DDX46 (NM_014829) Human Untagged Clone
CAT#: SC114794
DDX46 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46)
CNY 7,184.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | Prp5; PRPF5 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_014829, the custom clone sequence may differ by one or more nucleotides
ATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCTGGAAGTCGGTCTAGAA GTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGACGGTCTAGAAGTAGAGATAGAGATAG GAGGAGAGAGAGGTCTCGTAGCAGGGATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGT TCCAGAAGTAGAGAGAGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAA GGAGTAGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAGAATAG ATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAAGAAAAAAGACAAAGATGACAAG GAGGATGAAAAAGAAAAAGATGCTGGCAACTTTGACCAGAATAAGCTGGAAGAAGAAATGAGAAAGCGAA AAGAAAGAGTAGAAAAATGGCGAGAAGAGCAACGTAAAAAGGCTATGGAAAACATAGGAGAACTGAAAAA GGAAATCGAAGAGATGAAACAAGGGAAAAAGTGGAGTTTAGAGGACGATGATGATGACGAAGATGATCCT GCAGAAGCTGAAAAGGAGGGAAATGAAATGGAGGGTGAGGAGTTAGATCCATTAGATGCTTACATGGAAG AAGTGAAAGAGGAAGTAAAAAAATTTAACATGAGAAGTGTAAAAGGTGGTGGGGGAAATGAAAAGAAGTC TGGGCCAACGGTCACAAAAGTTGTCACTGTTGTGACAACCAAAAAAGCAGTTGTGGATTCTGATAAGAAG AAAGGTGAGCTGATGGAGAATGACCAGGATGCCATGGAGTATTCTTCAGAGGAGGAAGAAGTTGATCTTC AGACAGCCCTTACAGGGTATCAAACAAAACAGCGAAAGCTTCTAGAACCAGTTGATCATGGAAAAATTGA GTATGAGCCATTTAGGAAAAACTTCTATGTTGAAGTTCCAGAACTAGCAAAAATGTCTCAAGAAGAGGTA AATGTGTTTCGATTGGAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCCAAACCAATTAAATCCT GGGTCCAGTGTGGAATTTCCATGAAGATCTTAAATTCCCTCAAGAAGCATGGCTATGAAAAGCCCACGCC CATCCAAACCCAAGCTATTCCTGCTATAATGTCTGGACGAGATTTGATTGGCATTGCCAAAACAGGAAGT GGAAAGACCATTGCTTTTCTGTTGCCCATGTTTAGACACATCATGGATCAGAGGTCATTAGAGGAAGGAG AGGGGCCAATAGCTGTCATCATGACTCCAACTCGAGAACTGGCTTTACAGATTACTAAAGAGTGTAAGAA GTTTTCCAAGACTTTGGGACTTAGAGTGGTCTGTGTTTACGGAGGAACAGGAATCAGTGAGCAGATTGCT GAGCTGAAAAGAGGTGCTGAAATTATTGTTTGCACACCTGGTCGAATGATTGACATGTTAGCCGCTAACA GTGGTCGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGATGAAGCAGACAGAATGTTTGACAT GGGTTTTGAACCCCAGGTCATGCGCATCGTGGATAATGTTCGTCCTGATCGACAGACGGTTATGTTTTCA GCTACTTTCCCCAGAGCTATGGAGGCTTTGGCTCGCAGGATCCTCAGTAAACCTATTGAAGTACAAGTTG GAGGCAGGAGTGTGGTTTGCTCAGATGTGGAGCAACAAGTGATTGTGATTGAAGAAGAAAAGAAATTCTT GAAGTTACTTGAGCTTCTAGGCCATTATCAAGAGTCAGGATCTGTCATTATATTTGTGGATAAGCAGGAA CATGCTGATGGTCTTCTTAAGGATTTAATGAGAGCATCTTATCCTTGCATGTCTCTTCATGGAGGCATTG ATCAATATGACAGAGATAGCATCATAAATGACTTTAAGAATGGGACCTGCAAACTTCTTGTGGCTACCTC TGTTGCTGCCCGAGGTCTAGATGTGAAACATCTGATTCTTGTAGTAAATTATAGCTGCCCCAACCATTAT GAGGATTATGTACACAGAGCAGGGCGGACTGGAAGAGCAGGAAACAAGGGTTATGCTTATACTTTTATCA CAGAGGATCAAGCTCGCTATGCTGGTGACATAATTAAAGCTCTTGAATTGTCAGGGACTGCAGTACCTCC TGATTTAGAGAAACTGTGGAGTGATTTCAAAGATCAGCAGAAAGCTGAGGGGAAAATAATTAAAAAGAGT AGTGGGTTCTCTGGTAAGGGATTCAAGTTTGATGAAACAGAACAAGCTTTGGCTAATGAGAGGAAGAAGT TACAAAAAGCAGCTCTTGGTCTACAAGATTCAGATGATGAGGATGCTGCAGTTGATATTGATGAGCAAAT TGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAGGATATGGCTGCTCCTGGAACATCAAGTGTTCCTGCT CCAACTGCAGGAAATGCTGAGAAATTAGAAATTGCTAAGAGATTGGCTCTTAGAATCAATGCCCAGAAGA ATTTGGGCATCGAGTCTCAGGATGTGATGCAGCAGGCCACCAATGCAATTCTTAGGGGTGGCACCATTCT GGCTCCCACTGTTTCTGCAAAAACCATTGCAGAACAACTTGCTGAAAAGATCAATGCCAAGCTCAATTAT GTGCCGTTAGAGAAACAAGAAGAAGAGAGACAGGATGGTGGACAGAATGAATCTTTTAAGAGATATGAAG