PHF3 (NM_015153) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_015153, the custom clone sequence may differ by one or more nucleotides ATGGATATAGTTGATACATTTAATCATTTAATTCCTACTGAACACTTAGATGATGCCCTATTTCTAGGAT CCAACCTGGAGAATGAAGTCTGTGAGGATTTTAGTGCAAGTCAAAATGTCTTAGAGGACTCGCTGAAGAA CATGCTCAGCGATAAGGATCCTATGCTAGGATCTGCAAGTAACCAGTTCTGTTTGCCTGTTTTGGATAGC AATGATCCCAATTTCCAGATGCCTTGTTCAACAGTTGTTGGTCTTGACGATATTATGGATGAAGGAGTTG TTAAAGAAAGTGGCAATGATACCATTGATGAAGAAGAACTGATTTTACCTAACAGGAACTTAAGGGACAA GGTAGAAGAAAATTCAGTGAGATCTCCAAGAAAATCACCTCGTTTAATGGCACAAGAACAAGTAAGAAGT TTGCGACAGAGCACTATTGCCAAGCGTTCAAATGCAGCACCATTAAGTAACACAAAAAAAGCATCTGGGA AGACTGTATCTACTGCTAAAGCAGGAGTGAAACAACCAGAAAGGAGTCAGGTTAAAGAAGAAGTATGTAT GTCACTGAAACCTGAGTACCATAAGGAGAATAGAAGGTGCAGCCGAAATAGCGGACAAATTGAAGTGGTA CCTGAAGTATCAGTGTCTTCAAGTCATTCTTCAGTGTCATCTTGTCTTGAAATGAAGGATGAAGATGGAT TAGATTCTAAGCATAAGTGTAATAATCCGGGAGAAATAGATGTGCCATCTCATGAATTAAATTGTTCACT TCTTTCAGAGACTTGTGTTACTATTGGAGAAAAGAAAAATGAAGCTTTGATGGAATGTAAAGCCAAGCCT GTTGGTAGTCCATTGTTTAAGTTTTCAGATAAAGAAGAACATGAACAAAATGATTCCATTTCAGGTAAAA CGGGTGAGACTGTTGTTGAAGAAATGATAGCAACAAGAAAAGTTGAACAAGATTCAAAGGAGACAGTAAA ATTATCCCATGAAGATGACCATATTCTTGAGGACGCTGGATCTTCTGATATTTCTAGTGATGCTGCTTGT ACAAATCCAAATAAGACAGAAAACAGCCTTGTAGGTTTGCCTAGTTGTGTAGATGAAGTGACTGAATGTA ATTTGGAATTGAAGGATACCATGGGTATTGCTGATAAAACTGAGAACACCCTTGAAAGAAATAAAATTGA ACCGTTGGGTTATTGTGAAGATGCGGAGTCTAATAGGCAGTTGGAGAGCACTGAGTTTAATAAATCAAAC TTAGAGGTGGTTGATACTAGTACTTTTGGACCGGAAAGTAATATCTTGGAAAATGCTATTTGTGATGTGC CTGACCAAAATTCAAAACAGTTGAATGCTATAGAAAGTACTAAAATAGAGTCCCATGAAACAGCAAACCT TCAGGATGACAGAAACAGCCAGTCAAGTAGCGTTTCTTACTTAGAGTCAAAAAGTGTAAAATCCAAACAT ACAAAACCTGTAATTCATTCTAAGCAAAACATGACCACAGATGCTCCGAAGAAAATTGTTGCAGCAAAGT ATGAAGTAATACATAGCAAAACTAAAGTTAATGTCAAAAGTGTGAAACGAAATACTGATGTACCAGAATC TCAGCAAAATTTTCATAGGCCAGTCAAAGTCAGAAAAAAACAAATTGATAAGGAGCCAAAGATTCAGAGT TGCAATTCTGGGGTTAAATCTGTGAAAAACCAAGCTCATTCTGTACTGAAAAAAACATTACAGGATCAAA CTTTAGTACAAATTTTCAAGCCCTTAACTCATTCTTTGAGTGATAAGTCACACGCTCATCCTGGTTGCTT GAAAGAACCTCATCATCCTGCACAAACTGGACATGTATCACATTCTAGCCAGAAACAGTGTCATAAGCCT CAGCAACAGGCCCCAGCAATGAAAACCAATAGTCACGTGAAGGAAGAGCTTGAACACCCAGGCGTTGAGC ATTTTAAGGAAGAGGATAAACTGAAACTGAAAAAACCTGAGAAGAACCTACAACCCCGCCAAAGAAGAAG CAGCAAAAGTTTTTCTTTAGATGAGCCACCATTGTTCATTCCAGATAACATAGCTACCATAAGAAGAGAA