SMG7 (NM_014837) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EST1C, SMG-7, SGA56M, C1orf16, FLJ23717, KIAA0250
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_014837, the custom clone sequence may differ by one or more nucleotides ATGAGCCTGCAGAGCGCGCAGTACCTCCGGCAGGCAGAAGTCCTGAAGGCTGACATGACAGATTCTAAGC TGGGTCCAGCTGAAGTCTGGACATCCAGGCAGGCTCTGCAGGACCTGTACCAGAAAATGCTAGTTACCGA TTTGGAATACGCTTTAGACAAGAAAGTAGAACAGGATCTCTGGAATCACGCCTTTAAGAATCAGATCACA ACACTGCAAGGCCAGGCAAAGAATCGAGCAAATCCGAATCGGAGTGAAGTTCAGGCAAACCTTTCTCTGT TCCTAGAGGCAGCTAGTGGCTTCTATACTCAGTTATTACAAGAACTGTGTACAGTATTTAATGTAGATTT ACCATGCCGTGTGAAGTCTTCCCAATTGGGAATTATCAGCAATAAACAGACGCATACCAGCGCCATAGTG AAGCCACAGTCTAGCTCCTGTTCCTATATCTGCCAGCACTGCCTCGTCCACCTTGGAGACATTGCTCGAT ACAGAAACCAGACCAGCCAGGCAGAGTCCTACTATAGGCATGCAGCTCAGCTTGTCCCCTCCAATGGTCA GCCTTATAATCAGTTGGCTATCTTAGCTTCTTCCAAAGGAGACCATCTGACCACAATTTTCTACTACTGC AGAAGCATTGCTGTGAAGTTCCCTTTCCCAGCTGCCTCCACTAATCTGCAAAAAGCACTTTCTAAAGCAC TGGAAAGCCGAGATGAGGTGAAAACCAAGTGGGGTGTTTCTGACTTCATCAAGGCCTTTATTAAATTCCA CGGTCATGTGTACCTGAGTAAGAGCTTGGAAAAGTTGAGCCCTCTTCGAGAGAAATTGGAAGAACAGTTT AAGAGGCTGCTATTCCAAAAAGCTTTCAACTCTCAGCAGTTAGTTCATGTCACTGTCATTAACCTGTTTC AACTTCATCACCTTCGTGACTTTAGCAATGAAACCGAGCAGCACACTTATAGCCAAGATGAGCAGCTATG TTGGACACAGTTGCTGGCCCTCTTTATGTCTTTTCTTGGCATCCTGTGCAAGTGTCCTCTACAGAATGAG TCTCAGGAGGAGTCCTACAATGCCTATCCTCTTCCAGCAGTCAAGGTCTCCATGGACTGGCTAAGACTCA GACCCAGGGTCTTTCAGGAGGCAGTGGTGGATGAAAGACAGTACATTTGGCCCTGGTTGATTTCTCTTCT GAATAGTTTCCATCCCCATGAAGAGGACCTCTCAAGTATTAGTGCGACACCACTTCCAGAGGAGTTTGAA TTACAAGGATTTTTGGCATTGAGACCTTCTTTCAGGAACTTGGATTTTTCCAAAGGTCACCAGGGTATTA CAGGGGACAAAGAAGGCCAGCAACGACGAATACGACAGCAACGCTTGATCTCTATAGGCAAATGGATTGC TGATAATCAGCCAAGGCTGATTCAGTGTGAAAATGAGGTAGGGAAATTGTTGTTTATCACAGAAATCCCA GAATTAATACTGGAAGACCCCAGTGAAGCCAAAGAGAACCTCATTCTGCAAGAAACATCTGTGATAGAGT CGCTGGCTGCAGATGGGAGCCCAGGGCTAAAATCAGTGCTATCTACAAGCCGAAATTTAAGCAACAACTG TGACACAGGAGAGAAGCCAGTGGTTACCTTCAAAGAAAACATTAAGACACGAGAAGTGAACAGAGACCAA GGAAGAAGTTTTCCTCCCAAAGTAACTCAAACCCCAACTCAAGCAAGTAACTCCCAGTTCATCCCCATTC ATCACCCTGGAGCCTTCCCTCCTCTTCCCAGCAGGCCAGGGTTTCCGCCCCCAACATATGTTATCCCCCC GCCTGTGGCATTTTCTATGGGCTCAGGTTACACCTTCCCAGCTGGTGTTTCTGTCCCAGGAACCTTTCTT