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TRPM8 (NM_024080) Human Untagged Clone
CAT＃: SC112270
TRPM8 (untagged)-Human transient receptor potential cation channel, subfamily M, member 8 (TRPM8)
CNY 11,544.00
| Cited in 2 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LTrpC-6; LTRPC6; trp-p8; TRPP8 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_024080 edited
ATGTCCTTTCGGGCAGCCAGGCTCAGCATGAGGAACAGAAGGAATGACACTCTGGACAGC ACCCGGACCCTGTACTCCAGCGCGTCTCGGAGCACAGACTTGTCTTACAGTGAAAGCGAC TTGGTGAATTTTATTCAAGCAAATTTTAAGAAACGAGAATGTGTCTTCTTTACCAAAGAT TCCAAGGCCACGGAGAATGTGTGCAAGTGTGGCTATGCCCAGAGCCAGCACATGGAAGGC ACCCAGATCAACCAAAGTGAGAAATGGAACTACAAGAAACACACCAAGGAATTTCCTACC GACGCCTTTGGGGATATTCAGTTTGAGACACTGGGGAAGAAAGGGAAGTATATACGTCTG TCCTGCGACACGGACGCGGAAATCCTTTACGAGCTGCTGACCCAGCACTGGCACCTGAAA ACACCCAACCTGGTCATTTCTGTGACCGGGGGCGCCAAGAACTTCGCCCTGAAGCCGCGC ATGCGCAAGATCTTCAGCCGGCTCATCTACATCGCGCAGTCCAAAGGTGCTTGGATTCTC ACGGGAGGCACCCATTATGGCCTGATGAAGTACATCGGGGAGGTGGTGAGAGATAACACC ATCAGCAGGAGTTCAGAGGAGAATATTGTGGCCATTGGCATAGCAGCTTGGGGCATGGTC TCCAACCGGGACACCCTCATCAGGAATTGCGATGCTGAGGGCTATTTTTTAGCCCAGTAC CTTATGGATGACTTCACAAGAGATCCACTCTATATCCTGGACAACAACCACACACATTTG CTGCTCGTGGACAATGGCTGTCATGGACATCCCACTGTCGAAGCAAAGCTCCGGAATCAG CTAGAGAAGTATATCTCTGAGCGCACTATTCAAGATTCCAACTATGGTGGCAAGATCCCC ATTGTGTGTTTTGCCCAAGGAGGTGGAAAAGAGACTTTGAAAGCCATCAATACCTCCATC AAAAATAAAATTCCTTGTGTGGTGGTGGAAGGCTCGGGCCAGATCGCTGATGTGATCGCT AGCCTGGTGGAGGTGGAGGATGCCCTGACATCTTCTGCCGTCAAGGAGAAGCTGGTGCGC TTTTTACCCCGCACGGTGTCCCGGCTGCCTGAGGAGGAGACTGAGAGTTGGATCAAATGG CTCAAAGAAATTCTCGAATGTTCTCACCTATTAACAGTTATTAAAATGGAAGAAGCTGGG GATGAAATTGTGAGCAATGCCATCTCCTACGCTCTATACAAAGCCTTCAGCACCAGTGAG CAAGACAAGGATAACTGGAATGGGCAGCTGAAGCTTCTGCTGGAGTGGAACCAGCTGGAC TTAGCCAATGATGAGATTTTCACCAATGACCGCCGATGGGAGTCTGCTGACCTTCAAGAA GTCATGTTTACGGCTCTCATAAAGGACAGACCCAAGTTTGTCCGCCTCTTTCTGGAGAAT GGCTTGAACCTACGGAAGTTTCTCACCCATGATGTCCTCACTGAACTCTTCTCCAACCAC TTCAGCACGCTTGTGTACCGGAATCTGCAGATCGCCAAGAATTCCTATAATGATGCCCTC CTCACGTTTGTCTGGAAACTGGTTGCGAACTTCCGAAGAGGCTTCCGGAAGGAAGACAGA AATGGCCGGGACGAGATGGACATAGAACTCCACGACGTGTCTCCTATTACTCGGCACCCC CTGCAAGCTCTCTTCATCTGGGCCATTCTTCAGAATAAGAAGGAACTCTCCAAAGTCATT TGGGAGCAGACCAGGGGCTGCACTCTGGCAGCCCTGGGAGCCAGCAAGCTTCTGAAGACT CTGGCCAAAGTGAAGAACGACATCAATGCTGCTGGGGAGTCCGAGGAGCTGGCTAATGAG TACGAGACCCGGGCTGTTGAGCTGTTCACTGAGTGTTACAGCAGCGATGAAGACTTGGCA GAACAGCTGCTGGTCTATTCCTGTGAAGCTTGGGGTGGAAGCAACTGTCTGGAGCTGGCG GTGGAGGCCACAGACCAGCATTTCATCGCCCAGCCTGGGGTCCAGAATTTTCTTTCTAAG CAATGGTATGGAGAGATTTCCCGAGACACCAAGAACTGGAAGATTATCCTGTGTCTGTTT ATTATACCCTTGGTGGGCTGTGGCTTTGTATCATTTAGGAAGAAACCTGTCGACAAGCAC AAGAAGCTGCTTTGGTACTATGTGGCGTTCTTCACCTCCCCCTTCGTGGTCTTCTCCTGG AATGTGGTCTTCTACATCGCCTTCCTCCTGCTGTTTGCCTACGTGCTGCTCATGGATTTC