GGCTCTGATCATAGCTCCTCATTTGAAAGCAAATATATGTGGACTCCCAGCAAGCAGTGTGGGTTTTGCA AAAAACCACATGGCAACAGGTTTATGGTTGGCTGTGGGAGATGTGATGACTGGTTTCATGGTGATTGTGT TGGGTTAAGTCTTTCTCAAGCACAGCAGATGGGCGAGGAAGACAAAGAATATGTCTGTGTAAAATGTTGT GCTGAAGAAGACAAAAAGACTGAAATACTAGATCCAGATACTTTGGAAAACCAAGCTACAGTTGAATTCC ATAGTGGAGATAAAACAATGGAGTGTGAAAAGCTTGGATTATCAAAACACACAACAAATGATAGAACCAA ATATATAGATGATACAGTGAAGCACAAGGTCAAAATTTTAAAACGGGAGTCTGGTGAAGGCAGAAATTCA TCAGACTGTAGAGATAATGAAATTAAAAAATGGCAGCTAGCTCCTCTTCGTAAGATGGGACAACCAGTTT TACCTCGGAGATCCTCAGAAGAAAAAAGTGAAAAAATACCGAAAGAGTCTACAACTGTTACTTGCACAGG AGAAAAAGCTTCAAAACCAGGTACTCATGAGAAGCAAGAGATGAAAAAGAAGAAAGTTGAAAAAGGAGTG CTTAATGTACATCCTGCTGCTTCTGCTTCCAAGCCTTCTGCAGATCAGATCAGGCAAAGTGTCAGACATT CTCTCAAAGACATTCTTATGAAGAGACTTACAGACTCAAATTTGAAGGTACCAGAGGAAAAGGCAGCAAA AGTTGCCACAAAAATTGAGAAAGAGCTTTTCTCTTTTTTTCGGGACACAGATGCTAAATATAAGAACAAA TATAGAAGTTTGATGTTTAATTTGAAAGATCCTAAAAACAATATATTATTTAAAAAAGTACTGAAAGGAG AAGTAACTCCTGATCATCTTATCAGAATGAGTCCAGAAGAACTAGCTTCTAAAGAGTTAGCTGCTTGGAG ACGAAGAGAAAACAGACATACCATAGAAATGATTGAGAAAGAGCAGAGAGAAGTGGAACGACGGCCAATC ACCAAAATAACTCATAAAGGTGAAATAGAAATTGAGAGTGATGCCCCAATGAAAGAACAGGAAGCAGCCA TGGAGATTCAGGAACCAGCCGCCAATAAGTCATTGGAGAAGCCAGAAGGATCTGAAAAACAAAAAGAGGA GGTTGACTCTATGTCTAAAGATACCACTAGTCAACACAGACAGCATCTTTTTGATCTCAACTGCAAAATC TGCATAGGTCGAATGGCACCACCTGTAGATGATCTTTCTCCAAAAAAAGTAAAAGTTGTTGTAGGAGTAG CTCGCAAACATTCAGACAATGAAGCAGAAAGTATAGCAGATGCATTATCTTCAACCTCAAATATTTTGGC TTCTGAATTCTTTGAGGAGGAGAAACAGGAGTCTCCAAAGTCAACGTTCTCTCCTGCTCCACGTCCAGAG ATGCCTGGAACTGTTGAAGTTGAGTCTACCTTTCTGGCTCGATTGAACTTCATCTGGAAAGGTTTTATCA ACATGCCTTCTGTGGCAAAATTTGTTACCAAAGCCTATCCAGTATCTGGCTCCCCAGAATACCTGACAGA GGACCTACCAGATAGTATTCAAGTAGGTGGCAGGATATCACCTCAGACAGTTTGGGATTATGTGGAAAAA ATAAAAGCATCAGGAACCAAGGAAATTTGTGTGGTTCGCTTCACACCAGTAACTGAAGAAGATCAAATTT CTTATACTTTGCTCTTTGCATACTTCAGTAGCAGAAAGCGCTATGGAGTAGCTGCTAACAACATGAAGCA GGTTAAAGATATGTACCTTATTCCTTTGGGTGCCACAGATAAAATTCCACACCCTCTTGTGCCTTTTGAT GGACCTGGGCTTGAACTGCATAGACCTAATCTATTGTTGGGCTTAATTATTCGTCAGAAACTGAAGCGAC AGCACAGTGCCTGTGCTAGTACTAGTCATATAGCTGAGACTCCTGAAAGTGCACCACCAATAGCATTGCC ACCTGATAAAAAAAGTAAAATAGAAGTTTCTACAGAAGAAGCACCAGAGGAAGAAAATGACTTTTTTAAT TCTTTTACAACTGTATTACACAAGCAGAGAAATAAACCTCAGCAGAATCTTCAGGAAGACCTTCCAACAG