TMEM100 (NM_018286) Human Untagged Clone

CAT#: SC113596

TMEM100 (untagged)-Human transmembrane protein 100 (TMEM100), transcript variant 2



  "NM_018286" in other vectors (6)

CNY 1,200.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TMEM100 mouse monoclonal antibody, clone OTI10G3 (formerly 10G3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC113596 sequence for NM_018286 edited (data generated by NextGen Sequencing)
ATGACTGAAGAGCCCATCAAGGAGATCCTGGGAGCCCCAAAGGCTCACATGGCAGCGACG
ATGGAGAAGAGCCCCAAGAGTGAAGTTGTGATCACCACAGTCCCTCTGGTCAGTGAGATT
CAGTTGATGGCTGCTACAGGGGGTACCGAGCTCTCCTGCTACCGCTGCATCATCCCCTTT
GCTGTGGTTGTCTTCATCGCCGGCATCGTGGTCACCGCGGTGGCTTACAGCTTCAATTCC
CATGGGTCTATTATCTCCATCTTTGGCCTGGTTGTTCTGTCATCTGGACTTTTTTTACTA
GCCTCCAGTGCCTTGTGCTGGAAAGTGAGACAAAGGAGCAAGAAAGCCAAGAGACGGGAG
AGTCAAACAGCTCTCGTGGCAAATCAGAGAAGCTTGTTTGCTTGA

Clone variation with respect to NM_018286.2
>OriGene 5' read for NM_018286 unedited
GCACGAGGAGGAAAGCCTTGCTGTTAGAGGCTCTTTCCCATAAGTTGGACGAAGAGGCTC
ATGCGTTGCTGTTTCTTGTCTTCCAAGTCAAGTGGTTACTCTGGTAATGGATTGCCTCTC
TCCGAGGTGAGTCTTTCACCCTGGTGAGACTGTCCAGATCTAGTCTGTAAACCCAGCTTA
TAAGCACTGTTGTAAAAATGACTGAAGAGCCCATCAAGGAGATCCTGGGAGCCCCAAAGG
CTCACATGGCAGCGACGATGGAGAAGAGCCCCAAGAGTGAAGTTGTGATCACCACAGTCC
CTCTGGTCAGTGAGATTCAGTTGATGGCTGCTACAGGGGGTACCGAGCTCTCCTGCTACC
GCTGCATCATCCCCTTTGCTGTGGTTGTCTTCATCGCCGGCATCGTGGTCACCGCGGTGG
CTTACAGCTTCAATTCCCATGGGTCTATTATCTCCATCTTTGGCCTGGTTGTTCTGTCAT
CTGGACTTTTTTTACTAGCCTCCAGTGCCTTGTGCTGGAAAGTGAGACAAAGGAGCAAGA
AAGCCAAGAGACGGGAGAGTCAAACAGCTCTCGTGGCAAATCAGAGAAGCTTGTTTGCTT
GAGACTGAATACGACCAAATGGGCCATTGGGCCTGGAAAACGTGCTCTGACTTTGTCACC
CAATTCACCCAGAACCATGGTGGGAGAGAACAGACTTGGCGTTGGAGCAGACTGGA
>OriGene 3' read for NM_018286 unedited
TACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTCCCAAAATGTAA
TATACATTTAATAGCACATTATAAAGTTCCTGACCAAAGACGTTGATTTCCTAATTATAA
TAGCACAGAAATCCTTTAGAATTTAGTAAACGTAATTAAGACTATTCAGAAGTAATGAAA
AACCAATATGATAAAAACAAAAATCCTCCAGTAAAGAAGGAACCTGTCCATTTGAGAGAA
ATACAATTGAGAACTTGCAAATGAGACAAGGGAAGATGGCAATTTGGAACTGCAATAGAA
ATAACTATAGCAGAAACAACCATTTAAGAAGTTTTAGCAGCAATAAGTATTTATTATTCT
GAATGAAATGTACAGTTGACTTTTATATAAAAATCATCAAAAGTGCTATATTGGATTATT
TTACTATTAATTTAACCCCCAACAGCATCTATTAGCTATAACTTTAATGGGTTCTTCTTT
ACTTCTGATACATCATTACAACTGGCTAACACTGTCCAATGTTAAAATATAAAAAATATT
CCTTTTCAAGATAAAGTACCACCAGTGATGAGTTATTTAAAGTATAAAATGAAAACAGAC
GCCCGGGAATCATGGGTTGAAAAAAATATCACAGAATTTCATAGACCTATGCGGAACCCA
AGGTAATTTAGATCCTTGGAAAACAATTTTTAGGATACATCTTAAGAACTGGGGGTATTT
TTTTTGGGGGGAAATTGTTAGTGGGGCCCGGCCTTACTTGGATTTTCCCACTCTTTTTTG
GGCTCTTTCAGGGGGGAACCAAACCGGGTAGAAGGGGGGCCCTCCTTGACCCCACAAGGT
TTCTGGAAAAAAAAGCGGGTTTTCGGTCTAAAATGCCCTCTGGGTAAGTGGGTGTAAAGA
AACTGGCTTTCCTACAAGGAAAG
Restriction Sites NotI-NotI     
ACCN NM_018286
Insert Size 1680 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_018286.1, NP_060756.1
RefSeq Size 1670 bp
RefSeq ORF 405 bp
Locus ID 55273
UniProt ID Q9NV29
Protein Families Transmembrane
Gene Summary Plays a role during embryonic arterial endothelium differentiation and vascular morphogenesis through the ACVRL1 receptor-dependent signaling pathway upon stimulation by bone morphogenetic proteins, such as GDF2/BMP9 and BMP10. Involved in the regulation of nociception, acting as a modulator of the interaction between TRPA1 and TRPV1, two molecular sensors and mediators of pain signals in dorsal root ganglia (DRG) neurons. Mechanistically, it weakens their interaction, thereby releasing the inhibition of TRPA1 by TRPV1 and increasing the single-channel open probability of the TRPA1-TRPV1 complex.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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