CATTCGGTGCCACACCCCCCCGAGCTGGTCCTGTACTCGCTGGTCTTTGTCCTCTTCTGT GATGAAGTGAGACAGTGGTACGTAAATGGGGTGAATTATTTTACTGACCTGTGGAATGTG ATGGACACGCTGGGGCTTTTTTACTTCATAGCAGGAATTGTATTTCGGCTCCACTCTTCT AATAAAAGCTCTTTGTATTCTGGACGAGTCATTTTCTGTCTGGACTACATTATTTTCACT CTAAGATTGATCCACATTTTTACTGTAAGCAGAAACTTAGGACCCAAGATTATAATGCTG CAGAGGATGCTGATCGATGTGTTCTTCTTCCTGTTCCTCTTTGCGGTGTGGATGGTGGCC TTTGGCGTGGCCAGGCAAGGGATCCTTAGGCAGAATGAGCAGCGCTGGAGGTGGATATTC CGTTCGGTCATCTACGAGCCCTACCTGGCCATGTTCGGCCAGGTGCCCAGTGACGTGGAT GGTACCACGTATGACTTTGCCCACTGCACCTTCACTGGGAATGAGTCCAAGCCACTGTGT GTGGAGCTGGATGAGCACAACCTGCCCCGGTTCCCCGAGTGGATCACCATCCCCCTGGTG TGCATCTACATGTTATCCACCAACATCCTGCTGGTCAACCTGCTGGTCGCCATGTTTGGC TACACGGTGGGCACCGTCCAGGAGAACAATGACCAGGTCTGGAAGTTCCAGAGGTACTTC CTGGTGCAGCAGTACTGCAGCCGCCTCAATATCCCCTTCCCCTTCATCGTCTTCGCTTAC TTCTACATGGTGGTGAAGAAGTGCTTCAAGTGTTGCTGCAAGGAGAAAAACATGGAGTCT TCTGTCTGCTGTTTCAAAAATGAAGACAATGAGACTCTGGCATGGGAGGGTGTCATGAAG GAAAACTACCTTGTCAAGATCAACACAAAAGCCAACGACACCTCAGAGGAAATGAGGCAT CGATTTAGACAACTGGATACAAAGCTTAATGATCTCAAGGGTCTTCTGAAAGAGATTGCT AATAAAATCAAATAA >OriGene 5' read for NM_024080 unedited
TTTACATTAGGTAATACGACTTACTATAGGGCGGCCGCGAAATTCGCACCAGNANAAAAT CCTGCTTGACAAAAACCGTCACTTAGGAAAAGAGTCCTTTCGGGCAGCCAGGCTCAGCAT GAGGAACAGAAGGAATGACACTCTGGACAGCACCCGGACCCTGTACTCCAGCGCGTCTCG GAGCACAGACTTGTCTTACAGTGAAAGCGACTTGGTGAATTTTATTCAAGCAAATCTTAA GAAACGAGAATGTGTCTTCTTTACCAAAGATTCCAAGGCCACGGAGAATGTGTGCAAGTG TGGCTATGCCCAGAGCCAGCATATGGAAGGCACCCAGATCAACCAAAGTGAGAAATGGAA CTACAAGAAACACACCAAGGAATTTCCTACCGACGCCTTTGGGGATATTCAGTTTGAGAC ACTGGGGAAGAAAGGGAAGTATATACGTCTGTCCTGCGACACGGACGCGGAAATCCTTTA CGAGCTGCTGACCCAGCACTGGCACCTGAAAACACCCAACCTGGTCATTTCTGTGACCGG GGGCGCCAAGAACTTCGCCCTGAAGCCGCGCATGCGCAAGATCTTCAGCCGGCTCATCTA CATCGCGCAGTCCAAAGGTGCTTGGCTTCTCACGGGAGGCACCCATTATGGCCCTGATGA AATACATTGGGCGAGGTGGTGAAGAGATAACACCATCAGCAGGAGCTCAGAGGAGAATAT GTGGCCATTGGCATAGCAGCTTTGGGCATGGTCTCCAACCGGGACACCCTCCATCAGAAT TGCGAAGCTGAGGGCTATTTTTTAGCCCAGTACCCTATGGATGACTTCACAAGAGATCCA CTTTATATTCTGGAAAACCACCACACAATTTGCTGCTTGTGAACATGCCTGCCATGGCCA TCCACTGTCGAAACAAACCTCCA >OriGene 3' read for NM_024080 unedited
ACTTTGAACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTAGTAGAGAT GTGGTTTTGCCATGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCTGCCCAC CATCCATAATATTTAAATAAGCATATTGACTTCTTCTTAAACTAAGGGTTAAATATAATA TTTTATTTTAATAGTTCAGGTTCATGTATCTCATATCTTATGCTCAAGTTAATAATTCCT ATTTGCTATACATTTATGAAAAAGCCTTATAAATGGTTTTAATAATAAATAGATATTTTA ATATTTAATAATAAATAGATATTTATTGGAATCTAACCTTGGAAAATTTTCCCAAATACA GCAATAGGATTCATGCACAAAGATGCTCATCCCAATGATCAACTGGAGATAGCCTTCAAT GAGCAAAATTATGGTAATCGCTAGAGGTTGCCTATTATGCAATTATTGATATAATGTTTA AGAAGGCTATGAATATAATAAAAACATATTTGATAAAATACTAAGTGAAAAAGCTTACAT