DNAJB12 (NM_017626) Human Untagged Clone
CAT#: SC114056
DNAJB12 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2
CNY 3,656.00
CNY 6,650.00
Cited in 1 publication. |
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | DJ10 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_017626, the custom clone sequence may differ by one or more nucleotides
ATGTCATCACTCCGCGCCCGGCTGCCCGCGACGCGCCGGCGGGTGGCGCAGCCCTTCGCTCGCCCGGCCT CCCCCTCCCTGGTTCCGCGTTCTGGTTCCGCCATGGAATCCAACAAGGATGAAGCTGAGCGCTGTATCAG CATCGCCCTCAAGGCCATCCAGAGCAACCAGCCCGACCGGGCGCTCCGCTTCCTGGAGAAGGCACAGCGG CTGTATCCGACGCCGCGAGTTCGCGCCCTGATTGAGTCCCTCAACCAGAAACCACAGACTGCCGGTGACC AACCCCCACCCACAGACACAACCCATGCCACCCACAGGAAAGCAGGTGGGACCGATGCCCCCTCGGCCAA CGGTGAAGCTGGAGGAGAGAGCACCAAAGGCTACACTGCAGAACAGGTTGCAGCTGTGAAAAGGGTCAAG CAATGTAAAGATTACTATGAGATCCTGGGGGTGAGCAGAGGGGCCTCGGATGAGGACCTGAAGAAGGCCT ACCGCAGACTGGCCCTCAAATTCCACCCAGACAAGAACCACGCACCTGGTGCCACTGAAGCCTTCAAAGC CATTGGCACAGCATATGCGGTACTCAGCAACCCGGAGAAGAGGAAGCAGTATGACCAGTTCGGCGATGAC AAGAGCCAGGCGGCCCGGCACGGCCATGGGCATGGGGATTTCCACCGTGGCTTTGAGGCCGACATCTCCC CTGAAGACCTCTTCAACATGTTCTTTGGCGGCGGCTTCCCTTCTAGTAACGTCCACGTCTACAGCAACGG CCGCATGCGCTATACCTACCAGCAAAGGCAGGACCGCAGGGACAACCAGGGTGATGGCGGGCTAGGGGTG TTTGTGCAGCTGATGCCTATCCTCATCCTGATTCTCGTGTCAGCTCTCAGCCAGCTCATGGTCTCCAGTC CACCCTACAGTCTGAGTCCAAGACCGTCCGTGGGCCACATCCACAGGCGAGTCACTGACCACCTGGGTGT CGTCTACTATGTGGGAGACACTTTCTCCGAAGAGTACACAGGCTCCAGCCTCAAAACAGTCGAGCGGAAT GTGGAAGATGATTATATCGCCAACCTCCGGAACAACTGCTGGAAGGAGAAGCAGCAGAAGGAAGGCTTGC TGTACCGGGCACGCTACTTTGGCGACACAGATATGTACCACAGAGCACAGAAGATGGGCACCCCCAGCTG CAGCCGACTGTCAGAGGTGCAGGCCTCCCTGCATGGATAG >OriGene 5' read for NM_017626 unedited
GTGTTCANATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAAGGGCTGGT TCCGCCATGGNAATCCAACAAGNATGAAGCTGAGCGCTGTATCAGCATCGCCCTCAAGGC CATCCAGAGCAACCAGCCCGACCGGGCGCTCCGCTTCCTGGAGAAGGCACAGCGGCTGTA TCCGACGCCGCGAGTTCGCGCCCTGATTGAGTCCCTCAACCAGAAACCACAGACTGCCGG TGACCAACCCCCACCCACAGACACAACCCATGCCACCCACAGGAAAGCAGGTGGGACCGA TGCCCCCTCGGCCAACGGTGAAGCTGGAGGAGAGAGCACCAAAGGCTACACTGCAGAACA GGTTGCAGCTGTGAAAAGGGTCAAGCAATGTAAAGATTACTATGAGATCCTGGGGGTGAG CAGAGGGGCCTCGGATGAGGACCTGAAGAAGGCCTACCGCAGACTGGCCCTCAAATTCCA CCCAGACAAGAACCACGCACCTGGTGCCACTGAAGCCTTCAAAGCCATTGGCACAGCATA TGCGGTACTCAGCAACCCGGAGAAGAGGAAGCAGTATGACCAGTTCGGCGATGACAAGAG CCAGGCGGCCCGGCACGGCCATGGGCATGGGGATTTCCACCGTGGCTTTGAGGCCGACAT CTCCCCTGAAGACCTCTTCAACATGTTCTTTGGCGGCGGCTTCCCTTCTAGTAACGTCCA CGTCTACAGCAACGGCCGCATGCGCTATACCTACCAGCAAAGGCAGGACCGCAGGGACAA CCAGGGTGATGGCGGGCTAGGGGTGTTTGTGCAGCTGATGCCTATCCTCATCCTGATTCT CGTGTCAGCTCTCAGCCAGCTCATGGTCTCCAGTNCACCCTACAGTCTGAGTCCAGACCG TCCGTGGCCACANTCACAGCGAGTACTGACACCTG >OriGene 3' read for NM_017626 unedited
TGTACGCGGCCGCTTCTAAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA CAATTTGAGAATTCTAATTCAAAAAAACAGCTTCCAAAGGATCCTTTAGGATCTATGTTC TTATTGCACATGGCTCAACCCGTCTAGGGACATCTACCAAGACCATATGGTCGGGGCGTG GGGGCAAATCACCGGGGCCACAGTGGGTAGGCACTCTGGCCACATCAGTTCTTATAATCT CTCTGGGCTGAAGTGGCTGCTTCATTGCAGGGTTGGACCTTGTTTAAAAACCAAATTTGA CATTTCCTTACCGAACTTCCTTTCCCAGCAAGAATCCTATTTGTTGGGGGACTTTTAAAA AAAGAGCCGAGAGAAACTCTGGGAGGGTGGTTATCACCTCCTGGCTGGCAGCTTGGGGAA GTAAGGCTTTGGAAGACGGCGGGCGCAATGACCCACCCACCGCCACAGGAAAACATGGTC ATTCCATAAGGCCCAGGAGAAACTGGGGGAATAAATAAAACCTCCCTCCTCCCACTGGCG GCAAGTGCTGTTTTAAGCCAAAATCCCTCATTTTCAATGGGAGGGTAAGAAAACTATTCT GGTTCAGGTGTATCCCTTTGTCCAGGTACCCAGAGGAGGCCTTGGCGTCACTTAACAGCG AGTGAATACAGAGGCAGCCACGGGCACTCGCCTTTCTTCAAGAGTCAAGGAGGTTGCCCT GTGGTCCCGGCACCACTGACTGACTTGGGAAGAAAAACTTTTTGAAATCCTTTGTGGCAT GACTTAGAAAAAACCATGGCAATGAAGGAGCTGTGCCCTGTGGGCCCTTTAAGGAAGCCC CCTTGGGGACTTAAGGGGGGGAAAAGGTTAGTAAATCCTTGGCCCTCTGGAGGCCTTCTT TTTTGGGCAGAGGCTGAAGCTCCTGGCCCAAAAAAAACCCACCCCTTTTGAAGGGAAAGA GTCTCCCAACAGTCTTGGGGCCCTTT |
Restriction Sites | NotI-NotI |
ACCN | NM_017626 |
Insert Size | 3370 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_017626.3, NP_060096.2 |
RefSeq Size | 3215 bp |
RefSeq ORF | 1128 bp |
Locus ID | 54788 |
UniProt ID | Q9NXW2 |
Domains | DnaJ |
Protein Families | Transmembrane |
Gene Summary | DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus; a glycine/phenylalanine (G/F)-rich region; and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1, 2 and 4 encode the same protein. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation. |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Expression of DNAJB12 or DNAJB14 Causes Coordinate Invasion of the Nucleus by Membranes Associated with a Novel Nuclear Pore Structure
,Goodwin, EC;Motamedi, N;Lipovsky, A;Fernández-Busnadiego, R;Dimaio, D;,
PLoS ONE e94322 9 4. April 2014
,PubMed ID 24732912
[DNAJB12]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC220679 | DNAJB12 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2 |
CNY 3,656.00 |
|
RC220679L1 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC220679L2 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC220679L3 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC220679L4 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC229198 | DNAJB12 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2 |
CNY 3,656.00 |
|
RC229198L3 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC229198L4 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG220679 | DNAJB12 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2 |
CNY 5,256.00 |
|
RG229198 | DNAJB12 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily B, member 12 (DNAJB12), transcript variant 2 |
CNY 5,256.00 |
|
SC327833 | DNAJB12 (untagged)-Human DnaJ (Hsp40) homolog subfamily B member 12 (DNAJB12) transcript variant 2 |
CNY 6,650.00 |
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