Hsp60 (HSPD1) (NM_002156) Human Untagged Clone

Name: Hsp60 (HSPD1) (NM_002156) Human Untagged Clone
SKU: SC111640
Price: 4272.00

CAT＃: SC111640

HSPD1 (untagged)-Human heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1

Datasheet

SDS

"NM_002156" in other vectors (6)

CNY 4,272.00

货期*

现货

规格

Cited in 2 publications.

Product images

经常一起买 (4)

TA800758

HSPD1 mouse monoclonal antibody,clone OTI3A2

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC111640 sequence for NM_002156 edited (data generated by NextGen Sequencing) ATGCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTACTGGCTCCT CATCTCACTCGGGCTTATGCCAAAGATGTAAAATTTGGTGCAGATGCCCGAGCCTTAATG CTTCAAGGTGTAGACCTTTTAGCCGATGCTGTGGCCGTTACAATGGGGCCAAAGGGAAGA ACAGTGATTATTGAGCAGAGTTGGGGAAGTCCCAAAGTAACAAAAGATGGTGTGACTGTT GCAAAGTCAATTGACTTAAAAGATAAATACAAAAACATTGGAGCTAAACTTGTTCAAGAT GTTGCCAATAACACAAATGAAGAAGCTGGGGATGGCACTACCACTGCTACTGTACTGGCA CGCTCTATAGCCAAGGAAGGCTTCGAGAAGATTAGCAAAGGTGCTAATCCAGTGGAAATC AGGAGAGGTGTGATGTTAGCTGTTGATGCTGTAATTGCTGAACTTAAAAAGCAGTCTAAA CCTGTGACCACCCCTGAAGAAATTGCACAGGTTGCTACGATTTCTGCAAACGGAGACAAA GAAATTGGCAATATCATCTCTGATGCAATGAAAAAAGTTGGAAGAAAGGGTGTCATCACA GTAAAGGATGGAAAAACACTGAATGATGAATTAGAAATTATTGAAGGCATGAAGTTTGAT CGAGGCTATATTTCTCCATACTTTATTAATACATCAAAAGGTCAGAAATGTGAATTCCAG GATGCCTATGTTCTGTTGAGTGAAAAGAAAATTTCTAGTATCCAGTCCATTGTACCTGCT CTTGAAATTGCCAATGCTCACCGTAAGCCTTTGGTCATAATCGCTGAAGATGTTGATGGA GAAGCTCTAAGTACACTCGTCTTGAATAGGCTAAAGGTTGGTCTTCAGGTTGTGGCAGTC AAGGCTCCAGGGTTTGGTGACAATAGAAAGAACCAGCTTAAAGATATGGCTATTGCTACT GGTGGTGCAGTGTTTGGAGAAGAGGGATTGACCCTGAATCTTGAAGACGTTCAGCCTCAT GACTTAGGAAAAGTTGGAGAGGTCATTGTGACCAAAGACGATGCCATGCTCTTAAAAGGA AAAGGTGACAAGGCTCAAATTGAAAAACGTATTCAAGAAATCATTGAGCAGTTAGATGTC ACAACTAGTGAATATGAAAAGGAAAAACTGAATGAACGGCTTGCAAAACTTTCAGATGGA GTGGCTGTGCTGAAGGTTGGTGGGACAAGTGATGTTGAAGTGAATGAAAAGAAAGACAGA GTTACAGATGCCCTTAATGCTACAAGAGCTGCTGTTGAAGAAGGCATTGTTTTGGGAGGG GGTTGTGCCCTCCTTCGATGCATTCCAGCCTTGGACTCATTGACTCCAGCTAATGAAGAT CAAAAAATTGGTATAGAAATTATTAAAAGAACACTCAAAATTCCAGCAATGACCATTGCT AAGAATGCAGGTGTTGAAGGATCTTTGATAGTTGAGAAAATTATGCAAAGTTCCTCAGAA GTTGGTTATGATGCTATGGCTGGAGATTTTGTGAATATGGTGGAAAAAGGAATCATTGAC CCAACAAAGGTTGTGAGAACTGCTTTATTGGATGCTGCTGGTGTGGCCTCTCTGTTAACT ACAGCAGAAGTTGTAGTCACAGAAATTCCTAAAGAAGAGAAGGACCCTGGAATGGGTGCA ATGGGTGGAATGGGAGGTGGTATGGGAGGTGGCATGTTCTAA Clone variation with respect to NM_002156.4 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_002156 unedited TTTAGGTTTTGTAATACGACTTACTATAGGGCGGCCGACGAATTCGCACGAGGGTCTCGC CGAGCGCACGCCTTGCCGCCGCCCCGCAGAATGCTTCGGTTACCCACAGTCTTTCGCCAG ATGAGACCGGTGTCCAGGGTACTGGCTCCTCATCTCACTCGGGCTTATGCCAAAGATGTA AAATTTGGTGCAGATGCCCGAGCCTTAATGCTTCAAGGTGTAGACCTTTTAGCCGATGCT GTGGCCGTTACAATGGGGCCAAAGGGAAGAACAGTGATTATTGAGCAGAGTTGGGGAAGT CCCAAAGTAACAAAAGATGGTGTGACTGTTGCAAAGTCAATTGACTTAAAAGATAAATAC AAAAACATTGGAGCTAAACTTGTTCAAGATGTTGCCAATAACACAAATGAAGAAGCTGGG GATGGCACTACCACTGCTACTGTACTGGCACGCTCTATAGCCAAGGAAGGCTTCGAGAAG ATTAGCAAAGGTGCTAATCCAGTGGAAATCAGGAGAGGTGTGATGTTAGCTGTTGATGCT GTAATTGCTGAACTTAAAAAGCAGTCTAAACCTGTGACCACCCCTGAAGAAATTGCACAG GTTGCTACGATTTCTGCAAACGGAGACCAAGAAATTGGCAATATCATCTCTGATGCAATG AANAAAGTTGGAAGAAAGGGTGTCATCACAGTAAAGGATGGAAAAACACTGAATGATGAA TTAGAAATTATTGAAGGCATNGAGTTTGATCGAGGCTATNATTCCCATACTTTATTAATA CATCANAAGGTCAGAAATGTGAATTNCCAGATGCCTATGTTCTGNTGAGTGAAAAGAAAT TTCTAGTATCCAGTCCATTGTACCTGCTNCTGAAATTGCAATGCTNACCGNTAGCCTTGG NTCATNATCGCTGAAGAATGTGATGGAGAAAGCTCTAGTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_002156 unedited GCACTTCCAGGGCCGGAATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGT TCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTA ACTTTAAACAAATTTTTATTACACAAAGGTTGTCACATAATTGGATACTTCTCTACTTTG TACACAATTATTCTCACTCTCCACAGAAAGGCTGCTTAACTTCTCATCTGGTGGTGGCAA GCACTAAAATCCTGATTTTAACAGAATAGTAGTAAAAATGCCTCAGTGATTTAAGTTGAA AGCAGTACACTGGTACATGGCTCTTGTACCCAGTATCAGGAATGTACAAATGTTTTTTAT TCAAAAATACAAAATAAATTATCTGTAGGCATGGACAATGACAGCAGTAAACCATTATAT ATTTTGTCAACTGAAACCAGTAACTGATGGTTATAGTGATTTTCAGCCAGCCTTTTTCTT CATTTTCTCCAACTGACTTCTCTGAAGTTATTGGTGAGGAACACTGCCTTGGGCTTCCTG TCACAGTTCATTAATAAAGGTAAAGCACTAGTCTAGGAGTTAGAACATGCCACCTCCCAT ACCACCTCCCATTCCACCCATTGCACCCATTCCAGGGTCCTTCTCTTCTTTAGGAATTTC TGTGACTACAACTTCTGCTGTAGTTAACAGAGAGGCCACACCAGCAGCATCCAATAAAGC AGTTCTCACAACCTTTGNTGGGTCAATGATTCCTTTTTCCACCATATTCACAAAATCTNC AGCCATAGCATCATAACCCACTTCTGAGGAACTTTGCATAANTTTCTCACTATCAAAGAT CCTTTCACACCTGCATTCTTAGCATGGNCATTGCTGGAAATTTGGAGTGTCTC
Restriction Sites	NotI-NotI
ACCN	NM_002156
Insert Size	2240 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_002156.4, NP_002147.2
RefSeq Size	2339 bp
RefSeq ORF	1722 bp
Locus ID	3329
UniProt ID	P10809
Domains	cpn60_TCP1
Protein Families	Druggable Genome, Stem cell - Pluripotency
Protein Pathways	RNA degradation, Type I diabetes mellitus
Gene Summary	This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (2)

