MAN1 (LEMD3) (NM_014319) Human Untagged Clone

Name: MAN1 (LEMD3) (NM_014319) Human Untagged Clone
SKU: SC115036
Price: 6680.00

CAT＃: SC115036

LEMD3 (untagged)-Human LEM domain containing 3 (LEMD3), transcript variant 1

Datasheet

SDS

"NM_014319" in other vectors (6)

CNY 6,680.00

货期*

现货

规格

Cited in 1 publication.

Product images

经常一起买 (4)

TA500797

Anti-LEMD3 (MAN1) mouse monoclonal antibody, clone OTI4E1 (formerly 4E1)

CNY 1,999.00
CNY 2,700.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MAN1
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_014319, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGGCAGCAGCTTCGGCGCCTCAGCAGCTCTCGGATGAGGAGCTTTTCTCTCAGCTCCGCCGTT ACGGCCTGTCTCCCGGACCAGTGACGGAGAGCACCCGCCCGGTCTACCTCAAGAAGCTGAAGAAGCTTCG AGAGGAAGAGCAGCAACAGCACCGGTCAGGGGGCCGCGGCAACAAGACGCGGAACAGTAATAACAATAAC ACGGCAGCCGCCACGGTCGCAGCCGCGGGACCAGCGGCGGCGGCGGCCGCGGGGATGGGGGTCCGGCCGG TCTCGGGCGACCTCTCCTACTTACGGACTCCTGGGGGCCTGTGCCGAATCTCGGCCTCTGGCCCAGAGAG CCTCCTGGGAGGGCCCGGGGGCGCCTCCGCCGCCCCCGCGGCTGGCAGCAAAGTGCTGCTGGGCTTCAGC TCGGACGAGTCGGACGTGGAGGCCAGTCCCCGGGACCAGGCCGGCGGCGGCGGGAGGAAAGACCGGGCTT CGCTCCAGTACCGCGGGCTCAAAGCGCCGCCGGCGCCCCTGGCCGCCAGCGAGGTGACTAACAGCAACTC TGCAGAGCGAAGGAAGCCCCACTCGTGGTGGGGGGCCAGGAGGCCGGCGGGCCCCGAGCTGCAGACCCCG CCGGGGAAAGATGGAGCAGTGGAGGACGAGGAAGGGGAGGGAGAGGACGGTGAGGAGAGGGACCCGGAGA CCGAGGAGCCGCTCTGGGCGAGCCGGACCGTGAATGGCAGCCGGCTTGTCCCCTACAGCTGCCGGGAAAA CTATTCGGACTCAGAGGAAGAGGACGACGACGACGTGGCCTCCAGCAGACAGGTATTAAAGGACGACTCC CTTTCCCGGCATCGGCCCAGACGAACCCATAGTAAGCCTCTCCCCCCGCTGACTGCTAAATCGGCCGGCG GCAGGCTGGAGACTTCAGTTCAGGGAGGGGGAGGACTCGCGATGAATGACAGGGCGGCGGCTGCCGGGAG TCTAGACAGGAGCCGAAACCTCGAAGAGGCGGCGGCCGCGGAGCAGGGAGGAGGGTGTGATCAAGTGGAC TCCAGCCCCGTTCCTAGATACCGTGTTAACGCTAAGAAACTGACCCCTCTCCTGCCCCCGCCACTTACTG ACATGGACTCAACCTTGGATTCGTCAACAGGCTCCCTTCTGAAAACCAATAATCATATTGGCGGTGGGGC CTTCAGTGTGGACTCCCCCAGGATTTATTCTAACAGTCTCCCTCCCAGTGCGGCGGTGGCCGCCTCTAGT TCACTCAGGATCAATCACGCCAATCATACGGGCTCCAATCATACCTACCTGAAAAACACATACAACAAAC CGAAGCTTTCCGAACCCGAAGAGGAACTTCTCCAGCAATTTAAACGGGAGGAGGTGTCCCCAACAGGGAG TTTCAGTGCCCACTACTTGTCGATGTTTCTCTTAACTGCTGCCTGCTTATTTTTCCTAATACTGGGACTG ACTTACCTAGGAATGAGAGGGACAGGAGTATCTGAGGATGGAGAACTCAGCATAGAAAACCCCTTTGGTG AAACATTTGGAAAAATACAAGAAAGTGAAAAAACTCTTATGATGAACACATTATATAAGCTTCATGATCG ATTGGCACAGCTTGCAGGAGATCATGAATGTGGCAGTTCTAGTCAAAGAACGCTTTCTGTTCAAGAGGCA GCTGCGTATTTAAAAGATTTAGGTCCTGAATATGAAGGTATATTTAACACTTCATTGCAGTGGATCTTAG AAAATGGAAAAGATGTTGGAATAAGGTGTGTTGGTTTTGGCCCTGAGGAAGAATTGACAAATATAACTGA