CAGTTGAACCTTTAATGGAAGTCACCAAACAGGAGCCACCAAAACCTTTAAGATTTCTTCCTGGCGTGTT GATTGGCTGGGAGAATCAACCTACTACTCTGGAATTAGCAAATAAACCTCTTCCTGTGGATGATATACTT CAAAGCCTTTTGGGCACCACTGGTCAAGTATATGACCAGGCCCAGTCAGTGATGGAACAAAACACTGTTA AAGAAATTCCATTTTTAAATGAGCAGACCAACTCAAAAATAGAGAAAACAGATAATGTGGAAGTAACTGA TGGTGAAAACAAGGAGATAAAAGTTAAAGTAGATAATATTTCAGAATCTACAGATAAGTCAGCAGAAATA GAAACATCAGTAGTAGGGTCCTCTTCCATTTCTGCAGGGTCTTTGACGAGTCTTAGTCTCAGAGGTAAGC CACCAGATGTTTCTACAGAAGCATTTTTAACAAATTTATCAATTCAGTCAAAACAAGAGGAAACTGTGGA GAGTAAAGAGAAAACATTAAAAAGACAGCTTCAGGAAGATCAAGAGAATAATTTGCAAGATAACCAGACT TCAAATAGTTCTCCATGCAGATCTAATGTAGGAAAAGGAAACATAGATGGTAATGTGAGCTGTAGTGAAA ACCTTGTTGCTAATACAGCGAGGTCTCCACAGTTTATCAACCTGAAAAGGGATCCTAGGCAAGCAGCAGG ACGAAGTCAGCCTGTAACTACTTCAGAAAGCAAAGATGGAGATAGTTGCCGGAATGGAGAAAAACACATG CTGCCTGGCCTGTCACACAACAAGGAGCACTTAACAGAACAAATCAATGTAGAGGAAAAGTTGTGTTCTG CAGAGAAAAACTCGTGTGTTCAGCAGAGTGACAATTTAAAAGTTGCACAAAACTCACCATCAGTAGAAAA CATACAGACTTCTCAAGCAGAACAAGCAAAACCCTTACAGGAGGATATTTTAATGCAAAATATTGAAACT GTGCACCCATTTCGAAGAGGATCAGCAGTAGCGACATCTCATTTTGAAGTTGGAAACACATGTCCATCAG AATTTCCTTCTAAAAGCATCACCTTTACTTCCAGAAGCACCAGCCCCAGAACAAGTACAAACTTTTCACC CATGAGGCCACAGCAGCCCAACCTTCAGCATCTCAAGTCTAGCCCACCTGGATTTCCATTTCCAGGGCCT CCTAATTTTCCCCCACAAAGCATGTTTGGATTTCCACCACATTTGCCACCTCCATTACTTCCCCCTCCAG GCTTTGGCTTTGCTCAAAATCCCATGGTTCCCTGGCCACCTGTTGTTCATCTCCCAGGTCAGCCACAGCG TATGATGGGTCCTCTCTCACAAGCATCAAGGTATATAGGCCCGCAGAATTTTTACCAGGTTAAAGACATT CGGAGGCCAGAAAGGCGCCATAGTGACCCTTGGGGTAGGCAAGACCAACAGCAACTGGATAGGCCATTTA ATAGGGGTAAAGGGGACCGCCAGAGATTTTATAGTGATTCACACCATTTGAAAAGAGAGCGACATGAAAA GGAATGGGAGCAAGAATCTGAAAGGCATAGACGCAGAGACAGAAGCCAAGACAAGGACAGAGACAGAAAA AGCAGGGAGGAAGGGCACAAAGATAAAGAGAGGGCACGGTTATCACATGGTGATCGAGGAACAGATGGAA AAGCAAGCAGAGATAGTAGGAATGTAGACAAGAAGCCAGATAAACCTAAAAGTGAAGACTATGAGAAGGA CAAAGAACGAGAGAAAAGTAAACACAGAGAAGGAGAAAAGGACAGGGATAGGTACCACAAAGATAGGGAC CACACTGACAGAACTAAAAGCAAAAGGTAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_015153 unedited GTATACGACTCACTATAGGGCGGCCGCGATTCGGCACGAGGCTCGTCTGGCGGAGCTGGG GCTGAAAGAACACAGAAGTCTTCATGGATATAGTTGATACATTTAATCATTTAATTCCTA CTGAACACTTAGATGATGCCCTATTTCTAGGATCCAACCTGGAGAATGAAGTCTGTGAGG ATTTTAGTGCAAGTCAAAATGTCTTAGAGGACTCGCTGAAGAACATGCTCAGCGATAAGG ATCCTATGCTAGGATCTGCAAGTAACCAGTTCTGTTTGCCTGTTTTGGATAGCAATGATC