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PI 3 Kinase p85 alpha (PIK3R1) (NM_181523) Human Untagged Clone
CAT＃: SC115320
PIK3R1 (untagged)-Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1
CNY 7,968.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AGM7; GRB1; IMD36; p85; p85-ALPHA |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_181523 edited
ATGAGTGCTGAGGGGTACCAGTACAGAGCGCTGTATGATTATAAAAAGGAAAGAGAAGAA GATATTGACTTGCACTTGGGTGACATATTGACTGTGAATAAAGGGTCCTTAGTAGCTCTT GGATTCAGTGATGGACAGGAAGCCAGGCCTGAAGAAATTGGCTGGTTAAATGGCTATAAT GAAACCACAGGGGAAAGGGGGGACTTTCCGGGAACTTATGTAGAATATATTGGAAGGAAA AAAATCTCGCCTCCCACACCAAAGCCCCGGCCACCTCGGCCTCTTCCTGTTGCACCAGGT TCTTCGAAAACTGAAGCAGATGTTGAACAACAAGCTTTGACTCTCCCGGATCTTGCAGAG CAGTTTGCCCCTCCTGACATTGCCCCGCCTCTTCTTATCAAGCTCGTGGAAGCCATTGAA AAGAAAGGTCTGGAATGTTCAACTCTATACAGAACACAGAGCTCCAGCAACCTGGCAGAA TTACGACAGCTTCTTGATTGTGATACACCCTCCGTGGACTTGGAAATGATCGATGTGCAC GTTTTGGCTGACGCTTTCAAACGCTATCTCCTGGACTTACCAAATCCTGTCATTCCAGCA GCCGTTTACAGTGAAATGATTTCTTTAGCTCCAGAAGTACAAAGCTCCGAAGAATATATT CAGCTATTGAAGAAGCTTATTAGGTCGCCTAGCATACCTCATCAGTATTGGCTTACGCTT CAGTATTTGTTAAAACATTTCTTCAAGCTCTCTCAAACCTCCAGCAAAAATCTGTTGAAT GCAAGAGTACTCTCTGAAATTTTCAGCCCTATGCTTTTCAGATTCTCAGCAGCCAGCTCT GATAATACTGAAAACCTCATAAAAGTTATAGAAATTTTAATCTCAACTGAATGGAATGAA CGACAGCCTGCACCAGCACTGCCTCCTAAACCACCAAAACCTACTACTGTAGCCAACAAC GGTATGAATAACAATATGTCCTTACAAGATGCTGAATGGTACTGGGGAGATATCTCGAGG GAAGAAGTGAATGAAAAACTTCGAGATACAGCAGACGGGACCTTTTTGGTACGAGATGCG TCTACTAAAATGCATGGTGATTATACTCTTACACTAAGGAAAGGGGGAAATAACAAATTA ATCAAAATATTTCATCGAGATGGGAAATATGGCTTCTCTGACCCATTAACCTTCAGTTCT GTGGTTGAATTAATAAACCACTACCGGAATGAATCTCTAGCTCAGTATAATCCCAAATTG GATGTGAAATTACTTTATCCAGTATCCAAATACCAACAGGATCAAGTTGTCAAAGAAGAT AATATTGAAGCTGTAGGGAAAAAATTACATGAATATAACACTCAGTTTCAAGAAAAAAGT CGAGAATATGATAGATTATATGAAGAATATACCCGCACATCCCAGGAAATCCAAATGAAA AGGACAGCTATTGAAGCATTTAATGAAACCATAAAAATATTTGAAGAACAGTGCCAGACC CAAGAGCGGTACAGCAAAGAATACATAGAAAAGTTTAAACGTGAAGGCAATGAGAAAGAA ATACAAAGGATTATGCATAATTATGATAAGTTGAAGTCTCGAATCAGTGAAATTATTGAC AGTAGAAGAAGATTGGAAGAAGACTTGAAGAAGCAGGCAGCTGAGTATCGAGAAATTGAC AAACGTATGAACAGCATTAAACCAGACCTTATCCAGCTGAGAAAGACGAGAGACCAATAC TTGATGTGGTTGACTCAAAAAGGTGTTCGGCAAAAGAAGTTGAACGAGTGGTTGGGCAAT GAAAACACTGAAGACCAATATTCACTGGTGGAAGATGATGAAGATTTGCCCCATCATGAT GAGAAGACATGGAATGTTGGAAGCAGCAACCGAAACAAAGCTGAAAACCTGTTGCGAGGG AAGCGAGATGGCACTTTTCTTGTCCGGGAGAGCAGTAAACAGGGCTGCTATGCCTGCTCT GTAGTGGTGGACGGCGAAGTAAAGCATTGTGTCATAAACAAAACAGCAACTGGCTATGGC TTTGCCGAGCCCTATAACTTGTACAGCTCTCTGAAAGAACTGGTGCTACATTACCAACAC ACCTCCCTTGTGCAGCACAACGACTCCCTCAATGTCACACTAGCCTACCCAGTATATGCA CAGCAGAGGCGATGA >OriGene 5' read for NM_181523 unedited
AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCGGAGTTG GAGGAAGCAGCGGCAGCGGCGAGGGCGGCAGGCTAGCTGTCGGAGACGGCAAGCACGGAT CGGGCACGGCCGGCGGGTCGCAGCCGGGCTGGAGGCCGGTCGGAACCGAGCGGGCAGGAG GCCACCGCTGCAGCGCGGGCCCCGCAGAGGAAGGAAGCCGGCGGGCGGGGTAGGTGAAGC TCGTGTGTGGAGTGCCACGGTACAATCAGACGACAGATGGACAGTGTGACAAAAGTGTCA GAAAGGATTGGGCCTCGCTGTGAGAGTCAGCCTGGATTCAAAGTGTTGACAAGTTGCTGA AAAGGAAGCCAGTGAGAGGACTGTGGCACGCAGAGGAAGTGGAGCCCTGTCTTCGGTCAC ACCATTGATGGAGGACAGATGGACAGCCGTATGGCCAGTCACCTCTCCTCTTAAACCTTT