CAGCCTACAGCTCACTCTCCAGCAGGAAACCAGGTGCAAGCTGGGAAACAGTCCCACATTCCTTACAGCC AGCAACGGCCCTCTGGACCAGGGCCAATGAACCAGGGACCTCAACAATCACAGCCACCTTCCCAGCAACC CCTTACATCTTTACCAGCTCAGCCAACAGCACAGTCTACAAGCCAGCTGCAGGTTCAAGCTCTAACTCAG CAACAACAATCCCCTACAAAAGCTGTGCCGGCTTTGGGGAAAAGCCCGCCTCACCACTCTGGATTCCAGC AGTATCAACAGGCAGATGCCTCCAAACAGCTGTGGAATCCCCCTCAGGTTCAAGGCCCATTAGGGAAAAT TATGCCTGTGAAACAGCCCTACTACCTTCAGACCCAAGACCCCATAAAACTGTTTGAGCCGTCATTGCAA CCTCCTGTAATGCAGCAGCAGCCTCTAGAAAAAAAAATGAAGCCTTTTCCCATGGAGCCATATAACCATA ATCCCTCAGAAGTCAAGGTCCCAGAATTCTACTGGGATTCTTCCTACAGCATGGCTGATAACAGATCTGT AATGGCACAGCAAGCAAACATAGACCGCAGGGGCAAACGGTCACCAGGAGTCTTCCGTCCAGAGCAGGAT CCTGTACCCAGAATGCCGTTTGAGAAATCCTTATTGGAGAAGCCCTCAGAGCTCATGTCACATTCATCCT CTTTCCTGTCCCTCACCGGATTCTCTCTCAATCAGGAAAGATACCCAAATAATAGTATGTTCAATGAGGT ATATGGGAAAAACCTGACATCCAGCTCCAAAGCAGAACTCAGTCCCTCAATGGCCCCCCAGGAAACATCT CTGTATTCCCTTTTTGAAGGGACTCCGTGGTCTCCATCACTTCCTGCCAGTTCAGATCATTCAACACCAG CCAGCCAGTCTCCTCATTCCTCTAACCCAAGCAGCCTACCCAGCTCTCCTCCAACACACAACCATAATTC TGTTCCATTCTCCAATTTTGGACCCATTGGGACTCCAGATAACAGGGATAGAAGGACTGCAGATCGGTGG AAAACTGATAAGCCAGCCATGGGTGGGTTTGGCATTGATTATCTCTCAGCAACGTCATCCTCTGAGAGCA GTTGGCATCAGGCCAGCACTCCGAGTGGCACCTGGACAGGCCATGGCCCTTCCATGGAGGATTCCTCTGC TGTCCTCATGGAAAGCCTAAAGTCTATCTGGTCCAGTTCCATGATGCATCCTGGACCTTCTGCTCTGGAG CAGCTGTTAATGCAGCAGAAGCAGAAACAGCAACGGGGACAAGGCACCATGAACCCTCCACACTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_014837 unedited CCCCATGTCCAGGAATTTGTCCTACGAACTCACTATAGGGCGGCCGCTGAATCGGCACGA GGTCTAGCTCCTGTTCCTATATCTGCCAGCACTGCCTCGTCCCCTTGGAGACTTGCTCGA ACAGAAACCAGACCAGCCAGGCAGAGTCCTACTATAGGCATGCAGCTCAGCTTGTCCCCT CCAATGGTCAGCCTTATAATCAGTTGGCTATCTTAGCTTCTTCCAAAGGAGACCATCTGA CCACAATTTTCTACTACTGCAGAAGCATTGCTGTGAAGTTCCCTTTCCCAGCTGCCTCCA CTAATCTGCAAAAAGCACTTTCTAAAGCACTGGAAAGCCGAGATGAGGTGAAAACCAAGT GGGGTGTTTCTGACTTCATCAAGGCCTTTATTAAATTCCACGGTCATGTGTACCTGAGTA AGAGCTTGGAAAAGTTGAGCCCTCTTCGAGAGAAATTGGAAGAACAGTTTAAGAGGCTGC TATTCCAAAAAGCTTTCAACTCTCAGCAGTTAGTTCATGTCACTGTCATTAACCTGTTTC AACTTCATCACCTTCGTGACTTTAGCAATGAAACCGAGCAGCACACTTATAGCCAAGATG AGCAGCTATGTTGGACACAGTTGCTGGCCCTCTTTATGTCTTTTCTTGGCATCCTGTGCA AGTGTCCTCTACAGAATGAGTCTCAGGAGGAGTCCTACAATGCCTATCCTCTTCCAGCAG