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PUS7 (NM_019042) Human Untagged Clone
CAT＃: SC113305
PUS7 (untagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7)
CNY 2,400.00
CNY 2,950.00
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IDDABS |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_019042, the custom clone sequence may differ by one or more nucleotides
ATGGAGATGACAGAAATGACTGGTGTGTCGCTGAAACGTGGGGCACTGGTTGTCGAAGATAATGACAGTG GAGTCCCAGTTGAAGAGACAAAAAAACAGAAGCTGTCGGAATGCAGTCTAACCAAAGGTCAAGATGGGCT ACAGAATGACTTTCTGTCCATCAGTGAAGACGTGCCTCGGCCTCCTGACACTGTCAGTACTGGGAAAGGT GGAAAGAATTCTGAGGCTCAGTTGGAAGATGAGGAAGAAGAGGAGGAAGATGGACTTTCAGAGGAGTGCG AGGAGGAGGAATCAGAGAGTTTTGCAGACATGATGAAGCATGGACTCACTGAGGCTGACGTAGGCATCAC CAAGTTTGTGAGTTCTCATCAAGGGTTCTCGGGAATCTTAAAAGAAAGATACTCCGACTTCGTTGTTCAT GAAATAGGAAAAGATGGACGGATCAGCCATTTGAATGACTTGTCCATTCCAGTGGATGAGGAGGACCCTT CAGAAGACATATTTACAGTTTTGACAGCTGAAGAAAAGCAGCGATTGGAAGAGCTCCAGCTGTTCAAAAA TAAGGAAACCAGTGTTGCCATTGAGGTTATCGAGGACACCAAAGAGAAAAGAACCATCATCCATCAGGCT ATCAAATCTCTGTTTCCAGGATTAGAGACAAAAACAGAGGATAGGGAGGGGAAGAAATACATTGTAGCCT ACCACGCAGCTGGGAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCTAGGGGAAGTTA CTGCCACTTCGTACTATATAAGGAAAACAAAGACACCATGGATGCTATTAATGTACTCTCCAAATACTTA AGAGTCAAGCCAAATATATTCTCCTACATGGGAACCAAAGATAAAAGGGCTATAACAGTTCAAGAAATTG CTGTTCTCAAAATAACTGCACAAAGACTTGCCCACCTGAATAAGTGCTTGATGAACTTTAAGCTAGGGAA TTTCAGCTATCAAAAAAACCCACTGAAATTGGGAGAGCTTCAAGGAAACCACTTCACTGTTGTTCTCAGA AATATAACAGGAACTGATGACCAAGTACAGCAAGCTATGAACTCTCTCAAGGAGATTGGATTTATTAACT ACTATGGAATGCAAAGATTTGGAACCACAGCTGTCCCTACGTATCAGGTTGGAAGAGCTATACTACAAAA TTCCTGGACAGAAGTCATGGATTTAATATTGAAACCCCGCTCTGGAGCTGAAAAGGGCTACTTGGTTAAA TGCAGAGAAGAATGGGCAAAGACCAAAGACCCAACTGCTGCCCTCAGAAAACTACCTGTCAAAAGGTGTG TGGAAGGGCAGCTGCTTCGAGGACTTTCAAAATATGGAATGAAGAATATAGTCTCTGCATTTGGCATAAT ACCCAGAAATAATCGCTTAATGTATATTCATAGCTACCAAAGCTATGTGTGGAATAACATGGTAAGCAAG AGGATAGAAGACTATGGACTAAAACCTGTTCCAGGGGACCTCGTTCTCAAAGGAGCCACAGCCACCTATA TTGAGGAAGATGATGTTAATAATTACTCTATCCATGATGTGGTAATGCCCTTGCCTGGTTTCGATGTTAT CTACCCAAAGCATAAAATTCAAGAAGCCTACAGGGAAATGCTCACAGCTGACAATCTTGATATTGACAAC ATGAGACACAAAATTCGAGATTATTCCTTGTCAGGGGCCTACCGAAAGATCATTATTCGTCCTCAGAATG TTAGCTGGGAAGTCGTTGCATATGATGATCCCAAAATTCCACTTTTCAACACAGATGTGGACAACCTAGA AGGGAAGACACCACCAGTTTTTGCTTCTGAAGGCAAATACAGGGCTCTGAAAATGGATTTTTCTCTACCC CCTTCTACTTACGCCACCATGGCCATTCGAGAAGTGCTAAAAATGGATACCAGTATCAAGAACCAGACGC AGCTGAATACAACCTGGCTTCGCTGA >OriGene 5' read for NM_019042 unedited
NGTAATATCACCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATA TAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGG CCGCGAATTCGGCACGAGGCAAAGACCAAAGACCCAACTGCTGCCCTCAGAAAACTACCT GTCAAAAGGTGTGTGGAAGGGCAGCTGCTTCGAGGACTTTCAAAATATGGAATGAAGAAT ATAGTCTCTGCATTTGGCATAATACCCAGAAATAATCGCTTAATGTATATTCATAGCTAC CAAAGCTATGTGTGGAATAACATGGTAAGCAAGAGGATAGAAGACTATGGACTAAAACCT GTTCTAGGGGACCTCGTTCTCAAAGGAGCCACAGCCACCTATATTGAGGAAGATGATGTT AATAATTACTCTATCCATGATGTGGTAATGCCCTTGCCTGGTTTCGATGTTATCTACCCA AAGCATAAAATTCAAGAAGCCTACAGGGAAATGCTCACAGCTGACAATCTTGATATTGAC AACATGAGACACAAAATTCGAGATTATTCCTTGTCAGGGGCCTACCGAAAGATCATTATT CGTCCTCAGAATGTTAGCTGGGAAGTCGTTGCATATGATGATCCCAAAATTCCACTTTTC AACACAGATGTGGACAACCTAGAAGGGAAGACACCACCAGTTNTTGCTTCTGAAGGCAAA TACAGGGCTCTGAAAATGGATTTTTCTCTACCCCCTTCTACTTACGCCACCATGGCCATT CGGAGAGTGCTAAANATGGATACCAGTATCAAGAACCAGACGCAGCTGAATACAACCTGG CTTCGCTGAGCAGTACCCTGTCCACAGATTAAANACGTACACCAGTGTTTGCTTCTGGCT CCTGTGCATTN >OriGene 3' read for NM_019042 unedited
TNANNGATCGATTTTTTTTTTTTTTTTTTGAGGTTTTAAAAATATTTATTACATTTGTTT CTAGAAATCATAGAAAAAAAAATGATCAATTTTGATATACAACTTTAGAAAGGCATATTT ATCCTTTCACACCAGAGAAGTTTATGCAATGCCAGACATGGAAATACCAAAGCCACTGGT GACAAAGGGTAAACGCTACTGATAGAAGAAAGCCTTGTCCATTTTTATAGTTTTCTTCTC TACCATTATTTATTTTTGAGCAAATACCAGTCCAGTGGAAAGAGTTCTGAACATAAAAGG CACCTAGAGTAAAAATTAGACCTTAACTAATAAAACAGCTGTACACGTTTACATCAAGAA AAAAAAACCCGGCCAGCACAGCTCATGCCTGTAATCCCAGGAGTTAGCCTCGGCAACATG GCCAAACCCCCGTCTCTACAAAAAAATACAAAAATCAGCTAGGCGTGGTGGTGCGCGCCT GTACTCCCAGCTACTTAAGAGGCTGAGGTGGGAGAGTCACTGGAGCCTGGGAGGTCGAAG CTGCANTGCAAGATTGAAAAATGCAAGATTGCACTGCAATCCACCCTGTGCTACAGAGTG AGATCCTGTCTCGCAAAAAAAAATAAAAAATAAATGGGGCCTATTAATGCCACGTTCTGC CAAGGGATTGATAATATAAATGCCAAACCCCCGCCACCAAAAAGGCTATAATGCTTGCCT GGATCTAGTACTAAACCAGGAAAATGGACATACTTTTTTGTTTATAAATTTGGAGAAGTC TTTATTTTTTCCAGCCCCAGGAGTCCCGGGTTTATTTTTGGGGGGGGCAGCCTTCAAATT TTTTTTTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_019042 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019042.2, NP_061915.1 |
| RefSeq Size | 3484 bp |
| RefSeq ORF | 618 bp |
| Locus ID | 54517 |
| UniProt ID | Q96PZ0 |
| Gene Summary | Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203902 | PUS7 (Myc-DDK-tagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) |
CNY 4,920.00 |
|
| RC203902L3 | Lenti ORF clone of Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203902L4 | Lenti ORF clone of Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7), mGFP tagged |
CNY 5,890.00 |
|
| RG203902 | PUS7 (tGFP-tagged) - Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) |
CNY 6,520.00 |
|
| SC318122 | PUS7 (untagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) |
CNY 7,220.00 |
