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PIM1 (NM_002648) Human Untagged Clone
CAT＃: SC110975
PIM1 (untagged)-Human pim-1 oncogene (PIM1)
CNY 2,400.00
CNY 2,950.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PIM |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_002648 edited
ATGCTCTTGTCCAAAATCAACTCGCTTGCCCACCTGCGCGCCGCGCCCTGCAACGACCTG CACGCCACCAAGCTGGCGCCCGGCAAGGAGAAGGAGCCCCTGGAGTCGCAGTACCAGGTG GGCCCGCTACTGGGCAGCGGCGGCTTCGGCTCGGTCTACTCAGGCATCCGCGTCTCCGAC AACTTGCCGGTGGCCATCAAACACGTGGAGAAGGACCGGATTTCCGACTGGGGAGAGCTG CCTAATGGCACTCGAGTGCCCATGGAAGTGGTCCTGCTGAAGAAGGTGAGCTCGGGTTTC TCCGGCGTCATTAGGCTCCTGGACTGGTTCGAGAGGCCCGACAGTTTCGTCCTGATCCTG GAGAGGCCCGAGCCGGTGCAAGATCTCTTCGACTTCATCACGGAAAGGGGAGCCCTGCAA GAGGAGCTGGCCCGCAGCTTCTTCTGGCAGGTGCTGGAGGCCGTGCGGCACTGCCACAAC TGCGGGGTGCTCCACCGCGACATCAAGGACGAAAACATCCTTATCGACCTCAATCGCGGC GAGCTCAAGCTCATCGACTTCGGGTCGGGGGCGCTGCTCAAGGACACCGTCTACACGGAC TTCGATGGGACCCGAGTGTATAGCCCTCCAGAGTGGATCCGCTACCATCGCTACCATGGC AGGTCGGCGGCAGTCTGGTCCCTGGGGATCCTGCTGTATGATATGGTGTGTGGAGATATT CCTTTCGAGCATGACGAAGAGATCATCAGGGGCCAGGTTTTCTTCAGGCAGAGGGTCTCT TCAGAATGTCAGCATCTCATTAGATGGTGCTTGGCCCTGAGACCATCAGATAGGCCAACC TTCGAAGAAATCCAGAACCATCCATGGATGCAAGATGTTCTCCTGCCCCAGGAAACTGCT GAGATCCACCTCCACAGCCTGTCGCCGGGGCCCAGCAAATAG >OriGene 5' read for NM_002648 unedited
ATCCATCTCCCACCAGCGGCGGCGGGACCGGCAGCAGCAGCAGCAGCAGCAGCAGCATCC ACTAGCCTCCTGCCCCGCGGCGCTGCCGCACGAGCCCCACGAGCCGCTCACCCCGCCGTT CTCAGCGCTGCCCGACCCCGCTGGCGCGCCCTCCCGCCGCCAGTCCCGGCAGCGCCCCTG CCGTCCTCCGACTCGCCCTCGGCCTTCCGCGCCAGCCGCAGCGACAGCCGCAACGCCACC CGCAGCCACAGCCACAGCCACAGCCCCAGGCATAGCCTTCGGCACAGCCCCGGCTCCGGC TCCTGCGGCAGCTCCTCTGGGCACCGTCCCTGCGCCGACATCCTGGAGGTTGGGATGCTC TTGTCCAAAATCAACTCGCTTGCCCACCTGCGCGCCGCGCCCTGCAACGACCTGCACGCC ACCAAGCTGGCGCCCGGCAAGGAGAAGGAGCCCCTGGAGTCGCAGTACCAGGTGGGCCCG CTACTGGGCAGCGGCGGCTTCGGCTCGGTCTACTCAGGCATCCGCGTCTCCGACAACTTG CCGGTGGCCATCAAACACGTGGAGAAGGACCGGATTTCCGACTGGGGAGAGCTGCCTAAT GGCACTCGAGTGCCCATGGAAGTGGTCCTGCTGAANAAGTGAGCTCGGGTTTCTCCGGCG TCATTAGGCTCCTGGACTGGTTCGAGAGGCCCGACAGNTTCGTCCTGATCCTGGAGAGGC CCGAGCCGGTGCAAGATCTCTTCGACTTCCTCACGGAAAGGGGAGCCCTGCAAGAAGAGC TGGCCCGCAACTTCTTCTGGCAAGTGCTGGNAGCCGTGCGGTACTGCCCAATT >OriGene 3' read for NM_002648 unedited
TTTTTTTTTTTTTTTTTAAAACAAAGGTTTTTATTCAAAAAACGCCAAGTATAAAAAAAA AAATAAGATCTCTTTTATTCCCCTGTACAGTATTTCACTCAAATAAAACCAGCAGGCTAC CTGCTGCTCAAAACACAGCCCCAGAGAGGATCTGGAAGGCACACCATCCAAAACTGCTTT TCATAATTATCCTATATACACAGTCAGCAATGCCCCTCCCCATCACCCACAGAGCCGGGA CCCACCTGGGTAAGTAGGTGAGGGGCCCCACAGAAGCCAGGGAGCTGCAGCACTGTCCCG CCTGAGTCCCGTCACCCCACAAACAGAGCAAGCGTCGCAGCCCTCCCCCAAAAGGTCCCC AAGTCAGAGGAGGAAAGGAGGAAAGAAAACTAGGGCAGCAGCTATGGAAGCTTGGAATAG GAAGGGAACCAAATAAATAAATAAATAAATA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002648 |
