KIAA0317 (AREL1) (NM_014821) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA0317
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_014821, the custom clone sequence may differ by one or more nucleotides ATGTTTTACGTTATTGGTGGGATCACAGTGTCTGTGGTTGCATTCTTCTTCACAATTAAGTTCCTCTTTG AGCTTGCCGCACGTGTAGTCAGCTTCCTCCAGAATGAGGACCGCGAGCGCCGAGGGGACCGGACTATTTA TGACTACGTGCGGGGAAATTACCTGGATCCCCGGTCTTGCAAAGTCTCCTGGGATTGGAAGGACCCCTAT GAGGTGGGCCACAGCATGGCCTTCCGAGTGCATTTATTCTATAAGAACGGGCAGCCTTTCCCTGCACATC GGCCTGTGGGACTAAGAGTTCACATCTCTCATGTCGAGCTAGCAGTGGAAATTCCAGTGAACCAGGAAGT CCTTCAGGAGCCCAATTCCAACGTAGTAAAAGTGGCCTTCACTGTGCGCAAGGCTGGGCGTTATGAAATC ACAGTGAAGCTTGGTGGATTAAATGTGGCATATAGTCCCTACTACAAAATTTTTCAACCTGGAATGGTGG TTCCTTCTAAGACCAAAATTGTGTGCCACTTTTCTACTCTTGTATTGACCTGTGGGCAGCCGCACACCCT TCAAATAGTACCCCGAGATGAGTATGATAATCCCACCAACAATTCCATGTCCTTGAGAGATGAGCACAAT TACACCTTGTCCATTCATGAGCTCGGCCCTCAAGAAGAAGAGAGTACTGGTGTCTCATTTGAGAAATCAG TAACATCCAACAGGCAGACTTTCCAGGTGTTCTTGCGACTCACCCTGCATTCTCGAGGCTGCTTCCATGC TTGCATTTCATACCAAAATCAGCCAATCAATAATGGTGAATTTGACATTATTGTCCTAAGTGAGGATGAG AAGAATATCGTCGAACGCAATGTGTCCACTTCAGGCGTGAGCATTTACTTTGAGGCTTATCTTTATAATG CTACCAACTGTAGCAGCACTCCATGGCACCTGCCACCCATGCACATGACCTCTTCCCAGCGCCGGCCATC CACTGCTGTTGACGAGGAAGATGAAGACTCGCCCTCTGAGTGCCACACCCCTGAGAAGGTGAAGAAACCG AAGAAGGTGTACTGCTATGTGTCACCAAAGCAATTCTCAGTGAAGGAGTTCTACCTGAAGATCATCCACT GGCGCCTTTACACCTTCCGAGTGTGTCCAGGAACAAAATTTTCATACCTTGGTCCTGACCCTGTCCATAA GCTGCTCACACTGGTGGTGGATGATGGCATTCAACCTCCTGTGGAGCTCAGCTGTAAGGAGAGGAACATT CTAGCAGCCACTTTTATCCGCTCCCTGCATAAGAACATAGGAGGCTCTGAGACCTTTCAGGACAAGGTGA ACTTTTTCCAGCGAGAGCTTCGGCAGGTACATATGAAAAGACCACATTCCAAAGTCACCCTGAAGGTCAG CAGACATGCCTTGTTGGAATCGTCTCTGAAAGCCACTCGGAATTTCTCCATCTCAGATTGGAGCAAGAAC TTTGAGGTTGTTTTCCAGGATGAAGAAGCTCTGGACTGGGGAGGGCCTCGCCGGGAATGGTTTGAGCTAA TCTGCAAAGCACTATTTGATACAACCAATCAGCTCTTCACCCGGTTCAGTGACAACAACCAAGCATTAGT GCATCCCAACCCTAATCGCCCCGCTCATCTGCGCCTGAAAATGTATGAGTTTGCGGGACGGCTCGTGGGC AAGTGTCTCTATGAGTCCTCTCTAGGAGGAGCCTACAAGCAGTTGGTCCGAGCTCGCTTCACCCGCTCTT TCCTGGCCCAAATCATAGGACTGCGTATGCATTACAAGTACTTTGAAACAGATGACCCAGAATTCTACAA ATCTAAAGTTTGTTTTATCCTCAACAATGACATGAGTGAGATGGAGCTGGTCTTTGCAGAAGAGAAATAT