TGTGCAGTTTTTACAGTCCACAAGACCACTGATGTCTTTTTGGTGTCGTTTTCGACGTGCTTTTGTTACT GTAACTCACAGATTATTGTTGTTATGCTTAGGTGTAGTGATGGTTTGTGTCGTTCTGCGTTACATGAAAT ATCGATGGACAAAAGAAGAGGAGGAAACAAGGCAGATGTATGATATGGTGGTAAAGATTATAGATGTTTT ACGAAGTCATAATGAAGCCTGCCAGGAAAACAAAGATTTACAACCTTACATGCCTATTCCACATGTACGC GATTCCTTAATACAGCCTCATGACAGGAAAAAAATGAAGAAAGTCTGGGATAGAGCTGTTGACTTCCTTG CTGCTAATGAGTCTAGAGTTCGCACGGAAACACGAAGAATAGGTGGTGCAGATTTTCTGGTTTGGCGGTG GATCCAGCCTTCTGCATCCTGTGACAAAATATTAGTTATACCTTCTAAAGTATGGCAAGGTCAAGCATTT CATTTAGATAGAAGAAATTCACCACCAAATAGTTTGACACCGTGTCTAAAGATTCGGAATATGTTTGATC CCGTTATGGAAATAGGGGATCAGTGGCATTTGGCAATTCAAGAAGCAATTTTAGAAAAATGCAGTGATAA TGATGGCATTGTTCACATTGCAGTAGACAAAAATTCACGTGAGGGTTGTGTATATGTTAAATGTCTGTCT CCAGAATATGCTGGAAAGGCTTTTAAAGCATTGCATGGCTCTTGGTTTGATGGGAAATTGGTTACAGTAA AATATTTACGACTAGATAGATACCACCATCGCTTTCCCCAGGCTCTCACTTCCAACACTCCATTGAAGCC ATCAAATAAACATATGAACTCCATGTCTCATCTTCGTCTTCGGACTGGCCTAACCAATTCTCAAGGAAGT TCCTGA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_014319 unedited NGGGTCGGAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGAGAAAAG GCGGCGGCAGCAGCTTCGGCGCCTCAGCAGCTCTCGGATGAGGAGCTTTTCTCTCAGCTC CGCCGTTACGGCCTGTCTCCCGGACCAGTGACGGAGAGCACCCGCCCGGTCTACCTCAAG AAGCTGAAGAAGCTTCGAGAGGAAGAGCAGCAACAGCACCGGTCAGGGGGCCGCGGCAAC AAGACGCGGAACAGTAATAACAATAACACGGCAGCCGCCACGGTCGCAGCCGCGGGACCA GCGGCGGCGGCGGCCGCGGGGATGGGGGTCCGGCCGGTCTCGGGCGACCTCTCCTACTTA CGGACTCCTGGGGGCCTGTGCCGAATCTCGGCCTCTGGCCCAGAGAGCCTCCTGGGAGGG CCCGGGGGCGCCTCCGCCGCCCCCGCGGCTGGCAGCAAAGTGCTGCTGGGCTTCAGCTCG GACGAGTCGGACGTGGAGGCCAGTCCCCGGGACCAGGCCGGCGGCGGCGGGAGGAAAGAC CGGGCTTCGCTCCAGTACCGCGGGCTCAAAGCGCCGCCGGCGCCCCTGGCCGCCAGCGAG GTGACTAACAGCAACTCTGCAGAGCGAAGGAAGCCCCACTCGTGGTGGGGGGCCAGGAGG CCGGCGGGCCCCGAGCTGCAGACCCCGCCGGNGAAAGATGGAGCAGTGGAGGACGAAGGA AGGGGAGGGAGAGGACGGTGAGGAGAGGGACCCGGNAGACCGAGAGCCGCTCTGGGCGAG CCGGACCGTGAATGGCAGCCGGCTTGTCCCCTACAGCTGCCGGNGAAACTATTCGNACTC AGAGGAAAGAGACGACGACGACGTGGCCTTCAGCAGACAGGTATTAAAGGACGACTNCCT TTTCCGGNCATCGNCCAGACGAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_014319 unedited ATTTGGCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTAAAACTA ATAAATTTTTAATTAGAATTTTTTCCTTTTTTTAACAGGACAAGTAACAGATTACATCAA ACTTCAGAACTTCTCAAATACCTAGTTATTATACACATTCCCATCTGTCTTGCAAGGAGG GATCTTGGTCGGCTTAACAGTTTCAGGTAAAATAAGCTGCATAATAGTATATATTACAAT AATAAATGTACAATGTTTACATGAAATATGTTTTAGAAGCAAGAAAAAGATGCATCTGCA TCAAAGGCGACCTTTTAAAATGCCACAACTATTTTTTACATTCACTCTTGCAACAGGTTA