DARS2 (NM_018122) Human Untagged Clone
CAT#: SC113685
DARS2 (untagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein
CNY 7,768.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ASPRS; LBSL; MT-ASPRS; mtAspRS |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_018122, the custom clone sequence may differ by one or more nucleotides
ATGTACTTCCCTTCTTGGTTAAGTCAGCTGTACAGGGGTTTATCCAGACCCATCAGAAGGACCACCCAAC CGATCTGGGGTTCTCTCTACAGAAGTCTGTTGCAGAGTTCACAGAGGAGAATTCCAGAATTCAGTAGCTT TGTTGTCCGGACCAACACATGTGGAGAGTTGCGTTCGTCTCACTTAGGCCAAGAAGTCACCTTGTGTGGA TGGATTCAGTACCGAAGGCAAAACACATTCTTGGTCCTAAGAGATTTCGATGGGCTTGTTCAAGTTATCA TTCCCCAGGATGAGTCGGCAGCCTCTGTGAAGAAGATTTTATGTGAAGCCCCTGTGGAATCTGTGGTGCA AGTGTCTGGTACAGTCATTTCCCGTCCTGCAGGACAAGAGAATCCAAAAATGCCAACAGGTGAGATTGAA ATCAAAGTTAAAACAGCTGAGCTTCTGAATGCCTGCAAGAAGCTGCCCTTTGAAATTAAGAACTTCGTGA AGAAAACAGAGGCTCTTCGGTTGCAGTATCGCTACTTAGACTTGCGTAGTTTCCAAATGCAGTATAACCT GCGACTGAGGTCCCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCATGGGTTTGTGGATATA GAAACCCCCACATTGTTTAAGAGGACCCCAGGGGGTGCCAAAGAGTTTTTAGTACCATCCAGGGAACCTG GAAAGTTTTATTCTCTCCCTCAGAGTCCTCAACAGTTTAAGCAACTTCTGATGGTTGGCGGTTTAGACAG ATATTTTCAGGTTGCCCGATGTTATCGAGATGAAGGTTCAAGACCAGACAGACAGCCTGAGTTTACTCAG ATTGACATAGAGATGTCATTTGTAGACCAGACTGGGATCCAGAGTTTAATTGAGGGTTTGCTCCAGTATT CCTGGCCCAATGACAAAGATCCTGTGGTTGTTCCTTTTCCTACTATGACTTTTGCTGAGGTGCTGGCCAC CTATGGAACTGATAAACCTGACACTCGCTTTGGAATGAAGATTATAGATATCAGTGATGTGTTTAGAAAC ACAGAGATTGGATTTCTTCAAGATGCACTTAGTAAGCCCCATGGAACTGTGAAAGCCATATGTATCCCTG AAGGAGCAAAATACTTAAAAAGGAAAGACATTGAATCCATTAGAAACTTTGCAGCTGACCATTTTAATCA GGAAATCTTACCTGTATTCCTTAACGCCAATAGAAACTGGAATTCTCCAGTTGCTAATTTCATAATGGAG TCACAAAGACTGGAATTAATCAGACTAATGGAGACCCAAGAGGAAGATGTGGTCCTACTAACTGCTGGAG AGCACAATAAAGCATGCTCTTTGTTAGGAAAATTACGACTGGAATGTGCTGACCTTCTAGAAACAAGAGG AGTGGTGCTCCGTGACCCCACTCTGTTCTCTTTCCTTTGGGTGGTAGATTTCCCACTCTTCCTGCCCAAG GAGGAAAATCCCAGAGAGCTGGAATCGGCCCACCACCCATTTACTGCTCCCCACCCCAGTGACATACATC TCCTGTACACTGAGCCCAAAAAGGCCCGTAGCCAACACTATGACTTGGTTTTAAATGGCAATGAAATAGG AGGTGGTTCAATTCGAATTCACAATGCAGAGCTGCAGCGTTATATCCTGGCAACCTTACTAAAGGAGGAT GTGAAAATGCTCTCCCATCTGCTCCAGGCTTTAGATTATGGGGCACCCCCTCATGGAGGAATTGCCTTAG GGTTAGACAGACTGATATGCCTTGTCACTGGATCTCCAAGCATCAGAGATGTCATAGCCTTCCCAAAGTC CTTCCGGGGACATGACCTCATGAGCAATACCCCAGATTCTGTCCCTCCTGAGGAACTGAAGCCCTATCAT ATCCGAGTCTCCAAGCCAACAGACTCCAAAGCAGAAAGAGCTCATTGA >OriGene 5' read for NM_018122 unedited
GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTCTCTCTAC AGAAGTCTGTTGCAGAGTTCACAGAGGAGAATTCCAGAATTCAGTAGCTTTGTTGTCCGG ACCAACACATGTGGAGAGTTGCGTTCGTCTCACTTAGGCCAAGAAGTCACCTTGTGTGGA TGGATTCAGTACCGAAGGCAAAACACATTCTTGGTCCTAAGAGATTTCGATGGGCTTGTT CAAGTTATCATTCCCCAGGATGAGTCGGCAGCCTCTGTGAAGAAGATTTTATGTGAAGCC CCTGTGGAATCTGTGGTGCAAGTGTCTGGTACAGTCATTTCCCGTCCTGCAGGACAAGAG AATCCAAAAATGCCAACAGGTGAGATTGAAATCAAAGTTAAAACAGCTGAGCTTCTGAAT GCCTGCAAGAAGCTGCCCTTTGAAATTAAGAACTTCGTGAAGAAAACAGAGGCTCTTCGG TTGCAGTATCGCTACTTAGACTTGCGTAGTTTCCAAATGCAGTATAACCTGCGACTGAGG TCCCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCATGGGTTTGTGGATATA GAAACCCCCACATTGTTTAAGAGGACCCCAGGGGGTGCCAAAGAGTTTTTAGTACCATCC AGGGAACCTGGNAAGTTTTATTCTCTCCCTCAGAGTCCTCAACAGTTTAAGCAACTTCTG ATGGTTGGCGGGTTAGACAGATATTTTCAAGTTGCCCGATGTTATCGAGATGAAGTTTCA AGACCAGACAGACAGCCCTNAGTTTACTCAGATTGACATAGAGATGTCATTTGTAGACCA GACTGGGATCCAGAGTTTATATGAGGGTTTGCTCCAGT >OriGene 3' read for NM_018122 unedited
GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTATAATTATTAGTTTATTAAAAC TTTTATGTGTATGATTAGAACTTAAAATCACAATGAGTTTTGGTGTTCACTTTTTCGCAC ATTTGAGGTTAATCTAAGGTTGATTAGAATTTTCAATTACTTATAATTGAGAGAAAATAG TGTTCAGTGATTAATAATATCTTTGTTGTCTTTTTTTTTTTTGAGATGGAGTCTTGCTCT GTCACCCAGGCTGGAGTGTAATGGCACCATCTTGGCTCACTGCAACCTCCATCTCCCAGG TTCAAGCAAACCTCATGCCTCAGCCTCCCGAGTGGCTGCGATTACAGGCATGCGCCACCA TGCCTGGCTAATTTTTGTGTTTTTAGTAGAGACGGTTTCACTATGTTGGCCAGGCTGGTG TTGAACTCCTGACCTCAAGTGATCCACCCACCTCAATCTCCCAAAGTGCTGGGATTACAG GTGTGAGCCACCACTCCCGGCCAATAATATCTTTAAAACTAAAAAAAAAACAGAAAATTG ATAAGTAAAATGACTTTTACTATTGATATCAGTTNTGTTTAATCTGAAACTATAAAATAG AGATATATCTACCTATTTATGGATGGATAATACCATCATCTCATATTTGGGGAATGCTTT ATAGTTTTTCAAAGTACTTTTATTCACTTTATCTTCCATATATGGGATCCTCTCTGATTC CAGCAATAACAGCCCGGTGAGGTAGCCAGGGCAAGTATGTTTTTTACACTTTAACAGGAA GGGAAGCTAAGCGAGGTTTATGTACTTACTCAGCCTGAAACACTGAAGAAAAATTGTGAC TCTCATTTCAGGGAGGTTTCTGCTTATTAAAAATTTAGCCCCTCCTCCTTATATTGGGGG GGTGGAAGGCCTTTTAACCTAATTTAATGATCCTCTGCGAGGGGAGAAACATGGTCCTTC CTCCTATTTACGGG |
Restriction Sites | NotI-NotI |
ACCN | NM_018122 |
Insert Size | 3370 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_018122.1, NP_060592.1 |
RefSeq Size | 3348 bp |
RefSeq ORF | 3348 bp |
Locus ID | 55157 |
UniProt ID | Q6PI48 |
Domains | GAD, tRNA-synt_2 |
Protein Pathways | Aminoacyl-tRNA biosynthesis |
Gene Summary | The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207360 | DARS2 (Myc-DDK-tagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein |
CNY 4,800.00 |
|
RC207360L1 | Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 7,200.00 |
|
RC207360L2 | Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,460.00 |
|
RC207360L3 | Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 6,460.00 |
|
RC207360L4 | Lenti ORF clone of Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 6,460.00 |
|
RG207360 | DARS2 (tGFP-tagged) - Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein |
CNY 6,400.00 |
|
SC318102 | DARS2 (untagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein |
CNY 7,220.00 |