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IST1 (NM_014761) Human Untagged Clone
CAT＃: SC114857
IST1 (untagged)-Human KIAA0174 (KIAA0174)
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CHMP8; OLC1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114857 sequence for NM_014761 edited (data generated by NextGen Sequencing)
ATGCTGGGCTCTGGATTTAAAGCTGAGCGCTTAAGAGTGAATTTGAGATTAGTCATAAAT CGCCTTAAACTATTGGAGAAAAAGAAAACGGAACTGGCCCAGAAAGCAAGGAAGGAGATT GCTGACTATCTGGCTGCTGGGAAAGATGAACGAGCTCGGATCCGTGTGGAGCACATTATC CGGGAAGACTACCTCGTGGAGGCCATGGAGATCCTGGAGCTGTACTGTGACCTGCTGCTG GCTCGGTTTGGCCTTATCCAGTCTATGAAGGAACTAGATTCTGGTCTGGCTGAATCTGTG TCTACATTGATCTGGGCTGCTCCTCGACTCCAGTCAGAAGTGGCTGAGTTGAAAATAGTT GCTGATCAGCTCTGTGCCAAGTATAGCAAGGAATATGGCAAGCTATGTAGGACCAACCAG ATTGGAACTGTGAATGACAGGCTAATGCACAAGCTGAGTGTGGAAGCCCCACCCAAAATC CTGGTGGAGAGATACCTGATTGAAATTGCAAAGAATTACAACGTACCCTATGAACCTGAC TCTGTGGTCATGGCAGAAGCTCCTCCTGGGGTAGAGACAGATCTTATTGATGTTGGATTC ACAGATGATGTGAAGAAAGGAGGCCCTGGAAGAGGAGGGAGTGGTGGCTTCACAGCACCA GTTGGTGGACCTGATGGAACGGTGCCAATGCCCATGCCCATGCCCATGCCTATGCCATCT GCAAATACGCCTTTCTCATATCCACTGCCAAAGGGACCATCAGATTTCAATGGACTGCCA ATGGGGACTTATCAGGCCTTTCCCAATATTCATCCACCTCAGATACCAGCAACTCCCCCA TCGTATGAATCTATGACATTAATGCTGATAAGAATATCTCTTCTGCACAGATTGTTGGTC CTGGACCCAAGCCAGAAGCCTCTGCAAAGCTTCCTTCCAGACCTGCAGATAACTATGACA ACTTTGTCCTACCAGAGTTGCCATCTGTGCCAGACACACTACCAACTGCATCTGCTGGTG CCAGCACCTCAGCATCTGAAGACATTGACTTTGATGATCTTTCCCGGAGGTTTGAAGAGC TGA Clone variation with respect to NM_014761.2 >OriGene 5' read for NM_014761 unedited
NGGGTGCAAATTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACCAGGCTGCGTT CACGGCAGGATTCGGTTAGGAGGAACAGCACAGCATGCTGGGCTCTGGATTTAAAGCTGA GCGCTTAAGAGTGAATTTGAGATTAGTCATAAATCGCCTTAAACTATTGGAGAAAAAGAA AACGGAACTGGCCCAGAAAGCAAGGAAGGAGATTGCTGACTATCTGGCTGCTGGGAAAGA TGAACGAGCTCGGATCCGTGTGGAGCACATTATCCGGGAAGACTACCTCGTGGAGGCCAT GGAGATCCTGGAGCTGTACTGTGACCTGCTGCTGGCTCGGTTTGGCCTTATCCAGTCTAT GAAGGAACTAGATTCTGGTCTGGCTGAATCTGTGTCTACATTGATCTGGGCTGCTCCTCG ACTCCAGTCAGAAGTGGCTGAGTTGAAAATAGTTGCTGATCAGCTCTGTGCCAAGTATAG CAAGGAATATGGCAAGCTATGTAGGACCAACCAGATTGGAACTGTGAATGACAGGCTAAT GCACAAGCTGAGTGTGGAAGCCCCACCCAAAATCCTGGTGGAGAGATACCTGATTGAAAT TGCAAAGAATTACAACGTACCCTATGAACCTGACTCTGTGGTCATGGCAGAAGCTCCTCC TGGGGTAGAGACAGATCTTATTGATGTTGGATTCACAGATGATGTGAAGAAAGGAGCCCC TGGAAGAGGANGGAGTGGTGGCTTCACAGCACCAGTTGGTGGACCTGATGGGACGGTGCC AATGCCCATGCCCATGCCCATGCCTATGCCATCTGCAAATACGCCTTTCTCATATNCACT GCCAAAGGGACCATCAGATTTCAATGGACTGGCCATGGGGACTTTATCAGCCTT >OriGene 3' read for NM_014761 unedited
TTGTACCGCGGCCGCATCTAAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAG GATCTTTAACTTTATTAGCAGCTACTGAGCTACAAAATACAAACCAACTGAAAACATTAA AAAAAGGCTGCCTGAAACAAGTATGTACAGGTATACTACACAGAAGCAAAAGCTGTATCA TCCAATCCCAAAGCCACCCCTGGGGGGAAGGATGACCTTACCTATGGGCCAAAGGAGTAA CAGAAGTTATGACATAAGAGTCCCATCCATCCAGGAAAGCAGGGAACAACTACAGCTGCA TGGGCACCAAAAATTTTTCTGAAATTAGTTTTAAAGGTTCCATGAATCAAAGTTTTCAAA TGAAAACAGGTACCCCTCTTTTCCTGTAGGCTTTCCAGCTCACTACCCAAAAGACTTGAG TACTTCTATTAAGGCAGCTGGAACCCCACCCTAAGACTTGAATGGCACCTTGCCCTTTCT CTGCCAGTAATGCAATCCAACACAATATGCTACAGGGAAAACAAAATTTCCACGGTGCCG CCCTCTGGTACAAGGGAAACAGCACGCAAAGCAAAAGGCCACAGAGGGCCTCCCTGAGAA TCCAGTACAACTAAGCGAGGCCTTCACCTGTCAAGACAAACTTTGCAACGTGGATCCGGG TGGGAACAGGGCCCTGCAGCGAGGAGTACTGGTTTTGCCAATGAGAGGGCCCACCTTCCA AGAACCCGAACATGGGCGTCCCGACGACTTTTCCCTTTCCCCCTTGCTTCCCAGAAGGGA AAAACCCCCAAGGTGGCCTTGATTCTCATTACCAACATACCCCTGGAAGAGGGGGTCTGT TTTCACTTCTGGTATCTCACACAAGGGTGGGATCCGTACAACGGCTCTTTTCCCCTGGTA AGACATTCTTGGGGTGTTGCCCCCCTCTTCCACCCCCCTTTGAACCTCCGCGCGGAGCTG TCGTACCGCACCCTTATCACCGCGCCACCCTTTTGTATTCCCACCCATACTCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014761 |
| Insert Size | 2310 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014761.2, NP_055576.2 |
| RefSeq Size | 2377 bp |
| RefSeq ORF | 1083 bp |
| Locus ID | 9798 |
| UniProt ID | P53990 |
| Domains | DUF292 |
| Gene Summary | This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012] Transcript Variant: This variant (2) uses an alternate splice site in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (b, also known as hIST1b) has a distinct C-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201347 | IST1 (Myc-DDK-tagged)-Human KIAA0174 (KIAA0174) |
CNY 3,656.00 |
|
| RC201347L1 | Lenti ORF clone of Human KIAA0174 (KIAA0174), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201347L2 | Lenti ORF clone of Human KIAA0174 (KIAA0174), mGFP tagged |
CNY 5,890.00 |
|
| RC201347L3 | Lenti ORF clone of Human KIAA0174 (KIAA0174), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201347L4 | Lenti ORF clone of Human KIAA0174 (KIAA0174), mGFP tagged |
CNY 5,890.00 |
|
| RG201347 | IST1 (tGFP-tagged) - Human KIAA0174 (KIAA0174) |
CNY 5,256.00 |
|
| SC324384 | IST1 (untagged)-Human KIAA0174 (KIAA0174) |
CNY 3,656.00 |