The use of this cDNA Clones has been cited in the following citations:
secHsp70 as a tool to approach amyloid-β42 and other extracellular amyloids ,de Mena, L;Chhangani, D;Fernandez-Funez, P;Rincon-Limas, DE;, Fly (Austin) 2017 ,PubMed ID 28165856 [HSPD1]
Oxidant-induced apoptosis is mediated by oxidation of the actin-regulatory protein cofilin ,Fábio Klamt, Stéphanie Zdanov, Rodney L. Levine, Ashley Pariser, Yaqin Zhang, Baolin Zhang, Li-Rong Yu, Timothy D. Veenstra, Emily Shacter, Nature Cell Biology 11, 1241-1246 (6 September 2009) doi:10.1038/ncb1968 Letter [HSPD1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC224428	HSPD1 (Myc-DDK-tagged)-Human heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,264.00 CNY 4,560.00
RC224428L1	Lenti ORF clone of Human heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,664.00
RC224428L2	Lenti ORF clone of Human heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RC224428L3	Lenti ORF clone of Human heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,460.00
RC224428L4	Lenti ORF clone of Human heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,664.00
RG224428	HSPD1 (tGFP-tagged) - Human heat shock 60kDa protein 1 (chaperonin) (HSPD1), nuclear gene encoding mitochondrial protein, transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,864.00

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Proteins

Antibodies