AAGAATTAGAGATCAATGACTTCCCACAGACTGCTAGGTGGAAAGTTACCTCTAAGGAAGCTCTGCAGAG AATCAGTGAATACTCTGAAGCCGCAATTACAATCAGAGGAACCTACTTCCCTCCTGGCAAAGAACCCAAG GAAGGCGAGCGGAAGATTTACTTGGCAATTGAAAGTGCCAATGAACTGGCTGTGCAGAAAGCAAAGGCAG AAATCACCAGGCTCATAAAAGAAGAGCTGATCCGGCTGCAAAATTCATACCAACCAACAAATAAAGGAAG ATACAAAGTCTTATAG >OriGene 5' read for NM_014829 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGGATGGCCGCCAC AGCTGTAGGTGCTGCTAGTGTTTAGCGCTGGTCTTTGCCGGGCGTTGAGGGCAGCTCAGC CTCCTTGTTTGTCCGGTTCGCCTGTGCGTGGTACTCAAGGGCACCAGTATTCCCGCGGTC GGCAGCATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGGGGTCGCTCT GGAAGTCGGTCTAGAAGTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGAGATGACAGA CGGTCTAGAAGTAGAGATAGAGATAGGAGGAGAGGAGAGGTCTCGTAGCAGGGATAAAAG AAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGTTCCAGAAGTAGAGAGAGAGACAG AAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAAGGGAGTAGAAGCC GGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAGAATAGA TTCTAGGTCCAAAGAGAAAACTGATGGTGGNGGGAAAGNNTCTAAGAGAAGAANAAAGAA CAAGATGACAAGGAGGATGAAAAAGAANAAGATGCTGGCAACTTTGACCAGAATAAGCTG GGAAGAAGAAATGAGAAAGCGAANAGAAAGAGTAGAAAAATGGCGAAGAGAGCAACGTAN AAAGGCTATGGAAAACATAGGAGAACTGAAAAAGGAAATCGAAGAGATGAACANGGNAAN AAGGGNAGGTTAAAGGACGATGATGATGACAAAAATGATCCTGCAGAAGCTGAAAAGGAA GGAAAGAAATGGAGGGTGAGGAGTTAAATCCCTTAAATGCTTACTGGAAAAAGGAAAGAG GAATTAAAAATTTTACTGAAAAGTGTAAAGGGGGGGGGGGAAATGAAAAAAATTCTGCCA >OriGene 3' read for NM_014829 unedited
ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCCTTTTGTAAGACTTC TATTATAACATTCTTAAATCCAGTTCATATTGTTCCAGAGTCATGTAACATCAAGGTGGG GACACAAAATATGATAGTGAATTTTACCTTTTCTTAGGAAATGATCACAATTTCATTGAA AGTACAGGATAAAGTGAATAGCCCCTTAACGACTTGTGTACTTTATTGTCTTCAATAGAA AGTTCAAATATTTCCATTTTTTAAACCTCTAGAATATTATCCGGACATTTAAAATATTGA TATTTGAGTTTACATTAAAGGACATCATACTCTGAAAACAGGCTGGCTTTAAATATAGTT TGACTGATTGTGGGGGTACTTACGGACAGATTTCTTTACCAAGTACCAGTTTCTTATATT TAAAAAAAATCAGCAAGATAGAATTTAAAAAATCTTCATTTACAATACATTGTAAACTGC AGACAGCAACTGCCACATACATCATAGACCAGCACAGGTAAAAATCTTTTTTCCGGATGT CTATAAGACTTTGTATCTTCCTTTATTTGTTGGTTGGTATGAATTTTGCAGCCGGATCAG CTCTTCTTTTATGAGCCTGGTGATTTCTGCCTTTGCTTTCTGCACAGCCAGTTCATTGGC ACTTTCAATTGCCAAGTAAATCTTCCGCTCGCCTTCCTTGGGTTCTTTGCCAGGAGGGAA GTANGTTCCTCTGATTGTAATTGCGGCTTCAGAGTATTCACTGATTCTCTGCAGAGCTTC TTAGAGGTAACTTTCCCCTAGCAGTCTGTGGGAAGNCATTGATCTCTAAATCTTCTCATA TCTCTAAAAGATNCATTCTGCCACCATCCTGGCTCTCTTCTTNCTGGTTCTCTACGCACA TATTGAGCTGGCATTGATCTTTTCAGCAGTGTCTG |
Restriction Sites | NotI-NotI |
ACCN | NM_014829 |
Insert Size | 4100 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_014829.2, NP_055644.2 |
RefSeq Size | 5695 bp |
RefSeq ORF | 3096 bp |
Locus ID | 9879 |
UniProt ID | Q7L014 |
Domains | DEAD, helicase_C |
Protein Pathways | Spliceosome |
Gene Summary | This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) has an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC202320 | DDX46 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46) |
CNY 7,176.00 |
|
RC202320L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46), Myc-DDK-tagged |
CNY 10,740.00 |
|
RC202320L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46), mGFP tagged |
CNY 10,740.00 |
|
RG202320 | DDX46 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46) |
CNY 9,690.00 |
|
SC324257 | DDX46 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46) |
CNY 7,184.00 |