCGCCTACAACTACTTCCATGTGACCAACTTCTTCTGGATGTTC
GGCGAGGGCTGCTACCTGCACACAGCCATCGTGCTCACCTACTCCACTGACCGGCTGCGCAAATGGATG
TTCATCTGCATTGGCTGGGGTGTGCCCTTCCCCATCATTGTGGCCTGGGCCATTGGGAAGCTGTACTAC
GACAATGAGAAGTGCTGGTTTGGCAAAAGGCCTGGGGTGTACACCGACTACATCTACCAGGGCCCCATG
ATCCTGGTCCTGCTGATCAATTTCATCTTCCTTTTCAACATCGTCCGCATCCTCATGACCAAGCTCCGG
GCATCCACCACGTCTGAGACCATTCAGTACAGGAAGGCTGTGAAAGCCACTCTGGTGCTGCTGCCCCTC
CTGGGCATCACCTACATGCTGTTCTTCGTCAATCCCGGGGAGGATGAGGTCTCCCGGGTCGTCTTCATC
TACTTCAACTCCTTCCTGGAATCCTTCCAGGGCTTCTTTGTGTCTGTGTTCTACTGTTTCCTCAATAGT
GAGGTCCGTTCTGCCATCCGGAAGAGGTGGCACCGGTGGCAGGACAAGCACTCGATCCGTGCCCGAGTG
GCCCGTGCCATGTCCATCCCCACCTCCCCAACCCGTGTCAGCTTTCACAGCATCAAGCAGTCCACAGCA
GTCTGA

>OriGene 5' read for NM_004382 unedited
NGGTCAAAATTTGTATACGACTCATATAGGCGGCNCGCGATTCGGCACGAGGGAGAGCCG
GGCCGGGCCGGGCCGGGCCGCGGGGCCGGGAAGCGCCGAGCCGGGCATCTCCTCACCAGG
CAGCGACCGAGGAGCCCGGCCGCCCACCCCGTGCCGCCCGAGCCCGCAGCCGCCCGCCGG
TCCCTCTGGGATGTCCGTAGGACCCGGGCATTCAGGACGGTAGCCGAGCGAGCCCGAGGA
TGGGAGGGCACCCGCAGCTCCGTCTCGTCAAGGCCCTTCTCCTTCTGGGGCTGAACCCCG
TCTCTGCCTCCCTCCAGGACCAGCACTGCGAGAGCCTGTCCCTGGCCAGCAACATCTCAG
GACTGCAGTGCAACGCATCCGTGGACCTCATTGGCACCTGCTGGCCCCGCAGCCCTGCGG
GGCAGCTAGTGGTTCGGCCCTGCCCTGCCTTTTTCTATGGTGTCCGCTACAATACCACAA
ACAATGGCTACCGGGAGTGCCTGGCCAATGGCAGCTGGGCCGCCCGCGTGAATTACTCCG
AGTGCCAGGAGATCCTCAATGAGGAGAAAAAAAGCAAGGTGCACTACCATGTCGCAGTCA
TCATCAACTACCTGGGCCACTGTATCTCCCTGGTGGCCCTCCTGGTGGCCTTTGTCCTCT
TTCTGCGGCTCAGGAGCATCCGGTGCCTGCGAAACATCATCCACTGGAACCTCATCTCCG
CCTTCATCCTGCGCAACGCCACCTGGTTTCGTGGTCCAGCTAACCATGACCCCCCGAGGT
CCACCAGACCAACGTGGGCTGGTGGCCAGGGTTGGNTGACAGCCGCCTACAACTACTTNC
ATGTGACCAACTCTNTTCTGGATGTTCNGGNGAAGGGCTGCTACCTGCACACANNCATCG
TGCTCACCTACTNCACTGACCGCTGCGAAA
>OriGene 3' read for NM_004382 unedited
CCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTGGTTGAAAAGAAAA
TTTTATTTATACCAGCCACCCGCCTCACAGCCGACACCCTCATCTTCTAGTGCCCCCCAA
AGCCCTGCCCTGGCTGTCCAGTCCCTCTGGACATGGGCAGGTCAGTGGGGGCTGCGGCCG
GTCCACACCTGGAGTGTAAGCAGCACGTTGTCCCAAGAGCCACTTGGGCAGGGGTCTTCT
CCTGGCTTGCTTAGCTAGTGGTCCTGCCCCAGAGGCCATCCAGGGCTACAAGCTCTGCCC
CAGAGGCTGGGACTGGGACACCCCTGGCTCTTGCTCACAGGCCACTCTGCCCCCTCCAGC
CCCCATCTTCTCACAAAAGAGGAAAAGGAGCAGGAGGTGACTGGTATGGGGTGGTTAAGT
GAGGGGAAGCTGGCCTGGCCTGCAGGGTACTAAATGTTCAGGGTGAAGGCAGCAAGGCAG
GGCATTGCTGGTGGCAGTGCCACAGTGCCAGTAAGGTTCTGGAGGCCTGGGGGGGTGACT
CTAGTGCTGTGGCCGCAAGTCTGATGATGACACCTGACTTCTGTCTCCAGGGTTCCTGAG
TGAGGGCCCTTGGTTCCCAGTGGTGTCGGAAGGCATCACCGANGTCCAGAGGCGTCATCG
TGGTGAGTCAGAGGCTGTCACGAGTTGCCCATGATGNCCCAGGGCAGCAAATGGCCTCCC
CACGGTTGCCGNAGGCAGCCCCANGGNCCANNTGGGCTGNCCTTCTTGTGCTCTGGGAGA
GACAGCCTTNGAGGACATGCGTGCTGCTGTAAGTGTCCAGCGCCCCATTTCATNCATTCC
ATGTCCCTTCTCAGGAGGATTGGCAAGGAACCNAGCCCTGGCCCGNCCANCCTGTAGGGA
CTTCACTCTGAACTNAGTCTCGCCGGTGCAGGGAAAGGGGCCCCCAGCTGNNATGCATTG
TCTGGGGAGGGGCTGAACCTACAGCACTCCACGCGG
Restriction Sites NotI-NotI     
ACCN NM_004382
Insert Size 1248 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004382.2
RefSeq Size 2536 bp
RefSeq ORF 1248 bp
Locus ID 1394
UniProt ID P34998
Domains 7tm_2, HormR
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Long-term depression, Neuroactive ligand-receptor interaction
MW 47.7 kDa
Gene Summary This gene encodes a G-protein coupled receptor that binds neuropeptides of the corticotropin releasing hormone family that are major regulators of the hypothalamic-pituitary-adrenal pathway. The encoded protein is essential for the activation of signal transduction pathways that regulate diverse physiological processes including stress, reproduction, immune response and obesity. Alternative splicing results in multiple transcript variants. Naturally-occurring readthrough transcription between this gene and upstream GeneID:147081 results in transcripts that encode isoforms that share similarity with the products of this gene. [provided by RefSeq, Aug 2016]
Transcript Variant: This variant (1a, also known as CRHR1alpha), lacks an alternate in-frame exon in the central coding region, compared to variant 1b, resulting in an isoform (2) that is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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