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ARSB (NM_000046) Human Untagged Clone
CAT＃: SC114442
ARSB (untagged)-Human arylsulfatase B (ARSB), transcript variant 1
CNY 3,976.00
CNY 8,550.00
| Cited in 5 publications. |
Product images
CNY 1,999.00
CNY 3,600.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ASB; G4S; MPS6 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114442 sequence for NM_000046 edited (data generated by NextGen Sequencing)
ATGGGTCCGCGCGGCGCGGCGAGCTTGCCCCGAGGCCCCGGACCTCGGCGGCTGCTCCTC CCCGTCGTCCTCCCGCTGCTGCTGCTGCTGTTGTTGGCGCCGCCGGGCTCGGGCGCCGGG GCCAGCCGGCCGCCCCACCTGGTCTTCTTGCTGGCAGACGACCTAGGCTGGAACGACGTC GGCTTCCACGGCTCCCGCATCCGCACGCCGCACCTGGACGCGCTGGCGGCCGGCGGGGTG CTCCTGGACAACTACTACACGCAGCCGCTGTGCACGCCGTCGCGGAGCCAGCTGCTCACT GGCCGCTACCAGATCCGTACAGGTTTACAGCACCAAATAATCTGGCCCTGTCAGCCCAGC TGTGTTCCTCTGGATGAAAAACTCCTGCCCCAGCTCCTAAAAGAAGCAGGTTATACTACC CATATGGTCGGAAAATGGCACCTGGGAATGTACCGGAAAGAATGCCTTCCAACCCGCCGA GGATTTGATACCTACTTTGGATATCTCCTGGGTAGTGAAGATTATTATTCCCATGAACGC TGTACATTAATTGACGCTCTGAATGTCACACGATGTGCTCTTGATTTTCGAGATGGCGAA GAAGTTGCAACAGGATATAAAAATATGTATTCAACAAACATATTCACCAAAAGGGCTATA GCCCTCATAACTAACCATCCACCAGAGAAGCCTCTGTTTCTCTACCTTGCTCTCCAGTCT GTGCATGAGCCCCTTCAGGTCCCTGAGGAATACTTGAAGCCATATGACTTTATCCAAGAC AAGAACAGGCATCACTATGCAGGAATGGTGTCCCTTATGGATGAAGCAGTAGGAAATGTC ACTGCAGCTTTAAAAAGCAGTGGGCTCTGGAACAACACGGTGTTCATCTTTTCTACAGAT AACGGAGGGCAGACTTTGGCAGGGGGTAATAACTGGCCCCTTCGAGGAAGAAAATGGAGC CTGTGGGAAGGAGGCGTCCGAGGGGTGGGCTTTGTGGCAAGCCCCTTGCTGAAGCAGAAG GGCGTGAAGAACCGGGAGCTCATCCACATCTCTGACTGGCTGCCAACACTCATGAAGCTG GCCAGGGGACACACCAATGGCACAAAGCCTCTGGATGGCTTCGACGTGTGGAAAACCATC AGTGAAGGAAGCCCATCCCCCAGAATTGAGCTGCTGCATAATATTGACCCGAACTTCGTG GACTCTTCACCGTGTCCCAGGAACAGCATGGCTCCAGCAAAGGATGACTCTTCTCTTCCA GAATATTCAGCCTTTAACACATCTGTCCATGCTGCAATTAGACATGGAAATTGGAAACTC CTCACGGGCTACCCAGGCTGTGGTTACTGGTTCCCTCCACCGTCTCAATACAATGTTTCT GAGATACCCTCATCAGACCCACCAACCAAGACCCTCTGGCTCTTTGATATTGATCGGGAC CCTGAAGAAAGACATGACCTGTCCAGAGAATATCCTCACATCGTCACAAAGCTCCTGTCC CGCCTACAGTTCTACCATAAACACTCAGTCCCCGTGTACTTCCCTGCACAGGACCCCCGC TGTGATCCCAAGGCCACTGGGGTGTGGGGCCCTTGGATGTAG Clone variation with respect to NM_000046.3 1072 g=>a >OriGene 5' read for NM_000046 unedited
GTCAGCTTTTGTAATACGACTTACNTATAGGGCGGCCGCGATTCGGCACGAGGGACAAGG ATGGGTCCGCGCGGCGCGGCGAGCTTGCCCCGAGGCCCCGGACCTCGGCGGCTGCTCCTC CCCGTCGTCCTCCCGCTGCTGCTGCTGCTGTTGTTGGCGCCGCCGGGCTCGGGCGCCGGG GCCAGCCGGCCGCCCCACCTGGTCTTCTTGCTGGCAGACGACCTAGGCTGGAACGACGTC GGCTTCCACGGCTCCCGCATCCGCACGCCGCACCTGGACGCGCTGGCGGCCGGCGGGGTG CTCCTGGACAACTACTACACGCAGCCGCTGTGCACGCCGTCGCGGAGCCAGCTGCTCACT GGCCGCTACCAGATCCGTACAGGTTTACAGCACCAAATAATCTGGCCCTGTCAGCCCAGC TGTGTTCCTCTGGATGAAAAACTCCTGCCCCAGCTCCTAAAAGAAGCAGGTTATACTACC CATATGGTCGGAAAATGGCACCTGGGAATGTACCGGAAAGAATGCCTTCCAACCCGCCGA GGATTTGATACCTACTTTGGATATCTCCTGNGTAGTGAAGATTATTATTCCCATGAACGC TGTACATTAATTGACGCTCTGAATGTCACACGATGTGCTCTTGATTTTCGAGATGGCGAA GAAGTTGCAACAGGATATAAAAATATGTATTCAACANACATATTCACCAAAAGGGCTATA GCCCTCATAACTAACCATCCACCAGAGAAGCCTCTGTTTCTCTACCTTGCTCTCCAGTCT GTGCATGAGCCNCCTTCAGTCCCTNGAGAATACTTGAAGCCATATGACTTTATCCAAGAC AGAACAGGCATCACTATGCAGGAATGGTGTCCCTTTATGATGAAGCAGTAGGAAATGTCA CTGCAGCTTTTAAAAGCAGTGGGCTCTGGAACAACACGTGTTCATCTTTCTACAGATACN GAGGGCAGACTT >OriGene 3' read for NM_000046 unedited
NGGGTACTCTATGNCCGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTGAGTGTTGCAGATTTTATCAGCTTCTTAAATGCATTAGGGGTTGAAATTA GACACCTCGGTGTGGTTTAAGAGCAAGAGAAGGGCCAAGTGAACCCAGGTTGGGATAACA AATGAGACAAGAGTCGTGAGAAAAGGCCTGAGGTCCAACTTCCAATTGAAAGGTTTTCTA GCCTCCCTGAAATCCTACATCCAAGGGCCCCACACCCCAGTGGCCTTGGGATCACAGCGG GGGTCCTGTGCAGGGAAGTACACGGGGACTGAGTGTTTATGGTAGAACTGTAGGCGGGAC AGGAGCTTTGTGACGATGTGAGGATATTCTCTGGACAGGTCATGTCTTTCTTCAGGGTCC CGATCAATATCAAAGAGCCAGAGGGTCTTGGTTGGTGGGTCTGATGAGGGTATCTCAGAA ACATTGTATTGAGACGGTGGAGGGAACCAGTAACCACAGCCTGGGTAGCCCGTGAGGAGT TTCCAATTTCCATGTCTAATTGCAGCATGGACAGATGTGTTAAAGGCTGAATATTCTGGA AGAGAAGAGTCATCCTTTGCTGGAGCCATGCTGTTCCTGGGACACGGTGAAGAGTCCACG AAGTTCGGGTCAATATTATGCAGCAGCTCAATTCTGGGGGATGGGCTTCCTTCACTGATG GTTTTCCACACGTCGAAGCCATCCAGAGGCTTTGTGCCATTGGTGTGTCCCCTGCCCAGC TTCATGAGTGTTGGCAGCCAGTCAGAGATGTGGATGAAGCTCCGGTTCTTTACGCCCTTT TGCTTTAGCAAGGGGCTTGCCACAAAGCCCACCCTTGGGAAGCCTTCCTTCCACAGCTTC A |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000046 |
| Insert Size | 2000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000046.2, NP_000037.2 |
| RefSeq Size | 6089 bp |
| RefSeq ORF | 1602 bp |
| Locus ID | 411 |
| UniProt ID | P15848 |
| Domains | Sulfatase |
| Protein Families | Druggable Genome |
| Protein Pathways | Glycosaminoglycan degradation, Lysosome, Metabolic pathways |
| Gene Summary | Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targeted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2016] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because transcript sequence consistent with the reference genome assembly was not available for all regions of the RefSeq transcript. The extent of this transcript is supported by transcript alignments. |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Dihydrotestosterone inhibits arylsulfatase B and Dickkopf Wnt signaling pathway inhibitor (DKK)-3 leading to enhanced Wnt signaling in prostate epithelium in response to stromal Wnt3A
,Bhattacharyya, S;Feferman, L;Tobacman, JK;,
Prostate
,PubMed ID 30801800
[ARSB]
|
|
Decline in Arylsulfatase B Expression Increases EGFR Expression by Inhibiting the Protein Tyrosine Phosphatase SHP2 and Activating JNK in Prostate Cells
,Bhattacharyya, S;Feferman, L;Han, X;Ouyang, Y;Zhang, F;Linhardt, RJ;Tobacman, JK;,
J. Biol. Chem.
,PubMed ID 29794138
[ARSB]
|
|
Inhibition of Phosphatase Activity Follows Decline in Sulfatase Activity and Leads to Transcriptional Effects through Sustained Phosphorylation of Transcription Factor MITF
,Bhattacharyya, S;Feferman, L;Tobacman, JK;,
PLoS ONE
,PubMed ID 27078017
[ARSB]
|
|
Distinct effects of N-acetylgalactosamine-4-sulfatase and galactose-6-sulfatase expression on chondroitin sulfates
,Sumit Bhattacharyya, Kumar Kotlo, Sagar Shukla, Robert S. Danziger, and Joanne K. Tobacman,
J. Biol. Chem. April 2008; 283(15): p. 9523-9530
[ARSB]
|
|
AGPAT6 Is a Novel Microsomal Glycerol-3-phosphate Acyltransferase
,Yan Qun Chen, Ming-Shang Kuo, Shuyu Li, Hai H. Bui, David A. Peake, Philip E. Sanders, Stefan J. Thibodeaux, Shaoyou Chu, Yue-Wei Qian, Yang Zhao, David S. Bredt, David E. Moller, Robert J. Konrad, Anne P. Beigneux, Stephen G. Young, and Guoqing Cao,
J. Biol. Chem. 2008; 283(15): p. 10048-10057
[ARSB]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214604 | ARSB (Myc-DDK-tagged)-Human arylsulfatase B (ARSB), transcript variant 1 |
CNY 3,968.00 |
|
| RC214604L1 | Lenti ORF clone of Human arylsulfatase B (ARSB), transcript variant 1, Myc-DDK-tagged |
CNY 6,368.00 |
|
| RC214604L2 | Lenti ORF clone of Human arylsulfatase B (ARSB), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RC214604L3 | Lenti ORF clone of Human arylsulfatase B (ARSB), transcript variant 1, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC214604L4 | Lenti ORF clone of Human arylsulfatase B (ARSB), transcript variant 1, mGFP tagged |
CNY 6,460.00 |
|
| RG214604 | ARSB (tGFP-tagged) - Human arylsulfatase B (ARSB), transcript variant 1 |
CNY 5,568.00 |