TCAAGGTCTCCATGGACTGGCTAAGACTCAGACCCAGGGTCTTTCAGGAGGCAGTGGTGG ATGAAAGACAGTACATTTGGCCCTGGTTGACTTCTCTTCTGCATAGTTTCCATCCCCATG AAGAGGACCTCTCACGTATTAGTGCGACACCACTTCCAGAGGAGNTTGAATACAGGCATT TTTGGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_014837 unedited TTTAGCTCTGGACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTGTTGC ACTGCGAACATTTCGTGCACACTCAGCGGGCTGGTGGAATACTTACACCCTCTTGACAGC GAGAAACAGAAAAGAGGGCTGCCACCTGCAGGGGACTGTGTGGGCCAGTCCTGCAAACCC AACATGCCATGGTTTATGGAGCCTTCATTCCCAGGTTGCCACTTTGGCCTCAGTGTGGAG GGTTCATGGTGCCTTGTCCCCGTTGCTGTTTCTGCTTCTGCTGCATTAACAGCTGCTCCA GAGCAGAAGGTCCAGGATGCATCATGGAACTGGACCAGATAGACTTTAGGCTTTCCATGA GGACAGCAGAGGAATCCTCCATGGAAGGGCCATGGCCTGTCCAGGTGCCACTCGGAGTGC TGGCCTGATGCCAACTGCTCTCAGAGGATGACGTTGCTGAGAGATAATCAATGCCAAACC CACCCATGGCTGGCTTATCAGTTTTCCACCGATCTGCAGTCCTTCTATCCCTGTTATCTG GAGTCCCAATGGGTCCAAAATTGGAGAATGGAACAGAATTATGGTTGTGTGTTGGAGGAG AGCTGGGTAGGCTGCTTGGGTTAGAGGAATGAGGAGACTGGCTGGCTGGTGTTGAATGAT CTGAACTGGCAGGAAGTGATGGAGACCACGGAGTCCCTTCAAAAGGGAAACAGAGATGTT TCCTGGGGGGCCATTGAGGGACTGAGTTCTGCTTTGGAGCTGGATGTCANGTTTTTCCCA TATACCTCATTGAACATACTATTATTNTGGTATCTTTCCTGATTGAGAGAGATNNCGTGA GGNACANGNANAGAGATGAATGTGACATGAGCTCTGAGGGCTTCTCCATAAGATTAAAAA NNGCTCGTCAGAAGAGAGCGAGAGCCCCCACAGAGCGGTAAGATCAGAA
Restriction Sites	NotI-NotI
ACCN	NM_014837
Insert Size	3550 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_014837.1, NP_055652.1
RefSeq Size	5648 bp
RefSeq ORF	2409 bp
Locus ID	9887
Gene Summary	This gene encodes a protein that is essential for nonsense-mediated mRNA decay (NMD); a process whereby transcripts with premature termination codons are targeted for rapid degradation by a mRNA decay complex. The mRNA decay complex consists, in part, of this protein along with proteins SMG5 and UPF1. The N-terminal domain of this protein is thought to mediate its association with SMG5 or UPF1 while the C-terminal domain interacts with the mRNA decay complex. This protein may therefore couple changes in UPF1 phosphorylation state to the degradation of NMD-candidate transcripts. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2011]

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