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DDX46 (NM_014829) Human Untagged Clone
CAT＃: SC324257
DDX46 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46)
CNY 7,184.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | Prp5; PRPF5 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_014829.2
GGCCGCCACAGCTGTAGGTGCTGCTAGTGTTTAGCGCTGGTCTTTGCCGGGCGTTGAGGG
CAGCTCAGCCTCCTTGTTTGTCCGGTTCGCCTGTGCGTGGTACTCAAGGGCACCAGTATT CCCGCGGTCGGCAGCATGGGTCGGGAGTCACGCCACTATCGAAAACGATCGGCATCCCGG GGTCGCTCTGGAAGTCGGTCTAGAAGTCGCTCACCCTCAGACAAAAGAAGTAAACGTGGA GATGACAGACGGTCTAGAAGTAGAGATAGAGATAGGAGGAGAGAGAGGTCTCGTAGCAGG GATAAAAGAAGATCTCGGTCAAGGGACAGGAAGCGTCTGAGACGTTCCAGAAGTAGAGAG AGAGACAGAAGCCGAGAGCGAAGAAGATCTCGAAGTAGAGACAGGAGACGCTCAAGGAGT AGAAGCCGGGGCCGGCGATCCCGATCCTCCAGTCCTGGAAATAAAAGCAAGAAAACTGAG AATAGATCTAGGTCCAAAGAGAAAACTGATGGTGGGGAAAGTTCTAAAGAGAAGAAAAAA GACAAAGATGACAAGGAGGATGAAAAAGAAAAAGATGCTGGCAACTTTGACCAGAATAAG CTGGAAGAAGAAATGAGAAAGCGAAAAGAAAGAGTAGAAAAATGGCGAGAAGAGCAACGT AAAAAGGCTATGGAAAACATAGGAGAACTGAAAAAGGAAATCGAAGAGATGAAACAAGGG AAAAAGTGGAGTTTAGAGGACGATGATGATGACGAAGATGATCCTGCAGAAGCTGAAAAG GAGGGAAATGAAATGGAGGGTGAGGAGTTAGATCCATTAGATGCTTACATGGAAGAAGTG AAAGAGGAAGTAAAAAAATTTAACATGAGAAGTGTAAAAGGTGGTGGGGGAAATGAAAAG AAGTCTGGGCCAACGGTCACAAAAGTTGTCACTGTTGTGACAACCAAAAAAGCAGTTGTG GATTCTGATAAGAAGAAAGGTGAGCTGATGGAGAATGACCAGGATGCCATGGAGTATTCT TCAGAGGAGGAAGAAGTTGATCTTCAGACAGCCCTTACAGGGTATCAAACAAAACAGCGA AAGCTTCTAGAACCAGTTGATCATGGAAAAATTGAGTATGAGCCATTTAGGAAAAACTTC TATGTTGAAGTTCCAGAACTAGCAAAAATGTCTCAAGAAGAGGTAAATGTGTTTCGATTG GAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCCAAACCAATTAAATCCTGGGTC CAGTGTGGAATTTCCATGAAGATCTTAAATTCCCTCAAGAAGCATGGCTATGAAAAGCCC ACGCCCATCCAAACCCAAGCTATTCCTGCTATAATGTCTGGACGAGATTTGATTGGCATT GCCAAAACAGGAAGTGGAAAGACCATTGCTTTTCTGTTGCCCATGTTTAGACACATCATG GATCAGAGGTCATTAGAGGAAGGAGAGGGGCCAATAGCTGTCATCATGACTCCAACTCGA GAACTGGCTTTACAGATTACTAAAGAGTGTAAGAAGTTTTCCAAGACTTTGGGACTTAGA GTGGTCTGTGTTTACGGAGGAACAGGAATCAGTGAGCAGATTGCTGAGCTGAAAAGAGGT GCTGAAATTATTGTTTGCACACCTGGTCGAATGATTGACATGTTAGCCGCTAACAGTGGT CGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGATGAAGCAGACAGAATGTTT GACATGGGTTTTGAACCCCAGGTCATGCGCATCGTGGATAATGTTCGTCCTGATCGACAG ACGGTTATGTTTTCAGCTACTTTCCCCAGAGCTATGGAGGCTTTGGCTCGCAGGATCCTC AGTAAACCTATTGAAGTACAAGTTGGAGGCAGGAGTGTGGTTTGCTCAGATGTGGAGCAA CAAGTGATTGTGATTGAAGAAGAAAAGAAATTCTTGAAGTTACTTGAGCTTCTAGGCCAT TATCAAGAGTCAGGATCTGTCATTATATTTGTGGATAAGCAGGAACATGCTGATGGTCTT CTTAAGGATTTAATGAGAGCATCTTATCCTTGCATGTCTCTTCATGGAGGCATTGATCAA TATGACAGAGATAGCATCATAAATGACTTTAAGAATGGGACCTGCAAACTTCTTGTGGCT ACCTCTGTTGCTGCCCGAGGTCTAGATGTGAAACATCTGATTCTTGTAGTAAATTATAGC