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PHD3 (EGLN3) (NM_022073) Human Untagged Clone
CAT＃: SC112867
EGLN3 (untagged)-Human egl nine homolog 3 (C. elegans) (EGLN3)
CNY 2,400.00
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HIFP4H3; HIFPH3; PHD3 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC112867 sequence for NM_022073 edited (data generated by NextGen Sequencing)
ATGCCCCTGGGACACATCATGAGGCTGGACCTGGAGAAAATTGCCCTGGAGTACATCGTG CCCTGTCTGCACGAGGTGGGCTTCTGCTACCTGGACAACTTCCTGGGCGAGGTGGTGGGC GACTGCGTCCTGGAGCGCGTCAAGCAGCTGCACTGCACCGGGGCCCTGCGGGACGGCCAG CTGGCGGGGCCGCGCGCCGGCGTCTCCAAGCGACACCTGCGGGGCGACCAGATCACGTGG ATCGGGGGCAACGAGGAGGGCTGCGAGGCCATCAGCTTCCTCCTGTCCCTCATCGACAGG CTGGTCCTCTACTGCGGGAGCCGGCTGGGCAAATACTACGTCAAGGAGAGGTCTAAGGCA ATGGTGGCTTGCTATCCGGGAAATGGAACAGGTTATGTTCGCCACGTGGACAACCCCAAC GGTGATGGTCGCTGCATCACCTGCATCTACTATCTGAACAAGAATTGGGATGCCAAGCTA CATGGTGGGATCCTGCGGATATTTCCAGAGGGGAAATCATTCATAGCAGATGTGGAGCCC ATTTTTGACAGACTCCTGTTCTTCTGGTCAGATCGTAGGAACCCACACGAAGTGCAGCCC TCTTACGCAACCAGATATGCTATGACTGTCTGGTACTTTGATGCTGAAGAAAGGGCAGAA GCCAAAAAGAAATTCAGGAATTTAACTAGGAAAACTGAATCTGCCCTCACTGAAGACTGA Clone variation with respect to NM_022073.3 >OriGene 5' read for NM_022073 unedited
NGGTCGAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCTCGCCGG GCCAGTCCGGAACGGGTCGTGGAGCTCCGCACCACTCCCGCTGGTTCCCGAAGGCAGATC CCTTCTCCCGAGAGTTGCGAGAAACTTTCCCTTGTCCCCGACGCTGCAGCGGCTCGGGTA CCGTGGCAGCCGCAGGTTTCTGAACCCCGGGCCACGCTCCCCGCGCCTCGGCTTCGCGCT CGTGTAGATCGTTCCCTCTCTGGTTGCACGCTGGGGATCCCGGACCTCGATTCTGCGGGC GAGATGCCCCTGGGACACATCATGAGGCTGGACCTGGAGAAAATTGCCCTGGAGTACATC GTGCCCTGTCTGCACGAGGTGGGCTTCTGCTACCTGGACAACTTCCTGGGCGAGGTGGTG GGCGACTGCGTCCTGGAGCGCGTCAAGCAGCTGCACTGCACCGGGGCCCTGCGGGACGGC CAGCTGGCGGGGCCGCGCGCCGGCGTCTCCAAGCGACACCTGCGGGGCGACCAGATCACG TGGATCGGGGGCAACGAGGAGGGCTGCGAGGCCATCAGCTTCCTCCTGTCCCTCATCGAC AGGCTGGTCCTCTACTGCGGGAGCCGGCTGGGCAAATACTACGTCAAGGAGAGGTCTAAG GCAATGGTGGCTTGCTATCCGGGAAATGGAACAGGTTATGTTCGCCACGTGGACAACCCC AACGGTGATGGTCGCTGCATCACCTGCATCTACTATCTGAACAAGAATTGGGATGCCAAG CTACATGGTGGNGATCCTGCGGGATATTCCAGAGGGGAAATCATTCATAGCAGATGTGGA GCCCATTTTTGACAGACTCCTGTTCTTCTGGTCAGATCGTAGGAACCCACACGAAGTGCA GCCCCTCTACGCAACCA >OriGene 3' read for NM_022073 unedited
GGTAAGAGGCTGCCTTCGAGGNNCNGAAAAAATCTTTTTTTTTTTGTGGTGCCATCTTTA TTCAGAAGTCATTATTTTAGCACAAGGAGCTGAGGCAGAAAATCCAGGTTGCAGGACTGG GAAAAAAGGAGTGTCTGCCCCATGGGACACTTATAAATTGTTCACTATGACAGCAGCCCT TTCTAGAGGTATTAATGGCATTAGGTGGTGGTATTCTCCAGAAACCCCAACAGCCCTGGA TTAAGAACATCTGGAAATTGTTCAGATGACACAAATTTCTCTGTTTCCTGCTGTTAAGGC TTCCGTGATGCCTCTCTGTGGCATCTACCACCTCCATTTTTTTCTTTTCAAACCTCCTCC TTTCAAATCAGGAAGTATACATAAAGTGCAAGTAAGGGTCATTCCCTCGCTGTGCTCCTA GGCTCTTCTCTTGATAGTATTACCGAATCTATCAGGTAAACCGCTGGCCGAGTAGGATGT CTGCAGGAATTTCTGGAGTTAGCAAATAACTTCATCTGGCAAAGAGAGTATCTGAAGATC AACACAGTCTTGGCAAGAAAACATGAAGTACCACACACAAGACAGGGATGTGAAGGATGC AAGAAGTAGCAGGGAGATTGTTGTCACTGAAGAGGCCATCTTTGGATCTCAAAGAATTTA AGAGAATTCAGGAACCGTTACTAAAATGAACAAGGCCAGCAGATTTCAGAGCACGGTCAG TCTTCAGTGAGGGCAGATTCAGGTTTCCTAGTTAAATTCCTGGAATTCTCTTTGGCTTCT GCCCCTTCTTCAGCATCAAAGTACCAGACAGTCATAGCATATCTGGGTGCGTAAGAAGGC TGCACTTCGTGTGGGTCCTACGATCTGACCAGAGAACAGAGTCTGTCCAAATGGGCTCCC CATCTGCTATGAAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022073 |
| Insert Size | 1650 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022073.2, NP_071356.1 |
| RefSeq Size | 2765 bp |
| RefSeq ORF | 720 bp |
| Locus ID | 112399 |
| UniProt ID | Q9H6Z9 |
| Domains | 2OG-FeII_Oxy, P4Hc |
| Protein Families | Druggable Genome |
| Protein Pathways | Pathways in cancer, Renal cell carcinoma |
| Gene Summary | Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210319 | EGLN3 (Myc-DDK-tagged)-Human egl nine homolog 3 (C. elegans) (EGLN3) |
CNY 2,400.00 |
|
| RC210319L1 | Lenti ORF clone of Human egl nine homolog 3 (C. elegans) (EGLN3), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210319L2 | Lenti ORF clone of Human egl nine homolog 3 (C. elegans) (EGLN3), mGFP tagged |
CNY 5,890.00 |
|
| RC210319L3 | Lenti ORF clone of Human egl nine homolog 3 (C. elegans) (EGLN3), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210319L4 | Lenti ORF clone of Human egl nine homolog 3 (C. elegans) (EGLN3), mGFP tagged |
CNY 4,800.00 |
|
| RG210319 | EGLN3 (tGFP-tagged) - Human egl nine homolog 3 (C. elegans) (EGLN3) |
CNY 4,000.00 |