AATAAATCAGGTCAATTGGATAAGGTTGTAGAACTCATGACAGGTGGAGCTCAAACTCCAGTCACCAATG CGAATAAAATCTTCTATTTAAATTTGCTGGCCCAATATCGGCTGGCCAGTCAAGTGAAAGAGGAGGTGGA ACATTTCCTAAAAGGCCTGAATGAATTGGTCCCTGAGAACCTTTTGGCTATTTTTGATGAGAATGAGCTT GAGCTGCTGATGTGTGGGACTGGAGACATCAGTGTGTCTGACTTCAAAGCCCATGCAGTAGTTGTTGGTG GCTCATGGCATTTCAGAGAAAAGGTCATGAGGTGGTTTTGGACTGTGGTTTCCAGTCTGACCCAGGAGGA GTTGGCTCGGCTACTTCAGTTCACAACAGGCTCCTCTCAGCTACCACCTGGAGGCTTTGCCGCCCTCTGT CCCTCATTTCAGATTATTGCCGCTCCGACCCATAGCACGCTGCCTACTGCACACACATGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_014821 unedited NNNGGTTCGAATTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGCAGGAA TGATATATTCTTGGGCTGTATTTCTCTACCCTGGAGTTCATCCAGTTCATCCCAGAAACA CTGACCTGATTACTGTGACAAAGTCTGATGGATAGACCCTTTCTGTTGACCTTGTGGCGT TTTTCTTTCATGTGGAAGTTGGAAGACAAGGTGAAAGGGGCCAAAAGTTACCTGCTTTGG TGAATAGGAGTGCTCTACTTGGTTCTCAGAACATGGGCCCTCGTTTAAAGCTGCAGCTGT GATCCTCGGCTGTCTGTTGGCATTGACGGGACCTGATGTTTTACGTTATTGGTGGAATCA CAGTGTCTGTGGTTGCATTCTTCTTCACAATTAAGTTCCTCTTTGAGCTTGCCGCACGTG TAGTCAGCTTCCTCCAGAATGAGGACCGCGAGCGCCGAGGGGACCGGACTATTTATGACT ACGTGCGGGGAAATTACCTGGATCCCCGGTCTTGCAAAGTCTCCTGGGATTGGAAGGACC CCTATGAGGTGGGCCACAGCATGGCCTTCCGAGTGCATTTATTCTATAAGAACGGGCAGC CTTTCCCTGCACATCGGCCTGTGGGACTAAGAGTTCACATCTCTCATGTCGAGCTAGCAG TGGAAATTCCAGTGACCCAGGAAGTCCTTCAGGAGCCCAATTCCAACGTAGTAAAAGTGG CCTTCACTGTGCGCAAGGGCTGGCGTTATGAAATACAGTGAAGCTTGGTGGATTAAATGT GGCATATAGTCCCTTACTACAAATTTTTCAACCTGGAATGGNTGGNTCCTTNCTAGACCC CAAATGNGTGCCACCTTTTCTACNTCTTGTATTGACCTGTGGGCAGCCGCACACCCTTTC AATAGTACCCCGAGATGAGT
Restriction Sites	NotI-NotI
ACCN	NM_014821
Insert Size	6000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_014821.1, NP_055636.1
RefSeq Size	4403 bp
RefSeq ORF	2472 bp
Locus ID	9870
Domains	HECT, Filamin
Protein Families	Druggable Genome, Transmembrane
Gene Summary	E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits apoptosis by ubiquitinating and targeting for degradation a number of proapoptotic proteins including DIABLO/SMAC, HTRA2 and SEPT4/ARTS which are released from the mitochondrion into the cytosol following apoptotic stimulation.[UniProtKB/Swiss-Prot Function]

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