CCAATTTCCAGATGCCTTGTTCAACAGTTGTTGGTCTTGACGATATTATGGATGAAGGAG TTGTTAAAGAAAGTGGCAATGATACCATTGATGAAGAAGAACTGATTTTACCTAACAGGA ACTTAAGGGACAAGGTAGAAGAAAATTCAGTGAGATCTCCAAGAAAATCACCTCGTTTAA TGGCACAAGAACAAGTAAGAAGTTTGCGACAGAGCACTATTGCCAAGCGTTCAAATGCAG CACCATTAAGTAACACAAAAAAAGCATCTGGGAAGACTGTATCTACTGCTAAAGCAGGAG TGAAACAACCAGAAAGGAGTCAGGTTAAAGAAGAAGTATGTATGTCACTGAAACCTGAGT ACCATAAGGAGAATAGAAGGTGCAGCCGAAATAGCGGACAAATTGAAGTGGTACCTGAAG TATCAGTGTCTTCAAGTCATTCTTCAGTGTCATCCTGTCTTGAAATGAAAGATGAAGATG GATTAGATTCTAAGCATAAGTGTCATAAATCCGGGGAGAAATAGGATGTGCCATCTCATG AAATTAAATGTTCACTTCTTTCAAAGACTTGTGTTACTATTGGAGAAAAGAAAATGAAGC TTTGATGTAATGTCAAGCCAAGCCTGTTGGTAAT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_015153 unedited NATTTATGNACCGAGGCCCGCAATTTAGGATCGAGTTTTTTTTTTTTTTTTTTTAGGCGG AGAAATTATTTTATCATATTTAGAATCAAAATATCCCTTAAAATATTTACATTTTACAGT AAAAAGGATAGCAAAACACAAGATCATGTACAAGCTTAAGTATTCAAGTTTCTGAAGAGC CAAAAATGAAAATGAAAATAATGGCCTACCACAAAGCTGTAGTATATGGTAATAAATGAC TTCAATGAGTGCAGATTAAAGCAATAAATGTACCCCTTGGGCTTCTGATTCTTGGTCAAA AACTGCTCAACTAAATGATTACCATAATCTGATTATGAAGTTGCTTGGATTTTTGTTGCT CCTCTACAGTTCCTTTAAAAACAGTGCAATTTAAAACAAACTTACAGAGCACACATTACT CCCAAATTAGTCATTATATAAAGGACATTCACTAGAGGAAGCAAATATATTCCATAGTCA ACCATATTTACCAATTAAGGAATAAAACTAAAATTTCTAAGCCTTTATCGCATATCGACA AAACAAATCTTTGAATATACAGCAATGTCAAACTTTTAAAATATTAAAACCCTATAACTA GGGACCGCCGGCTTGAAGAATGGATGATGAGCACTGGCACAGAGCACTTTGTTCAACCAC CACATTTACTCGTCTGCAACGACCAATATTAAAAATTTCCCGCAGCCCCCCCTTCACTGC CACCCTTTTTTCGTTTCCAACAACCACCATTTTACCAGTTATTAAAGGTAATTCTGAAAC AGCCCGACAATCCCCACTTTTGCTTTTACGCCCGCAAGGGGGCCCCCACCTCTTTGTACC CTACCCTGCCCTTTTCCCCTCTCCGGGCAAACCTTTCATTGTTCTTGTTCCTTCCAAAAA CTCCACTATAAGGTTATACTGGCTTCTGGTAAATTCTACTTTCCCTCGTGCTTCCCTCCG TCCCN
Restriction Sites	NotI-NotI
ACCN	NM_015153
Insert Size	6510 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015153.1, NP_055968.1
RefSeq Size	6948 bp
RefSeq ORF	6120 bp
Locus ID	23469
UniProt ID	Q92576
Domains	PHD, TFS2M
Protein Families	Transcription Factors
Gene Summary	This gene encodes a member of a PHD finger-containing gene family. This gene may function as a transcription factor and may be involved in glioblastomas development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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