United States
Germany
Japan
United Kingdom
China
EDEM1 (NM_014674) Human Untagged Clone
CAT＃: SC114903
EDEM1 (untagged)-Human ER degradation enhancer, mannosidase alpha-like 1 (EDEM1)
CNY 7,344.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EDEM |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_014674, the custom clone sequence may differ by one or more nucleotides
ATGCAATGGCGAGCGCTCGTCCTGGGGCTGGTGCTCCTCCGGCTTGGCCTCCATGGAGTATTGTGGCTCG TCTTCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGCTTTCCGCTCAGCTTCGGCTTCCAGCGTCTGAG GAGCCCCGACGGCCCCGCGTCGCCCACCTCGGGGCCCGTGGGCCGGCCTGGGGGGGTATCCGGGCCGTCG TGGCTGCAGCCGCCGGGGACCGGGGCAGCGCAGAGCCCGCGCAAGGCTCCGCGGCGTCCTGGGCCGGGGA TGTGCGGCCCAGCCAACTGGGGCTACGTGCTGGGCGGCCGGGGCCGCGGCCCGGACGAGTACGAGAAGCG CTACAGCGGCGCCTTCCCTCCGCAGCTGCGTGCCCAGATGCGCGACCTGGCACGGGGCATGTTCGTCTTT GGCTACGACAACTACATGGCTCACGCCTTCCCCCAGGACGAGCTCAACCCCATCCACTGCCGCGGCCGTG GGCCCGACCGCGGGGACCCTTCAAATCTGAACATCAATGATGTACTAGGGAACTACTCATTGACTCTTGT TGATGCATTGGATACACTTGCAATAATGGGAAATTCATCCGAGTTCCAGAAAGCCGTCAAGTTAGTGATC AACACAGTTTCATTTGACAAAGATTCCACCGTCCAAGTCTTTGAGGCCACGATAAGGGTCCTGGGAAGCC TCCTTTCTGCTCACAGAATAATAACTGACTCCAAGCAGCCCTTTGGTGACATGACAATTAAGGACTATGA TAATGAGTTGTTATACATGGCCCATGACCTGGCGGTGCGGCTCCTCCCTGCTTTTGAAAACACCAAGACA GGGATTCCATATCCTCGGGTGAATCTAAAGACAGGAGTTCCTCCTGACACCAATAATGAGACATGCACAG CGGGAGCCGGTTCCCTCCTGGTGGAATTTGGGATTCTGAGTCGACTCCTGGGGGACTCCACATTTGAGTG GGTGGCCAGACGAGCAGTGAAAGCCCTTTGGAACCTCCGGAGCAATGATACAGGATTACTAGGCAATGTC GTGAACATTCAGACGGGCCACTGGGTTGGAAAGCAGAGTGGCCTGGGTGCCGGGCTGGACTCCTTCTATG AATACCTCTTGAAATCTTACATTCTCTTTGGAGAAAAAGAAGACCTAGAAATGTTTAATGCTGCATATCA GAGTATTCAGAACTACTTAAGAAGAGGGCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCTCCACTCTAT GTCAACGTGAACATGTTCAGTGGGCAGCTGATGAACACCTGGATTGACTCTCTGCAGGCCTTTTTCCCTG GACTGCAGGTGCTGATAGGAGATGTGGAAGATGCCATCTGCCTTCATGCCTTCTACTATGCCATATGGAA ACGATATGGTGCCCTCCCTGAGAGATATAACTGGCAGCTGCAGGCCCCTGACGTTCTCTTCTACCCACTG AGACCAGAGTTAGTGGAATCCACATATCTCCTCTACCAGGCAACCAAGAATCCCTTCTACCTCCATGTAG GAATGGATATTCTGCAGAGTCTGGAAAAGTACACAAAAGTCAAGTGTGGGTACGCCACGCTGCATCACGT CATTGACAAGTCCACAGAAGACCGGATGGAGAGCTTCTTTCTCAGTGAGACCTGTAAATATTTGTATCTG CTGTTTGATGAAGACAATCCAGTACACAAGTCTGGAACCAGATACATGTTCACAACAGAGGGACACATTG TATCTGTGGATGAGCATCTTCGGGAATTGCCATGGAAGGAATTCTTCTCTGAAGAGGGAGGGCAGGACCA AGGGGGAAAGTCTGTGCACAGGCCGAAACCTCATGAGTTAAAAGTCATCAACTCCAGCTCCAACTGCAAT CGTGTACCTGATGAGAGGAGGTACTCCCTGCCCTTAAAGAGCATCTACATGCGACAGATTGACCAGATGG TTGGTTTGATTTGA >OriGene 5' read for NM_014674 unedited
TCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAG AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA TTCGGCACGAGGGGCCGGGCCAATCCCGGGCCACTGGGCGGGCGACCGTGGGGCCTGGCC GGCCCGCCGGAGAGCCTGGGGGTTGGGGACGATGGGCTAGTTCCTGGTGCGTCACGGGGG AGTTCCTTAAAGGGGAAGCGAGCCGGGCTACGGGGCGAGCGCGGGGTGCGGTGGTCGGCG GGGAGGCCCCCGCGCTTTAAAATAATGCCCGCGGCGCCCGCGCGACCATGCAATGGCGAG CGCTCGTCCTGNGGCTGGTGCTCCTCCGGCTTGGCCTCCATGGAGTATTGTGGCTCGTCT TCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGCTTTCCGCTCAGCTTCGGCTTCCAGC GTCTGAGGAGCCCCGACGGCCCCGCGTCGCCCACCTCGGGGCCCGTGNGCCGGCCTGNNG GGGTATCCGGNCCGTCGTGGCTGCAGCCGCCGGNGACCGGNGCAGCGCAGAGCCCGCGCA AGGGGCTCGCGCGTCCTGGGCCGGGGATGTGCGGCCCAGCCACTGGNGCTACNNTGCTGG CGGCCGGGCCCGCNGCCCGGACGAGTACGAGAAGCGCTACAGCGGGCGCCTCCCTNCGCA GCTGCGTGCCCAGATGCGCGACCTGCCACGGGGCATGGTCGTCTTTGGCTACAAACACTA CATGGTCACGCCTTNCCCCCAGACGAGCTCAACCCCTCCACTGCCCGCGCCCGTGGCCCC GACGGGGGGGACCCTTTCAATCTTGACATCATGTGTACTAGGGAACTACTCCTGCCTCTG TTGAAGCATTGGAACCCTGCCATATGGGAATTTTTCCGATTCGAAAGC >OriGene 3' genomic read for NM_014674 unedited
CNANGATCGGTTTTTTTTTTTTTTTTTTGGAAACTCAAAAAAAGAATCTTTAAAAAATTA CTCTAAAATCCTAAAATTTTAAAGAGCAGATTTCCTTATTACATTTATAAAAGAACATTT GGTCCTTTTACAAAAAGATCCCTTTTAATTTAAATACATTTCTTATTTACAGATTAAACA TAAATATCATCTACAGTTGCAAAGCATATTGCACATTACAGAGAAGCATTTGTGTATTTC CGTAAGTTTTCCCAGAGGTTCCAACTCTATACTTTTTTTGTAAAAGATTTACCTTTCGTA TGCATCATAATTAAAAATGCAGCTTGTGTTCTGCTGATTAAAACTGATGACAGAAGCCCG TGCAAAAGCACTATTCCTCTGCCTTGGATGCGTTAGAAGAGGTAAGCCGGCTGTGGTGGC TCAGACCTGTGATCCTACCACTTGAGGAGGCCCAAGAGTCCGGGTCAAGAGGTCCAGAGA TTCCGATCATCTGGGCAGCATGGGGAAAACCCCTCTCTACTAGGGACTAAAAGATTAGCT GGGCCTGGTGGTGCATGCGTGGGGTCCCCAGGTTCGTCAGAGGGGTGAGGCAAAGAAATT CCCCCTGACCCGGGAAGGCTGAGTTTGCAGTGGGGCCAGGATCACGCCATTGGAGAGAAA CCCAAGCCCCAGGGGCAGGAATTCGGTCCCATATGGCGGACGGTGGCGGGGTGGGTTGTT TGCCCGCTGGGGGATCTGGGAATATACCAGGTGCTTCGGAGGGATTAAACGGCGCATTCT GCTGTTCAGGCGGGGAATGGGCCCGTGACGCGAGGGTGCGCGCCTAATTCAAATCCCGGG CCGCTAATACAGAGGTTGCCGGGCTAAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014674 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014674.1, NP_055489.1 |
| RefSeq Size | 6072 bp |
| RefSeq ORF | 1974 bp |
| Locus ID | 9695 |
| UniProt ID | Q92611 |
| Domains | Glyco_hydro_47 |
| Protein Families | Transmembrane |
| Gene Summary | Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205655 | EDEM1 (Myc-DDK-tagged)-Human ER degradation enhancer, mannosidase alpha-like 1 (EDEM1) |
CNY 7,336.00 |
|
| RC205655L3 | Lenti ORF clone of Human ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC205655L4 | Lenti ORF clone of Human ER degradation enhancer, mannosidase alpha-like 1 (EDEM1), mGFP tagged |
CNY 6,460.00 |
|
| RG205655 | EDEM1 (tGFP-tagged) - Human ER degradation enhancer, mannosidase alpha-like 1 (EDEM1) |
CNY 8,936.00 |
