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PUS7 (NM_019042) Human Untagged Clone
CAT＃: SC318122
PUS7 (untagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7)
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | IDDABS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318122 representing NM_019042.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGATGACAGAAATGACTGGTGTGTCGCTGAAACGTGGGGCACTGGTTGTCGAAGATAATGACAGT GGAGTCCCAGTTGAAGAGACAAAAAAACAGAAGCTGTCGGAATGCAGTCTAACCAAAGGTCAAGATGGG CTACAGAATGACTTTCTGTCCATCAGTGAAGACGTGCCTCGGCCTCCTGACACTGTCAGTACTGGGAAA GGTGGAAAGAATTCTGAGGCTCAGTTGGAAGATGAGGAAGAAGAGGAGGAAGATGGACTTTCAGAGGAG TGCGAGGAGGAGGAATCAGAGAGTTTTGCAGACATGATGAAGCATGGACTCACTGAGGCTGACGTAGGC ATCACCAAGTTTGTGAGTTCTCATCAAGGGTTCTCGGGAATCTTAAAAGAAAGATACTCCGACTTCGTT GTTCATGAAATAGGAAAAGATGGACGGATCAGCCATTTGAATGACTTGTCCATTCCAGTGGATGAGGAG GACCCTTCAGAAGACATATTTACAGTTTTGACAGCTGAAGAAAAGCAGCGATTGGAAGAGCTCCAGCTG TTCAAAAATAAGGAAACCAGTGTTGCCATTGAGGTTATCGAGGACACCAAAGAGAAAAGAACCATCATC CATCAGGCTATCAAATCTCTGTTTCCAGGATTAGAGACAAAAACAGAGGATAGGGAGGGGAAGAAATAC ATTGTAGCCTACCACGCAGCTGGGAAAAAGGCTTTGGCAAATCCAAGAAAACATTCTTGGCCAAAATCT AGGGGAAGTTACTGCCACTTCGTACTATATAAGGAAAACAAAGACACCATGGATGCTATTAATGTACTC TCCAAATACTTAAGAGTCAAGCCAAATATATTCTCCTACATGGGAACCAAAGATAAAAGGGCTATAACA GTTCAAGAAATTGCTGTTCTCAAAATAACTGCACAAAGACTTGCCCACCTGAATAAGTGCTTGATGAAC TTTAAGCTAGGGAATTTCAGCTATCAAAAAAACCCACTGAAATTGGGAGAGCTTCAAGGAAACCACTTC ACTGTTGTTCTCAGAAATATAACAGGAACTGATGACCAAGTACAGCAAGCTATGAACTCTCTCAAGGAG ATTGGATTTATTAACTACTATGGAATGCAAAGATTTGGAACCACAGCTGTCCCTACGTATCAGGTTGGA AGAGCTATACTACAAAATTCCTGGACAGAAGTCATGGATTTAATATTGAAACCCCGCTCTGGAGCTGAA AAGGGCTACTTGGTTAAATGCAGAGAAGAATGGGCAAAGACCAAAGACCCAACTGCTGCCCTCAGAAAA CTACCTGTCAAAAGGTGTGTGGAAGGGCAGCTGCTTCGAGGACTTTCAAAATATGGAATGAAGAATATA GTCTCTGCATTTGGCATAATACCCAGAAATAATCGCTTAATGTATATTCATAGCTACCAAAGCTATGTG TGGAATAACATGGTAAGCAAGAGGATAGAAGACTATGGACTAAAACCTGTTCCAGGGGACCTCGTTCTC AAAGGAGCCACAGCCACCTATATTGAGGAAGATGATGTTAATAATTACTCTATCCATGATGTGGTAATG CCCTTGCCTGGTTTCGATGTTATCTACCCAAAGCATAAAATTCAAGAAGCCTACAGGGAAATGCTCACA GCTGACAATCTTGATATTGACAACATGAGACACAAAATTCGAGATTATTCCTTGTCAGGGGCCTACCGA AAGATCATTATTCGTCCTCAGAATGTTAGCTGGGAAGTCGTTGCATATGATGATCCCAAAATTCCACTT TTCAACACAGATGTGGACAACCTAGAAGGGAAGACACCACCAGTTTTTGCTTCTGAAGGCAAATACAGG GCTCTGAAAATGGATTTTTCTCTACCCCCTTCTACTTACGCCACCATGGCCATTCGAGAAGTGCTAAAA ATGGATACCAGTATCAAGAACCAGACGCAGCTGAATACAACCTGGCTTCGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019042 |
| Insert Size | 1986 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019042.4 |
| RefSeq Size | 3529 bp |
| RefSeq ORF | 1986 bp |
| Locus ID | 54517 |
| UniProt ID | Q96PZ0 |
| MW | 75 kDa |
| Gene Summary | Pseudouridylate synthase that catalyzes pseudouridylation of RNAs (PubMed:28073919, PubMed:29628141). Acts as a regulator of protein synthesis in embryonic stem cells by mediating pseudouridylation of RNA fragments derived from tRNAs (tRFs): pseudouridylated tRFs inhibit translation by targeting the translation initiation complex (PubMed:29628141). Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3' (PubMed:28073919). In addition to mRNAs and tRNAs, binds other types of RNAs, such as snRNAs, Y RNAs and vault RNAs, suggesting that it can catalyze pseudouridylation of many RNA types (PubMed:29628141).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203902 | PUS7 (Myc-DDK-tagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) |
CNY 4,920.00 |
|
| RC203902L3 | Lenti ORF clone of Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203902L4 | Lenti ORF clone of Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7), mGFP tagged |
CNY 5,890.00 |
|
| RG203902 | PUS7 (tGFP-tagged) - Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) |
CNY 6,520.00 |
|
| SC113305 | PUS7 (untagged)-Human pseudouridylate synthase 7 homolog (S. cerevisiae) (PUS7) |
CNY 2,400.00 |