CAAGATGGGGGTCACTAAGCTCAGATAAATCACTATGATGCACATAAAATACAGGAAGAC TGGTTACTTTATATATTATATATATTTATATTTTCACACGTTAGCATCTGAGACATAAAC TGAGAATTTAAAATTTATTCTCTTCAGATAACAAATCATTTTTTATTCTTCATAAACAAG GTGCCCACAAAAGCACACAGGTAAGGCACAATAGGGCTTTCTGTTACAGAATAGTGTGCA TCATGTTTACTTAACCAGAAAACGTGCATTATCTGTGCAAGTTCATGCTGCAAACCCAAC GCATACAATAAAACAAAAAGGAAACTGAGCTTTAAATACAACCAAAATTTGTACAATTCC AGCTGTTTCACATATTCATTTGGACTGANCATTATATATCAACAGGTTATTTTTAAAAGC CAATTGTAGATTACAAGTAAAATTTACTCAGCAACTATTTTTATCCTGAATCATAAACAG TNNATAAGTAAGTTTACTGAAACAAATTAATACTTGAGCACANAGCACAGTCCATCGTGC ATTTACTATATTGCAAACTTTATGACCCCAAATGCAAGTTAATTTGTCAAAACACATTTG CTGAA
Restriction Sites	NotI-NotI
ACCN	NM_014319
Insert Size	4140 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_014319.3, NP_055134.2
RefSeq Size	4785 bp
RefSeq ORF	2736 bp
Locus ID	23592
UniProt ID	Q9Y2U8
Domains	LEM
Protein Families	Transmembrane
Gene Summary	This locus encodes a LEM domain-containing protein. The encoded protein functions to antagonize transforming growth factor-beta signaling at the inner nuclear membrane. Two transcript variants encoding different isoforms have been found for this gene. Mutations in this gene have been associated with osteopoikilosis, Buschke-Ollendorff syndrome and melorheostosis.[provided by RefSeq, Nov 2009] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this cDNA Clones has been cited in the following citations:
Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus ,null, Nature Communications ,PubMed ID 32358486 [LEMD3]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC212759	LEMD3 (Myc-DDK-tagged)-Human LEM domain containing 3 (LEMD3), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,672.00 CNY 9,600.00
RC212759L1	Lenti ORF clone of Human LEM domain containing 3 (LEMD3), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,072.00
RC212759L2	Lenti ORF clone of Human LEM domain containing 3 (LEMD3), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 11,500.00
RC212759L3	Lenti ORF clone of Human LEM domain containing 3 (LEMD3), transcript variant 1, Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 11,500.00
RC212759L4	Lenti ORF clone of Human LEM domain containing 3 (LEMD3), transcript variant 1, mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 11,500.00
RG212759	LEMD3 (tGFP-tagged) - Human LEM domain containing 3 (LEMD3), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,272.00

其它MAN1产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies