NSDHL (NM_015922) Human Untagged Clone
CAT#: SC114562
NSDHL (untagged)-Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1
CNY 3,656.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | H105E3; SDR31E1; XAP104 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC114562 sequence for NM_015922 edited (data generated by NextGen Sequencing)
ATGGAACCAGCAGTTAGCGAGCCAATGAGAGACCAAGTCGCACGGACTCATTTGACAGAG GACACTCCCAAAGTGAATGCTGACATAGAAAAGGTTAACCAGAATCAGGCCAAGAGATGC ACAGTGATCGGGGGCTCTGGATTCCTGGGGCAGCACATGGTGGAGCAGTTGCTGGCAAGA GGATATGCTGTCAATGTATTTGATATCCAGCAAGGGTTTGATAATCCCCAGGTGCGGTTC TTTCTGGGTGACCTCTGCAGCCGACAGGATCTGTACCCAGCTCTGAAAGGTGTAAACACA GTTTTCCACTGTGCGTCACCCCCACCATCCAGTAACAACAAGGAGCTCTTTTATAGAGTG AATTACATTGGCACCAAGAATGTCATTGAAACTTGCAAAGAGGCTGGGGTTCAGAAACTC ATTTTAACCAGCAGTGCCAGTGTCATCTTTGAGGGCGTCGATATCAAGAATGGAACTGAA GACCTTCCCTATGCCATGAAACCCATTGACTACTACACAGAGACTAAGATCTTACAGGAG AGGGCAGTTCTGGGCGCCAACGATCCTGAGAAGAATTTCTTAACCACAGCCATCCGCCCT CATGGCATTTTCGGCCCAAGGGACCCGCAGTTGGTACCCATCCTCATCGAGGCAGCCAGG AACGGCAAGATGAAGTTCGTGATTGGAAATGGGAAGAACTTGGTGGACTTCACCTTTGTG GAGAACGTGGTCCATGGACACATCCTGGCGGCAGAGCAGCTCTCCCGAGACTCGACACTG GGTGGGAAGGCATTTCACATCACCAATGATGAGCCCATCCCTTTCTGGACATTCCTGTCT CGCATCCTGACAGGCCTCAATTATGAGGCCCCCAAGTACCACATCCCCTACTGGGTGGCC TACTACCTGGCCCTCCTGCTATCCCTGCTGGTGATGGTGATCAGTCCTGTCATCCAGCTG CAGCCCACCTTCACACCCATGCGGGTCGCACTGGCTGGCACATTCCACTACTACAGCTGC GAGAGAGCCAAAAAGGCCATGGGCTACCAGCCACTAGTGACCATGGATGATGCTATGGAG AGGACCGTGCAGAGCTTTCGCCACCTGCGGAGGGTCAAGTGA Clone variation with respect to NM_015922.2 132 t=>g >OriGene 5' read for NM_015922 unedited
NNNGGTTCGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGGT AAAGGGTGGCGGTCCGGGCCTGGAGTTCAGTGGGTGCAGCCTGCTTGCGAGCTGAGGCCA GACAGGGGGGCGCCTACGGACGGAAAAGAAAAGTTGATTACAAACGGGACCATATTTTGC TTCGAAATGGAACCAGCAGTTAGCGAGCCAATGAGAGACCAAGTCGCACGGACTCATTTG ACAGAGGACACTCCCAAAGTGAATGCTGACATAGAAAAGGTTAACCAGAATCAGGCCAAG AGATGCACAGTGATCGGGGGCTCTGGATTCCTGGGGCAGCACATGGTGGAGCAGTTGCTG GCAAGAGGATATGCTGTCAATGTATTTGATATCCAGCAAGGGTTTGATAATCCCCAGGTG CGGTTCTTTCTGGGTGACCTCTGCAGCCGACAGGATCTGTACCCAGCTCTGAAAGGTGTA AACACAGTTTTCCACTGTGCGTCACCCCCACCATCCAGTAACAACAAGGAGCTCTTTTAT AGAGTGAATTACATTGGCACCAAGAATGTCATTGAAACTTGCAAAGAGGCTGGGGTTCAG AAACTCATTTTAACCAGCAGTGCCAGTGTCATCTTTGAGGGCGTCGATATCAAGAATGGA ACTGAAGACCTTCCCTATGCCATGAAACCCATTGACTACTACACAGAGACTAAGATCTTA CAGGAGAGGGCAGTTCTGGGCGCCAACGATCCTGAGAAGAATTTCTTAACCACAGCCATC CGCCCTCATGGCATTTTCGGCCCAGGGACCCGCAGTTGGTACCCATCCTCATCGAGGCAG CCAGGAACGGGCAGATGAAGTTCGTGATTGGAAATGGNAAGAACTTGNNNTGACTCACCT TTGTGGAGAACGTGG >OriGene 3' read for NM_015922 unedited
NGACTCATGGNTAGAGATCATAGTAACTGCCTGCTTCAANAAATCCAAATCCAGTTCCAT GAAGGAAGAAAGTCTGTTTTTGCCGCCCTCATCGTCACGGAAAGAGTAGGGTGCGCTCTC TGCCTAGCAGAAGGAGTCACAGGCTCAGAGCAAACTCATTCAAAGGATGTTATTTCATCA ATCCACAGGGGAAGGAGTGACTGGCTGAGCAACGTGTCTAGAGAGCCCAGCCTCCAGTGT CCCTCACTTGACCCTCCGCAGGTGGCGAAAGCTCTGCACGGTCCTCTCCATAGCATCATC CATGGTCACTAGTGGCTGGTAGCCCATGGCCTTTTTGGCTCTCTCGCAGCTGTAGTAGTG GAATGTGCCAGCCAGTGCGACCCGCATGGGTGTGAAGGTGGGCTGCAGCTGGATGACAGG ACTGATCACCATCACCAGCAGGGATAGCAGGAGGGCCAGGTAGTAGGCCACCCAGTAGGG GATGTGGTACTTGGGGGCCTCATAATTGAGGCCTGTCAGGATGCGAGACAGGAATGTCCA GAAAGGGATGGGCTCATCATTGGTGATGTGAAATGCCTTCCCACCCAGTGTCGAGTCTCG GGAGAGCTGCTCTGCCGCCAGGATGTGTCCATGGACCACGTTCTCCACAAAGGTGAAGTC CACCAAGTTCTTCCCATTTCCAATCACGAACCTCATCTTGCCGTTTCTGGCTGCCTCGAT GAGGATGGGTACCAACTGCGGTTCCCTCGGGCCGAAAATGCCATGAGGGCGGATGGCTGT GGTTAAGAAATTCTCTCAGGATCGTTGGCGCCCAAAACTGCCCTTTCCTGTAAGACCTTA GTCTCTGTGTAGAACTCAATGGGTTCATGGCCTATGGAAGTCCTTAATTCCATTCTTGAT ATCAACCCCTCAAAC |
Restriction Sites | NotI-NotI |
ACCN | NM_015922 |
Insert Size | 1630 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_015922.1, NP_057006.1 |
RefSeq Size | 1563 bp |
RefSeq ORF | 1122 bp |
Locus ID | 50814 |
UniProt ID | Q15738 |
Domains | 3Beta_HSD |
Protein Families | Transmembrane |
Protein Pathways | Metabolic pathways, Steroid biosynthesis |
Gene Summary | The protein encoded by this gene is localized in the endoplasmic reticulum and is involved in cholesterol biosynthesis. Mutations in this gene are associated with CHILD syndrome, which is a X-linked dominant disorder of lipid metabolism with disturbed cholesterol biosynthesis, and typically lethal in males. Alternatively spliced transcript variants with differing 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the more predominant transcript. Transcript variants 1 and 2 encode the same protein. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203225 | NSDHL (Myc-DDK-tagged)-Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1 |
CNY 3,656.00 |
|
RC203225L3 | Lenti-ORF clone of NSDHL (Myc-DDK-tagged)-Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1 |
CNY 5,890.00 |
|
RC203225L4 | Lenti-ORF clone of NSDHL (mGFP-tagged)-Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1 |
CNY 6,056.00 |
|
RG203225 | NSDHL (tGFP-tagged) - Human NAD(P) dependent steroid dehydrogenase-like (NSDHL), transcript variant 1 |
CNY 4,370.00 |