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PLEK2 (NM_016445) Human Untagged Clone
CAT＃: SC114268
PLEK2 (untagged)-Human pleckstrin 2 (PLEK2)
CNY 7,220.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_016445, the custom clone sequence may differ by one or more nucleotides
ATGGAGGACGGCGTGCTCAAGGAGGGCTTCCTGGTCAAGAGGGGCCACATTGTCCACAACTGGAAGGCGC GATGGTTCATCCTTCGGCAGAACACGCTGGTGTACTACAAGCTTGAGGGGGGTCGGAGAGTGACCCCTCC CAAGGGCCGGATCCTCCTGGATGGCTGCACCATCACCTGCCCCTGCCTGGAGTATGAAAACCGACCGCTC CTCATTAAGCTGAAGACTCAAACATCCACGGAGTACTTCCTGGAGGCCTGTTCTCGAGAGGAGCGGGATG CCTGGGCCTTTGAGATCACCGGGGCTATTCATGCAGGGCAGCCGGGGAAGGTCCAGCAGCTGCACAGCCT GAGAAACTCCTTCAAGCTGCCCCCGCACATCAGCCTGCATCGCATTGTGGACAAGATGCACGATAGCAAC ACCGGAATCCGTTCAAGCCCCAACATGGAGCAGGGAAGCACCTATAAAAAGACCTTCCTCGGCTCCTCCC TGGTGGACTGGCTCATCTCCAACAGCTTCACGGCCAGCCGTCTGGAGGCGGTGACCCTGGCCTCCATGCT CATGGAGGAGAACTTCCTCAGGCCTGTGGGTGTCCGAAGCATGGGAGCCATTCGCTCTGGGGATCTGGCC GAGCAGTTCCTGGATGACTCCACAGCCCTGTACACTTTTGCTGAGAGCTACAAAAAGAAGATAAGCCCCA AGGAAGAAATTAGCCTGAGCACTGTGGAGTTAAGTGGCACGGTGGTGAAACAAGGCTACCTGGCCAAGCA GGGACACAAGAGGAAAAACTGGAAGGTGCGTCGCTTTGTTCTAAGGAAGGATCCAGCTTTCCTGCATTAC TATGACCCTTCCAAAGAAGAGAACAGGCCAGTGGGTGGGTTTTCTCTTCGTGGTTCACTCGTGTCTGCTC TGGAAGATAATGGCGTTCCCACTGGGGTTAAAGGGAATGTCCAGGGAAACCTCTTCAAAGTGATTACTAA GGATGACACACACTATTACATTCAGGCCAGCAGCAAGGCTGAGCGAGCCGAGTGGATTGAAGCTATCAAA AAGCTAACATGA >OriGene 5' read for NM_016445 unedited
TTCAGATATTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACCAGGCCGCGTC GGAGCTGCTTCCTGGGCGCCGTGGGCGCGGACTGCGCGGGCTGCGCGGGTGCCGAGGAGC GCGAGGCGCGGGGAAGGCGCACCTGGGGTGGCCCTGGCGTGCGGGCGGCGACATGGAGGA CGGCGTGCTCAAGGAGGGCTTCCTGGTCAAGAGGGGCCACATTGTCCACAACTGGAAGGC GCGATGGTTCATCCTTCGGCAGAACACGCTGGTGTACTACAAGCTTGAGGGGGGTCGGAG AGTGACCCCTCCCAAGGGCCGGATCCTCCTGGATGGCTGCACCATCACCTGCCCCTGCCT GGAGTATGAAAACCGACCGCTCCTCATTAAGCTGAAGACTCAAACATCCACGGAGTACTT CCTGGAGGCCTGTTCTCGAGAGGAGCGGGATGCCTGGGCCTTTGAGATCACCGGGGCTAT TCATGCAGGGCAGCCGGGGAAGGTCCAGCAGCTGCACAGCCTGAGAAACTCCTTCAAGCT GCCCCCGCACATCAGCCTGCATCGCATTGTGGACAAGATGCACGATAGCAACACCGGAAT CCGTTCAAGCCCCAACATGGAGCAGGGAAGCACCTATAAAAAGACCTTCCTCGGCTCCTC CCTGGTGGACTGGCTCATCTCCAACAGCTTCACGGCCAGCCGTCTGGAGGCGGTGACCCT GGCCTCCATGCTCATGGAGGAGAACTTCCTCANGCCTGTGGNGTGTCCNGAAGCATGGGG AGCCATTCGCTCTGGGGGATCTGGGCCGAGCAGTTCCTGGATGACTCCACAGCCCTGTAC ACTTTTTTGCTGAGAGCTACAANAAGAAGATAAGCCCCAGGAAGAAAATAGCCTGAACTG TGTGNAGTAAGTGGCACGGTGGTGAAACAAGGCTACTGNCCAGCANGACACAGAGAAACT TGGAAGGTGCGTCCCTTTGTCTAAGGAAGATCCAGCTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016445 |
| Insert Size | 1650 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016445.1, NP_057529.1 |
| RefSeq Size | 1479 bp |
| RefSeq ORF | 1062 bp |
| Locus ID | 26499 |
| UniProt ID | Q9NYT0 |
| Domains | DEP, PH |
| Gene Summary | The protein encoded by this gene associates with membrane-bound phosphatidylinositols generated by phosphatidylinositol 3-kinase. The encoded protein then interacts with the actin cytoskeleton to induce cell spreading. In conjunction with complement component 1, q subcomponent, B chain (C1QB), this gene shows an increase in expression in melanoma cells and may serve as an accurate biomarker for the disease. [provided by RefSeq, Dec 2015] |
Documents
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| SDS |
Resources
Other Versions
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|---|---|---|---|
| RC201459 | PLEK2 (Myc-DDK-tagged)-Human pleckstrin 2 (PLEK2) |
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| RC201459L3 | Lenti ORF clone of Human pleckstrin 2 (PLEK2), Myc-DDK-tagged |
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| RC201459L4 | Lenti ORF clone of Human pleckstrin 2 (PLEK2), mGFP tagged |
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| RG201459 | PLEK2 (tGFP-tagged) - Human pleckstrin 2 (PLEK2) |
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| SC322196 | PLEK2 (untagged)-Human pleckstrin 2 (PLEK2) |
CNY 5,488.00 |