TGCCCCAACCATTATGAGGATTATGTACACAGAGCAGGGCGGACTGGAAGAGCAGGAAAC AAGGGTTATGCTTATACTTTTATCACAGAGGATCAAGCTCGCTATGCTGGTGACATAATT AAAGCTCTTGAATTGTCAGGGACTGCAGTACCTCCTGATTTAGAGAAACTGTGGAGTGAT TTCAAAGATCAGCAGAAAGCTGAGGGGAAAATAATTAAAAAGAGTAGTGGGTTCTCTGGT AAGGGATTCAAGTTTGATGAAACAGAACAAGCTTTGGCTAATGAGAGGAAGAAGTTACAA AAAGCAGCTCTTGGTCTACAAGATTCAGATGATGAGGATGCTGCAGTTGATATTGATGAG CAAATTGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAGGATATGGCTGCTCCTGGAACA TCAAGTGTTCCTGCTCCAACTGCAGGAAATGCTGAGAAATTAGAAATTGCTAAGAGATTG GCTCTTAGAATCAATGCCCAGAAGAATTTGGGCATCGAGTCTCAGGATGTGATGCAGCAG GCCACCAATGCAATTCTTAGGGGTGGCACCATTCTGGCTCCCACTGTTTCTGCAAAAACC ATTGCAGAACAACTTGCTGAAAAGATCAATGCCAAGCTCAATTATGTGCCGTTAGAGAAA CAAGAAGAAGAGAGACAGGATGGTGGACAGAATGAATCTTTTAAGAGATATGAAGAAGAA TTAGAGATCAATGACTTCCCACAGACTGCTAGGTGGAAAGTTACCTCTAAGGAAGCTCTG CAGAGAATCAGTGAATACTCTGAAGCCGCAATTACAATCAGAGGAACCTACTTCCCTCCT GGCAAAGAACCCAAGGAAGGCGAGCGGAAGATTTACTTGGCAATTGAAAGTGCCAATGAA CTGGCTGTGCAGAAAGCAAAGGCAGAAATCACCAGGCTCATAAAAGAAGAGCTGATCCGG CTGCAAAATTCATACCAACCAACAAATAAAGGAAGATACAAAGTCTTATAGACATCCGGA AAAAAGATTTTTACCTGTGCTGGTCTATGATGTATGTGGCAGTTGCTGTCTGCAGTTTAC AATGTATTGTAAATGAAGATTTTTTAAATTCTATCTTGCTGATTTTTTTTAAATATAAGA AACTGGTACTTGGTAAAGAAATCTGTCCGTAAGTACCCCCACAATCAGTCAAACTATATT TAAAGCCAGCCTGTTTTCAGAGTATGATGTCCTTTAATGTAAACTCAAATATCAATATTT TAAATGTCCGGATAATATTCTAGAGGTTTAAAAAATGGAAATATTTGAACTTTCTATTGA AGACAATAAAGTACACAAGTCGTTAAGGGGCTATTCACTTTATCCTGTACTTTCAATGAA ATTGTGATCATTTCCTAAGAAAAGGTAAAATTCACTATCATATTTTGTGTCCCCACCTTG ATGTTACATGACTCTGGAACAATATGAACTGGATTTAAGAATGTTATAATAGAAGTCTTA CAAAATGGGTTGAGAGTTTTTGTTTCAATTTTTATCATCATAAATGGGCAAAAAGTAGTT GGACTAATTTCGGTTTTTATACAAGTAAAGATTTAATAGTAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_014829 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014829.2, NP_055644.2 |
| RefSeq Size | 5695 bp |
| RefSeq ORF | 3096 bp |
| Locus ID | 9879 |
| UniProt ID | Q7L014 |
| Domains | DEAD, helicase_C |
| Protein Pathways | Spliceosome |
| Gene Summary | This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) has an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202320 | DDX46 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46) |
CNY 7,176.00 |
|
| RC202320L3 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46), Myc-DDK-tagged |
CNY 10,740.00 |
|
| RC202320L4 | Lenti ORF clone of Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46), mGFP tagged |
CNY 10,740.00 |
|
| RG202320 | DDX46 (tGFP-tagged) - Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46) |
CNY 9,690.00 |
|
| SC114794 | DDX46 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 (DDX46) |
CNY 7,184.00 |