GGAGAGTGGTCCTTTGTCCTCTGCTGGACACATAATAGGAATTCTAACACATTCTCTGAA TTCACTTTTCATAAAAACGTAAAATCAGACTGCTCTGTACAACCAGGCTCAACTGTTGCA TGGTAGCAGATTTGCAAACATGAGTGCTGAGGGGTACCAGTACAGAGCGCTGTATGATTA TAAAAAGGAAAGAGAAGAAGATATTGACTTGCACTTGGGTGACATATTGACTGTGAATAA AGGGTCCTTAGTAGCTCTTGGATTCAGTGATGGACAGGAAGCCCAGCCTGTAGAANATGG CTGGTTAAATGGCTATAATGAAACCACAGGGGAAAGGGGGGACTNTCCCGGGACTTATGT AGGATATATTGGAAGGAAAAAAATCTCGCCTTCCACACCAAAGCCCCGGCCACCTCGGCT CTTNNCTGTGCACCAGTTCTTCGAAACTGAAGCAGATGTTGACAACAGCTTTGN >OriGene 3' read for NM_181523 unedited
NNNTTTTACTTTGAACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTAA ATATTTCATAGTTTTAATTCTGAAGCCATTTTTTTTTTTTACTGGCATCCTGTACATTTA CTTTTAAAAAAGGATAACAAAAATGAATATTAACAAAAATCCGGGACAACAATATTTTCA AGCAACAAAAACTGGGGTGGGGAAGCTTATTCTGAAGGTACATTTAAAACTGAAATAACA ACTTAATGAAAATTAAAAATTGCATAGCGCTGTGAATTTAGCCTTCAGCAAAACAAAACA GAAGCTATTTGGTATTGATACAAATCCATCTATTTGATAGTTAGTCATCCAATATTATGT ACATATTTTATATACTGAATGTCATTTTAAGTCCTGTTTTCCAAACTCCATTTTTCTGTT GCTGGGTTTTTGTTTTTTGACAAGTTAAACACTTTCTGGCACTTTCTATGACAGAATTTC TTCTGAACATACATGAACTGACATTCTCCCAAAGCGTCCCTTGTGAGTGGACGTGCCTTT CTGCTACATATCGTTCATTTGTTACAAAATGAAATAATCCACAGTGCGATGTGTCTGGGT CCACCGTGCACAGCAACATCCAGGCTAAACCAGGCTGGACCAAACCTTCTCAGGCCAGGG TTCTTNTGCCTCTCTCTCCTCCGCTCTCCAGAGTCCCAACTTTCAGCTGGTAAAGCTTCA GAAAGCATTAACAAGCACCATGGTTAAAGGCCTCTTTGTCCCTGCCGCAGAGATTTTGCA TTTCTTTTCTCTTTGNGTGNATGGGGGNTGGGGCTTTAAATAAACCAAAGAAAAAGAAAA GAAGGATAAGCACTCACTTAGAACGTGTGTCATCTACACCTTAGCTGAATGGCTTCCCTA CTTT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_181523 |
| Insert Size | 3890 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181523.1, NP_852664.1 |
| RefSeq Size | 6453 bp |
| RefSeq ORF | 2175 bp |
| Locus ID | 5295 |
| UniProt ID | P27986 |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway |
| Gene Summary | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A high-throughput immobilized bead screen for stable proteins and multi-protein complexes
,Meghan A. Lockard, Pawel Listwan, Jean-Denis Pedelacq, Stéphanie Cabantous, Hau B. Nguyen, Thomas C. Terwilliger, and Geoffrey S. Waldo,
Protein Eng. Des. Sel., Jun 2011; 10.1093/protein/gzr021
[PIK3R1]
|
|
A high-throughput immobilized bead screen for stable proteins and multi-protein complexes
,null,
Protein Engineering, Design and Selection
,PubMed ID 21642284
[PIK3R1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210544 | PIK3R1 (Myc-DDK-tagged)-Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1 |
CNY 7,952.00 |
|
| RC210544L1 | Lenti ORF clone of Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1, Myc-DDK-tagged |
CNY 10,352.00 |
|
| RC210544L2 | Lenti ORF clone of Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC210544L3 | Lenti ORF clone of Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1, Myc-DDK-tagged |
CNY 10,352.00 |
|
| RC210544L4 | Lenti ORF clone of Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1, mGFP tagged |
CNY 10,352.00 |
|
| RG210544 | PIK3R1 (tGFP-tagged) - Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1 |
CNY 9,552.00 |
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