AAAAAGTATATATGTAGTATGATAACATTCTGTAAAATTTTGAAAGAGAATTGAGACATA CATAGAAAAAAAATAGACTTGAAAAACCATCCAGAAATTGTTAGCAGTGATTACCTTAAG GTAATGGGGACAATTTATGTTCTTTCTACATCTCTGTATGGGCTAAATTTTCTATAATAG CCAGCTATTATTTTTAATGGAAAAATAATAAATAAGAGGAGTATCCAATAAGAACAAAGT CAGTAGTGCCTTTCAAGGTTGCATTTTGGGCGACTTTTCACATATTTANGAATGGNGGTG GTTCCTTCAATANGAGCCTGGTGAGTCCAAGAGAGCAGAGACTGCCTTGTCACCTNCTCT GACAGTAAGAAGCTCCTGAGAATGAAACACTTTTCNCTGAGAGAGCGGGTCCTCTGCTGC AN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_024080 |
| Insert Size | 5100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_024080.3. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024080.3, NP_076985.3 |
| RefSeq Size | 5674 bp |
| RefSeq ORF | 3315 bp |
| Locus ID | 79054 |
| UniProt ID | Q7Z2W7 |
| Domains | ion_trans |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| Gene Summary | Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationic selectivity. Plays a role in prostate cancer cell migration (PubMed:25559186). Isoform 2 and isoform 3 negatively regulate menthol- and cold-induced channel activity by stabilizing the closed state of the channel.[UniProtKB/Swiss-Prot Function] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Analgesia mediated by the TRPM8 cold receptor in chronic neuropathic pain
,Proudfoot, CJ;Garry, EM;Cottrell, DF;Rosie, R;Anderson, H;Robertson, DC;Walker, SM;Mitchell, R;,
Current biology
,PubMed ID 16920620
[TRPM8]
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Artificial Sweeteners and Salts Producing a Metallic Taste Sensation Activate TRPV1 Receptors
,Celine E Riera, Horst Vogel, Sidney A Simon, and Johannes le Coutre,
Am J Physiol Regul Integr Comp Physiol. 2007 Aug;293(2):R626-34.
[TRPM8]
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Resources
Other Versions
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| RC220615 | TRPM8 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 8 (TRPM8) |
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| RC220615L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 8 (TRPM8), Myc-DDK-tagged |
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| RC220615L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 8 (TRPM8), mGFP tagged |
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| RC220615L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 8 (TRPM8), Myc-DDK-tagged |
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| RC220615L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily M, member 8 (TRPM8), mGFP tagged |
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| RG220615 | TRPM8 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily M, member 8 (TRPM8) |
CNY 9,288.00 |