| Insert Size | 942 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002648.2, NP_002639.1 |
| RefSeq Size | 2684 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 5292 |
| UniProt ID | P11309 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase |
| Protein Pathways | Acute myeloid leukemia, Jak-STAT signaling pathway |
| Gene Summary | The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011] Transcript Variant: This variant (1) encodes two isoforms resulting from the use of alternate in-frame, translation initiation codons. This RefSeq represents the shorter isoform (2, also known as Pim-1S) derived from the use of a downstream AUG (at nt 431-433). Pim-1S has been shown to localize predominantly in the nucleus (PMID:16186805). |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Discovery of novel triazolo[4,3-b]pyridazin-3-yl-quinoline derivatives as PIM inhibitors
,Martínez-González, S;Rodríguez-Arístegui, S;Gómez de la Oliva, CA;Hernández, AI;González Cantalapiedra, E;Varela, C;García, AB;Rabal, O;Oyarzabal, J;Bischoff, JR;Klett, J;Albarrán, MI;Cebriá, A;Ajenjo, N;García-Serelde, B;Gómez-Casero, E;Cuadrado-Urbano, M;Cebrián, D;Blanco-Aparicio, C;Pastor, J;,
Eur J Med Chem
,PubMed ID 30802730
[PIM1]
|
|
Human CD180 Transmits Signals via the PIM-1L Kinase
,Egli, N;Zajonz, A;Burger, MT;Schweighoffer, T;,
PLoS ONE
,PubMed ID 26555723
[PIM1]
|
|
Phosphorylation of the androgen receptor by PIM1 in hormone refractory prostate cancer
,S Ha, N J Iqbal, P Mita, R Ruoff, W L Gerald, H Lepor, S S Taneja, P Lee, J Melamed, M J Garabedian & S K Logan,
Oncogene doi:10.1038/onc.2012.412
[PIM1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205853 | PIM1 (Myc-DDK-tagged)-Human pim-1 oncogene (PIM1) |
CNY 3,600.00 |
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| RC205853L1 | Lenti ORF clone of Human pim-1 oncogene (PIM1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC205853L2 | Lenti ORF clone of Human pim-1 oncogene (PIM1), mGFP tagged |
CNY 5,890.00 |
|
| RC205853L3 | Lenti ORF clone of Human pim-1 oncogene (PIM1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC205853L4 | Lenti ORF clone of Human pim-1 oncogene (PIM1), mGFP tagged |
CNY 6,000.00 |
|
| RG205853 | PIM1 (tGFP-tagged) - Human pim-1 oncogene (PIM1) |
CNY 5,200.00 |
|
| SC323357 | PIM1 (untagged)-Kinase deficient mutant (K67M) of Human pim-1 oncogene (PIM1) |
CNY 2,400.00 |
